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Gene information
General information
TC013231 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11859782..11860556
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013231
    iBeetle screen iB_10097

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013231 RA
      MPFMIGPAP IRRTLQFLE AGRLILKDQ 
      IKIMAVHYH LVGENNKGT KDFVFWCLP 
      QIQYKNPNV QIATFKNLT PSPFIRCFY 
      ETGDEMLID VDGKSKEEI YEHLIRVVG 
      KTKDVLEAE SISREKKDN PANFGVGCD 
      RQCICELPG QIPCPGVVP LPNHMRGKF 
      IHKKDN
    CDS sequence
    • >TC013231 RA
      atgccgttt atgataggt ccagctccc ataagaaga 
      acgttgcaa tttctcgaa gccgggcgt ttaatttta 
      aaagatcaa atcaaaatt atggccgta cattatcat 
      ttagttggt gaaaataac aaaggaacc aaagacttt 
      gtgttttgg tgtttgcca caaatacag tacaagaac 
      cccaacgta caaattgcg actttcaaa aacctaaca 
      ccctcacct ttcatcaga tgcttctac gagaccggt 
      gacgaaatg ctaattgat gttgatggt aaaagtaaa 
      gaggaaatt tacgagcac cttataaga gtggtgggc 
      aaaacaaag gacgtacta gaggctgaa tcaatttcc 
      agagaaaag aaggataat cctgcaaat tttggtgtt 
      ggttgcgac aggcaatgc atttgtgaa cttccgggt 
      caaatccct tgccccggc gttgtgccc ttgcctaac 
      cacatgagg ggcaaattt atccataaa aaggacaat 
      tag
    mRNA sequence
    • >TC013231 RA
      ttagtggct aaaaaaaga cttcaaaat gccgtttat 
      gataggtcc agctcccat aagaagaac gttgcaatt 
      tctcgaagc cgggcgttt aattttaaa agatcaaat 
      caaaattat ggccgtaca ttatcattt agttggtga 
      aaataacaa aggaaccaa agactttgt gttttggtg 
      tttgccaca aatacagta caagaaccc caacgtaca 
      aattgcgac tttcaaaaa cctaacacc ctcaccttt 
      catcagatg cttctacga gaccggtga cgaaatgct 
      aattgatgt tgatggtaa aagtaaaga ggaaattta 
      cgagcacct tataagagt ggtgggcaa aacaaagga 
      cgtactaga ggctgaatc aatttccag agaaaagaa 
      ggataatcc tgcaaattt tggtgttgg ttgcgacag 
      gcaatgcat ttgtgaact tccgggtca aatcccttg 
      ccccggcgt tgtgccctt gcctaacca catgagggg 
      caaatttat ccataaaaa ggacaatta gtgaatatt 
      tgtaacttc ttaggtaat aaagtgttg gaaggaatt 
      gtagggttt tacaaaaac acagctttg taatataat 
      acagaatat tta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY