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Gene information
General information
TC014452 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10590447..10592335
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014452
    iBeetle screen iB_10081

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    leg musculature irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature sometimes orientation irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014452 RA
      MFVFSAMKI LLAKLPRPW IVDEKKDDG YTALHLAAL 
      NNHVEVAEQ LVLHGKANM DLQNVNLQT ALHLAVERQ 
      HTQIVRLLV REGANLNIA DKDGDTPLH EALRHHTLS 
      QLRQLHDVQ DVGKLLMGL GTQGCDKKS SASIACFLA 
      GNGADLDLK NKKGQTPLD LCPDPNLCK TLKKCHKDK 
      QSGEMDMGP NQNNQSTNI TSTLDECLV CSDAKRDML 
      FQPCGHVTC CSLCGPRVK KCLICRESV STRIKIEEC 
      LVCSDKKST VLFKPCGHM CACESCSQI MKKCVLCRA 
      QIELMVPLT VCSGGLGTI SEVKADAEE EPKPSTSDA 
      GQNQGPLMN NGGRDTSNN SDIQKLQQQ LQDIKEQTM 
      CPVCLDRLK NMIFLCGHG LCQMCGDQM TECPICRKA 
      VEKRILLY
    CDS sequence
    • >TC014452 RA
      atgtttgtt ttcagcgct atgaagatc ctgctggcc 
      aaactgccg cggccttgg atcgtggac gagaagaag 
      gacgacggt tacaccgcc ctgcacctg gcagccctc 
      aacaaccac gtggaggtg gctgagcag ctcgtcctc 
      cacgggaaa gccaacatg gacctgcag aacgtcaac 
      ctccaaacc gccctccac ctggccgtc gaacgccaa 
      cacacgcaa attgtccgc cttctcgtc cgagaaggt 
      gcaaattta aacatcgcc gacaaagac ggtgacacg 
      ccgctccac gaagccctc cgtcaccac actctttcg 
      caattacgg caattacac gacgtccaa gacgtcggc 
      aagctcctc atgggtcta ggaacccaa ggatgcgac 
      aagaagtcg tcggcgagc atcgcctgt ttcttggcc 
      ggaaacggc gccgattta gatttaaaa aacaagaaa 
      ggacaaacg cctttagat ctctgcccc gatcccaat 
      ctatgcaaa acgttgaag aagtgtcac aaagacaag 
      caaagcggg gagatggac atgggcccc aatcagaac 
      aatcaaagc acgaatatc acttcgacg ctcgacgag 
      tgtttggtc tgttcggac gccaaaagg gacatgctt 
      tttcaacct tgcggtcac gtgacctgc tgttccctg 
      tgcggaccg agggtcaaa aagtgcctc atttgtcgc 
      gaatcagtc agcactaga atcaagatc gaggaatgt 
      ctcgtttgc tccgataaa aaatcgacc gttttattc 
      aaaccctgc ggccacatg tgtgcctgc gagagttgc 
      tctcaaatt atgaagaaa tgtgtttta tgtcgcgcg 
      caaatcgaa ctgatggtg ccgttgacc gtttgcagc 
      gggggcttg ggcaccatc tcggaggtg aaagccgac 
      gctgaagag gaacccaaa ccctcaacc agcgacgca 
      gggcagaat caagggccg ctcatgaat aacggaggg 
      cgggacacc agcaataat agcgatata caaaaattg 
      cagcaacaa ttgcaagac attaaggaa caaacaatg 
      tgtcccgtc tgtttggat cgactgaaa aacatgatt 
      ttcctgtgc ggacatgga ttgtgtcaa atgtgcggc 
      gaccagatg accgaatgc cctatttgc aggaaagcc 
      gtcgaaaaa cgaattcta ttgtactaa
    mRNA sequence
    • >TC014452 RA
      tccctaatt tgtcacgaa tgtaaaaca agggagtaa 
      aaaatgttt gttttcagc gctatgaag atcctgctg 
      gccaaactg ccgcggcct tggatcgtg gacgagaag 
      aaggacgac ggttacacc gccctgcac ctggcagcc 
      ctcaacaac cacgtggag gtggctgag cagctcgtc 
      ctccacggg aaagccaac atggacctg cagaacgtc 
      aacctccaa accgccctc cacctggcc gtcgaacgc 
      caacacacg caaattgtc cgccttctc gtccgagaa 
      ggtgcaaat ttaaacatc gccgacaaa gacggtgac 
      acgccgctc cacgaagcc ctccgtcac cacactctt 
      tcgcaatta cggcaatta cacgacgtc caagacgtc 
      ggcaagctc ctcatgggt ctaggaacc caaggatgc 
      gacaagaag tcgtcggcg agcatcgcc tgtttcttg 
      gccggaaac ggcgccgat ttagattta aaaaacaag 
      aaaggacaa acgccttta gatctctgc cccgatccc 
      aatctatgc aaaacgttg aagaagtgt cacaaagac 
      aagcaaagc ggggagatg gacatgggc cccaatcag 
      aacaatcaa agcacgaat atcacttcg acgctcgac 
      gagtgtttg gtctgttcg gacgccaaa agggacatg 
      ctttttcaa ccttgcggt cacgtgacc tgctgttcc 
      ctgtgcgga ccgagggtc aaaaagtgc ctcatttgt 
      cgcgaatca gtcagcact agaatcaag atcgaggaa 
      tgtctcgtt tgctccgat aaaaaatcg accgtttta 
      ttcaaaccc tgcggccac atgtgtgcc tgcgagagt 
      tgctctcaa attatgaag aaatgtgtt ttatgtcgc 
      gcgcaaatc gaactgatg gtgccgttg accgtttgc 
      agcgggggc ttgggcacc atctcggag gtgaaagcc 
      gacgctgaa gaggaaccc aaaccctca accagcgac 
      gcagggcag aatcaaggg ccgctcatg aataacgga 
      gggcgggac accagcaat aatagcgat atacaaaaa 
      ttgcagcaa caattgcaa gacattaag gaacaaaca 
      atgtgtccc gtctgtttg gatcgactg aaaaacatg 
      attttcctg tgcggacat ggattgtgt caaatgtgc 
      ggcgaccag atgaccgaa tgccctatt tgcaggaaa 
      gccgtcgaa aaacgaatt ctattgtac taatttatc 
      tcccttgtt tgaactgaa atcgatcaa attaccacc 
      aaatccggg tttcatgac gtaagaacg acaatatca 
      aatatttta ctcgatttt taattcgac tgaaattct 
      tttgtatat tttgttaat ctcagttct aggtgctta 
      cgcaactgt agtatgaat actatatca ttttttatt 
      taacttagg tcgtaataa acgttgtag caatgctgc 
      aattacagt accatcaat tatatgatg ctctagatc 
      gcataagaa tgaaaaaat actccgaga gtaaaaagt 
      ttttcactt tttaaccca cacgcgacg aatattcat 
      tttcagata tttattata gccaaatgt tagtactat 
      cttgttatc atttcagtt gtattttta tacattaga 
      taagagtta atcagttaa ttagttaat cgaattatt 
      atcactact attattgca accccagta cgagtaata 
      aacatattg ttaaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY