Status
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Gene information
General information
TC013215 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 12129822..12133468
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013215
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10100

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature random pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    abdomen shape irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013215 RA
      MTKGSYSVG DNDHQPSRR DTSYKSFIC VLLIFLFIC 
      LIILLAAIG WGSGHGRNN LEKTPKETS NQRDSQVEV 
      LERKRSQQS NNTFEKLIV AETTTFVNK IVNTIPEST 
      TEKLEKKIL RDKEWPITR PVEESTTKI FESVTTITT 
      PEATTEENV VTSTDSVVV TKLEKLDTT QVTLNVTKG 
      QGFVETTTS DYETTDEIS NQNVSTVTQ NLAKETEFS 
      ETTTLEYGT TEEISGQNV STVAQNVSK TAKLVDNTF 
      SETTTEMFT QNVTTEVSI MESEAITEP TPQKTPSAL 
      TKSSNPVCE SVICKTTSS RILSMMNHS ADPCDDFYE 
      FACGRMYEI EEESVEFHD REMLTNQLA LIIDSSPKY 
      LKDFKQFYQ TCLQHEDDF NYRPRMNKL EVLLNEVGE 
      FHFGDDSAE VDLTDLVAQ LLLRQSMPL FDVGLDVDK 
      HTSKLILQL TLPQQSFLR TGTNNWSGL SHLKNQCLK 
      KQIGLIRGL ISTFIEFLM RELAAFQNL TPEVLFKEI 
      VATRQNIEF EVLNNLDSL PSPADILRQ FIQKQYEVR 
      KLSDLKFHY PVLDWIRLF QIMTGRQIA ENTTIQIYF 
      SDYFKQIFA SLARIDKKK LNNALLAIH AYDLYINTV 
      VPKEKNTRE KFCTDLAKQ LFPEVWSQL VHSSAKDRE 
      KLNGQVGGI FGVLKTHFC KKLLTAEWI DDDTRTAIV 
      NKCNNLNLV FFTPCDEAT IVDNYEDLN VTSNFQGNL 
      INSLVHFKQ RVYSTQGTP ISAKTIFSY FVDPLDTKP 
      LTFYSNPLI AVPLGLMRK APQGLPDYL TMTRVGFSL 
      ARQIARHFD PTGMTYGVK LKSFSKSTY SEFVETMKN 
      LMDYSSPRL FEGRNISFS LNGLLSVNE RIVDNTAFR 
      LVFDSLNLQ PDQDILPWI SAQYSREKI AFIATAQEF 
      CTKTTILDF MLQVFEKRH LPAVLRVEN IMGNSEDLA 
      NKFACPDGS GMDFYVKLQ FPYLTKVEE EYEDEEQLQ 
      QNK
    CDS sequence
    • >TC013215 RA
      atgacgaaa ggttcttac agtgttggt gacaatgac 
      caccaacct tcaagaaga gatacgtct tacaaatca 
      ttcatttgt gtccttttg atttttttg ttcatttgt 
      ttgataatt ctgttagct gctattggt tggggaagt 
      ggacatggt cgtaataat ttggagaaa actcctaaa 
      gaaacatca aatcagagg gatagtcag gtcgaagtt 
      ttggaacgg aaacgttca caacaatcg aacaatact 
      tttgaaaaa ttaatcgtt gctgaaacc acgactttc 
      gtcaataaa atcgtaaat acgattcct gaaagtact 
      actgaaaaa ttggaaaaa aagatactg cgtgataaa 
      gagtggcca ataactagg cctgtggag gaaagtaca 
      acaaaaatt tttgaaagc gtaacaact attacaact 
      cccgaagca acaacagaa gaaaacgtg gttacaagt 
      actgattca gtagttgta acaaaatta gaaaaatta 
      gatacaaca caagttaca ctaaatgtt accaagggc 
      caaggtttt gttgaaact acgacttct gattatgaa 
      accactgac gaaatttcg aatcaaaac gtttcaact 
      gttacgcaa aatttagcc aaagaaacg gaattttct 
      gaaactact acgttggaa tatggaacc actgaagaa 
      atttcggga caaaatgtt tcaactgtt gcacaaaac 
      gtgtctaaa accgcaaaa ttagttgat aacacgttt 
      tccgaaact actacagaa atgttcaca caaaatgtt 
      accactgaa gtgtcgatc atggaatca gaagcaatc 
      acggaacca acccctcaa aaaacgccc tccgccttg 
      accaagtcc tcaaacccg gtttgcgaa agcgtaatt 
      tgcaaaacc acatcttcg cgaattcta agcatgatg 
      aaccattcg gccgaccct tgtgacgac ttttacgaa 
      tttgcttgt ggccgcatg tacgaaatt gaggaggaa 
      tcagtcgag ttccacgat cgtgaaatg ttaacaaac 
      caattggct ttgatcata gattcctcc cccaaatac 
      ttgaaggac ttcaaacag ttttaccaa acttgtctc 
      caacacgag gacgatttc aattaccgg ccccgaatg 
      aacaaactt gaagttttg ttgaacgaa gtcggagag 
      tttcacttt ggtgatgat tcagccgag gttgattta 
      accgatctc gtggctcag ttgcttcta cgtcagtcg 
      atgccactg tttgatgtc ggcttagac gtggacaaa 
      cacacctct aagttaatt ctccaattg actttacca 
      caacaaagc tttttgcgc accggaaca aataactgg 
      tccggtttg tcacacttg aaaaaccaa tgcttgaaa 
      aaacagatc gggctaatt cgcggactt atctcaact 
      tttatcgaa tttttgatg cgggagttg gcagcgttc 
      caaaattta actcccgaa gttctgttt aaggaaatc 
      gtagccacg cggcaaaat attgaattt gaagtttta 
      aacaatctc gattcgttg ccatcacca gccgatatt 
      ttgcgccag ttcatccaa aagcaatac gaagtgcgc 
      aagctatcc gacctgaaa ttccattac ccggttttg 
      gactggatt aggcttttc cagataatg accgggcgc 
      caaattgcc gaaaacaca accattcag atttatttc 
      agcgattat ttcaagcaa atttttgca tcgcttgcg 
      cgaatcgac aagaagaaa ttaaacaac gccctattg 
      gcaatccac gcctacgac ctgtacata aacacagtc 
      gtgccgaag gagaaaaac acgcgggaa aaattctgt 
      acggacttg gccaaacaa ttatttccc gaagtttgg 
      tcccagctt gtccacagt tcggccaaa gaccgggag 
      aagttgaac ggtcaagtc ggtggcatt ttcggtgtt 
      ttgaagacg catttttgc aaaaaactt ctcaccgct 
      gagtggatt gatgacgac acgaggacg gcaattgtg 
      aacaagtgt aacaatttg aatttggtt tttttcact 
      ccgtgtgac gaagctacg attgttgat aattacgag 
      gacttgaac gtcacttcc aactttcaa gggaactta 
      atcaatagt cttgtccac ttcaaacag agggtttat 
      tcaacacaa gggacccca atttcggcc aaaacaata 
      ttttcgtac tttgtggac cctctggac accaagccc 
      ttaacattt tattcaaat ccgttaatt gctgttcct 
      ttgggcttg atgcgcaaa gcccctcag ggtcttcct 
      gattatttg actatgacc cgcgttggt ttttcttta 
      gcccgtcaa atagcccgg cattttgac cccacggga 
      atgacctac ggggtcaaa ttaaaatct ttttcaaaa 
      tccacttat tccgaattt gtcgaaacc atgaaaaat 
      ctaatggac tattcaagc cccagactg ttcgaggga 
      cgaaacatc agtttcagt ctgaacggg ttgttgtca 
      gtgaacgaa agaatcgtc gataacacg gcttttcgc 
      ctagttttc gactcgttg aatcttcaa ccagaccag 
      gatattttg ccctggatt tcggcgcaa tattcccgc 
      gaaaaaatc gccttcata gcgacagct caagaattt 
      tgcacgaaa acaacaatt ctggacttc atgttgcaa 
      gttttcgaa aagcgccac ttgccggcg gttctccgc 
      gtcgaaaac attatggga aattcggaa gatttggcg 
      aataaattc gcgtgtcct gacggctca ggcatggat 
      ttttacgtc aagttgcag tttccttac ttgaccaaa 
      gtcgaagaa gagtacgag gatgaggaa caattgcaa 
      cagaataaa tga
    mRNA sequence
    • >TC013215 RA
      aaagcgttt ccttccgat ttgattaag aaagtcgtt 
      atttgggtc caacttcag ttttcactg ttacaaaat 
      gacgaaagg ttcttacag tgttggtga caatgacca 
      ccaaccttc aagaagaga tacgtctta caaatcatt 
      catttgtgt ccttttgat ttttttgtt catttgttt 
      gataattct gttagctgc tattggttg gggaagtgg 
      acatggtcg taataattt ggagaaaac tcctaaaga 
      aacatcaaa tcagaggga tagtcaggt cgaagtttt 
      ggaacggaa acgttcaca acaatcgaa caatacttt 
      tgaaaaatt aatcgttgc tgaaaccac gactttcgt 
      caataaaat cgtaaatac gattcctga aagtactac 
      tgaaaaatt ggaaaaaaa gatactgcg tgataaaga 
      gtggccaat aactaggcc tgtggagga aagtacaac 
      aaaaatttt tgaaagcgt aacaactat tacaactcc 
      cgaagcaac aacagaaga aaacgtggt tacaagtac 
      tgattcagt agttgtaac aaaattaga aaaattaga 
      tacaacaca agttacact aaatgttac caagggcca 
      aggttttgt tgaaactac gacttctga ttatgaaac 
      cactgacga aatttcgaa tcaaaacgt ttcaactgt 
      tacgcaaaa tttagccaa agaaacgga attttctga 
      aactactac gttggaata tggaaccac tgaagaaat 
      ttcgggaca aaatgtttc aactgttgc acaaaacgt 
      gtctaaaac cgcaaaatt agttgataa cacgttttc 
      cgaaactac tacagaaat gttcacaca aaatgttac 
      cactgaagt gtcgatcat ggaatcaga agcaatcac 
      ggaaccaac ccctcaaaa aacgccctc cgccttgac 
      caagtcctc aaacccggt ttgcgaaag cgtaatttg 
      caaaaccac atcttcgcg aattctaag catgatgaa 
      ccattcggc cgacccttg tgacgactt ttacgaatt 
      tgcttgtgg ccgcatgta cgaaattga ggaggaatc 
      agtcgagtt ccacgatcg tgaaatgtt aacaaacca 
      attggcttt gatcataga ttcctcccc caaatactt 
      gaaggactt caaacagtt ttaccaaac ttgtctcca 
      acacgagga cgatttcaa ttaccggcc ccgaatgaa 
      caaacttga agttttgtt gaacgaagt cggagagtt 
      tcactttgg tgatgattc agccgaggt tgatttaac 
      cgatctcgt ggctcagtt gcttctacg tcagtcgat 
      gccactgtt tgatgtcgg cttagacgt ggacaaaca 
      cacctctaa gttaattct ccaattgac tttaccaca 
      acaaagctt tttgcgcac cggaacaaa taactggtc 
      cggtttgtc acacttgaa aaaccaatg cttgaaaaa 
      acagatcgg gctaattcg cggacttat ctcaacttt 
      tatcgaatt tttgatgcg ggagttggc agcgttcca 
      aaatttaac tcccgaagt tctgtttaa ggaaatcgt 
      agccacgcg gcaaaatat tgaatttga agttttaaa 
      caatctcga ttcgttgcc atcaccagc cgatatttt 
      gcgccagtt catccaaaa gcaatacga agtgcgcaa 
      gctatccga cctgaaatt ccattaccc ggttttgga 
      ctggattag gcttttcca gataatgac cgggcgcca 
      aattgccga aaacacaac cattcagat ttatttcag 
      cgattattt caagcaaat ttttgcatc gcttgcgcg 
      aatcgacaa gaagaaatt aaacaacgc cctattggc 
      aatccacgc ctacgacct gtacataaa cacagtcgt 
      gccgaagga gaaaaacac gcgggaaaa attctgtac 
      ggacttggc caaacaatt atttcccga agtttggtc 
      ccagcttgt ccacagttc ggccaaaga ccgggagaa 
      gttgaacgg tcaagtcgg tggcatttt cggtgtttt 
      gaagacgca tttttgcaa aaaacttct caccgctga 
      gtggattga tgacgacac gaggacggc aattgtgaa 
      caagtgtaa caatttgaa tttggtttt tttcactcc 
      gtgtgacga agctacgat tgttgataa ttacgagga 
      cttgaacgt cacttccaa ctttcaagg gaacttaat 
      caatagtct tgtccactt caaacagag ggtttattc 
      aacacaagg gaccccaat ttcggccaa aacaatatt 
      ttcgtactt tgtggaccc tctggacac caagccctt 
      aacatttta ttcaaatcc gttaattgc tgttccttt 
      gggcttgat gcgcaaagc ccctcaggg tcttcctga 
      ttatttgac tatgacccg cgttggttt ttctttagc 
      ccgtcaaat agcccggca ttttgaccc cacgggaat 
      gacctacgg ggtcaaatt aaaatcttt ttcaaaatc 
      cacttattc cgaatttgt cgaaaccat gaaaaatct 
      aatggacta ttcaagccc cagactgtt cgagggacg 
      aaacatcag tttcagtct gaacgggtt gttgtcagt 
      gaacgaaag aatcgtcga taacacggc ttttcgcct 
      agttttcga ctcgttgaa tcttcaacc agaccagga 
      tattttgcc ctggatttc ggcgcaata ttcccgcga 
      aaaaatcgc cttcatagc gacagctca agaattttg 
      cacgaaaac aacaattct ggacttcat gttgcaagt 
      tttcgaaaa gcgccactt gccggcggt tctccgcgt 
      cgaaaacat tatgggaaa ttcggaaga tttggcgaa 
      taaattcgc gtgtcctga cggctcagg catggattt 
      ttacgtcaa gttgcagtt tccttactt gaccaaagt 
      cgaagaaga gtacgagga tgaggaaca attgcaaca 
      gaataaatg atttgcaaa aaaaaataa ttataaagg 
      tcgtttttt gataaataa agttagcgg aaaaga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY