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Gene information
General information
TC014480 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10917196..10918743
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014480
    iBeetle screen iB_10085

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014480 RA
      MSKSQRDHL MHSTGTTPY EIIGVILPS LFYTTITTI 
      IVTLLQTRP KNLPLQFTI EFLTLVLPI ILNVTVLSD 
      YTFELLSTF LIITLSLIL WLRGSKPLS AKPPQAPLT 
      HDYITNSRS TINIISVIA ILAVDFLIF PRRFAKTKT 
      YGYSLMDVG VGLFIFSNG IVDAGRTDL GRSLKGAVP 
      LLVLGVGRF FGTKLLGYH VPVTEYGVH WNFFISLAV 
      IKIVVSLVF SVIEVKYIF INATLLLLS HESLLQGGL 
      KNFVMDDHK RSNFLTANR EGIISCFGY VSLYMFSAN 
      FSYFVGLKK GGEGVKKTV IKFVFLATL MLFLSQIWQ 
      KYCGISRKL ANSAYCFWV LFIGIFMTG LYYIFAKIL 
      EDTFRNKLH VNLQLPLIF QAINYNGLV FFLVSNLLT 
      GLVNVLCDT LQVSSVVSL GIITVYMLV NCAIVCVLF 
      TKQIKLKL
    CDS sequence
    • >TC014480 RA
      atgtccaag tcccaaagg gaccacctg atgcattcc 
      acggggacc accccctac gaaataatc ggcgtaatc 
      ctcccgagc cttttctac accaccatc accacaata 
      atcgtgacc ctgctccaa acccgcccc aaaaacctc 
      cccctccaa ttcaccata gaattcctc actctagtc 
      ctccccatt attttaaac gtgacagta ttatcggac 
      tacactttc gaactcctc tccacattt ctcatcatc 
      accctatcg ctgattttg tggctgcgg ggctccaag 
      cccctctcc gccaaacca ccacaagcc cccctaacc 
      cacgactac atcactaac agccgctcc accatcaac 
      ataatttca gtgattgcg attctggcc gttgatttt 
      ttaattttc ccgcgccgg tttgccaaa accaagacg 
      tacgggtac agtctcatg gatgtgggc gtggggctg 
      ttcattttc tccaacggg atcgtggat gcgggccgg 
      accgacctg gggaggtcg ctcaagggg gctgtaccg 
      ctattggta ctgggcgtg gggcgcttt tttggaaca 
      aaactgctg ggttatcac gtcccggtg actgaatat 
      ggggtccac tggaatttt ttcatatcg ctggcggtg 
      atcaaaatc gtagtttcg ctcgttttt agcgtgatt 
      gaagtcaaa tatattttt atcaatgcg actttgttg 
      ttgctatcg cacgagtct ttgctacag ggtggcttg 
      aaaaatttc gttatggat gatcacaaa cgtagcaat 
      tttttgacc gctaataga gaggggatc atctcgtgt 
      tttgggtac gtctccttg tacatgttt tcggccaat 
      tttagttac ttcgtcggt ttgaaaaaa gggggggaa 
      ggggttaag aaaaccgtg atcaagttt gtttttttg 
      gccactttg atgctgttt ttgtcacaa atctggcaa 
      aagtattgt gggatttcc cggaaattg gcaaactcg 
      gcctactgt ttttgggtt ctttttatt gggattttc 
      atgactgga ctttactat atttttgcg aaaatttta 
      gaagatact ttcaggaat aagctacat gtcaacctc 
      caactaccg ctcattttt caagctatt aactacaac 
      ggtttagtt tttttcctg gtcagtaat ttattaacc 
      ggcctagtc aacgtttta tgtgataca cttcaagtt 
      tcgtctgtt gttagttta ggaattatt accgtttat 
      atgttggtc aactgcgca attgtatgt gtccttttt 
      acaaaacaa atcaaatta aaactctaa
    mRNA sequence
    • >TC014480 RA
      acacaaaaa tcactgagc gaattgtgg ttatgtccc 
      gaaaaatca ataaattgt ttataattt attttgtgc 
      aattgctac agggctatg actagtaca gatttttga 
      ccggtcaag atgtccaag tcccaaagg gaccacctg 
      atgcattcc acggggacc accccctac gaaataatc 
      ggcgtaatc ctcccgagc cttttctac accaccatc 
      accacaata atcgtgacc ctgctccaa acccgcccc 
      aaaaacctc cccctccaa ttcaccata gaattcctc 
      actctagtc ctccccatt attttaaac gtgacagta 
      ttatcggac tacactttc gaactcctc tccacattt 
      ctcatcatc accctatcg ctgattttg tggctgcgg 
      ggctccaag cccctctcc gccaaacca ccacaagcc 
      cccctaacc cacgactac atcactaac agccgctcc 
      accatcaac ataatttca gtgattgcg attctggcc 
      gttgatttt ttaattttc ccgcgccgg tttgccaaa 
      accaagacg tacgggtac agtctcatg gatgtgggc 
      gtggggctg ttcattttc tccaacggg atcgtggat 
      gcgggccgg accgacctg gggaggtcg ctcaagggg 
      gctgtaccg ctattggta ctgggcgtg gggcgcttt 
      tttggaaca aaactgctg ggttatcac gtcccggtg 
      actgaatat ggggtccac tggaatttt ttcatatcg 
      ctggcggtg atcaaaatc gtagtttcg ctcgttttt 
      agcgtgatt gaagtcaaa tatattttt atcaatgcg 
      actttgttg ttgctatcg cacgagtct ttgctacag 
      ggtggcttg aaaaatttc gttatggat gatcacaaa 
      cgtagcaat tttttgacc gctaataga gaggggatc 
      atctcgtgt tttgggtac gtctccttg tacatgttt 
      tcggccaat tttagttac ttcgtcggt ttgaaaaaa 
      gggggggaa ggggttaag aaaaccgtg atcaagttt 
      gtttttttg gccactttg atgctgttt ttgtcacaa 
      atctggcaa aagtattgt gggatttcc cggaaattg 
      gcaaactcg gcctactgt ttttgggtt ctttttatt 
      gggattttc atgactgga ctttactat atttttgcg 
      aaaatttta gaagatact ttcaggaat aagctacat 
      gtcaacctc caactaccg ctcattttt caagctatt 
      aactacaac ggtttagtt tttttcctg gtcagtaat 
      ttattaacc ggcctagtc aacgtttta tgtgataca 
      cttcaagtt tcgtctgtt gttagttta ggaattatt 
      accgtttat atgttggtc aactgcgca attgtatgt 
      gtccttttt acaaaacaa atcaaatta aaactctaa 
      tcgactttc aagtatttt atttatctc acattttta 
      cgtacaaat aaataattt agtaaaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY