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Gene information
General information
TC033256
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013038
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14797654..14801345
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033256
    iBeetle screen iB_10131

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033256 RA
      MREHQTLQK QVKKCGHIN QIASSSRSS KSAYSTAQN 
      AIINAFQKT VSNIQRKCP HVANKNRQN VQTSKNQTG 
      NMIKRPCGH VVNNNTNKQ VLRINCPHK NTAQNSNNS 
      EGQKMINVP QPAKLSVQF TNQATAETS PPQTQKNKV 
      AEPSTKNKT TGVPPKKPT PTPQKTPPN DPKPCDKIT 
      EIFKRPENT MEETEASNN SSFQKVTQE LANIIKNFQ 
      KQIHEVPIT KAKLEEIPS AMIQPKVKE FFKKTEPPV 
      PKNQPTNKT PQKSVSNNA KTNQNGVKK QSSLRNFTP 
      SNNTLAEAS SVRRFSSST NHALSLKSS STSTVSKRL 
      CKRSSFLNV DPLKQLKDE VHKAIRDRK TFTVRGHFG 
      PVRKALLER GWVEKFHVS YKDQLSEDL KRYQNYSIN 
      ELLPLMRRE DLTSLCKSL IKSKLLGGY QVDFYWGTN 
      DDAFKENPD KIKYTMINK LKREIFSYT NKQGLCDAS 
      KSCYWYQKN NVSKLNHPR SYSLGKNGD LKGFVDDFN 
      VTAAMSLLK WVVKVNVTK ESKLLSPSG KIPLETFDF 
      ALNECCKFI KKGRHEDID VQIPEATET EWNQFLEYF 
      YKIVHYSNH FKSEGTETE DSLVRKSNY ILNSLRSFY 
      PFLDMDGMM NIWILKPTA SSRGRGIHM CRTLQYVLK 
      VVKQNSNIR YIIQKYIER PLLIYNTKF DIRQWFLIS 
      NCSPLTIWM YKDCYLRFS SQTYNLRKL HESIHLTNN 
      SVQCKYKNA SLDTALPSY NMWDSNDFQ RYLANIGYP 
      NAFKEIVYP GMKQSITAA VMMHQDKMV VRRNSFELY 
      GADFILTED FQPWLLEIN SNPALFAST PVTARMCPK 
      VLEDVVKVV IDYGHNNKA STGLFELIY KEPLTKLPP 
      SGTLELKGL PLQSNYFFN APDQESSDV FSAFKFSNS 
      NNSALVVDE AQDCLKTFG EEIKKTLKN LLQIIHNER 
      EKREAKLNQ NLSVKV
    CDS sequence
    • >TC033256 RA
      atgcgtgaa caccaaaca ttacaaaaa caagtaaaa 
      aaatgcgga cacatcaat caaattgca tcttcaagc 
      agaagcagc aaatcagca tattccaca gcacaaaat 
      gccatcatt aacgccttc cagaagact gtctcaaac 
      attcaaaga aaatgtccc catgttgcg aacaaaaac 
      cgacaaaat gtccaaact agcaaaaat caaaccgga 
      aacatgatt aagagacct tgcggtcac gttgttaat 
      aataatact aataagcaa gtgttaaga ataaattgc 
      cctcacaaa aacacagca caaaacagt aacaactcc 
      gagggccaa aaaatgata aatgttcct caacctgca 
      aaactttca gtccagttt actaaccaa gctacagct 
      gaaacctcg ccacctcaa acacaaaag aacaaagtt 
      gcagaacca agcaccaaa aacaaaacc acaggtgtg 
      cccccaaaa aagcccacc ccaaccccc caaaaaacg 
      cccccaaat gacccaaaa ccgtgcgat aagatcacc 
      gaaattttc aaacgaccg gaaaataca atggaagaa 
      acagaagct tcaaataat tcgtcattt cagaaggta 
      acacaagag cttgcaaat ataataaaa aatttccaa 
      aaacaaatc catgaagtt ccgattacc aaagccaaa 
      ctggaggag attccttct gcgatgata cagccgaaa 
      gttaaggag tttttcaaa aaaactgaa cctccagtt 
      ccgaaaaat caaccaacg aataaaacg ccacaaaaa 
      agtgtttcc aataacgcg aaaactaat caaaatggt 
      gtgaaaaag cagtcgagt ttgcggaat tttaccccg 
      agtaacaat actttggcg gaggccagc agtgtgagg 
      cgattttcc tcgtccaca aaccacgct ctaagcctg 
      aaatcgagc tcgacaagc actgtgagc aaaagactt 
      tgcaaacgc tcgagtttc cttaacgtc gatccgtta 
      aaacagctc aaagacgaa gtgcataaa gcgatccgt 
      gaccgaaaa accttcaca gtccgtggt catttcggg 
      ccggtccgg aaggccctt cttgagcga ggctgggtc 
      gagaaattt cacgtctca tacaaggac caactaagc 
      gaggatttg aaacgttac cagaactat tcaatcaac 
      gaactgttg ccgctgatg agacgtgaa gacttgaca 
      agcctttgc aaaagtcta atcaaatcg aagttatta 
      ggcggctac caggtcgac ttttactgg ggcaccaac 
      gatgacgcc ttcaaggaa aaccccgat aaaattaaa 
      tacaccatg atcaataaa ctgaagcgg gagattttc 
      agttacacg aataagcaa ggcttgtgc gatgccagt 
      aaaagttgt tactggtac cagaaaaac aacgtttcg 
      aaactgaac catcccaga tcgtacagt ttgggtaaa 
      aacggtgat ttgaaaggg tttgttgat gattttaac 
      gtaaccgcc gccatgtct ctactcaaa tgggtggtg 
      aaagtgaat gtgactaaa gagtcgaag ctgttgagc 
      cccagtggg aaaataccg cttgaaacg ttcgatttt 
      gccctaaat gagtgttgc aaatttatc aaaaagggg 
      cgacatgag gacattgat gtgcaaata ccggaagca 
      acagagact gaatggaac cagtttttg gaatatttt 
      tacaaaatc gtgcactat agtaatcat tttaagagc 
      gagggcacg gagactgag gattcgttg gtgcgaaaa 
      tcaaattat attttgaac agtttgagg agtttttac 
      ccgtttctt gacatggac gggatgatg aatatttgg 
      attttgaaa ccgactgct agcagcaga ggccggggg 
      attcacatg tgtaggacg ttgcagtac gttttgaaa 
      gttgtcaag caaaactca aatattaga tacatcatc 
      cagaaatat atcgagcgg cctctcctg atttacaac 
      acgaaattc gacataaga cagtggttt ttgatcagt 
      aattgcagt ccactgacg atatggatg tacaaggac 
      tgttattta cgcttcagc tcccagact tacaacctt 
      cgcaaattg cacgaatcc atccacttg acaaataat 
      tcggttcag tgcaaatac aagaacgcc tcgttggac 
      acagcactg ccgagttac aacatgtgg gactcaaac 
      gatttccag agatatttg gcaaatatc ggttatcca 
      aacgccttc aaagagatc gtttatccg ggaatgaaa 
      cagagcata accgctgcg gtaatgatg catcaggat 
      aaaatggtc gttcggcga aattctttc gagttgtac 
      ggcgccgat tttattcta actgaagac tttcaaccg 
      tggttgttg gagattaat tccaatccg gccctgttt 
      gcctcaacg ccagtgacg gcaaggatg tgtccgaag 
      gtgctcgag gatgttgtc aaagttgtt atcgattac 
      gggcataat aataaggcc agtaccgga ctgtttgaa 
      ctgatctac aaggagcct ttgacaaaa ctgcctcct 
      tctggtact ttagaactt aagggtttg ccgctgcaa 
      agcaactac tttttcaat gctcctgat caagaatcg 
      agtgatgtt ttcagtgct tttaagttt agtaacagt 
      aataatagt gctttggtg gtggacgag gcccaagac 
      tgtttgaag acgtttggg gaagagatc aagaaaacg 
      ttaaagaat ctgttgcag attatacac aatgagagg 
      gaaaaacgc gaggcgaag ctgaaccag aatcttagt 
      gttaaagtt tga
    mRNA sequence
    • >TC033256 RA
      ttaagcggc ttattatgt caaaattgt aaggtgttg 
      atcatccat tatcaaaat tacccgttt cccattaaa 
      ttgttcagt atgcgtgaa caccaaaca ttacaaaaa 
      caagtaaaa aaatgcgga cacatcaat caaattgca 
      tcttcaagc agaagcagc aaatcagca tattccaca 
      gcacaaaat gccatcatt aacgccttc cagaagact 
      gtctcaaac attcaaaga aaatgtccc catgttgcg 
      aacaaaaac cgacaaaat gtccaaact agcaaaaat 
      caaaccgga aacatgatt aagagacct tgcggtcac 
      gttgttaat aataatact aataagcaa gtgttaaga 
      ataaattgc cctcacaaa aacacagca caaaacagt 
      aacaactcc gagggccaa aaaatgata aatgttcct 
      caacctgca aaactttca gtccagttt actaaccaa 
      gctacagct gaaacctcg ccacctcaa acacaaaag 
      aacaaagtt gcagaacca agcaccaaa aacaaaacc 
      acaggtgtg cccccaaaa aagcccacc ccaaccccc 
      caaaaaacg cccccaaat gacccaaaa ccgtgcgat 
      aagatcacc gaaattttc aaacgaccg gaaaataca 
      atggaagaa acagaagct tcaaataat tcgtcattt 
      cagaaggta acacaagag cttgcaaat ataataaaa 
      aatttccaa aaacaaatc catgaagtt ccgattacc 
      aaagccaaa ctggaggag attccttct gcgatgata 
      cagccgaaa gttaaggag tttttcaaa aaaactgaa 
      cctccagtt ccgaaaaat caaccaacg aataaaacg 
      ccacaaaaa agtgtttcc aataacgcg aaaactaat 
      caaaatggt gtgaaaaag cagtcgagt ttgcggaat 
      tttaccccg agtaacaat actttggcg gaggccagc 
      agtgtgagg cgattttcc tcgtccaca aaccacgct 
      ctaagcctg aaatcgagc tcgacaagc actgtgagc 
      aaaagactt tgcaaacgc tcgagtttc cttaacgtc 
      gatccgtta aaacagctc aaagacgaa gtgcataaa 
      gcgatccgt gaccgaaaa accttcaca gtccgtggt 
      catttcggg ccggtccgg aaggccctt cttgagcga 
      ggctgggtc gagaaattt cacgtctca tacaaggac 
      caactaagc gaggatttg aaacgttac cagaactat 
      tcaatcaac gaactgttg ccgctgatg agacgtgaa 
      gacttgaca agcctttgc aaaagtcta atcaaatcg 
      aagttatta ggcggctac caggtcgac ttttactgg 
      ggcaccaac gatgacgcc ttcaaggaa aaccccgat 
      aaaattaaa tacaccatg atcaataaa ctgaagcgg 
      gagattttc agttacacg aataagcaa ggcttgtgc 
      gatgccagt aaaagttgt tactggtac cagaaaaac 
      aacgtttcg aaactgaac catcccaga tcgtacagt 
      ttgggtaaa aacggtgat ttgaaaggg tttgttgat 
      gattttaac gtaaccgcc gccatgtct ctactcaaa 
      tgggtggtg aaagtgaat gtgactaaa gagtcgaag 
      ctgttgagc cccagtggg aaaataccg cttgaaacg 
      ttcgatttt gccctaaat gagtgttgc aaatttatc 
      aaaaagggg cgacatgag gacattgat gtgcaaata 
      ccggaagca acagagact gaatggaac cagtttttg 
      gaatatttt tacaaaatc gtgcactat agtaatcat 
      tttaagagc gagggcacg gagactgag gattcgttg 
      gtgcgaaaa tcaaattat attttgaac agtttgagg 
      agtttttac ccgtttctt gacatggac gggatgatg 
      aatatttgg attttgaaa ccgactgct agcagcaga 
      ggccggggg attcacatg tgtaggacg ttgcagtac 
      gttttgaaa gttgtcaag caaaactca aatattaga 
      tacatcatc cagaaatat atcgagcgg cctctcctg 
      atttacaac acgaaattc gacataaga cagtggttt 
      ttgatcagt aattgcagt ccactgacg atatggatg 
      tacaaggac tgttattta cgcttcagc tcccagact 
      tacaacctt cgcaaattg cacgaatcc atccacttg 
      acaaataat tcggttcag tgcaaatac aagaacgcc 
      tcgttggac acagcactg ccgagttac aacatgtgg 
      gactcaaac gatttccag agatatttg gcaaatatc 
      ggttatcca aacgccttc aaagagatc gtttatccg 
      ggaatgaaa cagagcata accgctgcg gtaatgatg 
      catcaggat aaaatggtc gttcggcga aattctttc 
      gagttgtac ggcgccgat tttattcta actgaagac 
      tttcaaccg tggttgttg gagattaat tccaatccg 
      gccctgttt gcctcaacg ccagtgacg gcaaggatg 
      tgtccgaag gtgctcgag gatgttgtc aaagttgtt 
      atcgattac gggcataat aataaggcc agtaccgga 
      ctgtttgaa ctgatctac aaggagcct ttgacaaaa 
      ctgcctcct tctggtact ttagaactt aagggtttg 
      ccgctgcaa agcaactac tttttcaat gctcctgat 
      caagaatcg agtgatgtt ttcagtgct tttaagttt 
      agtaacagt aataatagt gctttggtg gtggacgag 
      gcccaagac tgtttgaag acgtttggg gaagagatc 
      aagaaaacg ttaaagaat ctgttgcag attatacac 
      aatgagagg gaaaaacgc gaggcgaag ctgaaccag 
      aatcttagt gttaaagtt tgaataaaa atgtttcaa 
      tttgttggg ggggtttga aagaaaatt gagtcctga 
      atcagattt attaataga aaaaataaa ataaataca 
      atgtgtttt agaac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY