Status
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Gene information
General information
TC014774 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14892527..14895950
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014774
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10133

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    abdominal odoriferous glands potentially content not present - Number of animals showing the phenotype: 6 (13 dpi)
    Days post injection



    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection



    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature symmetry - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs size shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs femur distal fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs tibiotarsus shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014774 RA
      MSEQGQLSN NSANSEEGD AAGVPGGKP QAPSQTQNA 
      APTNKQPAS RQTNLQRIQ QKKQAVLAF PYNKKLLKL 
      AIYSKCQSE NCICTGWKK TDPNMQEPT FTDPCRCEH 
      PLEMHISHL RSKTEDELN RLLRMVVDI DNMGTAMSR 
      EENIETKKV YTYLFHLLR KCILTLDTP VVESSLGQP 
      PFEKPCIHK AITNLLVYK FSHLAQQEW KTMYEVARI 
      LLHCLNTWE FPSPSSQKH IVSPEEATV YKMEHMRWL 
      VFCNVPTFC DSLKHYDTT MVFGRTLLR AVFKYMRKQ 
      IMDQFNRER DKMPQERRV MLLTHFPPF LNTLDEEIY 
      AQDSPIWDP DFKQGPPQH FQNLFEAGK NKTNAKRGA 
      SDLDAKAGK TGDVPSRET KRRRAQDEQ FEDIPMETV 
      AQIIATIDD PNYMTGPDL IFSENAPAT DEAPKLLEK 
      RKVIEMHVI GNSLTETVS KQTMLWFIG LQNVFSLQL 
      PRMPIEYIT QLLFDPKHR TIALIKENR PIGGICFRP 
      FETQGFTEI VFCAVTFSE QIKGYGTHL MNHLKDYHI 
      RKGILHFLT FADENAIGY FERQGFSKD IKLNRSIYQ 
      GYIKDYEGA TLMHCELNP KIIYTEFTS VVRRQKKFV 
      KQLIYQQQR NVSKVHPGL TFFKEGVKS IPIESIPGL 
      QETGWKPAA RATRGAQQL EESQDIDVL ADMLKSVLQ 
      AVKSHEDSW PFRLPVDKN DVPDYYDHI KYPMDLKTM 
      ADRLKSRYY VSRRLFIAD MMRIFRNCK IYNSPETEY 
      YQCAVNLQQ YFQTKMKEV GLWDK
    CDS sequence
    • >TC014774 RA
      atgtcagaa cagggccag ttgagtaac aatagtgcc 
      aattctgaa gaaggggat gccgcagga gtccctggg 
      ggtaaaccc caggctccc agtcagaca caaaatgca 
      gccccaact aataaacaa ccagcgtcg agacagacg 
      aatttgcaa agaatacaa cagaagaaa caggccgtt 
      ttggccttc ccgtacaat aaaaagttg ctgaaactt 
      gccatttat tcaaaatgc cagagcgag aactgcatt 
      tgcaccggt tggaaaaag accgacccc aacatgcaa 
      gagcccact tttaccgac ccctgtcgt tgcgagcac 
      cctttagaa atgcacatt tcccactta cgcagtaaa 
      actgaggac gagttaaat cgtctcctg cgtatggtt 
      gtggacatt gacaatatg ggtacggca atgagtcgt 
      gaagaaaac attgaaaca aaaaaagtt tacacgtac 
      ttattccac ttgttgcgt aaatgcatc ctaactctt 
      gacactccc gtcgttgag agttcgctc ggacagccc 
      ccatttgaa aaaccgtgc attcacaaa gcgatcaca 
      aatttgcta gtttacaaa ttctcacat ttggcccaa 
      caagagtgg aagacgatg tatgaagtg gctaggatt 
      ctcctccac tgtttgaac acgtgggag tttccgagt 
      ccgagcagc cagaagcat attgtaagt ccggaggaa 
      gccacagtt tataaaatg gaacatatg cgttggttg 
      gtgttttgc aacgttcca acattttgc gactcgttg 
      aaacattac gatacgaca atggtgttt gggaggacg 
      cttctaagg gctgttttc aaatacatg aggaagcaa 
      atcatggat cagtttaat cgagagcgg gacaaaatg 
      ccccaagaa cgtcgcgtt atgctcctc acacatttt 
      cccccgttt ctgaacacg ttggatgaa gagatttac 
      gcgcaggat tccccgatt tgggatcct gattttaaa 
      caaggaccg ccgcaacat ttccagaat ttattcgaa 
      gcggggaaa aacaaaacg aatgccaag cgaggagcg 
      agcgatttg gacgcgaaa gcgggcaaa actggggat 
      gtaccgagt cgagagacg aaacgtagg agggcacag 
      gatgaacag tttgaggat attccgatg gagacggtt 
      gcacaaatt attgcaaca attgatgat ccgaattac 
      atgaccgga cctgatctg attttctcc gagaatgcg 
      cccgccaca gacgaagcg cccaaactt ttagaaaaa 
      cgcaaagtg attgaaatg cacgtcatt ggtaacagt 
      ttgaccgaa acggtctcg aaacagacc atgttgtgg 
      ttcattggt ctacaaaac gtcttttcg cttcaattg 
      ccccgaatg ccgatcgaa tacatcacc caattgtta 
      ttcgatcca aaacaccgc accattgcc ctaattaaa 
      gaaaaccgt ccaatcggc ggtatttgt ttccgcccg 
      tttgaaact caaggtttc acagagatt gttttttgc 
      gcggtcact ttcagcgaa cagatcaaa ggttacgga 
      acgcattta atgaaccat ttgaaagac taccacatt 
      cgtaaaggc attctccac tttctgact ttcgcagac 
      gagaacgcg attggttat ttcgaacgt cagggattt 
      tcgaaagat attaaatta aaccggtcg atttatcaa 
      ggctatatt aaggactat gaaggcgcc actctcatg 
      cattgcgaa ctgaacccc aaaataatc tacaccgag 
      ttcacttca gtcgttcgg aggcaaaaa aagttcgtc 
      aaacagttg atttatcag cagcaaagg aacgtatca 
      aaggttcac cccggtctg actttcttc aaagagggg 
      gtgaagagc atccccatt gagtccatt ccggggctg 
      caggagaca ggctggaaa ccggcagcg agggccacc 
      agaggggcg caacaactg gaggagtct caagatatt 
      gacgtgttg gctgatatg ctcaaaagt gtgcttcaa 
      gcggtgaaa agtcacgaa gattcttgg ccgtttcga 
      ctgccagtt gataaaaac gacgttccg gattattac 
      gaccacatc aagtatccc atggatttg aaaacaatg 
      gccgatcgc ttgaagtca cggtattac gtttcaagg 
      cgtttgttt atcgctgat atgatgcgg attttccgc 
      aattgtaaa atttataat tcgcctgag acagagtat 
      taccagtgt gctgtgaac ttgcagcag tatttccag 
      accaagatg aaggaagtc ggtttgtgg gacaaataa
    mRNA sequence
    • >TC014774 RA
      ttttacaaa aaaatacaa ttaaaacct tattaaatt 
      tgtttatta gacatgatt cgaccgtat taattactt 
      acaacagta agtagcaaa gaagttaag ttattgttt 
      tataaacaa aaatgtcag aacagggcc agttgagta 
      acaatagtg ccaattctg aagaagggg atgccgcag 
      gagtccctg ggggtaaac cccaggctc ccagtcaga 
      cacaaaatg cagccccaa ctaataaac aaccagcgt 
      cgagacaga cgaatttgc aaagaatac aacagaaga 
      aacaggccg ttttggcct tcccgtaca ataaaaagt 
      tgctgaaac ttgccattt attcaaaat gccagagcg 
      agaactgca tttgcaccg gttggaaaa agaccgacc 
      ccaacatgc aagagccca cttttaccg acccctgtc 
      gttgcgagc accctttag aaatgcaca tttcccact 
      tacgcagta aaactgagg acgagttaa atcgtctcc 
      tgcgtatgg ttgtggaca ttgacaata tgggtacgg 
      caatgagtc gtgaagaaa acattgaaa caaaaaaag 
      tttacacgt acttattcc acttgttgc gtaaatgca 
      tcctaactc ttgacactc ccgtcgttg agagttcgc 
      tcggacagc ccccatttg aaaaaccgt gcattcaca 
      aagcgatca caaatttgc tagtttaca aattctcac 
      atttggccc aacaagagt ggaagacga tgtatgaag 
      tggctagga ttctcctcc actgtttga acacgtggg 
      agtttccga gtccgagca gccagaagc atattgtaa 
      gtccggagg aagccacag tttataaaa tggaacata 
      tgcgttggt tggtgtttt gcaacgttc caacatttt 
      gcgactcgt tgaaacatt acgatacga caatggtgt 
      ttgggagga cgcttctaa gggctgttt tcaaataca 
      tgaggaagc aaatcatgg atcagttta atcgagagc 
      gggacaaaa tgccccaag aacgtcgcg ttatgctcc 
      tcacacatt ttcccccgt ttctgaaca cgttggatg 
      aagagattt acgcgcagg attccccga tttgggatc 
      ctgatttta aacaaggac cgccgcaac atttccaga 
      atttattcg aagcgggga aaaacaaaa cgaatgcca 
      agcgaggag cgagcgatt tggacgcga aagcgggca 
      aaactgggg atgtaccga gtcgagaga cgaaacgta 
      ggagggcac aggatgaac agtttgagg atattccga 
      tggagacgg ttgcacaaa ttattgcaa caattgatg 
      atccgaatt acatgaccg gacctgatc tgattttct 
      ccgagaatg cgcccgcca cagacgaag cgcccaaac 
      ttttagaaa aacgcaaag tgattgaaa tgcacgtca 
      ttggtaaca gtttgaccg aaacggtct cgaaacaga 
      ccatgttgt ggttcattg gtctacaaa acgtctttt 
      cgcttcaat tgccccgaa tgccgatcg aatacatca 
      cccaattgt tattcgatc caaaacacc gcaccattg 
      ccctaatta aagaaaacc gtccaatcg gcggtattt 
      gtttccgcc cgtttgaaa ctcaaggtt tcacagaga 
      ttgtttttt gcgcggtca ctttcagcg aacagatca 
      aaggttacg gaacgcatt taatgaacc atttgaaag 
      actaccaca ttcgtaaag gcattctcc actttctga 
      ctttcgcag acgagaacg cgattggtt atttcgaac 
      gtcagggat tttcgaaag atattaaat taaaccggt 
      cgatttatc aaggctata ttaaggact atgaaggcg 
      ccactctca tgcattgcg aactgaacc ccaaaataa 
      tctacaccg agttcactt cagtcgttc ggaggcaaa 
      aaaagttcg tcaaacagt tgatttatc agcagcaaa 
      ggaacgtat caaaggttc accccggtc tgactttct 
      tcaaagagg gggtgaaga gcatcccca ttgagtcca 
      ttccggggc tgcaggaga caggctgga aaccggcag 
      cgagggcca ccagagggg cgcaacaac tggaggagt 
      ctcaagata ttgacgtgt tggctgata tgctcaaaa 
      gtgtgcttc aagcggtga aaagtcacg aagattctt 
      ggccgtttc gactgccag ttgataaaa acgacgttc 
      cggattatt acgaccaca tcaagtatc ccatggatt 
      tgaaaacaa tggccgatc gcttgaagt cacggtatt 
      acgtttcaa ggcgtttgt ttatcgctg atatgatgc 
      ggattttcc gcaattgta aaatttata attcgcctg 
      agacagagt attaccagt gtgctgtga acttgcagc 
      agtatttcc agaccaaga tgaaggaag tcggtttgt 
      gggacaaat aatttaaga gaaaaccaa tatatttag 
      tgttttata cattacata ataaaacga gtatgaatt 
      ataataata ataataaaa atgactagt aaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY