Status
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Gene information
General information
TC014612 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12364804..12367652
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014612
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10105

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: number 2nd lateral oviduct: - Number of animals showing the phenotype: 1

    ovariole degenerated - Number of animals showing the phenotype: 4

    yolk deposition degenerated - Number of animals showing the phenotype: 4

    previtellogenic egg chamber size decreased - Number of animals showing the phenotype: 2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014612 RA
      MPLFGKSQK SPTDIVKAL KEAVNALEK GEKKAEKAQ 
      EEVSKNLVL IKNMLYGTS DAEPQTDII VAQLAQELY 
      NSNLLLLLI QNLNRIDFE GKKDVAQVF NNILRRQIG 
      TRSPTVEYI CTKPEILFT LMSGYELQE IALNCGTML 
      RECARYEAL AKIMLYSDD FYNFFRYVE VSTFDIASD 
      AFSTFKELL TRHKILCAD FLEVNYDKV FSHYQRLLN 
      SENYVTRRQ SLKLLGELL LDRHNFTVM TRYISNPDN 
      LKLMMNMLK ERSRNIQFE AFHVFKVFV ANPNKPKPI 
      LDILLRNQD KLVDFLMKF HTDRAEDEQ FNDEKAYLI 
      RQIKELRPM AGH
    CDS sequence
    • >TC014612 RA
      atgcctctg tttggaaaa tcgcaaaag agccccacc 
      gatatcgtg aaagccttg aaggaggcg gtgaatgcg 
      ctggaaaaa ggcgagaag aaggcggaa aaggcgcaa 
      gaggaagtc agcaagaac ctggtgctg atcaagaac 
      atgttgtat gggacgtcc gatgcggag ccccagacg 
      gacataata gtggcccag ctggctcag gagctgtac 
      aacagcaac ttgctgctg ctcctgatc cagaatctg 
      aatcggatt gattttgag gggaaaaag gacgtggcc 
      caggtgttc aataacatt ctgcgaagg cagattggc 
      acgcggtct ccgaccgtg gaatacatc tgtaccaag 
      cctgagatt ttgttcacg ctgatgtca gggtacgag 
      ttgcaggaa atcgccctg aattgcggg acgatgttg 
      cgcgagtgc gcccgctat gaggccttg gccaaaatc 
      atgctttat tccgacgat ttctacaat ttttttaga 
      tacgtcgaa gtgtcgact tttgatatc gcctcagat 
      gcgttttct acgtttaag gaattgttg acgcgtcac 
      aaaatcttg tgtgcggac tttttggaa gttaattat 
      gacaaagtt ttcagccat tatcaacga ctcttaaat 
      tccgaaaat tacgtcact agacgtcaa agtttgaaa 
      ttgctcggc gaattactt ctggataga cataatttc 
      actgtgatg acgaggtat atatccaat ccagacaat 
      cttaaactt atgatgaat atgcttaag gaacgatct 
      aggaacatc caatttgaa gccttccat gtatttaag 
      gtgtttgtg gcaaatccc aacaaaccc aagccaatt 
      ttggacatt ttgctccgc aatcaagac aaattagta 
      gacttcttg atgaaattc cacacggac cgggcggaa 
      gacgaacag tttaacgac gaaaaagcg tacctcatt 
      aggcaaatt aaagaacta aggccgatg gccgggcat 
      taa
    mRNA sequence
    • >TC014612 RA
      cagtccatc ccttatgac tgtttcgtt ctgtcaaat 
      gtgtctaaa agtggtctg aatttctca aaaataaac 
      acttttgtg atgagtagt gtaaagttt tgctagtcc 
      ccggtgaag gcacgctcg acaacctca aaaaccgcc 
      tcttgacct caaaaaacc cgcgaaatc tgtcactgt 
      tcacccaaa aaatgcctc tgtttggaa aatcgcaaa 
      agagcccca ccgatatcg tgaaagcct tgaaggagg 
      cggtgaatg cgctggaaa aaggcgaga agaaggcgg 
      aaaaggcgc aagaggaag tcagcaaga acctggtgc 
      tgatcaaga acatgttgt atgggacgt ccgatgcgg 
      agccccaga cggacataa tagtggccc agctggctc 
      aggagctgt acaacagca acttgctgc tgctcctga 
      tccagaatc tgaatcgga ttgattttg aggggaaaa 
      aggacgtgg cccaggtgt tcaataaca ttctgcgaa 
      ggcagattg gcacgcggt ctccgaccg tggaataca 
      tctgtacca agcctgaga ttttgttca cgctgatgt 
      cagggtacg agttgcagg aaatcgccc tgaattgcg 
      ggacgatgt tgcgcgagt gcgcccgct atgaggcct 
      tggccaaaa tcatgcttt attccgacg atttctaca 
      attttttta gatacgtcg aagtgtcga cttttgata 
      tcgcctcag atgcgtttt ctacgttta aggaattgt 
      tgacgcgtc acaaaatct tgtgtgcgg actttttgg 
      aagttaatt atgacaaag ttttcagcc attatcaac 
      gactcttaa attccgaaa attacgtca ctagacgtc 
      aaagtttga aattgctcg gcgaattac ttctggata 
      gacataatt tcactgtga tgacgaggt atatatcca 
      atccagaca atcttaaac ttatgatga atatgctta 
      aggaacgat ctaggaaca tccaatttg aagccttcc 
      atgtattta aggtgtttg tggcaaatc ccaacaaac 
      ccaagccaa ttttggaca ttttgctcc gcaatcaag 
      acaaattag tagacttct tgatgaaat tccacacgg 
      accgggcgg aagacgaac agtttaacg acgaaaaag 
      cgtacctca ttaggcaaa ttaaagaac taaggccga 
      tggccgggc attaacaat tttcctatt aacattttt 
      attaattat tattgttaa atatatatt tttaatttc 
      agccgttac caagattta agccccaag gactcccgt 
      atcatttaa tttcattga cgtccattt tatttgatt 
      cttttacct gagctgagt ttcgcgctc aagcgataa 
      taaatcgag tgatttcgt ttgtagttg agtttaggg 
      tcggtaaaa gtataacta agtgtattt ataataata 
      tatttatac ggaggagat gttaagtgt ttagtttgg 
      tagggagca gaacacgaa ctgactttg cctaaatca 
      aacctgatg ttctgccgg ttgtcgttt gtttcgttc 
      cccttgccc ccttttgtt ggcttgtag caataattt 
      ccgacttaa gtgtacttt gtttttata aaaataagt 
      ctagtggtt agttagttt agtaatgaa ttctgtaaa 
      tctattaat tcaattata aaaacatag tggggtgcc 
      tataaatgc ttatgggta gttttaaac caattaatc 
      ttattagaa attaaatga ccatccatt ggggagtgg 
      gccgatttt cgccggttt tcgcgtggg aaaaccggg 
      gaagtggcc aacggcact tgtgtaaat taatggagg 
      gctgtaatt actaatagg atctattct tagcatact 
      taaacttaa aaaatgaaa atgatagga tacctaata 
      caattctaa atcttattg aaattgaat agttttata 
      aaagaattt ctattcgag gataaaatt actaaggtg 
      gattgttgt ttcgttcca ggaggtgcg ttagcgtta 
      catttaggc acttgttca aatgttcaa acacgagtt 
      gcgaaagcg aaattctct tttttctac acgttttag 
      ttatgtatg ttattgtat ttattttaa attatatga 
      tttacacag aaattggaa atacattct tttagatga 
      t
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