Status
Please wait ...
Gene information
General information
TC014774 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14892527..14895950
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC014774
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10133

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Adult morphology
    abdominal odoriferous glands potentially content not present - Number of animals showing the phenotype: 6 (13 dpi)
    Days post injection


    abdominal odoriferous glands content decreased - Number of animals showing the phenotype: 2 (13 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature symmetry - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs size shortened - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs femur distal fused - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    legs tibiotarsus shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014774 RA
      MSEQGQLSN NSANSEEGD AAGVPGGKP QAPSQTQNA 
APTNKQPAS RQTNLQRIQ QKKQAVLAF PYNKKLLKL 
AIYSKCQSE NCICTGWKK TDPNMQEPT FTDPCRCEH 
PLEMHISHL RSKTEDELN RLLRMVVDI DNMGTAMSR 
EENIETKKV YTYLFHLLR KCILTLDTP VVESSLGQP 
PFEKPCIHK AITNLLVYK FSHLAQQEW KTMYEVARI 
LLHCLNTWE FPSPSSQKH IVSPEEATV YKMEHMRWL 
VFCNVPTFC DSLKHYDTT MVFGRTLLR AVFKYMRKQ 
IMDQFNRER DKMPQERRV MLLTHFPPF LNTLDEEIY 
AQDSPIWDP DFKQGPPQH FQNLFEAGK NKTNAKRGA 
SDLDAKAGK TGDVPSRET KRRRAQDEQ FEDIPMETV 
AQIIATIDD PNYMTGPDL IFSENAPAT DEAPKLLEK 
RKVIEMHVI GNSLTETVS KQTMLWFIG LQNVFSLQL 
PRMPIEYIT QLLFDPKHR TIALIKENR PIGGICFRP 
FETQGFTEI VFCAVTFSE QIKGYGTHL MNHLKDYHI 
RKGILHFLT FADENAIGY FERQGFSKD IKLNRSIYQ 
GYIKDYEGA TLMHCELNP KIIYTEFTS VVRRQKKFV 
KQLIYQQQR NVSKVHPGL TFFKEGVKS IPIESIPGL 
QETGWKPAA RATRGAQQL EESQDIDVL ADMLKSVLQ 
AVKSHEDSW PFRLPVDKN DVPDYYDHI KYPMDLKTM 
ADRLKSRYY VSRRLFIAD MMRIFRNCK IYNSPETEY 
YQCAVNLQQ YFQTKMKEV GLWDK
    CDS sequence
    • >TC014774 RA
      atgtcagaa cagggccag ttgagtaac aatagtgcc 
aattctgaa gaaggggat gccgcagga gtccctggg 
ggtaaaccc caggctccc agtcagaca caaaatgca 
gccccaact aataaacaa ccagcgtcg agacagacg 
aatttgcaa agaatacaa cagaagaaa caggccgtt 
ttggccttc ccgtacaat aaaaagttg ctgaaactt 
gccatttat tcaaaatgc cagagcgag aactgcatt 
tgcaccggt tggaaaaag accgacccc aacatgcaa 
gagcccact tttaccgac ccctgtcgt tgcgagcac 
cctttagaa atgcacatt tcccactta cgcagtaaa 
actgaggac gagttaaat cgtctcctg cgtatggtt 
gtggacatt gacaatatg ggtacggca atgagtcgt 
gaagaaaac attgaaaca aaaaaagtt tacacgtac 
ttattccac ttgttgcgt aaatgcatc ctaactctt 
gacactccc gtcgttgag agttcgctc ggacagccc 
ccatttgaa aaaccgtgc attcacaaa gcgatcaca 
aatttgcta gtttacaaa ttctcacat ttggcccaa 
caagagtgg aagacgatg tatgaagtg gctaggatt 
ctcctccac tgtttgaac acgtgggag tttccgagt 
ccgagcagc cagaagcat attgtaagt ccggaggaa 
gccacagtt tataaaatg gaacatatg cgttggttg 
gtgttttgc aacgttcca acattttgc gactcgttg 
aaacattac gatacgaca atggtgttt gggaggacg 
cttctaagg gctgttttc aaatacatg aggaagcaa 
atcatggat cagtttaat cgagagcgg gacaaaatg 
ccccaagaa cgtcgcgtt atgctcctc acacatttt 
cccccgttt ctgaacacg ttggatgaa gagatttac 
gcgcaggat tccccgatt tgggatcct gattttaaa 
caaggaccg ccgcaacat ttccagaat ttattcgaa 
gcggggaaa aacaaaacg aatgccaag cgaggagcg 
agcgatttg gacgcgaaa gcgggcaaa actggggat 
gtaccgagt cgagagacg aaacgtagg agggcacag 
gatgaacag tttgaggat attccgatg gagacggtt 
gcacaaatt attgcaaca attgatgat ccgaattac 
atgaccgga cctgatctg attttctcc gagaatgcg 
cccgccaca gacgaagcg cccaaactt ttagaaaaa 
cgcaaagtg attgaaatg cacgtcatt ggtaacagt 
ttgaccgaa acggtctcg aaacagacc atgttgtgg 
ttcattggt ctacaaaac gtcttttcg cttcaattg 
ccccgaatg ccgatcgaa tacatcacc caattgtta 
ttcgatcca aaacaccgc accattgcc ctaattaaa 
gaaaaccgt ccaatcggc ggtatttgt ttccgcccg 
tttgaaact caaggtttc acagagatt gttttttgc 
gcggtcact ttcagcgaa cagatcaaa ggttacgga 
acgcattta atgaaccat ttgaaagac taccacatt 
cgtaaaggc attctccac tttctgact ttcgcagac 
gagaacgcg attggttat ttcgaacgt cagggattt 
tcgaaagat attaaatta aaccggtcg atttatcaa 
ggctatatt aaggactat gaaggcgcc actctcatg 
cattgcgaa ctgaacccc aaaataatc tacaccgag 
ttcacttca gtcgttcgg aggcaaaaa aagttcgtc 
aaacagttg atttatcag cagcaaagg aacgtatca 
aaggttcac cccggtctg actttcttc aaagagggg 
gtgaagagc atccccatt gagtccatt ccggggctg 
caggagaca ggctggaaa ccggcagcg agggccacc 
agaggggcg caacaactg gaggagtct caagatatt 
gacgtgttg gctgatatg ctcaaaagt gtgcttcaa 
gcggtgaaa agtcacgaa gattcttgg ccgtttcga 
ctgccagtt gataaaaac gacgttccg gattattac 
gaccacatc aagtatccc atggatttg aaaacaatg 
gccgatcgc ttgaagtca cggtattac gtttcaagg 
cgtttgttt atcgctgat atgatgcgg attttccgc 
aattgtaaa atttataat tcgcctgag acagagtat 
taccagtgt gctgtgaac ttgcagcag tatttccag 
accaagatg aaggaagtc ggtttgtgg gacaaataa
    mRNA sequence
    • >TC014774 RA
      ttttacaaa aaaatacaa ttaaaacct tattaaatt 
tgtttatta gacatgatt cgaccgtat taattactt 
acaacagta agtagcaaa gaagttaag ttattgttt 
tataaacaa aaatgtcag aacagggcc agttgagta 
acaatagtg ccaattctg aagaagggg atgccgcag 
gagtccctg ggggtaaac cccaggctc ccagtcaga 
cacaaaatg cagccccaa ctaataaac aaccagcgt 
cgagacaga cgaatttgc aaagaatac aacagaaga 
aacaggccg ttttggcct tcccgtaca ataaaaagt 
tgctgaaac ttgccattt attcaaaat gccagagcg 
agaactgca tttgcaccg gttggaaaa agaccgacc 
ccaacatgc aagagccca cttttaccg acccctgtc 
gttgcgagc accctttag aaatgcaca tttcccact 
tacgcagta aaactgagg acgagttaa atcgtctcc 
tgcgtatgg ttgtggaca ttgacaata tgggtacgg 
caatgagtc gtgaagaaa acattgaaa caaaaaaag 
tttacacgt acttattcc acttgttgc gtaaatgca 
tcctaactc ttgacactc ccgtcgttg agagttcgc 
tcggacagc ccccatttg aaaaaccgt gcattcaca 
aagcgatca caaatttgc tagtttaca aattctcac 
atttggccc aacaagagt ggaagacga tgtatgaag 
tggctagga ttctcctcc actgtttga acacgtggg 
agtttccga gtccgagca gccagaagc atattgtaa 
gtccggagg aagccacag tttataaaa tggaacata 
tgcgttggt tggtgtttt gcaacgttc caacatttt 
gcgactcgt tgaaacatt acgatacga caatggtgt 
ttgggagga cgcttctaa gggctgttt tcaaataca 
tgaggaagc aaatcatgg atcagttta atcgagagc 
gggacaaaa tgccccaag aacgtcgcg ttatgctcc 
tcacacatt ttcccccgt ttctgaaca cgttggatg 
aagagattt acgcgcagg attccccga tttgggatc 
ctgatttta aacaaggac cgccgcaac atttccaga 
atttattcg aagcgggga aaaacaaaa cgaatgcca 
agcgaggag cgagcgatt tggacgcga aagcgggca 
aaactgggg atgtaccga gtcgagaga cgaaacgta 
ggagggcac aggatgaac agtttgagg atattccga 
tggagacgg ttgcacaaa ttattgcaa caattgatg 
atccgaatt acatgaccg gacctgatc tgattttct 
ccgagaatg cgcccgcca cagacgaag cgcccaaac 
ttttagaaa aacgcaaag tgattgaaa tgcacgtca 
ttggtaaca gtttgaccg aaacggtct cgaaacaga 
ccatgttgt ggttcattg gtctacaaa acgtctttt 
cgcttcaat tgccccgaa tgccgatcg aatacatca 
cccaattgt tattcgatc caaaacacc gcaccattg 
ccctaatta aagaaaacc gtccaatcg gcggtattt 
gtttccgcc cgtttgaaa ctcaaggtt tcacagaga 
ttgtttttt gcgcggtca ctttcagcg aacagatca 
aaggttacg gaacgcatt taatgaacc atttgaaag 
actaccaca ttcgtaaag gcattctcc actttctga 
ctttcgcag acgagaacg cgattggtt atttcgaac 
gtcagggat tttcgaaag atattaaat taaaccggt 
cgatttatc aaggctata ttaaggact atgaaggcg 
ccactctca tgcattgcg aactgaacc ccaaaataa 
tctacaccg agttcactt cagtcgttc ggaggcaaa 
aaaagttcg tcaaacagt tgatttatc agcagcaaa 
ggaacgtat caaaggttc accccggtc tgactttct 
tcaaagagg gggtgaaga gcatcccca ttgagtcca 
ttccggggc tgcaggaga caggctgga aaccggcag 
cgagggcca ccagagggg cgcaacaac tggaggagt 
ctcaagata ttgacgtgt tggctgata tgctcaaaa 
gtgtgcttc aagcggtga aaagtcacg aagattctt 
ggccgtttc gactgccag ttgataaaa acgacgttc 
cggattatt acgaccaca tcaagtatc ccatggatt 
tgaaaacaa tggccgatc gcttgaagt cacggtatt 
acgtttcaa ggcgtttgt ttatcgctg atatgatgc 
ggattttcc gcaattgta aaatttata attcgcctg 
agacagagt attaccagt gtgctgtga acttgcagc 
agtatttcc agaccaaga tgaaggaag tcggtttgt 
gggacaaat aatttaaga gaaaaccaa tatatttag 
tgttttata cattacata ataaaacga gtatgaatt 
ataataata ataataaaa atgactagt aaac

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY