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Gene information
General information
TC014492 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 11186593..11189188
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014492
    iBeetle screen iB_10088

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    4

    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014492 RA
      MQVSDPNNV KIYNLSAGK SLPEWLSER KRRALLKKD 
      VDIRRRIEL IQDFDMPGL STTVKVSKD GQYVLATGI 
      YKPRIKCFD VNNLSLKFE RCFDSEAVT FEILSDDYS 
      KLVFLQCDR YIEFHAAHG RYYRLRIPK FGRDMRYHY 
      PSCDLFVVG ASSDIYRLN LERGQFMTP YTSTASAIN 
      KCAINPVHN LLVCGTQEG RVEAWDPRS KGLVGTLDC 
      AFNCVTENK ELEGFPSVS ALAFDGALT MGVGTATGQ 
      VLLYDIRSN KPFCIKDHM YGLPIKDVE FHHQQDLIF 
      SMDSSVLKI WDKNNGKLF TSIEASTEF NNLCVVPKT 
      GLFFIANEN TKIQTYYIP SLGPAPRWA SFLDSLTEE 
      LEESNSENI YDDYKFVTK QELENLGLD HLIGTNLLR 
      AYMHGYFMD VRLYKKAKS VANPFEFEE YRKKKIRET 
      IEKERVNRV QVNKLPKVN KDLALKLMN DQVKDKKKK 
      ENANLLEDT RFKALFENP DFEVDKNAD EFRLLNPVL 
      SRLDKSKKK EMLKKIQSE DFEPIDEEL EGKNSSEES 
      SDDYEEESS EDETLTKEI KQQHRIINR EHRLKQRQE 
      DYEEMKAQP QMFELRQGE EYKGIHSLR KKINKAPLE 
      ERVKSETSS VRLLGSVGS REMTFSTHK KRKVSDLKN 
      KKHMQERKR LSRPVPGRR NPFSRKKL
    CDS sequence
    • >TC014492 RA
      atgcaagtt tccgacccg aataacgtg aaaatttat 
      aatttgagc gccggcaag tcactgcca gagtggctt 
      tcggaacgc aaacgccgg gctttgctc aagaaagat 
      gtggacatt cggcgaaga atcgagctt atccaggac 
      ttcgacatg ccgggcctt agcacgact gtgaaggtc 
      tcaaaagac ggacaatac gtcctggca actggcatt 
      tacaagccc cggattaaa tgcttcgac gttaacaac 
      ctctcgctc aaattcgag cgctgcttc gattcggaa 
      gccgtaacg tttgagatt ttgagcgac gattacagc 
      aaattggtt tttttacaa tgtgacagg tacattgaa 
      tttcatgcc gcacatggg cgctattac cgcctcagg 
      atccccaaa tttggccgc gatatgcgc tatcactac 
      cccagctgc gacctattc gtagtgggg gccagttca 
      gacatttac agacttaat ttagaaagg ggccagttc 
      atgacgcct tacacaagc actgcctct gccattaat 
      aagtgtgca attaacccc gtccacaat ttactagtc 
      tgtggcaca caagagggc cgcgtcgag gcgtgggac 
      cccaggagc aagggcttg gtggggacg cttgactgc 
      gcctttaac tgcgtcacc gaaaataaa gaactggaa 
      ggtttcccg tctgttagt gcccttgcg tttgacggg 
      gccctcaca atgggggtg gggacagcc actggacaa 
      gtattactt tacgatatt cgatcaaac aaacccttc 
      tgtattaaa gaccacatg tatggactg cctatcaag 
      gacgtagag ttccaccac cagcaagac ctaattttt 
      tcaatggac agttcagtt ttgaaaatc tgggacaaa 
      aataatggg aaattattt acgtcaatc gaggcctca 
      accgagttt aataatttg tgtgtggtc ccgaaaacc 
      ggtctcttt ttcattgcc aacgaaaac acaaaaatt 
      cagacgtat tacatccct agtttaggc ccagcccca 
      cgttgggcc agttttctt gactcactc acggaggaa 
      ttggaggaa tcaaactct gaaaacatc tacgatgat 
      tacaaattt gttacgaaa caagagctg gagaatctc 
      gggttggat catttaatt gggacgaat ttgctaagg 
      gcttacatg cacggctat ttcatggat gtcaggctg 
      tacaaaaag gccaaatcg gtcgcaaat ccgttcgag 
      tttgaggaa taccgcaaa aagaaaatt cgggaaacg 
      atcgagaaa gaaagggtg aatcgggtc caggtcaat 
      aaattacca aaagtcaac aaagacctc gcacttaaa 
      ctaatgaac gaccaagtg aaagacaag aagaagaaa 
      gaaaacgcc aatcttctt gaagacacg aggtttaag 
      gccctcttc gaaaacccc gactttgaa gttgacaaa 
      aacgccgat gaattccgg ttactaaac ccggtcttg 
      tcacggctg gacaaatca aagaagaaa gaaatgcta 
      aagaagatc cagagcgaa gattttgaa cccatcgat 
      gaagaacta gaaggcaag aacagttcg gaagaatcg 
      tccgacgat tacgaagag gagagttcg gaagacgaa 
      accttgact aaagaaatc aaacagcaa catagaatt 
      atcaaccgg gagcacaga ctgaaacaa cgccaggaa 
      gactacgaa gaaatgaag gcacaaccc caaatgttc 
      gaactgaga caaggcgaa gaatacaag gggatacac 
      agcctcagg aagaaaatt aacaaggcc cccctggag 
      gaacgagtc aagagcgaa acctcctcg gtccgcctc 
      ttaggctca gtcggaagc cgcgaaatg acgttctcc 
      acccacaaa aagcgcaaa gtctccgac ttgaagaac 
      aagaaacac atgcaggag cgcaaacgc ctgagtcgg 
      cccgtcccc ggccgcagg aacccattt tcccgcaaa 
      aaactgtaa
    mRNA sequence
    • >TC014492 RA
      agggactaa ccaaaatgc aagtttccg acccgaata 
      acgtgaaaa tttataatt tgagcgccg gcaagtcac 
      tgccagagt ggctttcgg aacgcaaac gccgggctt 
      tgctcaaga aagatgtgg acattcggc gaagaatcg 
      agcttatcc aggacttcg acatgccgg gccttagca 
      cgactgtga aggtctcaa aagacggac aatacgtcc 
      tggcaactg gcatttaca agccccgga ttaaatgct 
      tcgacgtta acaacctct cgctcaaat tcgagcgct 
      gcttcgatt cggaagccg taacgtttg agattttga 
      gcgacgatt acagcaaat tggtttttt tacaatgtg 
      acaggtaca ttgaatttc atgccgcac atgggcgct 
      attaccgcc tcaggatcc ccaaatttg gccgcgata 
      tgcgctatc actacccca gctgcgacc tattcgtag 
      tgggggcca gttcagaca tttacagac ttaatttag 
      aaaggggcc agttcatga cgccttaca caagcactg 
      cctctgcca ttaataagt gtgcaatta accccgtcc 
      acaatttac tagtctgtg gcacacaag agggccgcg 
      tcgaggcgt gggacccca ggagcaagg gcttggtgg 
      ggacgcttg actgcgcct ttaactgcg tcaccgaaa 
      ataaagaac tggaaggtt tcccgtctg ttagtgccc 
      ttgcgtttg acggggccc tcacaatgg gggtgggga 
      cagccactg gacaagtat tactttacg atattcgat 
      caaacaaac ccttctgta ttaaagacc acatgtatg 
      gactgccta tcaaggacg tagagttcc accaccagc 
      aagacctaa ttttttcaa tggacagtt cagttttga 
      aaatctggg acaaaaata atgggaaat tatttacgt 
      caatcgagg cctcaaccg agtttaata atttgtgtg 
      tggtcccga aaaccggtc tctttttca ttgccaacg 
      aaaacacaa aaattcaga cgtattaca tccctagtt 
      taggcccag ccccacgtt gggccagtt ttcttgact 
      cactcacgg aggaattgg aggaatcaa actctgaaa 
      acatctacg atgattaca aatttgtta cgaaacaag 
      agctggaga atctcgggt tggatcatt taattggga 
      cgaatttgc taagggctt acatgcacg gctatttca 
      tggatgtca ggctgtaca aaaaggcca aatcggtcg 
      caaatccgt tcgagtttg aggaatacc gcaaaaaga 
      aaattcggg aaacgatcg agaaagaaa gggtgaatc 
      gggtccagg tcaataaat taccaaaag tcaacaaag 
      acctcgcac ttaaactaa tgaacgacc aagtgaaag 
      acaagaaga agaaagaaa acgccaatc ttcttgaag 
      acacgaggt ttaaggccc tcttcgaaa accccgact 
      ttgaagttg acaaaaacg ccgatgaat tccggttac 
      taaacccgg tcttgtcac ggctggaca aatcaaaga 
      agaaagaaa tgctaaaga agatccaga gcgaagatt 
      ttgaaccca tcgatgaag aactagaag gcaagaaca 
      gttcggaag aatcgtccg acgattacg aagaggaga 
      gttcggaag acgaaacct tgactaaag aaatcaaac 
      agcaacata gaattatca accgggagc acagactga 
      aacaacgcc aggaagact acgaagaaa tgaaggcac 
      aaccccaaa tgttcgaac tgagacaag gcgaagaat 
      acaagggga tacacagcc tcaggaaga aaattaaca 
      aggcccccc tggaggaac gagtcaaga gcgaaacct 
      cctcggtcc gcctcttag gctcagtcg gaagccgcg 
      aaatgacgt tctccaccc acaaaaagc gcaaagtct 
      ccgacttga agaacaaga aacacatgc aggagcgca 
      aacgcctga gtcggcccg tccccggcc gcaggaacc 
      cattttccc gcaaaaaac tgtaaacca acttctcaa 
      ataaaaata ttttctgtt a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY