Status
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Gene information
General information
TC014208 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3197996..3205616
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014208
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10137

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014208 RA
      MNTQVVSRE RSIFLSKLS FPTSSSDSR LQDEGNNSD 
      VEGKVKNRL LSMWNNVKY GMKLKTNFS KESPVWLLG 
      KCYRRIESP SSDSTELGT DVAAFQSQS EIASSDDEG 
      FEGFKKDFI SRLWLTYRR EFPILNGSN YSSDCGWGC 
      MLRSGQMLI AQALVCHIL GRDWRWQPD HQPTTRESF 
      IEVVNHRKI IKWFGDKPS RNSPFSIHT LVALGEASG 
      KKAGDWYGP GFVAHLFRQ AFKRASEDN YEFDSLTVC 
      VAQDCAVYI KDVMEECTD KNGKWKSLI LLIPVRLGA 
      EKFNSIYAP CLTTLFSLK QCIGIIGGR PKHSLYFVG 
      YQDDKLIHL DPHYCQEVV DVWAVDFPL TSFHCRSPR 
      KIHLSKMDP SCCIGFYCP TKESFFNLI EIVQPLVIP 
      PGRTDSPGS EYPMFIFCD GYSRDAQLS PKKLPPSEL 
      EYSMDSSTD GDDMGTEAF EIL
    • >TC014208 RB
      MNTQDEGNN SDVEGKVKN RLLSMWNNV KYGMKLKTN 
      FSKESPVWL LGKCYRRIE SPSSDSTEL GTDVAAFQS 
      QSEIASSDD EGFEGFKKD FISRLWLTY RREFPILNG 
      SNYSSDCGW GCMLRSGQM LIAQALVCH ILGRDWRWQ 
      PDHQPTTRE SFIEVVNHR KIIKWFGDK PSRNSPFSI 
      HTLVALGEA SGKKAGDWY GPGFVAHLF RQAFKRASE 
      DNYEFDSLT VCVAQDCAV YIKDVMEEC TDKNGKWKS 
      LILLIPVRL GAEKFNSIY APCLTTLFS LKQCIGIIG 
      GRPKHSLYF VGYQDDKLI HLDPHYCQE VVDVWAVDF 
      PLTSFHCRS PRKIHLSKM DPSCCIGFY CPTKESFFN 
      LIEIVQPLV IPPGRTDSP GSEYPMFIF CDGYSRDAQ 
      LSPKKLPPS ELEYSMDSS TDGDDMGTE AFEIL
    CDS sequence
    • >TC014208 RA
      atgaacaca caggtggta tcaagagag cgatcaatt 
      tttttgtcc aaacttagc ttcccaaca agttcttct 
      gacagtcgt ttgcaggat gaagggaac aattcggac 
      gtcgaaggc aaagtcaaa aacagactc ctctcaatg 
      tggaataac gtgaaatat gggatgaag ctcaagacg 
      aacttctcg aaagaatct cccgtctgg ctcttgggc 
      aagtgctac cgcaggatc gaaagtccc tcctctgac 
      agcaccgag ctcgggacc gacgttgca gccttccag 
      agccagagc gagatcgcc agtagcgac gacgaaggc 
      ttcgaagga ttcaaaaaa gacttcatt agcagatta 
      tggctaaca tatcggcgc gagttcccc attctgaac 
      ggctccaac tacagcagc gactgcggc tggggctgc 
      atgctgcgg agcgggcaa atgctgatc gcccaagcc 
      ctagtctgc cacattctg ggcagggac tggcggtgg 
      cagcccgac caccagcct acgacgagg gagagtttc 
      atcgaggtg gtgaaccac cggaaaatt atcaaatgg 
      ttcggcgac aagccctcc aggaacagc cccttttcc 
      atccacacg ctcgtggcc ttgggcgaa gcctcgggc 
      aagaaggcc ggcgactgg tacggcccc gggttcgtc 
      gcgcacctc tttcggcag gcgttcaag cgcgcctct 
      gaggacaac tacgagttc gattcgctg acggtgtgt 
      gtggcccaa gactgcgca gtttacatt aaggatgtg 
      atggaggag tgcacggat aaaaacggc aagtggaaa 
      tctctgatt ttgctgatc ccggtccgg ttaggggcc 
      gagaagttc aactcgatt tacgccccc tgtctcacg 
      accctgttc agcttgaag cagtgcata ggcataatc 
      ggggggcgc cctaaacat tccttgtac ttcgtaggt 
      taccaagac gataagttg attcatttg gacccgcat 
      tactgccag gaagttgtc gatgtttgg gcggttgat 
      tttcccttg acgagtttc cactgtagg tcgccgcgg 
      aaaatccac ttaagcaaa atggatccg tcttgttgt 
      atcggattt tattgccct accaaagag agttttttt 
      aatctcatt gaaattgtc cagcctttg gttattcct 
      cccggacga actgatagc cccggcagc gaatatccc 
      atgttcata ttctgtgat gggtacagc cgtgatgcc 
      cagttgtcc ccgaaaaaa ttacctcca tctgagtta 
      gaatattcc atggatagt agcactgac ggcgatgac 
      atgggcacc gaggcgttt gaaatactg tag
    • >TC014208 RB
      atgaacaca caggatgaa gggaacaat tcggacgtc 
      gaaggcaaa gtcaaaaac agactcctc tcaatgtgg 
      aataacgtg aaatatggg atgaagctc aagacgaac 
      ttctcgaaa gaatctccc gtctggctc ttgggcaag 
      tgctaccgc aggatcgaa agtccctcc tctgacagc 
      accgagctc gggaccgac gttgcagcc ttccagagc 
      cagagcgag atcgccagt agcgacgac gaaggcttc 
      gaaggattc aaaaaagac ttcattagc agattatgg 
      ctaacatat cggcgcgag ttccccatt ctgaacggc 
      tccaactac agcagcgac tgcggctgg ggctgcatg 
      ctgcggagc gggcaaatg ctgatcgcc caagcccta 
      gtctgccac attctgggc agggactgg cggtggcag 
      cccgaccac cagcctacg acgagggag agtttcatc 
      gaggtggtg aaccaccgg aaaattatc aaatggttc 
      ggcgacaag ccctccagg aacagcccc ttttccatc 
      cacacgctc gtggccttg ggcgaagcc tcgggcaag 
      aaggccggc gactggtac ggccccggg ttcgtcgcg 
      cacctcttt cggcaggcg ttcaagcgc gcctctgag 
      gacaactac gagttcgat tcgctgacg gtgtgtgtg 
      gcccaagac tgcgcagtt tacattaag gatgtgatg 
      gaggagtgc acggataaa aacggcaag tggaaatct 
      ctgattttg ctgatcccg gtccggtta ggggccgag 
      aagttcaac tcgatttac gccccctgt ctcacgacc 
      ctgttcagc ttgaagcag tgcataggc ataatcggg 
      gggcgccct aaacattcc ttgtacttc gtaggttac 
      caagacgat aagttgatt catttggac ccgcattac 
      tgccaggaa gttgtcgat gtttgggcg gttgatttt 
      cccttgacg agtttccac tgtaggtcg ccgcggaaa 
      atccactta agcaaaatg gatccgtct tgttgtatc 
      ggattttat tgccctacc aaagagagt ttttttaat 
      ctcattgaa attgtccag cctttggtt attcctccc 
      ggacgaact gatagcccc ggcagcgaa tatcccatg 
      ttcatattc tgtgatggg tacagccgt gatgcccag 
      ttgtccccg aaaaaatta cctccatct gagttagaa 
      tattccatg gatagtagc actgacggc gatgacatg 
      ggcaccgag gcgtttgaa atactgtag
    mRNA sequence
    • >TC014208 RA
      ttgatggcg agccgtggt gttgtttca caaataaat 
      aaaccacaa caaaatgta aatcggaag tttcgagcg 
      aatacgtcg gtggcaagt gaactgtgg aatttgcgt 
      taatcaaga tattgttga ttcttatca ttgataaaa 
      tgaacacac aggtggtat caagagagc gatcaattt 
      ttttgtcca aacttagct tcccaacaa gttcttctg 
      acagtcgtt tgcaggatg aagggaaca attcggacg 
      tcgaaggca aagtcaaaa acagactcc tctcaatgt 
      ggaataacg tgaaatatg ggatgaagc tcaagacga 
      acttctcga aagaatctc ccgtctggc tcttgggca 
      agtgctacc gcaggatcg aaagtccct cctctgaca 
      gcaccgagc tcgggaccg acgttgcag ccttccaga 
      gccagagcg agatcgcca gtagcgacg acgaaggct 
      tcgaaggat tcaaaaaag acttcatta gcagattat 
      ggctaacat atcggcgcg agttcccca ttctgaacg 
      gctccaact acagcagcg actgcggct ggggctgca 
      tgctgcgga gcgggcaaa tgctgatcg cccaagccc 
      tagtctgcc acattctgg gcagggact ggcggtggc 
      agcccgacc accagccta cgacgaggg agagtttca 
      tcgaggtgg tgaaccacc ggaaaatta tcaaatggt 
      tcggcgaca agccctcca ggaacagcc ccttttcca 
      tccacacgc tcgtggcct tgggcgaag cctcgggca 
      agaaggccg gcgactggt acggccccg ggttcgtcg 
      cgcacctct ttcggcagg cgttcaagc gcgcctctg 
      aggacaact acgagttcg attcgctga cggtgtgtg 
      tggcccaag actgcgcag tttacatta aggatgtga 
      tggaggagt gcacggata aaaacggca agtggaaat 
      ctctgattt tgctgatcc cggtccggt taggggccg 
      agaagttca actcgattt acgccccct gtctcacga 
      ccctgttca gcttgaagc agtgcatag gcataatcg 
      gggggcgcc ctaaacatt ccttgtact tcgtaggtt 
      accaagacg ataagttga ttcatttgg acccgcatt 
      actgccagg aagttgtcg atgtttggg cggttgatt 
      ttcccttga cgagtttcc actgtaggt cgccgcgga 
      aaatccact taagcaaaa tggatccgt cttgttgta 
      tcggatttt attgcccta ccaaagaga gttttttta 
      atctcattg aaattgtcc agcctttgg ttattcctc 
      ccggacgaa ctgatagcc ccggcagcg aatatccca 
      tgttcatat tctgtgatg ggtacagcc gtgatgccc 
      agttgtccc cgaaaaaat tacctccat ctgagttag 
      aatattcca tggatagta gcactgacg gcgatgaca 
      tgggcaccg aggcgtttg aaatactgt agttaacca 
      attgttagt tccgtttgt tggttaaaa aggataaat 
      gactgattg ttattaaaa tatcaatat tttaaaatg 
      ttaaagatt ctctattgc tacgactat gtgctatgt 
      cctgaacaa tctatgaca ttttctacc taagttttg 
      tacatatga cttttttat ttgctattt atttaactt 
      atttttgta tttcttagt aaacttgtt actgtgaat 
      gaacgcatt gtttcgttt ctaacaccc tagcctttg 
      ataaaatta tctctttta ccttggcta acagatgtt 
      taatttaat ctcttgttt tagtaaata aaattatca 
      taaacac
    • >TC014208 RB
      ttgatggcg agccgtggt gttgtttca caaataaat 
      aaaccacaa caaaatgta aatcggaag tttcgagcg 
      aatacgtcg gtggcaagt gaactgtgg aatttgcgt 
      taatcaaga tattgttga ttcttatca ttgataaaa 
      tgaacacac aggatgaag ggaacaatt cggacgtcg 
      aaggcaaag tcaaaaaca gactcctct caatgtgga 
      ataacgtga aatatggga tgaagctca agacgaact 
      tctcgaaag aatctcccg tctggctct tgggcaagt 
      gctaccgca ggatcgaaa gtccctcct ctgacagca 
      ccgagctcg ggaccgacg ttgcagcct tccagagcc 
      agagcgaga tcgccagta gcgacgacg aaggcttcg 
      aaggattca aaaaagact tcattagca gattatggc 
      taacatatc ggcgcgagt tccccattc tgaacggct 
      ccaactaca gcagcgact gcggctggg gctgcatgc 
      tgcggagcg ggcaaatgc tgatcgccc aagccctag 
      tctgccaca ttctgggca gggactggc ggtggcagc 
      ccgaccacc agcctacga cgagggaga gtttcatcg 
      aggtggtga accaccgga aaattatca aatggttcg 
      gcgacaagc cctccagga acagcccct tttccatcc 
      acacgctcg tggccttgg gcgaagcct cgggcaaga 
      aggccggcg actggtacg gccccgggt tcgtcgcgc 
      acctctttc ggcaggcgt tcaagcgcg cctctgagg 
      acaactacg agttcgatt cgctgacgg tgtgtgtgg 
      cccaagact gcgcagttt acattaagg atgtgatgg 
      aggagtgca cggataaaa acggcaagt ggaaatctc 
      tgattttgc tgatcccgg tccggttag gggccgaga 
      agttcaact cgatttacg ccccctgtc tcacgaccc 
      tgttcagct tgaagcagt gcataggca taatcgggg 
      ggcgcccta aacattcct tgtacttcg taggttacc 
      aagacgata agttgattc atttggacc cgcattact 
      gccaggaag ttgtcgatg tttgggcgg ttgattttc 
      ccttgacga gtttccact gtaggtcgc cgcggaaaa 
      tccacttaa gcaaaatgg atccgtctt gttgtatcg 
      gattttatt gccctacca aagagagtt tttttaatc 
      tcattgaaa ttgtccagc ctttggtta ttcctcccg 
      gacgaactg atagccccg gcagcgaat atcccatgt 
      tcatattct gtgatgggt acagccgtg atgcccagt 
      tgtccccga aaaaattac ctccatctg agttagaat 
      attccatgg atagtagca ctgacggcg atgacatgg 
      gcaccgagg cgtttgaaa tactgtagt taaccaatt 
      gttagttcc gtttgttgg ttaaaaagg ataaatgac 
      tgattgtta ttaaaatat caatatttt aaaatgtta 
      aagattctc tattgctac gactatgtg ctatgtcct 
      gaacaatct atgacattt tctacctaa gttttgtac 
      atatgactt ttttatttg ctatttatt taacttatt 
      tttgtattt cttagtaaa cttgttact gtgaatgaa 
      cgcattgtt tcgtttcta acaccctag cctttgata 
      aaattatct cttttacct tggctaaca gatgtttaa 
      tttaatctc ttgttttag taaataaaa ttatcataa 
      acac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY