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Gene information
General information
TC014493 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11189243..11191304
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014493
    iBeetle screen iB_10089

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014493 RA
      MAEESCLSA SLERRDYPF AEQSRQSSV TAVLNYCKS 
      KILNPYLRV LSVVGLRPL ISNQLESCF CFQFVNSVY 
      TVQLIVLLI LGYLLQYMA CFRRDRGFC YKLVNNPYL 
      VQIEDVKQI YEQICHAPL LFSFIIPSV LHLIGYVHA 
      VLVIRHSDD DQLPVLMER VFLSSNGQS SQRKLVRTL 
      WTFVTLTGI WMTLSLITI VCMMANGTI NFKWLENMP 
      EGVTVGLKI LLVVCILWH DVVQASIIS NYCLQTQLL 
      TTYLQCLQE KLLQQPVQP LEWIRHIET FKKMLRFVN 
      NELAPSVCI FTFINLACA TSGILWFFD YDNVDKETV 
      PIGGISTLN VILWLLLAL APFVQASRL SNECDILRN 
      AGQEVRTRP FVHQDTPRH DLDSILLYT ASLKLRAKL 
      FSIPITGRY LCLFFMFVG IVILVLGQC HYFTLN
    CDS sequence
    • >TC014493 RA
      atggccgaa gaatcgtgt ctatcggcg agcttggaa 
      cgtcgcgac tatcccttt gccgagcag agccgccag 
      tccagtgtt actgcagtg ctcaactac tgcaagagc 
      aagatttta aacccctat ctcagagtt ttgagcgtc 
      gtgggcttg cggccccta atttccaac caactcgag 
      tcatgtttc tgtttccag ttcgtcaac tctgtctac 
      accgttcaa ttgattgtt ttattaatc ctgggctat 
      ttgctgcaa tatatggcc tgctttcgc cgggatagg 
      ggcttctgt tacaagctc gtcaacaac ccgtacttg 
      gtccagatc gaggacgtg aaacaaatt tacgaacaa 
      atatgccat gcccctctt ttattctcg tttattatc 
      ccaagtgtt ttacatttg attggctac gttcatgcc 
      gttttggtc atcagacac tcagacgac gaccaattg 
      cctgtttta atggagcga gttttcctc tccagtaac 
      ggacaaagc agccaaagg aaactcgtg cggacgcta 
      tggaccttc gtcaccttg actggaatc tggatgacc 
      ttatccctt atcacaatc gtgtgcatg atggccaac 
      ggcactatc aatttcaaa tggctggaa aacatgcca 
      gagggcgtc acagtgggg ctcaaaatc ctgctagtt 
      gtgtgtatt ttatggcat gatgtggtc caggcctcc 
      attatttca aactactgt ctgcaaacg caactctta 
      accacgtac ttgcaatgt ttgcaagag aaactcctc 
      caacaacca gtccaaccc ttggagtgg atcagacac 
      attgaaacg tttaagaaa atgttacgt ttcgtcaat 
      aatgaactg gcgccttcg gtgtgcatt ttcacgttt 
      attaattta gcgtgtgcc acttcgggc attttgtgg 
      ttttttgat tacgataat gtcgataag gagacggtc 
      cccattggg gggattagc accctcaat gtgattttg 
      tggcttttg ctggcgttg gcccccttt gtacaggct 
      tcccgcttg tcgaacgag tgcgacatt ttacgcaac 
      gccggccaa gaagtgcgg acgcggcct ttcgtccac 
      caggacact ccacgtcac gacctcgac tcgatcctc 
      ttgtacaca gccagtcta aagttgagg gctaaacta 
      ttctcaatc ccaataact ggccgatat ctgtgtttg 
      tttttcatg tttgtggga atagtgatt ttggtcttg 
      ggccaatgt cattatttc acattaaat tga
    mRNA sequence
    • >TC014493 RA
      ttttccaac cgattattt aacgtaaac actagtcgt 
      ctagtggtg attttttcg tgcaatatg ttttgctcg 
      tccggaggt gcgggaaaa tgagcagaa aatggccga 
      agaatcgtg tctatcggc gagcttgga acgtcgcga 
      ctatccctt tgccgagca gagccgcca gtccagtgt 
      tactgcagt gctcaacta ctgcaagag caagatttt 
      aaaccccta tctcagagt tttgagcgt cgtgggctt 
      gcggcccct aatttccaa ccaactcga gtcatgttt 
      ctgtttcca gttcgtcaa ctctgtcta caccgttca 
      attgattgt tttattaat cctgggcta tttgctgca 
      atatatggc ctgctttcg ccgggatag gggcttctg 
      ttacaagct cgtcaacaa cccgtactt ggtccagat 
      cgaggacgt gaaacaaat ttacgaaca aatatgcca 
      tgcccctct tttattctc gtttattat cccaagtgt 
      tttacattt gattggcta cgttcatgc cgttttggt 
      catcagaca ctcagacga cgaccaatt gcctgtttt 
      aatggagcg agttttcct ctccagtaa cggacaaag 
      cagccaaag gaaactcgt gcggacgct atggacctt 
      cgtcacctt gactggaat ctggatgac cttatccct 
      tatcacaat cgtgtgcat gatggccaa cggcactat 
      caatttcaa atggctgga aaacatgcc agagggcgt 
      cacagtggg gctcaaaat cctgctagt tgtgtgtat 
      tttatggca tgatgtggt ccaggcctc cattatttc 
      aaactactg tctgcaaac gcaactctt aaccacgta 
      cttgcaatg tttgcaaga gaaactcct ccaacaacc 
      agtccaacc cttggagtg gatcagaca cattgaaac 
      gtttaagaa aatgttacg tttcgtcaa taatgaact 
      ggcgccttc ggtgtgcat tttcacgtt tattaattt 
      agcgtgtgc cacttcggg cattttgtg gttttttga 
      ttacgataa tgtcgataa ggagacggt ccccattgg 
      ggggattag caccctcaa tgtgatttt gtggctttt 
      gctggcgtt ggccccctt tgtacaggc ttcccgctt 
      gtcgaacga gtgcgacat tttacgcaa cgccggcca 
      agaagtgcg gacgcggcc tttcgtcca ccaggacac 
      tccacgtca cgacctcga ctcgatcct cttgtacac 
      agccagtct aaagttgag ggctaaact attctcaat 
      cccaataac tggccgata tctgtgttt gtttttcat 
      gtttgtggg aatagtgat tttggtctt gggccaatg 
      tcattattt cacattaaa ttgaactgt tttaatcta 
      tttaataaa tcattttta tcagcaaaa cagtctagt 
      aatatctgc ttggagggt aacaaatca caaattaac 
      acatttact agaattata ttcacagac tcgccctaa 
      tcataacta caaactatg catgcaaat aattgcaca 
      ataaacatt gagacatga t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY