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Gene information
General information
TC014707 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 13660301..13664180
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014707
    iBeetle screen iB_10118

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014707 RA
      MSAPPPPPC DWQTTRKLV KERGQYLLE TGMWSDCRF 
      IVGTDPNQQ VLEGHKLFL AMSSPVFEA MFFGGMAEK 
      DPIAILDVQ PDAFKALLE YIYTDKINL TSFDQACEL 
      CYGAKKYML PHLVEECTK YLWSDLYPK NACRAYEFA 
      KLFEEPILM EKCMLIICN QTQEVLSES SFDDVELST 
      ILTVFDQDE LNISSELEL FSAISRYAA RHNQSSGAK 
      VPRLDGIGN PSSEGSNGN HPTIRDAIM KIRFLTLTP 
      QQFAEVPAS CNLLTESEK FAILMNICS PSATTPMPP 
      GFSTSRIAR RRKQSIRNE CIFMGGASS MDTSRTSMI 
      KFSLSANEG GNESPKKLY CCRQLLRIT ECMNTSVLD 
      CAVTFTCDR NVCVYGIQV PTQIPVEDD RQQSYSYPE 
      LLYAHLLDA DGSRLTYTH FTTRVAFRS LIEISFNRP 
      VYIQRNKVY KVGVVLNKI GRYPIGTYP SQSTCNGVV 
      FTFSQGQSG DSVRDGLVR SIIFSIPSS NHNSINNPG 
      ISGIPGDHN KMSNII
    CDS sequence
    • >TC014707 RA
      atgagtgcc cctccgcca ccgccttgc gactggcaa 
      accacccga aaactggtg aaagaaaga gggcagtac 
      ctccttgaa accgggatg tggagcgac tgcagattt 
      attgtgggc acagacccc aatcagcag gtcctggaa 
      ggacacaag cttttttta gccatgtct agtccagtt 
      ttcgaagct atgtttttt ggggggatg gctgagaaa 
      gacccaatt gcaatactc gacgtacaa cctgacgca 
      tttaaagct ttattagag tacatctac accgataaa 
      atcaattta acgtctttt gaccaagct tgcgagctg 
      tgctatggc gctaaaaag tacatgttg ccccaccta 
      gtggaagag tgcaccaag tatttgtgg tcggatttg 
      tacccaaag aacgcctgt cgcgcctac gaattcgcc 
      aagttgttt gaggagccg attttgatg gaaaaatgc 
      atgcttata atctgcaat cagacgcaa gaagttctc 
      tcagagagc agctttgac gatgttgag cttagcaca 
      atccttaca gttttcgac caagacgaa ctaaacatc 
      agctcagaa ctagaactg ttttctgcc atttctcgt 
      tacgcagca aggcacaat caaagttca ggtgcgaaa 
      gtacctaga ctagatggt atagggaac ccgtctagt 
      gagggcagt aatggcaat catcccaca ataagggat 
      gccattatg aagatcagg ttcctcact ttgacacca 
      caacagttt gctgaagtc ccagcgagt tgtaatctc 
      ctaacagag tcggaaaaa ttcgcgatt ctgatgaat 
      atttgctca cccagcgcc accacgccg atgcctcca 
      gggttctca acgtctagg atcgccagg aggaggaaa 
      caaagcatc aggaatgag tgcatattt atgggtgga 
      gcttcctcg atggataca tctcggact tcgatgatt 
      aaatttagt ttatcagcc aacgagggc ggcaatgaa 
      tccccaaaa aaactctat tgttgccgt caattactg 
      cgaataaca gaatgcatg aatacaagc gttctcgat 
      tgcgcagtt acatttact tgcgaccga aatgtttgt 
      gtgtatgga attcaggtg cccacacaa ataccggtg 
      gaggacgac cgccagcag tcgtactcc tatccggag 
      ctgctctac gcccatctg ctggatgcc gacggaagt 
      cgcctgact tacacgcac ttcaccacg agggtggct 
      ttcaggtct ctgattgaa atctccttc aacagaccc 
      gtttacata cagcggaat aaggtgtac aaagttgga 
      gtggttttg aataaaatt gggcgatat cctattggc 
      acatatcca agtcagtcc acttgcaat ggagtcgtt 
      tttactttt agccaaggc cagagtggc gactccgtc 
      agagatggg ctagtgcgt tcaataatt ttctcaata 
      ccctcgtcg aatcacaac agtattaat aacccggga 
      atatctggt atacccggt gaccataac aaaatgtcc 
      aacatcatc taa
    mRNA sequence
    • >TC014707 RA
      gaatacagc gagagtaaa agtccccaa acgcagtgt 
      ttgccatga aaggtcaaa gaaacaaca aaaaattaa 
      caacaatcg ttttgctgg tattaaaaa tatccaaat 
      aattaattg ttgaattaa gccgccgtg tatattgtt 
      ataaacagg ttgaacgat tgtgactgc attgagtgg 
      gaagtcaag catcaaaga tgagtgccc ctccgccac 
      cgccttgcg actggcaaa ccacccgaa aactggtga 
      aagaaagag ggcagtacc tccttgaaa ccgggatgt 
      ggagcgact gcagattta ttgtgggca cagacccca 
      atcagcagg tcctggaag gacacaagc tttttttag 
      ccatgtcta gtccagttt tcgaagcta tgttttttg 
      gggggatgg ctgagaaag acccaattg caatactcg 
      acgtacaac ctgacgcat ttaaagctt tattagagt 
      acatctaca ccgataaaa tcaatttaa cgtcttttg 
      accaagctt gcgagctgt gctatggcg ctaaaaagt 
      acatgttgc cccacctag tggaagagt gcaccaagt 
      atttgtggt cggatttgt acccaaaga acgcctgtc 
      gcgcctacg aattcgcca agttgtttg aggagccga 
      ttttgatgg aaaaatgca tgcttataa tctgcaatc 
      agacgcaag aagttctct cagagagca gctttgacg 
      atgttgagc ttagcacaa tccttacag ttttcgacc 
      aagacgaac taaacatca gctcagaac tagaactgt 
      tttctgcca tttctcgtt acgcagcaa ggcacaatc 
      aaagttcag gtgcgaaag tacctagac tagatggta 
      tagggaacc cgtctagtg agggcagta atggcaatc 
      atcccacaa taagggatg ccattatga agatcaggt 
      tcctcactt tgacaccac aacagtttg ctgaagtcc 
      cagcgagtt gtaatctcc taacagagt cggaaaaat 
      tcgcgattc tgatgaata tttgctcac ccagcgcca 
      ccacgccga tgcctccag ggttctcaa cgtctagga 
      tcgccagga ggaggaaac aaagcatca ggaatgagt 
      gcatattta tgggtggag cttcctcga tggatacat 
      ctcggactt cgatgatta aatttagtt tatcagcca 
      acgagggcg gcaatgaat ccccaaaaa aactctatt 
      gttgccgtc aattactgc gaataacag aatgcatga 
      atacaagcg ttctcgatt gcgcagtta catttactt 
      gcgaccgaa atgtttgtg tgtatggaa ttcaggtgc 
      ccacacaaa taccggtgg aggacgacc gccagcagt 
      cgtactcct atccggagc tgctctacg cccatctgc 
      tggatgccg acggaagtc gcctgactt acacgcact 
      tcaccacga gggtggctt tcaggtctc tgattgaaa 
      tctccttca acagacccg tttacatac agcggaata 
      aggtgtaca aagttggag tggttttga ataaaattg 
      ggcgatatc ctattggca catatccaa gtcagtcca 
      cttgcaatg gagtcgttt ttactttta gccaaggcc 
      agagtggcg actccgtca gagatgggc tagtgcgtt 
      caataattt tctcaatac cctcgtcga atcacaaca 
      gtattaata acccgggaa tatctggta tacccggtg 
      accataaca aaatgtcca acatcatct aatgcccat 
      gactcgatt ctagatgta tttgtgtat taaatgtat 
      gtttatttt taaatgaaa taaataaat ggtgattca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY