Status
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Gene information
General information
TC013609 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3159023..3164066
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013609
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10132

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head appendages swollen - Penetrance (percentage of animals/eggs on slide): more than 80%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    legs swollen - Penetrance (percentage of animals/eggs on slide): more than 80%

    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen cuticle dorsal centralstructure not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    posterior terminus partially not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013609 RA
      MLTSSSHVS NNGSYSNDR GAGLEAEYV VDPASGGFF 
      HTDFKKHDD LKEMLDSNK DSLKLEAMK RIIGMVAKG 
      RDASMLFPA VVKNVVSKN IEVKKLVYV YLERYAEEQ 
      QDLALLSIS TFQRALKEP NQLIRAGAL RVLSSIRVK 
      MISPIVMLA IRDASSDMS PYVRKTAAH AIPKLYSLE 
      NELKSELVT IIQKLLADK TVLVIGSAV MAFTEVCPE 
      RVDLIHQVY RKLCALLVD VDEWGQVTI VNMLTRYAR 
      MQFADPNLH SRGDDSDRP FYDSGSDSS EKPAPTLDP 
      DHRLLLRST RPLLQSRNA AVVMAVAQL YHHAAPKSE 
      SPLAAKAMV RLLRSHVEV QSVVLNAIA SISAQNKGM 
      FEAYLKSFF VRTSDPTNI KLLKLEILT NLTTDSNVS 
      IILRELQTY ISNSDKKFV AATIQAIGR CACSINEVT 
      DSCLSGLVS LLSNRDEAV VAESVVVIK KLLQTQAAD 
      PKEIITHMA RLLDSITVA QARAAILWL LGEHSQKVP 
      NIAPDILRK LAKTFSDEH DIVKLQVMN LAVKLYITN 
      SEQTALLCQ YIFNLARYD QNYDIRDKA RLFKQFIPQ 
      QNGRIASQS ARIFLASKP APLLQSHFK DHEDLQLGS 
      LSHYIKQRA TGYEPLPPF PENPPPGDV RNVEPIVIE 
      DSKSYKKSD KKGKDMFLW ESGSSRGAS SNNSSDSSD 
      SSSSSSDSS SEDSESQYE EESSSDEKS ASCTSSEES 
      SSEESEEEA KKPEAPKPK SNLDLLLDL GDVDSTAPV 
      MTPSLGGFL TPSVVSTTP SQVEMTPPV FTNTKTIEL 
      INKINGRGL SATYRFTRT PHILSPSMA NINIMFTNN 
      TTEDISDIR LGKKNLGLD MSMYEFANI ARLPPNGCL 
      PASLGIDFN DTTQPANFE IVCSLGNFS VCIKPTIGE 
      LVRPVRMDT PTFQEKQSK LGGMNEHIG KVKCVSNIV 
      ERVTECSNL GVCDPASDS LRFAGQTLK SSNFVLVTI 
      TKQEVVTVN CENMVFGSV MLNELLHNL KS
    CDS sequence
    • >TC013609 RA
      atgttgacg tcgtcctcg catgtgtcc aacaatggc 
      tcgtactcg aatgacagg ggggccggt ctggaggct 
      gaatatgtg gttgatccg gcctccgga ggcttcttc 
      cacaccgat ttcaagaag cacgacgac ctcaaggaa 
      atgctggat tctaataaa gacagtctt aagttggag 
      gccatgaag cgcatcatt gggatggtg gcgaagggg 
      agggacgcg tctatgttg ttcccggct gttgtcaaa 
      aacgttgtt tctaagaat attgaagtt aagaaattg 
      gtttatgtt tatttggag agatacgcg gaagagcag 
      caggatttg gcgctactg tcgatttcc acgtttcaa 
      agagcttta aaagaaccg aatcagttg atacgagcg 
      ggcgctttg agggtgtta tcaagtatt agggttaag 
      atgatttcg ccgattgtt atgctggcg ataagggac 
      gcctcgtcc gatatgtcc ccttacgtg cggaaaaca 
      gcggcccat gctatcccc aaactttat agcttagaa 
      aacgagctg aaatcggaa ctagtaaca ataatccag 
      aagctccta gctgacaaa accgttcta gtgataggt 
      tccgccgtg atggccttc acagaagtg tgccccgaa 
      cgcgtggac ttaatccac caagtctac cggaaactt 
      tgtgccttg ttagtagac gtggacgaa tggggccaa 
      gtcacaatc gtgaacatg ctgacgcgc tacgcgcgc 
      atgcagttc gccgacccc aacctccac agccgcggc 
      gacgactcc gaccgtcca ttttacgat tcaggttcc 
      gactcctcg gagaagccg gcccccacg ctggacccc 
      gaccaccgg ctgctgctg cgctcgacg cggcccctc 
      ctgcagtcg cgcaacgcc gccgtggtc atggccgtg 
      gcgcagctc taccaccac gccgccccc aagagcgag 
      agccccctg gcggccaag gcgatggtg cggctgttg 
      cgctcgcac gtcgaggtg cagagcgtc gttctgaac 
      gccatcgcc tccatcagt gcccagaac aagggcatg 
      tttgaggcg tacttgaag agcttcttc gtccggacg 
      tcagatccg accaatatt aagctactc aaacttgag 
      attctgacg aatctcacc accgattcc aacgtctcg 
      attatcttg agggagctg caaacttac atttccaac 
      agcgataaa aagtttgta gcggccacg attcaggcg 
      atagggcgg tgtgcatgc tctattaat gaagtcacg 
      gactcttgt cttagcggg cttgtctcc ttgctttcg 
      aacagagac gaagccgtt gttgccgaa agtgtggtc 
      gttatcaaa aaactgctt caaacgcag gctgccgac 
      cctaaagag ataatcacc cacatggcg cgactcctt 
      gacagcata acagtggct caggcccgc gccgcaatt 
      ctgtggcta cttggcgaa cattcgcaa aaagtccca 
      aacatagcg cctgacatt ctccggaaa ttagccaaa 
      accttctct gacgaacac gacattgtc aaattacaa 
      gtaatgaac ttggctgta aaactatac ataacaaac 
      tccgaacaa acggcatta ttgtgccaa tatatcttt 
      aatttggcc cggtatgat caaaactac gacattagg 
      gacaaagca aggctgttt aagcagttc ataccgcaa 
      caaaacggc cgaattgcc tcccaatcg gctaggata 
      ttcctggca agcaagccc gcaccccta cttcagtcg 
      catttcaaa gaccacgaa gacttgcaa ttgggctcc 
      ctgtcgcac tatatcaag caacgagcg accggctat 
      gagcccctg cccccgttc ccggagaat ccgcccccg 
      ggtgacgtc cgaaacgtc gaaccaatc gtaattgaa 
      gactccaaa tcgtacaaa aaatcggac aaaaagggt 
      aaagacatg ttcttgtgg gagtcgggg agttcccgg 
      ggggctagt agcaacaac agctcggac agctccgac 
      tcgtctagc tctagtagc gacagttcg agtgaagac 
      agcgaaagt caatatgag gaagaatcg tcaagtgat 
      gagaagtcg gcgagttgc acttcgagc gaagagtcc 
      agcagtgag gagagtgaa gaagaagcg aagaaaccc 
      gaagcacca aaaccaaaa tcaaactta gacttattg 
      ctagattta ggcgacgtt gatagcact gcacccgtg 
      atgacccca tcactgggc gggttccta acaccgagt 
      gtcgtaagc acgacacct tcgcaggtc gaaatgacg 
      ccccccgtt ttcaccaac actaaaacc atcgaactt 
      attaacaaa atcaacggg cgggggctg tcggccact 
      tacaggttc acccggacg cctcatatt ttatcgcca 
      agtatggca aacatcaat ataatgttc accaacaac 
      accaccgaa gacatcagt gatattagg ctgggaaaa 
      aagaattta ggactggac atgtcgatg tatgagttc 
      gcaaacatc gccagattg ccgccaaac ggctgcctg 
      cccgcgtcg ctcgggatc gacttcaac gacacgacc 
      cagccggcg aacttcgag atcgtgtgc tcgttgggc 
      aacttcagc gtctgcata aagccgacc ataggcgag 
      ctggtgcgc cccgtccgg atggacacc cccacattc 
      caggaaaag cagtcgaag ttgggcggc atgaacgaa 
      catatcggc aaagtgaag tgcgtctct aacatcgtc 
      gaaagagtc accgaatgc agcaacttg ggcgtgtgc 
      gacccagcg agcgacagc ctgaggttc gcgggccaa 
      acgctgaaa tcgtcgaac ttcgttctg gtcacaata 
      accaagcag gaggtggtc actgtcaat tgcgagaac 
      atggtcttt gggtccgtt atgctcaac gaactgcta 
      cataatctc aagtcataa
    mRNA sequence
    • >TC013609 RA
      aaactcacc taaacgcac acgttggat gctggaagc 
      gtctcaaaa tcagggaag ccccaaatt gccaacttc 
      tgcactttc aaaattcac cgacagtac cgccaactt 
      gaaatttgt gttctaatt tacaaaatt attttttac 
      gatttaact gggcgcgtt gactttgca atatttaac 
      aagtaacaa gcggatggg gcaaattgt gcccctttt 
      ctcggattt ttacgtgcg gtcgcaacg caattcgcg 
      gttggcgcc actgtgtaa cctaaaaat cgtacgttt 
      cgtgtaatg gacgtgtca ttttcggtc gtgttcatg 
      cttaattcg cattaatgg tattgttaa ggccgtttg 
      gtgagacag agtttggat ttttattat gttgacgtc 
      gtcctcgca tgtgtccaa caatggctc gtactcgaa 
      tgacagggg ggccggtct ggaggctga atatgtggt 
      tgatccggc ctccggagg cttcttcca caccgattt 
      caagaagca cgacgacct caaggaaat gctggattc 
      taataaaga cagtcttaa gttggaggc catgaagcg 
      catcattgg gatggtggc gaaggggag ggacgcgtc 
      tatgttgtt cccggctgt tgtcaaaaa cgttgtttc 
      taagaatat tgaagttaa gaaattggt ttatgttta 
      tttggagag atacgcgga agagcagca ggatttggc 
      gctactgtc gatttccac gtttcaaag agctttaaa 
      agaaccgaa tcagttgat acgagcggg cgctttgag 
      ggtgttatc aagtattag ggttaagat gatttcgcc 
      gattgttat gctggcgat aagggacgc ctcgtccga 
      tatgtcccc ttacgtgcg gaaaacagc ggcccatgc 
      tatccccaa actttatag cttagaaaa cgagctgaa 
      atcggaact agtaacaat aatccagaa gctcctagc 
      tgacaaaac cgttctagt gataggttc cgccgtgat 
      ggccttcac agaagtgtg ccccgaacg cgtggactt 
      aatccacca agtctaccg gaaactttg tgccttgtt 
      agtagacgt ggacgaatg gggccaagt cacaatcgt 
      gaacatgct gacgcgcta cgcgcgcat gcagttcgc 
      cgaccccaa cctccacag ccgcggcga cgactccga 
      ccgtccatt ttacgattc aggttccga ctcctcgga 
      gaagccggc ccccacgct ggaccccga ccaccggct 
      gctgctgcg ctcgacgcg gcccctcct gcagtcgcg 
      caacgccgc cgtggtcat ggccgtggc gcagctcta 
      ccaccacgc cgcccccaa gagcgagag ccccctggc 
      ggccaaggc gatggtgcg gctgttgcg ctcgcacgt 
      cgaggtgca gagcgtcgt tctgaacgc catcgcctc 
      catcagtgc ccagaacaa gggcatgtt tgaggcgta 
      cttgaagag cttcttcgt ccggacgtc agatccgac 
      caatattaa gctactcaa acttgagat tctgacgaa 
      tctcaccac cgattccaa cgtctcgat tatcttgag 
      ggagctgca aacttacat ttccaacag cgataaaaa 
      gtttgtagc ggccacgat tcaggcgat agggcggtg 
      tgcatgctc tattaatga agtcacgga ctcttgtct 
      tagcgggct tgtctcctt gctttcgaa cagagacga 
      agccgttgt tgccgaaag tgtggtcgt tatcaaaaa 
      actgcttca aacgcaggc tgccgaccc taaagagat 
      aatcaccca catggcgcg actccttga cagcataac 
      agtggctca ggcccgcgc cgcaattct gtggctact 
      tggcgaaca ttcgcaaaa agtcccaaa catagcgcc 
      tgacattct ccggaaatt agccaaaac cttctctga 
      cgaacacga cattgtcaa attacaagt aatgaactt 
      ggctgtaaa actatacat aacaaactc cgaacaaac 
      ggcattatt gtgccaata tatctttaa tttggcccg 
      gtatgatca aaactacga cattaggga caaagcaag 
      gctgtttaa gcagttcat accgcaaca aaacggccg 
      aattgcctc ccaatcggc taggatatt cctggcaag 
      caagcccgc acccctact tcagtcgca tttcaaaga 
      ccacgaaga cttgcaatt gggctccct gtcgcacta 
      tatcaagca acgagcgac cggctatga gcccctgcc 
      cccgttccc ggagaatcc gcccccggg tgacgtccg 
      aaacgtcga accaatcgt aattgaaga ctccaaatc 
      gtacaaaaa atcggacaa aaagggtaa agacatgtt 
      cttgtggga gtcggggag ttcccgggg ggctagtag 
      caacaacag ctcggacag ctccgactc gtctagctc 
      tagtagcga cagttcgag tgaagacag cgaaagtca 
      atatgagga agaatcgtc aagtgatga gaagtcggc 
      gagttgcac ttcgagcga agagtccag cagtgagga 
      gagtgaaga agaagcgaa gaaacccga agcaccaaa 
      accaaaatc aaacttaga cttattgct agatttagg 
      cgacgttga tagcactgc acccgtgat gaccccatc 
      actgggcgg gttcctaac accgagtgt cgtaagcac 
      gacaccttc gcaggtcga aatgacgcc ccccgtttt 
      caccaacac taaaaccat cgaacttat taacaaaat 
      caacgggcg ggggctgtc ggccactta caggttcac 
      ccggacgcc tcatatttt atcgccaag tatggcaaa 
      catcaatat aatgttcac caacaacac caccgaaga 
      catcagtga tattaggct gggaaaaaa gaatttagg 
      actggacat gtcgatgta tgagttcgc aaacatcgc 
      cagattgcc gccaaacgg ctgcctgcc cgcgtcgct 
      cgggatcga cttcaacga cacgaccca gccggcgaa 
      cttcgagat cgtgtgctc gttgggcaa cttcagcgt 
      ctgcataaa gccgaccat aggcgagct ggtgcgccc 
      cgtccggat ggacacccc cacattcca ggaaaagca 
      gtcgaagtt gggcggcat gaacgaaca tatcggcaa 
      agtgaagtg cgtctctaa catcgtcga aagagtcac 
      cgaatgcag caacttggg cgtgtgcga cccagcgag 
      cgacagcct gaggttcgc gggccaaac gctgaaatc 
      gtcgaactt cgttctggt cacaataac caagcagga 
      ggtggtcac tgtcaattg cgagaacat ggtctttgg 
      gtccgttat gctcaacga actgctaca taatctcaa 
      gtcataagt tgcttcagc gtatttgat gtattgtac 
      ttgtttgta acgggaaat acttgaaca taatgtgca 
      cttgctaaa cgccgagct tccttttgc gtgaatatt 
      atgttggat gtatttgcc acctagtta tcacttttc 
      ttttttatc ctttcaaat aaatattta tgagaagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY