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Gene information
General information
TC014590 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12155148..12157653
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014590
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10101

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014590 RA
      MAGTLTKFS KSLNAVATK NGLLQGRNY GTQRVVAKN 
      PVVEMDGDE MTRIIWEKI KERLIFPYV KLESLYFDL 
      GLPHRDQTN DQVTIDAAN AILKHNVGI KCATITPDE 
      QRVQEFNLK KMWLSPNGT IRNILGGTV FREPILCKN 
      IPKLVPGWT KPIVIGRHA HGDQYKAKD FVVTKPGTV 
      ELVYTSDDG KVEKYELFK YKGGGVALG MYNTDESIR 
      AFAHSSFQV ALNKGWPLY LSTKNTILK KYDGRFKDI 
      FQEIYEKEY KSQYEAKKI WYEHRLIDD MVAQGLKSS 
      GGFVWACKN YDGDVQSDI VAQGYGSLG MMTSVLMCP 
      DGKTIESEA AHGTVTRHY REHQKGNPT STNPIASIF 
      AWTRGLEHR AKLDGTPDL AKFAQTLEK ACVDCVESG 
      KMTKDLAAS IHGLPNVKP GMYLNTQDF LEAIAEELE 
      RQMK
    CDS sequence
    • >TC014590 RA
      atggccggt actttgacc aaattttcg aaaagcttg 
      aacgctgtt gccacaaaa aatgggctg ctgcaaggg 
      cgcaactat gggacccag cgcgtggtg gccaagaac 
      cccgtggtg gagatggac ggcgacgaa atgacccga 
      atcatctgg gagaaaatc aaagaacgc ctaatcttc 
      ccctacgtt aaactcgag tctctttac ttcgacttg 
      gggcttccc caccgtgac caaaccaac gaccaagtg 
      accatcgac gccgccaac gccatcctc aagcacaac 
      gtggggatc aaatgcgcc accatcacc cccgacgag 
      cagcgagtc caagaattc aacttgaaa aaaatgtgg 
      ctttccccc aacggcacc atccgaaat attttaggc 
      gggacggtt ttccgcgaa cccatttta tgcaaaaac 
      atccccaag ctggtaccc ggctggacc aagcccatc 
      gtcatcggg cgtcacgcc catggcgac cagtacaaa 
      gccaaagat ttcgttgta acgaaacca ggaaccgtc 
      gaacttgtc tacacttcg gacgatggt aaagtcgaa 
      aagtacgag cttttcaag tacaaaggg ggcggcgtc 
      gccctgggg atgtacaac acggatgag agcatccgc 
      gccttcgcc cattcgtcc ttccaggtg gccttaaac 
      aaaggctgg ccgctgtat ctctccacc aaaaacacg 
      attttgaag aagtacgac gggaggttc aaagacatc 
      ttccaggag atttacgaa aaagagtac aagtctcaa 
      tacgaggcg aagaaaatc tggtatgag caccgcttg 
      attgacgac atggtggcc caagggctg aaatcgtca 
      ggtggtttc gtctgggct tgcaaaaac tacgatgga 
      gacgtccag tcggacata gtcgcccaa gggtacggt 
      tccctgggg atgatgacc tcggtgctg atgtgtccc 
      gacgggaag accatcgag tcggaggcc gcccatggg 
      acggtcacg aggcactac cgcgagcac cagaagggg 
      aatcccacg tccactaac cccatagcg tccattttt 
      gcctggact aggggcctg gaacataga gccaagctt 
      gatgggacg cccgatttg gccaagttt gcgcaaact 
      ttggagaag gcgtgcgtt gattgcgtc gagtcaggg 
      aaaatgacc aaggatttg gctgcgtcc attcatggg 
      ctgcccaat gtcaagccg gggatgtat ttgaatact 
      caagacttt ttagaggcg atcgcagag gagttggaa 
      aggcagatg aagtga
    mRNA sequence
    • >TC014590 RA
      atgctcggc tgtttccgc cgcctactc cacaacctc 
      aacaagttg tttttcgat tttcagttt ctgagctga 
      aacagtcga gttggggtt acaatggcc ggtactttg 
      accaaattt tcgaaaagc ttgaacgct gttgccaca 
      aaaaatggg ctgctgcaa gggcgcaac tatgggacc 
      cagcgcgtg gtggccaag aaccccgtg gtggagatg 
      gacggcgac gaaatgacc cgaatcatc tgggagaaa 
      atcaaagaa cgcctaatc ttcccctac gttaaactc 
      gagtctctt tacttcgac ttggggctt ccccaccgt 
      gaccaaacc aacgaccaa gtgaccatc gacgccgcc 
      aacgccatc ctcaagcac aacgtgggg atcaaatgc 
      gccaccatc acccccgac gagcagcga gtccaagaa 
      ttcaacttg aaaaaaatg tggctttcc cccaacggc 
      accatccga aatatttta ggcgggacg gttttccgc 
      gaacccatt ttatgcaaa aacatcccc aagctggta 
      cccggctgg accaagccc atcgtcatc gggcgtcac 
      gcccatggc gaccagtac aaagccaaa gatttcgtt 
      gtaacgaaa ccaggaacc gtcgaactt gtctacact 
      tcggacgat ggtaaagtc gaaaagtac gagcttttc 
      aagtacaaa gggggcggc gtcgccctg gggatgtac 
      aacacggat gagagcatc cgcgccttc gcccattcg 
      tccttccag gtggcctta aacaaaggc tggccgctg 
      tatctctcc accaaaaac acgattttg aagaagtac 
      gacgggagg ttcaaagac atcttccag gagatttac 
      gaaaaagag tacaagtct caatacgag gcgaagaaa 
      atctggtat gagcaccgc ttgattgac gacatggtg 
      gcccaaggg ctgaaatcg tcaggtggt ttcgtctgg 
      gcttgcaaa aactacgat ggagacgtc cagtcggac 
      atagtcgcc caagggtac ggttccctg gggatgatg 
      acctcggtg ctgatgtgt cccgacggg aagaccatc 
      gagtcggag gccgcccat gggacggtc acgaggcac 
      taccgcgag caccagaag gggaatccc acgtccact 
      aaccccata gcgtccatt tttgcctgg actaggggc 
      ctggaacat agagccaag cttgatggg acgcccgat 
      ttggccaag tttgcgcaa actttggag aaggcgtgc 
      gttgattgc gtcgagtca gggaaaatg accaaggat 
      ttggctgcg tccattcat gggctgccc aatgtcaag 
      ccggggatg tatttgaat actcaagac tttttagag 
      gcgatcgca gaggagttg gaaaggcag atgaagtga 
      tcgactaaa cgaattttt tatgcaaat ttgtaaata 
      ttttttcaa ataaagctt ttacataaa c
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY