Status
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Gene information
General information
TC014790 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 15114768..15120392
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014790
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10135

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    posterior terminus not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    posterior terminus shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014790 RA
      MANNIRLWN LTQTVQLQT DIKESIKKI SNIDNHLYI 
      QTTSNCLYH GIVQTSENS SSLTLDYCE DWKVIDLDC 
      CKDSLYVVD IDGNVYKCT KNLEKCSEI CLLEDYQCS 
      RGHTGTKCK MKVSKIAVG EYGQLFVTD WGHLWAMGH 
      MPQIGINSD TPRKVNFFN GRTVHSVNV GNDFAIAIV 
      SKQMKSDDT DSDEEDVFV SNCPQCLSA SQLTSPASQ 
      TSFSDLCPL GNKIQGSYD IETTSTSSK NDSSVSSDR 
      RLVHSDDEE EKHDKQKVE KNIIFRNTE AAKEFLTRQ 
      ISRMSSAGE EYLVECTEK PTRIIKENM TNVASFVYE 
      GVKTVGDKV VTLSRHVSG SSDCNSTMD PNPDLTVPR 
      SASKDDGVC SLSQCTSEK DLSEGEIKD RVANIVKSG 
      SELINCEVW TWGNIIHGQ LDCRHLEPN DCLNNPIRQ 
      FLHKFVEAG ADKPTGIGD IIKRERPMI ITKLSNIGV 
      RKVSVQNYH AAVLTLDGR AYIWGRNDH NQVTMDTNM 
      DQSSPKLYN MSKDERVKD VVCGAYYTA VLNHNLNLS 
      YFGKGASKC VDLYCIEGH HEVKEPIVL FRNLLSSMQ 
      YTVLNVGSS GDNFFANFL LEEQKFLEE MLIVQSTVI 
      KPLQKKNIN TANAGLYED LCKSYMDLC YFWAANVQS 
      LLEHCNGLI SLCDIVMFR YLDEFIFVY KNYISTIFN 
      VISIDGFLN ISKLIEISP NLYKQRPDS FTKKEKNNE 
      ENIISTYLL APLDKLETY TTTFDNYSN QLSFVPKWS 
      DFVDEQLHK KQQAEQTRA FWLNSGKVV DQLKSPDRR 
      LIRDSHKYP IHLQNASRF SSHWFVLFS DLFVHMNGS 
      TAHLHNLTT IWIEPQQDE TSQQYQICL KMPEETLVL 
      CTSEPEAKI EWFHALQNA IKAAMNKSE ALQPPVVRN 
      GSYVFRSNG FFKDGTYTG RWSNGKMQG NGKVQWSDG 
      KAYNGQFCN NQLCGYGVM EIPSVGIYE GQWKDNQQN 
      GFGVFTYNN TDVYKGHFK DGQPHGHGF LRKGNFMAN 
      AASFYIGDW VNGMKNGYG VMDDIANGE KYIGNWMDN 
      KKHGNGLII TSDGIYYEG IFNQDVLTG QGVMILEDG 
      TRYEGEFRG TGILYGKGT LTLPSGHTI EGNLSGPWD 
      EGIKISNGT LFRTLEKTP AKTFCSPVH QKWKALFKQ 
      CHQILGVPE KNGAKSLDT QKIWQNVAV YLSNSNSMK 
      RTKNDKKFH NSLNNLDII PPFGREKLD LETYTEVKS 
      YLSKAFESN FHPLGSLLT DLSEAYTTT YGGRAHPLL 
      LGHAIAELH YICKRLYEI VRFLFPALP PYDKESLVE 
      NEDSEEIVN YQSLLYPLI LPKVHHPLF TLFTLKNEQ 
      QERQYKRTL MEWNKQSDT ALMTFLSVD QKFFHSEDQ 
      QIAHIFEDA IETLQQLKT TFSPTEKLL VIRQTVEKM 
      TPVAQELLG VNYVWNMDD LFPIFLFVV VRARIPDLG 
      SELDFVDNF MDPSLESGE LGIMYTTLK ASYQQILQE 
      KVSSY
    CDS sequence
    • >TC014790 RA
      atggccaat aacatacgt ttatggaac ctgacccag 
      accgtgcaa ctccagact gacatcaaa gaaagcata 
      aagaaaata agcaatatc gataaccac ctgtatatc 
      caaacaacc agcaactgt ttataccac ggaatcgtc 
      caaacaagc gaaaattcc tcgtccctt accttggat 
      tattgcgaa gattggaaa gtcatagat ttagattgc 
      tgtaaggat agtttatat gtggtagac atagacgga 
      aacgtgtat aaatgtaca aaaaacctg gaaaagtgt 
      agcgaaatt tgcctcctt gaagactac cagtgttca 
      agaggccac accggcacc aagtgcaaa atgaaagtg 
      agcaaaatc gcagttggg gaatacgga caattgttt 
      gtaactgac tggggccac ttgtgggca atggggcac 
      atgccccag atcggaatc aactcagac acccccaga 
      aaagtgaac tttttcaac gggcggacg gtacacagc 
      gtcaatgtc ggtaacgac tttgctata gccatagtc 
      agcaaacaa atgaagagc gatgatacc gacagtgat 
      gaagaggat gtttttgtt tcaaactgc ccccagtgt 
      ttatcagcc tcacaacta acctcgcct gcgtcacaa 
      acctccttt tcggacttg tgccccctt ggcaacaaa 
      atccaagga agttacgac attgaaacc acgtccaca 
      agtagcaaa aacgacagc agcgtaagc tccgaccgt 
      cgactcgtc cacagcgac gacgaggag gaaaaacac 
      gataagcaa aaagtcgaa aaaaacata atttttcgc 
      aacactgaa gcagccaaa gaattccta actcggcaa 
      atttcgcgc atgtcttcg gccggtgag gaatacctt 
      gtcgaatgc accgaaaaa cccacccga ataatcaaa 
      gaaaatatg accaacgtt gccagtttc gtctacgaa 
      ggggtgaaa acagttggt gataaagtg gttacgtta 
      tcgcgacat gtgagcgga agctcggat tgtaacagc 
      accatggac cctaatccc gacttgact gttccacgg 
      tcagcctcc aaagacgat ggggtttgt tcgctgagt 
      caatgcacg tctgagaaa gacctcagt gagggtgaa 
      attaaggat cgtgtggcg aatattgtg aaaagtgga 
      tcagaactt atcaactgc gaggtgtgg acgtggggg 
      aatatcatt cacggccag ttagattgc cgacatttg 
      gagccgaat gattgctta aacaatccg attcgacag 
      tttttgcat aaatttgtc gaggcgggt gcggacaag 
      ccaacagga atcggcgac ataatcaaa cgcgaacgc 
      cccatgatc atcaccaag ttatcaaat atcggcgtc 
      cgcaaagtg tccgttcaa aattaccac gctgctgtt 
      ttaacacta gacgggaga gcgtacatt tggggccga 
      aacgaccac aatcaagtc acaatggac actaacatg 
      gaccagagt tcaccaaaa ttgtacaac atgagtaaa 
      gacgagcgc gttaaagac gtagtctgt ggtgcttat 
      tatacggcc gttttaaac cacaattta aatttgagt 
      tatttcgga aagggggct agcaaatgt gtggattta 
      tactgcatt gagggccat catgaagtc aaggaacca 
      atcgtgttg tttagaaat ttattatcg agcatgcaa 
      tacaccgtt ttgaatgtt ggctcaagt ggggataat 
      tttttcgcc aattttcta ctagaagag cagaagttt 
      ctggaggag atgttgata gtacaaagt actgtgatt 
      aagccacta cagaagaaa aatattaac acggctaat 
      gcgggcctg tatgaggat ttgtgtaag agttacatg 
      gacttgtgt tatttttgg gccgccaat gtacagtcg 
      ttgctcgag cattgcaac ggtttgatc tcactttgt 
      gatattgtc atgttccga tatttggac gagtttatc 
      ttcgtctac aagaattac atcagtaca atatttaac 
      gttataagt atcgacggt tttctaaac atatcaaaa 
      ttaatcgaa atatcacca aacttgtac aagcagcga 
      cctgatagt ttcaccaaa aaggagaaa aacaatgag 
      gaaaatatc atttcgact tacctcctg gcccccctt 
      gacaaactc gagacttac acgaccacc ttcgataat 
      tattcgaac caattgtca tttgtcccg aaatggtcc 
      gactttgtg gacgaacag ttgcacaaa aaacagcaa 
      gctgagcaa acccgagcc ttttggttg aattcgggt 
      aaagtcgtc gaccaattg aaatcaccc gaccgtcgt 
      ttgatccgt gatagtcac aagtacccg atccacctg 
      caaaatgcc agtcggttt agttcgcat tggttcgtc 
      ctgttttcg gatcttttc gttcacatg aacggctcc 
      acagcccac ttgcacaac ctcaccacc atttggatt 
      gagcctcag caggacgaa acctcccaa cagtaccag 
      atctgtctc aaaatgccc gaagagacg ctcgttttg 
      tgcacgagt gaacccgag gcgaagatc gagtggttt 
      cacgcgtta cagaacgct atcaaggcg gcgatgaat 
      aaaagtgag gcgttgcaa ccgccagtg gtgcggaat 
      gggagttac gttttcagg agtaatggg ttttttaag 
      gatgggacg tatacggga cgatggtcc aatgggaag 
      atgcaaggg aatgggaag gtgcaatgg agcgatggg 
      aaggcgtat aatgggcag ttctgcaac aaccagttg 
      tgtgggtat ggggtcatg gagatacct agtgttggg 
      atatatgaa ggccagtgg aaagacaac cagcagaac 
      ggcttcggc gtcttcact tacaacaac acggacgtc 
      tacaagggc catttcaag gatgggcaa ccccatggg 
      catgggttc ctccgtaag ggtaatttc atggccaat 
      gctgcgtcc ttttacatc ggcgattgg gtaaatggc 
      atgaagaac ggttacggc gtcatggac gacatcgcc 
      aacggcgaa aaatacatt ggtaattgg atggacaac 
      aaaaagcac ggcaatggg ctaattatc acctctgat 
      ggtatttac tacgaaggg attttcaac caggacgtc 
      ctaacaggc caaggcgtg atgatcctc gaagatggt 
      acacggtac gaaggcgag ttccggggc accggaatt 
      ttgtatgga aaaggcacc ctcacgtta ccctccgga 
      cataccatt gagggtaac ttgagcggg ccatgggac 
      gaaggtata aaaatttcc aatgggacg cttttccga 
      acgttggaa aaaacccca gcaaagaca ttctgttct 
      cccgtccat caaaaatgg aaagctttg ttcaaacaa 
      tgccaccag attttggga gtccccgaa aaaaacggc 
      gcaaagtca cttgacacc caaaaaatt tggcaaaac 
      gtcgctgtg tatttatca aacagcaat tcgatgaaa 
      cgaacaaaa aatgataaa aagttccac aattccttg 
      aataatctt gatataatc ccgccgttt gggcgcgaa 
      aaactcgac ctggagacc tacaccgaa gtcaagagt 
      tatttatcg aaggcattt gaaagcaat ttccacccg 
      ttagggtcc ctccttacc gacttatcc gaagcgtac 
      accacgacc tacggtgga cgggcccac cctctcctc 
      ctcggccac gcaatagcc gagttacac tacatttgt 
      aaaagactg tacgaaatc gtccgattc ctattccca 
      gcccttccg ccctacgac aaagaatcc cttgttgaa 
      aacgaagat tccgaagaa atagtcaat taccagtcc 
      ctactttac ccacttatt ttgcccaaa gtccaccac 
      ccgcttttc actctcttc accttgaaa aacgagcaa 
      caggaacga cagtacaaa cgcactctt atggaatgg 
      aacaaacaa tcggacacc gcattaatg acatttctc 
      tcagtggac cagaaattt tttcattcg gaagaccaa 
      caaatcgca catattttc gaagacgcg attgagact 
      ttgcaacaa ttgaaaacg accttttcg ccgactgaa 
      aaattgcta gtgatacgg cagacagtt gagaagatg 
      acaccagtg gcccaggaa ctgttgggt gtgaattat 
      gtgtggaat atggacgac ctttttccg attttttta 
      tttgtggtg gtgagggcg agaataccc gatttgggc 
      tcggagctc gattttgtc gataatttt atggacccg 
      agtctggag agcggcgaa ttgggcatt atgtacaca 
      acactaaaa gccagttac cagcaaata ctgcaggag 
      aaagtatcc agttattaa
    mRNA sequence
    • >TC014790 RA
      gctaaatgc tctctttta tatttttcg ttgaatttg 
      ttgtcctgt cattttgac aatcagctg atcggtatg 
      ggaaggtgt ggtttgaat tatttagtg ttccggtgc 
      tccgtttaa tttccttcc tccatccat ccaaaattt 
      tagtacaac ttaagacaa tggccaata acatacgtt 
      tatggaacc tgacccaga ccgtgcaac tccagactg 
      acatcaaag aaagcataa agaaaataa gcaatatcg 
      ataaccacc tgtatatcc aaacaacca gcaactgtt 
      tataccacg gaatcgtcc aaacaagcg aaaattcct 
      cgtccctta ccttggatt attgcgaag attggaaag 
      tcatagatt tagattgct gtaaggata gtttatatg 
      tggtagaca tagacggaa acgtgtata aatgtacaa 
      aaaacctgg aaaagtgta gcgaaattt gcctccttg 
      aagactacc agtgttcaa gaggccaca ccggcacca 
      agtgcaaaa tgaaagtga gcaaaatcg cagttgggg 
      aatacggac aattgtttg taactgact ggggccact 
      tgtgggcaa tggggcaca tgccccaga tcggaatca 
      actcagaca cccccagaa aagtgaact ttttcaacg 
      ggcggacgg tacacagcg tcaatgtcg gtaacgact 
      ttgctatag ccatagtca gcaaacaaa tgaagagcg 
      atgataccg acagtgatg aagaggatg tttttgttt 
      caaactgcc cccagtgtt tatcagcct cacaactaa 
      cctcgcctg cgtcacaaa cctcctttt cggacttgt 
      gcccccttg gcaacaaaa tccaaggaa gttacgaca 
      ttgaaacca cgtccacaa gtagcaaaa acgacagca 
      gcgtaagct ccgaccgtc gactcgtcc acagcgacg 
      acgaggagg aaaaacacg ataagcaaa aagtcgaaa 
      aaaacataa tttttcgca acactgaag cagccaaag 
      aattcctaa ctcggcaaa tttcgcgca tgtcttcgg 
      ccggtgagg aataccttg tcgaatgca ccgaaaaac 
      ccacccgaa taatcaaag aaaatatga ccaacgttg 
      ccagtttcg tctacgaag gggtgaaaa cagttggtg 
      ataaagtgg ttacgttat cgcgacatg tgagcggaa 
      gctcggatt gtaacagca ccatggacc ctaatcccg 
      acttgactg ttccacggt cagcctcca aagacgatg 
      gggtttgtt cgctgagtc aatgcacgt ctgagaaag 
      acctcagtg agggtgaaa ttaaggatc gtgtggcga 
      atattgtga aaagtggat cagaactta tcaactgcg 
      aggtgtgga cgtggggga atatcattc acggccagt 
      tagattgcc gacatttgg agccgaatg attgcttaa 
      acaatccga ttcgacagt ttttgcata aatttgtcg 
      aggcgggtg cggacaagc caacaggaa tcggcgaca 
      taatcaaac gcgaacgcc ccatgatca tcaccaagt 
      tatcaaata tcggcgtcc gcaaagtgt ccgttcaaa 
      attaccacg ctgctgttt taacactag acgggagag 
      cgtacattt ggggccgaa acgaccaca atcaagtca 
      caatggaca ctaacatgg accagagtt caccaaaat 
      tgtacaaca tgagtaaag acgagcgcg ttaaagacg 
      tagtctgtg gtgcttatt atacggccg ttttaaacc 
      acaatttaa atttgagtt atttcggaa agggggcta 
      gcaaatgtg tggatttat actgcattg agggccatc 
      atgaagtca aggaaccaa tcgtgttgt ttagaaatt 
      tattatcga gcatgcaat acaccgttt tgaatgttg 
      gctcaagtg gggataatt ttttcgcca attttctac 
      tagaagagc agaagtttc tggaggaga tgttgatag 
      tacaaagta ctgtgatta agccactac agaagaaaa 
      atattaaca cggctaatg cgggcctgt atgaggatt 
      tgtgtaaga gttacatgg acttgtgtt atttttggg 
      ccgccaatg tacagtcgt tgctcgagc attgcaacg 
      gtttgatct cactttgtg atattgtca tgttccgat 
      atttggacg agtttatct tcgtctaca agaattaca 
      tcagtacaa tatttaacg ttataagta tcgacggtt 
      ttctaaaca tatcaaaat taatcgaaa tatcaccaa 
      acttgtaca agcagcgac ctgatagtt tcaccaaaa 
      aggagaaaa acaatgagg aaaatatca tttcgactt 
      acctcctgg ccccccttg acaaactcg agacttaca 
      cgaccacct tcgataatt attcgaacc aattgtcat 
      ttgtcccga aatggtccg actttgtgg acgaacagt 
      tgcacaaaa aacagcaag ctgagcaaa cccgagcct 
      tttggttga attcgggta aagtcgtcg accaattga 
      aatcacccg accgtcgtt tgatccgtg atagtcaca 
      agtacccga tccacctgc aaaatgcca gtcggttta 
      gttcgcatt ggttcgtcc tgttttcgg atcttttcg 
      ttcacatga acggctcca cagcccact tgcacaacc 
      tcaccacca tttggattg agcctcagc aggacgaaa 
      cctcccaac agtaccaga tctgtctca aaatgcccg 
      aagagacgc tcgttttgt gcacgagtg aacccgagg 
      cgaagatcg agtggtttc acgcgttac agaacgcta 
      tcaaggcgg cgatgaata aaagtgagg cgttgcaac 
      cgccagtgg tgcggaatg ggagttacg ttttcagga 
      gtaatgggt tttttaagg atgggacgt atacgggac 
      gatggtcca atgggaaga tgcaaggga atgggaagg 
      tgcaatgga gcgatggga aggcgtata atgggcagt 
      tctgcaaca accagttgt gtgggtatg gggtcatgg 
      agataccta gtgttggga tatatgaag gccagtgga 
      aagacaacc agcagaacg gcttcggcg tcttcactt 
      acaacaaca cggacgtct acaagggcc atttcaagg 
      atgggcaac cccatgggc atgggttcc tccgtaagg 
      gtaatttca tggccaatg ctgcgtcct tttacatcg 
      gcgattggg taaatggca tgaagaacg gttacggcg 
      tcatggacg acatcgcca acggcgaaa aatacattg 
      gtaattgga tggacaaca aaaagcacg gcaatgggc 
      taattatca cctctgatg gtatttact acgaaggga 
      ttttcaacc aggacgtcc taacaggcc aaggcgtga 
      tgatcctcg aagatggta cacggtacg aaggcgagt 
      tccggggca ccggaattt tgtatggaa aaggcaccc 
      tcacgttac cctccggac ataccattg agggtaact 
      tgagcgggc catgggacg aaggtataa aaatttcca 
      atgggacgc ttttccgaa cgttggaaa aaaccccag 
      caaagacat tctgttctc ccgtccatc aaaaatgga 
      aagctttgt tcaaacaat gccaccaga ttttgggag 
      tccccgaaa aaaacggcg caaagtcac ttgacaccc 
      aaaaaattt ggcaaaacg tcgctgtgt atttatcaa 
      acagcaatt cgatgaaac gaacaaaaa atgataaaa 
      agttccaca attccttga ataatcttg atataatcc 
      cgccgtttg ggcgcgaaa aactcgacc tggagacct 
      acaccgaag tcaagagtt atttatcga aggcatttg 
      aaagcaatt tccacccgt tagggtccc tccttaccg 
      acttatccg aagcgtaca ccacgacct acggtggac 
      gggcccacc ctctcctcc tcggccacg caatagccg 
      agttacact acatttgta aaagactgt acgaaatcg 
      tccgattcc tattcccag cccttccgc cctacgaca 
      aagaatccc ttgttgaaa acgaagatt ccgaagaaa 
      tagtcaatt accagtccc tactttacc cacttattt 
      tgcccaaag tccaccacc cgcttttca ctctcttca 
      ccttgaaaa acgagcaac aggaacgac agtacaaac 
      gcactctta tggaatgga acaaacaat cggacaccg 
      cattaatga catttctct cagtggacc agaaatttt 
      ttcattcgg aagaccaac aaatcgcac atattttcg 
      aagacgcga ttgagactt tgcaacaat tgaaaacga 
      ccttttcgc cgactgaaa aattgctag tgatacggc 
      agacagttg agaagatga caccagtgg cccaggaac 
      tgttgggtg tgaattatg tgtggaata tggacgacc 
      tttttccga tttttttat ttgtggtgg tgagggcga 
      gaatacccg atttgggct cggagctcg attttgtcg 
      ataatttta tggacccga gtctggaga gcggcgaat 
      tgggcatta tgtacacaa cactaaaag ccagttacc 
      agcaaatac tgcaggaga aagtatcca gttattaaa 
      tcgtagatt ttatttttt taataaaga gagaacgtt 
      ttgtgatac gataattct tgttattat ttttatata 
      ttttatttt agggtttat taaagtgca aacatatca 
      tgtatgtga aataacttt ttttgtacc tgattttgt 
      ctcagtaac tgacaatcg tggcgagat aatagtaat 
      aaataatta tgaaacgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY