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Gene information
General information
TC014444 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10475627..10478927
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014444
    iBeetle screen iB_10080

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014444 RA
      MAEDLDVEA MLEAPFRKD EEVNGKIKE NGDSRKHRS 
      SDKKRRSSS RSRRSRSRS RDKRRSRDK DRKRSKSRD 
      RVRSKDRPG RDRRSRSRD RRSRSKDRR SRSKDKRSR 
      SRDRRDRRG RRSRSRDRR RSPSPVAAK PFGRRNRSP 
      LGLRSNSPV EELSPEERD ARTVFVMQL SQRIRARDL 
      EEFFSSVGK VRDVRLIVC NKTRRFKGI AYIEFKDPE 
      SVTLALGLS GQKLLGVPI IVQHTQAEK NRMGNSMPN 
      LMPKNMTGP MRLYVGSLH FNITEDMLR SIFEPFGKI 
      DNIQLIMDP ETGRSKGYG FIAFRNCED AKKALEQLN 
      GFELAGRPM KVGNVTERL DLQQQGPSI LDSDELDRS 
      GIDLGATGR LQLMFKLAE GAGMQVPQA AANALSIAT 
      GQPVVPQVQ TNSTPPIAT QCFMLSNMF DPATESTQT 
      WDVEIRDDV IEECNKHGG VLHVYVDKG SPQGNVYVK 
      CPSIATAVA SVNSLHGRW FAGRVITAA YVPLLNYHS 
      LFPDAMNAA QLLLPSSRK
    CDS sequence
    • >TC014444 RA
      atggccgaa gacctggac gtcgaggcc atgctggaa 
      gcccccttc cgaaaagat gaagaagtc aatggcaaa 
      atcaaggaa aatggagac tcacggaaa cacagaagc 
      tcagacaag aaacgtcgc agctcgagc aggagtcgc 
      cgcagccgc agccggtca cgcgacaag cgccgaagc 
      cgcgacaag gatcgcaag aggagcaag agtcgtgat 
      cgcgtccgt tcgaaagat cgtcccggt cgagaccgc 
      cgatcgcgg tccagagac cggcgttca cgttccaaa 
      gaccgaaga tcgcgctct aaagacaag agaagtcga 
      tcgcgagac agaagagat cgccgaggt cgaagaagc 
      cgcagtcgg gaccgcaga cgatcgcca tcaccggtc 
      gccgctaag ccttttggt cggcgaaac cgatcgccg 
      ttgggtctt cggtcgaat tcgccggtt gaagagctc 
      agtcctgag gaaagggac gccagaacg gtttttgtt 
      atgcaatta tcgcaaagg atacgcgcg agggatttg 
      gaagagttc ttcagttcg gttggaaaa gttcgggat 
      gtgcggttg atcgtttgt aataaaact agaaggttc 
      aaagggatt gcttatatc gagttcaag gatccggaa 
      agtgtcact ttggcgtta ggattgtcg ggtcaaaaa 
      ttactaggt gttccaatt atagtccag catacacaa 
      gctgagaaa aatcgaatg ggcaattca atgcccaac 
      ttgatgccc aaaaatatg acagggccg atgcggtta 
      tacgtgggc tcgctacat ttcaacata actgaagac 
      atgctcagg agcatattt gaacccttt ggcaaaata 
      gacaatatt cagttaatt atggatcca gaaacgggg 
      cgatcaaag ggttacggg ttcattgcg tttcgtaat 
      tgcgaggac gcgaaaaaa gctttggaa caattgaac 
      gggtttgaa ttagcgggt agaccaatg aaggtcgga 
      aatgtaaca gaaagacta gatttacaa cagcaaggt 
      ccttcaata cttgattcc gacgagttg gatcgctcg 
      ggtatagat ttaggtgca acgggacgt ttgcaattg 
      atgttcaaa ttggccgag ggggcggga atgcaagta 
      ccacaagct gcagctaac gctctgagt atagcgaca 
      ggacagcca gtggttcca caagtccaa acgaattca 
      acaccacct atcgcaacg caatgtttc atgttgagt 
      aacatgttt gacccggca acggagtca acgcagact 
      tgggacgtt gagattaga gatgacgta atagaagag 
      tgtaacaaa cacgggggt gtgctacac gtctatgta 
      gataagggg tcgccacag ggaaatgtt tacgttaag 
      tgtccgtct atagccacg gcagtggcg tccgttaat 
      tcgctgcac ggccgatgg tttgcagga cgggttatc 
      acagcggcg tacgtccct ctattgaat tatcattcg 
      ttgtttcct gatgctatg aatgcagcg caattgcta 
      ttgccatcc agtaggaag taa
    mRNA sequence
    • >TC014444 RA
      gccattttg tcgtttcga atcgactcg cttgtatcg 
      gttgaaatt tgagccgtt ttaacgcat ttttactga 
      ttttggagt aatagtgcc gaacgtccg caatggccg 
      aagacctgg acgtcgagg ccatgctgg aagccccct 
      tccgaaaag atgaagaag tcaatggca aaatcaagg 
      aaaatggag actcacgga aacacagaa gctcagaca 
      agaaacgtc gcagctcga gcaggagtc gccgcagcc 
      gcagccggt cacgcgaca agcgccgaa gccgcgaca 
      aggatcgca agaggagca agagtcgtg atcgcgtcc 
      gttcgaaag atcgtcccg gtcgagacc gccgatcgc 
      ggtccagag accggcgtt cacgttcca aagaccgaa 
      gatcgcgct ctaaagaca agagaagtc gatcgcgag 
      acagaagag atcgccgag gtcgaagaa gccgcagtc 
      gggaccgca gacgatcgc catcaccgg tcgccgcta 
      agccttttg gtcggcgaa accgatcgc cgttgggtc 
      ttcggtcga attcgccgg ttgaagagc tcagtcctg 
      aggaaaggg acgccagaa cggtttttg ttatgcaat 
      tatcgcaaa ggatacgcg cgagggatt tggaagagt 
      tcttcagtt cggttggaa aagttcggg atgtgcggt 
      tgatcgttt gtaataaaa ctagaaggt tcaaaggga 
      ttgcttata tcgagttca aggatccgg aaagtgtca 
      ctttggcgt taggattgt cgggtcaaa aattactag 
      gtgttccaa ttatagtcc agcatacac aagctgaga 
      aaaatcgaa tgggcaatt caatgccca acttgatgc 
      ccaaaaata tgacagggc cgatgcggt tatacgtgg 
      gctcgctac atttcaaca taactgaag acatgctca 
      ggagcatat ttgaaccct ttggcaaaa tagacaata 
      ttcagttaa ttatggatc cagaaacgg ggcgatcaa 
      agggttacg ggttcattg cgtttcgta attgcgagg 
      acgcgaaaa aagctttgg aacaattga acgggtttg 
      aattagcgg gtagaccaa tgaaggtcg gaaatgtaa 
      cagaaagac tagatttac aacagcaag gtccttcaa 
      tacttgatt ccgacgagt tggatcgct cgggtatag 
      atttaggtg caacgggac gtttgcaat tgatgttca 
      aattggccg agggggcgg gaatgcaag taccacaag 
      ctgcagcta acgctctga gtatagcga caggacagc 
      cagtggttc cacaagtcc aaacgaatt caacaccac 
      ctatcgcaa cgcaatgtt tcatgttga gtaacatgt 
      ttgacccgg caacggagt caacgcaga cttgggacg 
      ttgagatta gagatgacg taatagaag agtgtaaca 
      aacacgggg gtgtgctac acgtctatg tagataagg 
      ggtcgccac agggaaatg tttacgtta agtgtccgt 
      ctatagcca cggcagtgg cgtccgtta attcgctgc 
      acggccgat ggtttgcag gacgggtta tcacagcgg 
      cgtacgtcc ctctattga attatcatt cgttgtttc 
      ctgatgcta tgaatgcag cgcaattgc tattgccat 
      ccagtagga agtaatcat gtatttttc attgtcctg 
      tatcattat tatttgtaa taaattgca tcgtgtatg 
      taggcattt tgtaaccat cacaacaaa gtgacttag 
      gactaggga gaaagggtt attttaagt cagactcga 
      gaatgattg tagagacta cgatttatt gtgagaatg 
      tgagaaata aagtgactt gtttaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY