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Gene information
General information
TC014736 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 14290429..14292750
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014736
    iBeetle screen iB_10123

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014736 RA
      MGIRGLTTF IANRSSQYL ESYELHDTY LVVDGNSIA 
      CQLYKWHCK CNDCFGGDY DKYAAVIKN FFQLLSDCN 
      ITPLVVFDG AYEKRKLKT VYSRMRNKI SASKDLNSV 
      TEGRISVFP LFLRELFVD IVTTLNIKC LRCDFEGDS 
      ESAELARTL GCPIVSFDS DFFIFDVLY IPFSTMQLR 
      LRKRKNVNY LVCEVYRIE KFLNSFGGL DKINLPLLA 
      ALLGNDYIK KSAFSVFYR QLKMHKTKQ NEQQTQIKS 
      VIVWLKNET PESALRKIL GRYKMNHRR TLARRIEKA 
      IGAYQSGES HICSYFNIE KVGDKRGKI IVPKPEDVE 
      DLGQGELEE GEIEDEMSS SEELTPDEP EIEESIPVL 
      KSIIPNFFR ENYKKCQYP PSFMDMIMS STYYFIPLI 
      EDYSLEPCH KISWEIVAA IYKILTSPK TRPLFCVMR 
      NEFSNIKRS PVPAYDREV PKLQELEHM TQQNAQQVL 
      LQILKIDKS FERFPKSWQ LFLIAINYW VTNTQKIDL 
      PHVYSLILC AIMLNHVDT KVGFYRSTK SFLSKYNSH 
      NEPAKPSTS TKNIPEALS AIDLSNSVS CAKTLIDYF 
      QMDAKMRCY VKLFDRGIV HDFAQLQSC LLHIKYLNS 
      LLNFPFPNF TISEFYDGT FLYNMTANL RKRTDLKGY 
      MGILLGKCP QILDAFELM AEEIENRVV FNAGRHEPP 
      RKRKKKKKA ISHLPETEL FSDVSDEEY VFDSNNKFS 
      LLSFVNK
    CDS sequence
    • >TC014736 RA
      atgggtatt cggggcttg acaacattt atagccaac 
      agatcgagt cagtacctc gagagttac gaactgcac 
      gatacttat ttggtggtc gatggtaac agcattgct 
      tgccagtta tacaaatgg cactgcaaa tgcaacgac 
      tgttttggg ggcgattac gacaaatac gcggccgtt 
      atcaagaat ttctttcaa ttgttgtcc gactgtaac 
      atcacccct ctagttgtg ttcgatgga gcgtacgaa 
      aaacgcaaa ttaaagaca gtatacagc agaatgagg 
      aacaaaatc agtgcctct aaagacctg aacagtgtg 
      accgagggc cgtatttcg gtgtttccc ctatttttg 
      cgcgaactt ttcgtcgac atcgtcacc actttgaac 
      ataaagtgc ctgagatgt gactttgag ggtgacagc 
      gagtcagct gagctcgcg cggactttg gggtgccct 
      attgttagt ttcgactcg gattttttt attttcgac 
      gtgttgtac atcccgttc tccaccatg cagttacgg 
      ttacgaaag cgcaaaaat gtgaactac ttagtgtgt 
      gaggtgtac agaatcgaa aaattttta aattcgttt 
      ggaggcttg gataaaatt aatttgccg ctacttgcc 
      gccttgttg gggaacgat tacataaag aaaagtgcc 
      ttttcggtg ttttatcgt cagttgaaa atgcacaaa 
      acgaaacag aatgagcag caaactcag attaaatcg 
      gtaattgtt tggctcaaa aacgaaacg ccggaatca 
      gcccttcgg aaaattttg gggcgatat aaaatgaat 
      caccggagg actttggca cgtagaatt gagaaggca 
      attggggcg tatcaaagc ggcgaaagc cacatttgt 
      tcgtacttc aatattgaa aaagttggt gataaacgt 
      gggaaaata attgtgccc aagcctgaa gatgttgaa 
      gatttgggg caaggagag cttgaagaa ggagaaatt 
      gaggatgaa atgtccagt tcggaagaa ctaacgccc 
      gacgagcca gaaattgaa gaatcaatc cctgtgtta 
      aaatcaatt attcccaac ttttttcgc gaaaattac 
      aaaaagtgt caatatccg ccctcgttc atggacatg 
      atcatgtcc agcacgtac tacttcatc cctctaatc 
      gaggattat tccctcgaa ccttgccat aaaatcagc 
      tgggaaata gttgcggca atttacaaa attctaaca 
      tccccaaaa acacgacct ttattttgc gtaatgcgc 
      aacgagttt tcaaacatt aaaagatcc cccgtccca 
      gcgtacgac cgcgaagtg ccaaaacta caagaactt 
      gaacatatg acccaacaa aacgcccag caagtgtta 
      ctacaaatt ttaaaaatc gacaaatcg ttcgaacgt 
      ttccccaag tcttggcaa cttttcctg atcgctatt 
      aattactgg gtgacgaac acgcaaaaa atcgacctg 
      ccccacgtc tactcgctc attctttgc gcaatcatg 
      ttaaaccac gtcgacacc aaagtcggt ttctataga 
      tccacgaaa tcatttttg tccaaatat aactcgcat 
      aacgaaccc gcaaaaccc tcaacttcc acgaaaaac 
      ataccagag gccttgagc gcaatcgat ttgagtaac 
      agtgtctcg tgtgcgaaa accttgatt gattacttc 
      caaatggac gcgaaaatg cgctgttac gttaaatta 
      tttgacagg ggcattgtc cacgatttt gcacaactg 
      cagagctgt ctgctgcat atcaagtac ttgaactcg 
      ctgttgaac tttccgttt ccgaatttc acaatttcg 
      gagttttac gacgggacg tttctctac aacatgaca 
      gcgaatctg cggaaaaga acggacttg aaagggtat 
      atgggaatt ctgctgggg aagtgcccc caaattttg 
      gatgcgttc gaactgatg gctgaggaa attgaaaat 
      cgggttgtc ttcaacgcg gggaggcat gagccccca 
      aggaagagg aagaagaag aagaaagcc attagtcat 
      ctgcccgaa actgagctt ttcagcgat gtctcggat 
      gaggaatac gttttcgat tcaaataac aagttctct 
      ttgttatct tttgtaaat aaataa
    mRNA sequence
    • >TC014736 RA
      tcattattc attagggag gttagctat ctgtcaact 
      tgacaatgg cacataacc tgtaccagt aaacgtgaa 
      actcaaaat gggtattcg gggcttgac aacatttat 
      agccaacag atcgagtca gtacctcga gagttacga 
      actgcacga tacttattt ggtggtcga tggtaacag 
      cattgcttg ccagttata caaatggca ctgcaaatg 
      caacgactg ttttggggg cgattacga caaatacgc 
      ggccgttat caagaattt ctttcaatt gttgtccga 
      ctgtaacat cacccctct agttgtgtt cgatggagc 
      gtacgaaaa acgcaaatt aaagacagt atacagcag 
      aatgaggaa caaaatcag tgcctctaa agacctgaa 
      cagtgtgac cgagggccg tatttcggt gtttcccct 
      atttttgcg cgaactttt cgtcgacat cgtcaccac 
      tttgaacat aaagtgcct gagatgtga ctttgaggg 
      tgacagcga gtcagctga gctcgcgcg gactttggg 
      gtgccctat tgttagttt cgactcgga tttttttat 
      tttcgacgt gttgtacat cccgttctc caccatgca 
      gttacggtt acgaaagcg caaaaatgt gaactactt 
      agtgtgtga ggtgtacag aatcgaaaa atttttaaa 
      ttcgtttgg aggcttgga taaaattaa tttgccgct 
      acttgccgc cttgttggg gaacgatta cataaagaa 
      aagtgcctt ttcggtgtt ttatcgtca gttgaaaat 
      gcacaaaac gaaacagaa tgagcagca aactcagat 
      taaatcggt aattgtttg gctcaaaaa cgaaacgcc 
      ggaatcagc ccttcggaa aattttggg gcgatataa 
      aatgaatca ccggaggac tttggcacg tagaattga 
      gaaggcaat tggggcgta tcaaagcgg cgaaagcca 
      catttgttc gtacttcaa tattgaaaa agttggtga 
      taaacgtgg gaaaataat tgtgcccaa gcctgaaga 
      tgttgaaga tttggggca aggagagct tgaagaagg 
      agaaattga ggatgaaat gtccagttc ggaagaact 
      aacgcccga cgagccaga aattgaaga atcaatccc 
      tgtgttaaa atcaattat tcccaactt ttttcgcga 
      aaattacaa aaagtgtca atatccgcc ctcgttcat 
      ggacatgat catgtccag cacgtacta cttcatccc 
      tctaatcga ggattattc cctcgaacc ttgccataa 
      aatcagctg ggaaatagt tgcggcaat ttacaaaat 
      tctaacatc cccaaaaac acgaccttt attttgcgt 
      aatgcgcaa cgagttttc aaacattaa aagatcccc 
      cgtcccagc gtacgaccg cgaagtgcc aaaactaca 
      agaacttga acatatgac ccaacaaaa cgcccagca 
      agtgttact acaaatttt aaaaatcga caaatcgtt 
      cgaacgttt ccccaagtc ttggcaact tttcctgat 
      cgctattaa ttactgggt gacgaacac gcaaaaaat 
      cgacctgcc ccacgtcta ctcgctcat tctttgcgc 
      aatcatgtt aaaccacgt cgacaccaa agtcggttt 
      ctatagatc cacgaaatc atttttgtc caaatataa 
      ctcgcataa cgaacccgc aaaaccctc aacttccac 
      gaaaaacat accagaggc cttgagcgc aatcgattt 
      gagtaacag tgtctcgtg tgcgaaaac cttgattga 
      ttacttcca aatggacgc gaaaatgcg ctgttacgt 
      taaattatt tgacagggg cattgtcca cgattttgc 
      acaactgca gagctgtct gctgcatat caagtactt 
      gaactcgct gttgaactt tccgtttcc gaatttcac 
      aatttcgga gttttacga cgggacgtt tctctacaa 
      catgacagc gaatctgcg gaaaagaac ggacttgaa 
      agggtatat gggaattct gctggggaa gtgccccca 
      aattttgga tgcgttcga actgatggc tgaggaaat 
      tgaaaatcg ggttgtctt caacgcggg gaggcatga 
      gcccccaag gaagaggaa gaagaagaa gaaagccat 
      tagtcatct gcccgaaac tgagctttt cagcgatgt 
      ctcggatga ggaatacgt tttcgattc aaataacaa 
      gttctcttt gttatcttt tgtaaataa ataaagttt 
      ggttgattt tttatcctt taatttgcg taatattaa 
      tacacatag cacagttaa
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