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Gene information
General information
TC013060 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14660863..14663265
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013060
    iBeetle screen iB_10127

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013060 RA
      MYKVLRNVT RFKLTVSTK IPTLRTLSV IPTPEYIFK 
      RHTSSYKSD NVYLVDEDD SSGNETGNN INKYTQRTH 
      TCGELRIDN VGQRVVLCG WLEFQRMNK FAVLRDSYG 
      ATQLLVPEE DTKTQKLLQ NLPFESIIQ VKGTVLSRP 
      TDMKNKKQP TGDIEIFIE HFTVLNKAK DSLPFHIRE 
      HHKAKEALR MQYRYLDLR YPLMQRNLR VRSEMLMKM 
      REFLVNHSG FVDVETPTL FKATPGGAQ EFIVPTRFP 
      GQFYSLVQS PQQFKQMLM AGAVDRYFQ IARCYRDEG 
      ARPDRQPEF TQLDIEMSF TDLDNILQL VEELLGHAW 
      PPFLGPLPK KFQRMGFGE AMELYGSDQ PDTRFGFKL 
      ENCTELLKL NEKFINSDD FGAYCIVFP KEYANLNKG 
      VKENLEKIS KQFPLVKFV QTKVATPGE LAPKLGKLL 
      GDNVAAKLA NHVSDDCVL FLGFGNKKQ TQSLMGKVR 
      LEYANHLEQ LGVSVRAKG MHLLWVVDM PLFEPGESP 
      GSLQSAHHP FTAPNPDDL LLLDTAPLQ VRSLSFDLV 
      LNGNEIGGG SVRIHDPDL QEKIFRMLN IDKNSMQHI 
      IDMLSSGCP PHGGIALGL DRLMSKILN TASIRDVIA 
      FPKTFEGRD PLSGAPSAV SDADLKLYH VKSVVE
    CDS sequence
    • >TC013060 RA
      atgtacaaa gttttaagg aatgtcacg agatttaaa 
      ctaactgtc tcaacgaaa atcccaacc ctacgaaca 
      ctgtcagtc atacccacc cccgagtat attttcaag 
      cgacacact tctagttac aaatcagat aacgtttat 
      ttagtggac gaagatgat agttcgggt aacgaaaca 
      ggcaataat atcaataag tacacacaa aggacgcac 
      acttgtggc gaattaaga attgacaac gtgggtcag 
      agagtggtt ctatgtggc tggttggag tttcaaaga 
      atgaataaa tttgccgtc cttagagac agttacggc 
      gccacccaa ctcctagtc cccgaagaa gacaccaag 
      acgcaaaaa ttgttacaa aatctcccc tttgaatcg 
      ataatccaa gtcaaaggc accgtcttg tcgcgtcca 
      actgacatg aaaaacaag aaacaacca actggcgat 
      attgaaatt ttcatcgaa cacttcact gttctaaat 
      aaggccaaa gacagcctc cccttccac atccgagaa 
      caccacaag gctaaagaa gccctccgt atgcaatac 
      cgctacctg gacttacgt tacccccta atgcagcgc 
      aatttacgc gtccgttcc gaaatgctc atgaaaatg 
      cgcgaattt ctcgtaaac cacagcggt tttgtcgat 
      gtcgagacg ccaactctg ttcaaggcc acgcctggg 
      ggggcgcaa gaattcatc gtgccaacg cgattccca 
      ggccagttc tattcccta gtccagagc ccccaacaa 
      ttcaaacaa atgctaatg gcgggggct gttgacagg 
      tactttcaa atcgcacgg tgttaccga gacgagggg 
      gcgcggcct gaccgccag cccgagttc acgcaactc 
      gatatcgaa atgtctttc actgacttg gacaacatc 
      ttgcaacta gttgaggag ttgttgggg cacgcgtgg 
      cccccattt ttgggacct ttgcccaaa aaattccaa 
      aggatgggg ttcggggag gctatggaa ttgtacggg 
      tcggatcag cccgatacc aggttcggg tttaagttg 
      gaaaattgt accgagctt ttgaaactg aacgaaaaa 
      ttcattaat tcggacgat tttggggct tattgcatt 
      gtcttccca aaagaatac gccaatttg aataaggga 
      gtgaaggaa aatttggaa aaaattagc aaacagttt 
      ccccttgtt aagttcgtt caaactaaa gtagcgaca 
      cctggtgaa ttggcgccc aaacttggg aaattgttg 
      ggcgataac gtggcggca aaattagcc aatcacgtg 
      agcgacgat tgtgttttg tttttaggt ttcgggaat 
      aaaaaacaa actcaaagc ttgatggga aaagttagg 
      cttgaatat gccaatcat ttggaacaa ctaggtgtg 
      agtgtgcgt gcaaaaggc atgcacttg ttgtgggtg 
      gtggacatg ccgttattc gaaccgggg gaaagcccg 
      ggaagtctt caatcggca catcaccca ttcacagcg 
      ccgaatcct gatgattta ctcctattg gatacggcg 
      cctttgcag gtgcggtct ttgtcattc gatttggtt 
      ttgaacggg aatgagata gggggaggt tcagtacgt 
      attcatgac cccgatctt caagaaaaa attttccgg 
      atgttaaac attgataaa aattcgatg caacatatt 
      atcgatatg ttatcatca gggtgtccg ccccatggg 
      ggcattgcg ctgggactc gatcgtctc atgtcgaaa 
      attttaaat acggcgagt attcgtgat gtgatcgcg 
      tttcccaaa acattcgaa ggtcgggat ccgttgagt 
      ggggcgccg tcagctgtt tccgacgcc gacttgaag 
      ctctaccat gtcaagagt gttgttgag tga
    mRNA sequence
    • >TC013060 RA
      caatcagcg ctgtcaaat agttgtcaa agagaattt 
      tgaaaaaat atgagccgg tttagaata acaattagg 
      tgaaaatgt acaaagttt taaggaatg tcacgagat 
      ttaaactaa ctgtctcaa cgaaaatcc caaccctac 
      gaacactgt cagtcatac ccacccccg agtatattt 
      tcaagcgac acacttcta gttacaaat cagataacg 
      tttatttag tggacgaag atgatagtt cgggtaacg 
      aaacaggca ataatatca ataagtaca cacaaagga 
      cgcacactt gtggcgaat taagaattg acaacgtgg 
      gtcagagag tggttctat gtggctggt tggagtttc 
      aaagaatga ataaatttg ccgtcctta gagacagtt 
      acggcgcca cccaactcc tagtccccg aagaagaca 
      ccaagacgc aaaaattgt tacaaaatc tcccctttg 
      aatcgataa tccaagtca aaggcaccg tcttgtcgc 
      gtccaactg acatgaaaa acaagaaac aaccaactg 
      gcgatattg aaattttca tcgaacact tcactgttc 
      taaataagg ccaaagaca gcctcccct tccacatcc 
      gagaacacc acaaggcta aagaagccc tccgtatgc 
      aataccgct acctggact tacgttacc ccctaatgc 
      agcgcaatt tacgcgtcc gttccgaaa tgctcatga 
      aaatgcgcg aatttctcg taaaccaca gcggttttg 
      tcgatgtcg agacgccaa ctctgttca aggccacgc 
      ctggggggg cgcaagaat tcatcgtgc caacgcgat 
      tcccaggcc agttctatt ccctagtcc agagccccc 
      aacaattca aacaaatgc taatggcgg gggctgttg 
      acaggtact ttcaaatcg cacggtgtt accgagacg 
      agggggcgc ggcctgacc gccagcccg agttcacgc 
      aactcgata tcgaaatgt ctttcactg acttggaca 
      acatcttgc aactagttg aggagttgt tggggcacg 
      cgtggcccc catttttgg gacctttgc ccaaaaaat 
      tccaaagga tggggttcg gggaggcta tggaattgt 
      acgggtcgg atcagcccg ataccaggt tcgggttta 
      agttggaaa attgtaccg agcttttga aactgaacg 
      aaaaattca ttaattcgg acgattttg gggcttatt 
      gcattgtct tcccaaaag aatacgcca atttgaata 
      agggagtga aggaaaatt tggaaaaaa ttagcaaac 
      agtttcccc ttgttaagt tcgttcaaa ctaaagtag 
      cgacacctg gtgaattgg cgcccaaac ttgggaaat 
      tgttgggcg ataacgtgg cggcaaaat tagccaatc 
      acgtgagcg acgattgtg ttttgtttt taggtttcg 
      ggaataaaa aacaaactc aaagcttga tgggaaaag 
      ttaggcttg aatatgcca atcatttgg aacaactag 
      gtgtgagtg tgcgtgcaa aaggcatgc acttgttgt 
      gggtggtgg acatgccgt tattcgaac cgggggaaa 
      gcccgggaa gtcttcaat cggcacatc acccattca 
      cagcgccga atcctgatg atttactcc tattggata 
      cggcgcctt tgcaggtgc ggtctttgt cattcgatt 
      tggttttga acgggaatg agatagggg gaggttcag 
      tacgtattc atgaccccg atcttcaag aaaaaattt 
      tccggatgt taaacattg ataaaaatt cgatgcaac 
      atattatcg atatgttat catcagggt gtccgcccc 
      atgggggca ttgcgctgg gactcgatc gtctcatgt 
      cgaaaattt taaatacgg cgagtattc gtgatgtga 
      tcgcgtttc ccaaaacat tcgaaggtc gggatccgt 
      tgagtgggg cgccgtcag ctgtttccg acgccgact 
      tgaagctct accatgtca agagtgttg ttgagtgaa 
      cagtatttt acatttttt acgaataaa tggtgcttt 
      ttaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY