Status
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Gene information
General information
TC033187
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014552,TC014553
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11546981..11550898
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033187
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
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    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10092

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033187 RA
      MSGVKISGQ TEINPSSSA EKNVPPINA KSSEASFAD 
      PLVTETISN ASTHSKASS VGSSDKDRP SVTINTSGV 
      SLNKGSADK ISHSDTTQA SQETIKTGS TAPSGTSVT 
      TPEGTQKSL QSILSKKSM SRTTLTTSK TSSLGIESS 
      DITMSSSRR SRASSKRMS SIFKRPSVP ESTISFRNP 
      LDDMTNAAL VNAVTELEK SAKHLELEN MVFLHFLEK 
      NEPSLLEGM DNVLKNVQT IQDRASLSS KRLTLTRAS 
      RQSTILSYS TSSITPTVL EKEGPRINI TQKTDLILR 
      EMDNIQAAL NKINRKCYV IRHKLKAEL EEMAIRESE 
      IQEIKDTFE RTFITNELS EKYSQKNPA DRFCRFMDE 
      TFKKTGATI DKLRLRTSS LKVQYQKIC AQLTQKKLL 
      GEHLQEVDF EQLEIDNKH LREKIDKRN LLLLELKRM 
      NGRANLLLS NQKKHLLKK VTDLKVYQD TIKMQEELV 
      RKIDDECEK VSEELEATK EEHDKIKTK SENYEVPSV 
      LEYVKKKAE LGLLKKNLK IWERRKQIK EMALTASVR 
      EMKHATGLP AAKSSWFIN PHDMHRGRG SVESTFEFM 
      FERKMRLSL IMQKKKSRR RKTQ
    • >TC033187 RB
      MDNIQAALN KINRKCYVI RHKLKAELE EMAIRESEI 
      QEIKDTFER TFITNELSE KYSQKNPAD RFCRFMDET 
      FKKTGATID KLRLRTSSL KVQYQKICA QLTQKKLLG 
      EHLQEVDFE QLEIDNKHL REKIDKRNL LLLELKRMN 
      GRANLLLSN QKKHLLKKV TDLKVYQDT IKMQEELVR 
      KIDDECEKV SEELEATKE EHDKIKTKS ENYEVPSVL 
      EYVKKKAEL GLLKKNLKI WERRKQIKE MALTASVRE 
      MKHATGLPA AKSSWFINP HDMHRGRGS VESTFEFMF 
      ERKMRLSLI MQKKKSRRR KTQ
    • >TC033187 RC
      MSGVKISGQ TEINPSSSA EKNVPPINA KSSEASFAD 
      PLVTETISN ASTHSKASS VGSSDKDRP SVTINTSGV 
      SLNKGSADK ISHSDTTQA SQETIKTGS TAPSGTSVT 
      TPEGTQKSL QSILSKKSM SRTTLTTSK TSSLGIESS 
      DITMSSSRR SRASSKRMS SIFKRPSVP ESTISFRNP 
      LDDMTNAAL VNAVTELEK SAKHLELEN MVFLHFLEK 
      NEPSLLEGM DNVLKNVQT IQDRASLSS KRLTLTRAS 
      RQSTILSYS TSSITPTVL EKEGPSYYK IFPIHNNTI 
      TDQFFKTVR QIFRNFTTI LFKQS
    CDS sequence
    • >TC033187 RA
      atgtcggga gtaaaaatt tctggacaa accgaaatt 
      aacccatct tcatctgca gaaaaaaac gttcctccc 
      atcaatgcc aagagttct gaggcttcg tttgctgat 
      cctttagtc acggaaaca atatcgaac gcctcgact 
      cattctaaa gcctcatca gttggatcc tcagacaaa 
      gacaggccc tctgttaca attaacacg tccggtgtt 
      agtcttaac aaaggttcc gctgacaaa atctctcac 
      tcagatacc acccaagct agtcaagag actatcaaa 
      acggggtca acggcaccc tccggcacg tcagtgacc 
      acgccagag gggacacaa aagtcgtta caatcaatc 
      ttgtcaaag aaaagtatg agtcggact acactaaca 
      accagcaag acttccagt ttagggata gaatcgtca 
      gacatcacc atgagctca agtagacgc agtagagct 
      tcctcgaaa cgaatgagc agcatattt aaacgcccc 
      tcagttcct gagagcacc atcagtttt cgaaacccg 
      ttggacgat atgacaaac gccgctttg gttaatgcc 
      gtaacggaa ttagaaaaa agtgccaaa catttggag 
      ctggaaaac atggttttc ctccacttt ttggaaaaa 
      aacgaaccg tcactactc gaaggtatg gataacgtg 
      ttgaaaaac gtccaaaca atccaggac cgtgcctcg 
      ctttcttcc aaaagactg acactgacg agagcttcg 
      cgccagtca acaattttg tcatactca acaagttcc 
      ataaccccc acagtatta gagaaagaa ggaccaaga 
      atcaatatt acgcagaag actgatttg atactgcga 
      gaaatggac aatattcaa gcagcgttg aataaaatc 
      aacagaaag tgctacgtt attaggcat aaattgaaa 
      gcagagttg gaggaaatg gcaattcgc gaaagtgaa 
      atacaagaa atcaaagat acgtttgaa agaacattt 
      attactaac gaattatca gaaaagtat tcgcaaaag 
      aatccagcg gatcgtttt tgtcggttt atggacgaa 
      acttttaaa aaaactggc gctacgatt gataaactg 
      cgcctaagg acgagctct ctaaaagtg cagtaccag 
      aaaatatgc gctcaatta acgcagaag aagttactg 
      ggcgagcac ttgcaagag gtggatttt gagcagctc 
      gaaattgat aataagcac ttgagggag aaaatcgat 
      aaaaggaat ttgctgctt ctcgaactg aaaagaatg 
      aacggccgc gcgaattta ctgctgagc aatcagaag 
      aagcatctg ctcaagaaa gtgaccgat ctaaaagtg 
      tatcaagac acaatcaaa atgcaagag gagttggtt 
      cggaaaatc gacgacgaa tgtgaaaag gtttccgaa 
      gaactggag gcaaccaag gaagagcac gataaaatt 
      aaaaccaag agtgaaaat tacgaggtt ccttctgtt 
      ttagagtat gtgaaaaag aaagctgag ttgggcttg 
      ctgaagaaa aatctaaag atttgggag agacggaaa 
      caaatcaaa gaaatggca cttactgcc tctgtgagg 
      gaaatgaaa cacgcgacg ggcttacca gctgccaaa 
      tcttcttgg tttattaat cctcatgat atgcatcgc 
      ggaagaggg tcagttgaa agcacattt gagtttatg 
      tttgaacgc aaaatgagg ctttcgctg ataatgcaa 
      aagaagaaa agtcgtcgt cgcaagacg cagtaa
    • >TC033187 RB
      atggacaat attcaagca gcgttgaat aaaatcaac 
      agaaagtgc tacgttatt aggcataaa ttgaaagca 
      gagttggag gaaatggca attcgcgaa agtgaaata 
      caagaaatc aaagatacg tttgaaaga acatttatt 
      actaacgaa ttatcagaa aagtattcg caaaagaat 
      ccagcggat cgtttttgt cggtttatg gacgaaact 
      tttaaaaaa actggcgct acgattgat aaactgcgc 
      ctaaggacg agctctcta aaagtgcag taccagaaa 
      atatgcgct caattaacg cagaagaag ttactgggc 
      gagcacttg caagaggtg gattttgag cagctcgaa 
      attgataat aagcacttg agggagaaa atcgataaa 
      aggaatttg ctgcttctc gaactgaaa agaatgaac 
      ggccgcgcg aatttactg ctgagcaat cagaagaag 
      catctgctc aagaaagtg accgatcta aaagtgtat 
      caagacaca atcaaaatg caagaggag ttggttcgg 
      aaaatcgac gacgaatgt gaaaaggtt tccgaagaa 
      ctggaggca accaaggaa gagcacgat aaaattaaa 
      accaagagt gaaaattac gaggttcct tctgtttta 
      gagtatgtg aaaaagaaa gctgagttg ggcttgctg 
      aagaaaaat ctaaagatt tgggagaga cggaaacaa 
      atcaaagaa atggcactt actgcctct gtgagggaa 
      atgaaacac gcgacgggc ttaccagct gccaaatct 
      tcttggttt attaatcct catgatatg catcgcgga 
      agagggtca gttgaaagc acatttgag tttatgttt 
      gaacgcaaa atgaggctt tcgctgata atgcaaaag 
      aagaaaagt cgtcgtcgc aagacgcag taa
    • >TC033187 RC
      atgtcggga gtaaaaatt tctggacaa accgaaatt 
      aacccatct tcatctgca gaaaaaaac gttcctccc 
      atcaatgcc aagagttct gaggcttcg tttgctgat 
      cctttagtc acggaaaca atatcgaac gcctcgact 
      cattctaaa gcctcatca gttggatcc tcagacaaa 
      gacaggccc tctgttaca attaacacg tccggtgtt 
      agtcttaac aaaggttcc gctgacaaa atctctcac 
      tcagatacc acccaagct agtcaagag actatcaaa 
      acggggtca acggcaccc tccggcacg tcagtgacc 
      acgccagag gggacacaa aagtcgtta caatcaatc 
      ttgtcaaag aaaagtatg agtcggact acactaaca 
      accagcaag acttccagt ttagggata gaatcgtca 
      gacatcacc atgagctca agtagacgc agtagagct 
      tcctcgaaa cgaatgagc agcatattt aaacgcccc 
      tcagttcct gagagcacc atcagtttt cgaaacccg 
      ttggacgat atgacaaac gccgctttg gttaatgcc 
      gtaacggaa ttagaaaaa agtgccaaa catttggag 
      ctggaaaac atggttttc ctccacttt ttggaaaaa 
      aacgaaccg tcactactc gaaggtatg gataacgtg 
      ttgaaaaac gtccaaaca atccaggac cgtgcctcg 
      ctttcttcc aaaagactg acactgacg agagcttcg 
      cgccagtca acaattttg tcatactca acaagttcc 
      ataaccccc acagtatta gagaaagaa ggaccaagt 
      tattataag attttcccc atccataat aacaccatc 
      actgatcag tttttcaag accgtccgc cagattttt 
      cgaaatttc accactatc ttgtttaaa caatcttaa
    mRNA sequence
    • >TC033187 RA
      ggagccgtt ttaataatt tacaatgtc gggagtaaa 
      aatttctgg acaaaccga aattaaccc atcttcatc 
      tgcagaaaa aaacgttcc tcccatcaa tgccaagag 
      ttctgaggc ttcgtttgc tgatccttt agtcacgga 
      aacaatatc gaacgcctc gactcattc taaagcctc 
      atcagttgg atcctcaga caaagacag gccctctgt 
      tacaattaa cacgtccgg tgttagtct taacaaagg 
      ttccgctga caaaatctc tcactcaga taccaccca 
      agctagtca agagactat caaaacggg gtcaacggc 
      accctccgg cacgtcagt gaccacgcc agaggggac 
      acaaaagtc gttacaatc aatcttgtc aaagaaaag 
      tatgagtcg gactacact aacaaccag caagacttc 
      cagtttagg gatagaatc gtcagacat caccatgag 
      ctcaagtag acgcagtag agcttcctc gaaacgaat 
      gagcagcat atttaaacg cccctcagt tcctgagag 
      caccatcag ttttcgaaa cccgttgga cgatatgac 
      aaacgccgc tttggttaa tgccgtaac ggaattaga 
      aaaaagtgc caaacattt ggagctgga aaacatggt 
      tttcctcca ctttttgga aaaaaacga accgtcact 
      actcgaagg tatggataa cgtgttgaa aaacgtcca 
      aacaatcca ggaccgtgc ctcgctttc ttccaaaag 
      actgacact gacgagagc ttcgcgcca gtcaacaat 
      tttgtcata ctcaacaag ttccataac ccccacagt 
      attagagaa agaaggacc aagaatcaa tattacgca 
      gaagactga tttgatact gcgagaaat ggacaatat 
      tcaagcagc gttgaataa aatcaacag aaagtgcta 
      cgttattag gcataaatt gaaagcaga gttggagga 
      aatggcaat tcgcgaaag tgaaataca agaaatcaa 
      agatacgtt tgaaagaac atttattac taacgaatt 
      atcagaaaa gtattcgca aaagaatcc agcggatcg 
      tttttgtcg gtttatgga cgaaacttt taaaaaaac 
      tggcgctac gattgataa actgcgcct aaggacgag 
      ctctctaaa agtgcagta ccagaaaat atgcgctca 
      attaacgca gaagaagtt actgggcga gcacttgca 
      agaggtgga ttttgagca gctcgaaat tgataataa 
      gcacttgag ggagaaaat cgataaaag gaatttgct 
      gcttctcga actgaaaag aatgaacgg ccgcgcgaa 
      tttactgct gagcaatca gaagaagca tctgctcaa 
      gaaagtgac cgatctaaa agtgtatca agacacaat 
      caaaatgca agaggagtt ggttcggaa aatcgacga 
      cgaatgtga aaaggtttc cgaagaact ggaggcaac 
      caaggaaga gcacgataa aattaaaac caagagtga 
      aaattacga ggttccttc tgttttaga gtatgtgaa 
      aaagaaagc tgagttggg cttgctgaa gaaaaatct 
      aaagatttg ggagagacg gaaacaaat caaagaaat 
      ggcacttac tgcctctgt gagggaaat gaaacacgc 
      gacgggctt accagctgc caaatcttc ttggtttat 
      taatcctca tgatatgca tcgcggaag agggtcagt 
      tgaaagcac atttgagtt tatgtttga acgcaaaat 
      gaggctttc gctgataat gcaaaagaa gaaaagtcg 
      tcgtcgcaa gacgcagta atgtaatta ttttataaa 
      aatacaaaa aacgttgaa ataatatgt gcaactggt 
      c
    • >TC033187 RB
      ggcacttac taaatttgg gagcacagt gattcagca 
      aattgccaa aatgaacac aaataaatt tacctccaa 
      acgtttttt ctagtttct tttagtttt gttctaaat 
      aattggcga cgctaatca atattacgc agaagactg 
      atttgatac tgcgagaaa tggacaata ttcaagcag 
      cgttgaata aaatcaaca gaaagtgct acgttatta 
      ggcataaat tgaaagcag agttggagg aaatggcaa 
      ttcgcgaaa gtgaaatac aagaaatca aagatacgt 
      ttgaaagaa catttatta ctaacgaat tatcagaaa 
      agtattcgc aaaagaatc cagcggatc gtttttgtc 
      ggtttatgg acgaaactt ttaaaaaaa ctggcgcta 
      cgattgata aactgcgcc taaggacga gctctctaa 
      aagtgcagt accagaaaa tatgcgctc aattaacgc 
      agaagaagt tactgggcg agcacttgc aagaggtgg 
      attttgagc agctcgaaa ttgataata agcacttga 
      gggagaaaa tcgataaaa ggaatttgc tgcttctcg 
      aactgaaaa gaatgaacg gccgcgcga atttactgc 
      tgagcaatc agaagaagc atctgctca agaaagtga 
      ccgatctaa aagtgtatc aagacacaa tcaaaatgc 
      aagaggagt tggttcgga aaatcgacg acgaatgtg 
      aaaaggttt ccgaagaac tggaggcaa ccaaggaag 
      agcacgata aaattaaaa ccaagagtg aaaattacg 
      aggttcctt ctgttttag agtatgtga aaaagaaag 
      ctgagttgg gcttgctga agaaaaatc taaagattt 
      gggagagac ggaaacaaa tcaaagaaa tggcactta 
      ctgcctctg tgagggaaa tgaaacacg cgacgggct 
      taccagctg ccaaatctt cttggttta ttaatcctc 
      atgatatgc atcgcggaa gagggtcag ttgaaagca 
      catttgagt ttatgtttg aacgcaaaa tgaggcttt 
      cgctgataa tgcaaaaga agaaaagtc gtcgtcgca 
      agacgcagt aatgtaatt attttataa aaatacaaa 
      aaacgttga aataatatg tgcaactgg tc
    • >TC033187 RC
      ggagccgtt ttaataatt tacaatgtc gggagtaaa 
      aatttctgg acaaaccga aattaaccc atcttcatc 
      tgcagaaaa aaacgttcc tcccatcaa tgccaagag 
      ttctgaggc ttcgtttgc tgatccttt agtcacgga 
      aacaatatc gaacgcctc gactcattc taaagcctc 
      atcagttgg atcctcaga caaagacag gccctctgt 
      tacaattaa cacgtccgg tgttagtct taacaaagg 
      ttccgctga caaaatctc tcactcaga taccaccca 
      agctagtca agagactat caaaacggg gtcaacggc 
      accctccgg cacgtcagt gaccacgcc agaggggac 
      acaaaagtc gttacaatc aatcttgtc aaagaaaag 
      tatgagtcg gactacact aacaaccag caagacttc 
      cagtttagg gatagaatc gtcagacat caccatgag 
      ctcaagtag acgcagtag agcttcctc gaaacgaat 
      gagcagcat atttaaacg cccctcagt tcctgagag 
      caccatcag ttttcgaaa cccgttgga cgatatgac 
      aaacgccgc tttggttaa tgccgtaac ggaattaga 
      aaaaagtgc caaacattt ggagctgga aaacatggt 
      tttcctcca ctttttgga aaaaaacga accgtcact 
      actcgaagg tatggataa cgtgttgaa aaacgtcca 
      aacaatcca ggaccgtgc ctcgctttc ttccaaaag 
      actgacact gacgagagc ttcgcgcca gtcaacaat 
      tttgtcata ctcaacaag ttccataac ccccacagt 
      attagagaa agaaggacc aagttatta taagatttt 
      ccccatcca taataacac catcactga tcagttttt 
      caagaccgt ccgccagat ttttcgaaa tttcaccac 
      tatcttgtt taaacaatc ttaattttt tttcaccac 
      tcttgttat tatcattcc tcaatttac actcacttg 
      caattgcat cgattgcgc gccccaaac tccaaattt 
      ttgaaacga aaacggggg tcacgtgcc ccgtttcag 
      ggtgtgtaa cattgaagt aaacgcggt ctgaagttg 
      gaatactat ttaacccct tcgctttat ttttaattt 
      tcatgagtt gtactttgt ttgccaaat ttttaccct 
      tctttttcg ccgcgatta aattttgct cgatttgtt 
      tatcaaccg cgctcttag cttcattga actgcctca 
      caggcgcag ccagtttct ccctttttt gggcgagag 
      tttcctcgt ctggctcgc tacactggg tgatatgcc 
      ggtgtttta tacagaaat gtttacgac aagtggagc 
      aaagcaaag ggaccaaac tagattcgt gacaatttt 
      cgaacgagc atttgaaca cgttttgaa ttttttgtg 
      gttctacct caacattcg ttgaggttt cacacactt 
      ggacgtgtt tgttgaaat gttccgagc aaaattgtt 
      tgccgcatt tgcattgta aagggagaa aatctaact 
      gctggcagt ttgaatggg cgcattgct gacctaaat 
      tggggagct ccatcactg gtgttcgct tagtgcact 
      gagggcaat gtcggtcca cttttgttc ttgctcctg 
      gagggttaa gtggatgtc tccggagtc ggcctcggg 
      gtcggcgag ctcgtgcgt ggggacgtt gatatcgtc 
      gtgaggcag cttctcgga acgccactt atcgtcaat 
      taaatcaac aaagtaacc atcaatagt cactgaact 
      gtccattcc tagaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY