Status
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Gene information
General information
TC013249 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11555362..11557879
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013249
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10093

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    egg chamber stage sequence aborted - Number of animals showing the phenotype: 1

    previtellogenic egg chamber number decreased - Number of animals showing the phenotype: 2

    vitellarium size shortened - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_10976

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013249 RA
      MLKLKPIIS HLSLKRLLS TLSPNVLEP LNNRSLIRV 
      AGPDVSNFL QGLITNDIE HLSSGPGCM YTMFLNSAG 
      RILYDAIVY RNSENNTYL VECDTKSAD ILQKHLKLY 
      RVRRKIDIT SLSDELKIY ALFDTKNFD LNSNQKLAN 
      PPLETPFKA HKELLIYRD PRITNLGLR IIAKSDVNV 
      PEQLGDNFN VTQNSSSKN YRWLRYSLG VGEGVEDLP 
      PGECFPLEC NCDYLHGVS FHKGCYVGQ ELTARVHHT 
      GVVRKRLMP LHFSKIPTK YPDEKIVQE NVSLGKLRG 
      IEGDVGLAS LRIAKTLAF KELKLGDGV AVTSRPSWW 
      PQEAPKEKI KCA
    CDS sequence
    • >TC013249 RA
      atgttaaaa ttgaaacca ataatatcc cacttatct 
      ttgaaacga ctcctatca acgttatca ccgaacgtt 
      ttagagccc ctaaataac aggtcacta attcgcgtg 
      gcaggaccg gacgtttca aattttctc caaggatta 
      attacgaac gatattgaa catttgagt tcaggaccg 
      ggctgcatg tacacaatg ttcctaaac agcgccggt 
      cggattctc tacgacgct attgtttac cgcaattcc 
      gaaaacaac acatatttg gtggaatgt gacaccaaa 
      tcggctgat attttgcaa aaacattta aaactgtac 
      agagtcagg cgtaaaatc gacattaca agcttaagc 
      gatgagtta aaaatttac gccttgttt gacacgaag 
      aactttgac ttgaattca aaccaaaaa ttggcaaat 
      ccgccgctc gaaacccca tttaaagcc cataaagaa 
      ctgctgatt tatcgcgac ccaagaatc acaaattta 
      ggtttacgg ataattgca aaaagtgac gttaatgtt 
      cccgaacaa cttggtgat aatttcaat gtcacgcaa 
      aactcgtca agcaaaaac tatcgttgg ctgcgctat 
      agtttagga gttggggag ggcgttgaa gatttgcct 
      ccaggagag tgttttccg cttgaatgt aactgtgat 
      tatttgcac ggtgttagc ttccataaa ggctgttac 
      gtggggcag gagttaaca gctcgggtg catcacact 
      ggtgtggtc aggaaacgg ttaatgcct ctccacttt 
      agcaaaata cccacgaaa tatccagac gaaaaaatc 
      gtccaagaa aatgttagt ttaggcaaa ttgaggggc 
      attgaaggg gacgtaggt ttggcttca ctgagaata 
      gcgaaaact ttggctttc aaggaacta aaattggga 
      gatggagtt gccgtaacg tccagacct agctggtgg 
      ccccaggaa gcacccaaa gaaaaaatt aagtgcgcg 
      taa
    mRNA sequence
    • >TC013249 RA
      tcacatcta acaaaggcg ccagataaa aacgtacag 
      ttttactaa aaaaatcgg aaatgaggt aaattgttg 
      tttgctcaa atttttgcg aaacaaact attctgaaa 
      tatttttag gaaacttta cagtgtgac ggtgatctt 
      catattggt caagctttt ccaatctaa tgcattctc 
      cacttttga agaattacc tgctaataa tttcacaaa 
      tgttgcaca tttggaatt tgtagaaga tttatcgca 
      agattcagc cagtaatgt ttcttgtcg agttaatgc 
      aaataatac tatttgcca cctaagtaa aaaacacgt 
      tgtgtcaat taacaacaa cttaatgca gaaatttag 
      gaacattgc gacgctatc agtgagatg gggcccaca 
      tgatgttgt ggaaattgc cgacgatgc attgaataa 
      tttaaaaaa cataaaaga attgagttt gaaaaattg 
      ttacatcaa atctcacgt ttttaaaaa tgcgagttt 
      gttgaaaat tttgtatgt ggcgcaaag tacgtttga 
      aagtataca agaagactg ttttgaagg tttaccaaa 
      tctggaaga gttgtggat ttctttaag caacgttgt 
      gataattcg tcgaggctt ttttaaaaa tctaccaaa 
      cctaacggc cttattact tcccgaaaa tggaataga 
      ggggataga gaaaaatgc aatttccta caccaaaaa 
      cctggcagt gcttaattt gagtcacaa ccggcttac 
      gtacctgcc agactgttt caaaagttt agcaaagtt 
      aaccattct ttcattacg caataactt gataaaata 
      tttaaactg gaaagactt tcgtggatt aatttcctt 
      ggagtattt ggacttatc caacaacaa aatgggttc 
      gtttgatgc caaaataat tgggactta ttttcccaa 
      cttgcggat gctgagatt ggatggaaa tgtggtaac 
      cgtgcaaaa aattaaaca cttttccga tcaactaca 
      agagatgtt caaccgttc ggttgttgc atattcagc 
      acaataaat aaacaattt gaacagcac agaaacttt 
      cgattcaaa aaaagcgct taaaatact gtcattgat 
      gacaattga tctaacctc acatattac gacattgca 
      ttttcaaaa aaattggca aaactacaa tctcgcaag 
      tgtagtaat attacacaa gtgtttatt ttgttgaga 
      ttattgtgg gttaggtcc gtcgaaaat gttaaaatt 
      gaaaccaat aatatccca cttatcttt gaaacgact 
      cctatcaac gttatcacc gaacgtttt agagcccct 
      aaataacag gtcactaat tcgcgtggc aggaccgga 
      cgtttcaaa ttttctcca aggattaat tacgaacga 
      tattgaaca tttgagttc aggaccggg ctgcatgta 
      cacaatgtt cctaaacag cgccggtcg gattctcta 
      cgacgctat tgtttaccg caattccga aaacaacac 
      atatttggt ggaatgtga caccaaatc ggctgatat 
      tttgcaaaa acatttaaa actgtacag agtcaggcg 
      taaaatcga cattacaag cttaagcga tgagttaaa 
      aatttacgc cttgtttga cacgaagaa ctttgactt 
      gaattcaaa ccaaaaatt ggcaaatcc gccgctcga 
      aaccccatt taaagccca taaagaact gctgattta 
      tcgcgaccc aagaatcac aaatttagg tttacggat 
      aattgcaaa aagtgacgt taatgttcc cgaacaact 
      tggtgataa tttcaatgt cacgcaaaa ctcgtcaag 
      caaaaacta tcgttggct gcgctatag tttaggagt 
      tggggaggg cgttgaaga tttgcctcc aggagagtg 
      ttttccgct tgaatgtaa ctgtgatta tttgcacgg 
      tgttagctt ccataaagg ctgttacgt ggggcagga 
      gttaacagc tcgggtgca tcacactgg tgtggtcag 
      gaaacggtt aatgcctct ccactttag caaaatacc 
      cacgaaata tccagacga aaaaatcgt ccaagaaaa 
      tgttagttt aggcaaatt gaggggcat tgaagggga 
      cgtaggttt ggcttcact gagaatagc gaaaacttt 
      ggctttcaa ggaactaaa attgggaga tggagttgc 
      cgtaacgtc cagacctag ctggtggcc ccaggaagc 
      acccaaaga aaaaattaa gtgcgcgta aattgttga 
      aatcaaagg cgcctatga ttggaatta agttggcag 
      cattgaatc aaattcaaa atgtcaatt cagacgctt 
      attgccaac ataaagaca ttttgaata gaaatttgt 
      ttcgtttgt gccttagat gtgtgttac ctgatttat 
      tagttaatg gaaaggtga caaagaact gtacttctg 
      tagactgta cataaaata aacc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY