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Gene information
General information
TC013613 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 3118774..3122036
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013613
    iBeetle screen iB_10121

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013613 RA
      MHLWIIPIG NIFLVCFLF HFSHEYSPR EYYVPWLDE 
      ETDSPRWVK LPQRRNVES QQSAENTRH FAPDTINRI 
      TETLGAINT VGRYLVNMT RGATDTATL SDEVPSAIY 
      TISKNVLGR NVTDSIAPL VREALPVVI DNKNDEEEE 
      DEDPKSCTT PEGLKGYCD DLSDCPQLL LNLGNLRQS 
      LCFKSLFVP GVCCPRTGN VESVTLKPV QVTTIRTQK 
      LKPVPVVTP PPTTKPPPV EIPNLGLNP LGNIVDPSD 
      CGQPESAKY RVVGGEEAL PGRWPWMAA IFLHGSRRT 
      EFWCGGSLI TAKHVLTAA HCTRDSRQR PFAAKQFTV 
      RLGDIDLKR NDEPSSPIT FKVSEIRAH PQFSRVGFY 
      NDIAVLVLD KPARKSKYV IPLCLPPPE LRNEKFAGR 
      KTTVVGWGT TFYGGKEST VQRQAVLPI WRNEDCNQA 
      YFQPITDNF ICAGYSEGG TDACQGDSG GPLMIHWDT 
      RWIQVGVVS FGNKCGEPG YPGVYTRIT NYLEWIKEN 
      TKT
    CDS sequence
    • >TC013613 RA
      atgcattta tggattata ccaatcgga aacattttc 
      cttgtctgt tttttgttc catttctct catgaatac 
      agtccacga gagtattac gtgccgtgg ctagacgaa 
      gagaccgac agcccacgg tgggtaaaa ctgccacag 
      aggcgcaat gtcgagagc cagcaaagt gccgaaaat 
      acgcgacac tttgctccc gacaccata aacaggata 
      accgagacg ttgggtgcg ataaacaca gtagggcga 
      tatttagtg aatatgacc aggggtgcc actgacact 
      gctacctta tcagacgaa gtaccaagt gccatttat 
      acgattagc aagaacgtg ttaggtaga aacgtgact 
      gattccatt gctccccta gtcagagag gcactacct 
      gtcgtcatt gacaacaaa aacgacgaa gaagaggaa 
      gacgaagac cccaaaagc tgcacgact ccggaagga 
      ctaaaggga tattgcgac gatctgagt gactgtccc 
      caattattg ctaaattta ggaaacctc cgacaatcc 
      ttgtgtttc aaaagcctc ttcgtgcct ggcgtttgt 
      tgtcccagg accggtaat gttgaatcg gttacgtta 
      aaaccggtc caggtcaca acaatacgg acacagaaa 
      ctcaaacct gtgccggtt gtcacaccg cctccaaca 
      accaaaccg ccaccagtc gaaattccg aacttgggt 
      ctaaaccct ctgggcaat atcgtggac ccgtcagat 
      tgtggtcag cccgagtcg gctaaatac cgggtggtc 
      ggaggcgag gaggccttg ccggggcgg tggccctgg 
      atggcggca attttcctg cacgggtct cggcgtacg 
      gaattttgg tgcggcggc tctctaatt acggcaaaa 
      catgttcta acggccgct cattgcacg agagattcg 
      cgccagcga ccatttgca gcaaaacaa ttcaccgta 
      agattgggc gatattgat ttgaaaaga aacgatgag 
      ccctcgagt cctattacg tttaaagta tcagaaata 
      agagcgcat cctcaattt agtcgtgtt ggcttttat 
      aatgatatt gcagtattg gttttggat aaaccggca 
      aggaaatcg aaatatgtt attccactt tgcttgccg 
      cctcccgaa ttacggaat gagaaattc gcagggagg 
      aaaaccacg gtcgtgggc tggggcacc actttctac 
      ggggggaaa gaaagcacg gtgcagagg caagccgtc 
      ttgccgatt tggaggaac gaggattgc aatcaggcc 
      tattttcag ccaatcacc gataatttt atctgtgca 
      ggctactcg gaaggcggg acggacgct tgccaggga 
      gattcaggt gggcccttg atgatccac tgggacacg 
      cgctggatt caagtcgga gtggtgtcc ttcggaaac 
      aagtgcggg gaacccggc tatcctgga gtctacacc 
      aggatcacc aattacctc gaatggatt aaggaaaat 
      accaagaca tag
    mRNA sequence
    • >TC013613 RA
      agtaaattt gggtcaaac gttgacttt cagttaagt 
      gttgtgcat ggtgcgcta gtgctaaca tactcaatg 
      cacttcagg atgcattta tggattata ccaatcgga 
      aacattttc cttgtctgt tttttgttc catttctct 
      catgaatac agtccacga gagtattac gtgccgtgg 
      ctagacgaa gagaccgac agcccacgg tgggtaaaa 
      ctgccacag aggcgcaat gtcgagagc cagcaaagt 
      gccgaaaat acgcgacac tttgctccc gacaccata 
      aacaggata accgagacg ttgggtgcg ataaacaca 
      gtagggcga tatttagtg aatatgacc aggggtgcc 
      actgacact gctacctta tcagacgaa gtaccaagt 
      gccatttat acgattagc aagaacgtg ttaggtaga 
      aacgtgact gattccatt gctccccta gtcagagag 
      gcactacct gtcgtcatt gacaacaaa aacgacgaa 
      gaagaggaa gacgaagac cccaaaagc tgcacgact 
      ccggaagga ctaaaggga tattgcgac gatctgagt 
      gactgtccc caattattg ctaaattta ggaaacctc 
      cgacaatcc ttgtgtttc aaaagcctc ttcgtgcct 
      ggcgtttgt tgtcccagg accggtaat gttgaatcg 
      gttacgtta aaaccggtc caggtcaca acaatacgg 
      acacagaaa ctcaaacct gtgccggtt gtcacaccg 
      cctccaaca accaaaccg ccaccagtc gaaattccg 
      aacttgggt ctaaaccct ctgggcaat atcgtggac 
      ccgtcagat tgtggtcag cccgagtcg gctaaatac 
      cgggtggtc ggaggcgag gaggccttg ccggggcgg 
      tggccctgg atggcggca attttcctg cacgggtct 
      cggcgtacg gaattttgg tgcggcggc tctctaatt 
      acggcaaaa catgttcta acggccgct cattgcacg 
      agagattcg cgccagcga ccatttgca gcaaaacaa 
      ttcaccgta agattgggc gatattgat ttgaaaaga 
      aacgatgag ccctcgagt cctattacg tttaaagta 
      tcagaaata agagcgcat cctcaattt agtcgtgtt 
      ggcttttat aatgatatt gcagtattg gttttggat 
      aaaccggca aggaaatcg aaatatgtt attccactt 
      tgcttgccg cctcccgaa ttacggaat gagaaattc 
      gcagggagg aaaaccacg gtcgtgggc tggggcacc 
      actttctac ggggggaaa gaaagcacg gtgcagagg 
      caagccgtc ttgccgatt tggaggaac gaggattgc 
      aatcaggcc tattttcag ccaatcacc gataatttt 
      atctgtgca ggctactcg gaaggcggg acggacgct 
      tgccaggga gattcaggt gggcccttg atgatccac 
      tgggacacg cgctggatt caagtcgga gtggtgtcc 
      ttcggaaac aagtgcggg gaacccggc tatcctgga 
      gtctacacc aggatcacc aattacctc gaatggatt 
      aaggaaaat accaagaca tagacgcta tgaaactta 
      ctcgctcaa ttagcgcgt gatgtcaac agtgaccaa 
      gtttaataa ttacttaat aactgc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY