Status
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Gene information
General information
TC014173 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 2784396..2787388
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014173
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10079

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with unidentifyable segments - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    gena triplet bristle sometimes duplicated - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014173 RA
      MDDVNFAHR ARKRIKEVK KKARPELST KESWIQCNY 
      ANNFKKFWD FTDNCERID ESKVSQREF ISNYELPYK 
      PVVITGTQV GWKANEKWT LEKLVKKYR NQKFKCGED 
      NDGYNVKLK MKYYVHYML NSKDDSPLY IFDSNFGEH 
      RRRKKLLED YEVPYYFRD DLFKYAGEN QRPPYRWFV 
      MGPARSGTG IHIDPLGTS AWNALIQGH KRWCLFPTH 
      TPKELLKVT SSVGGKQLD EAITWFNFI YPKTKEPSW 
      PADCQPIEI LQKPGETVF VPGGWWHVV LNLDTTIAV 
      TQNFCSRTN FPVVWHKTA RGRPKFSRK WLKALVKHE 
      PDLAKLAER INIEQPSGM ASDSSSDSS SSSSSDSDS 
      TDSEDGDSG QESITAKKK RRKSDNESE RNRKISRTN 
      SSECATDFN SNHRASISC LTADDHCL
    CDS sequence
    • >TC014173 RA
      atggatgat gttaatttt gcacacagg gcacgcaaa 
      cgtataaaa gaagtaaag aagaaagct cgaccagaa 
      ctaagcacc aaagagtcc tggatccag tgcaactat 
      gcgaacaat tttaagaaa ttctgggac ttcactgac 
      aactgcgaa cgcattgac gaatcaaaa gtttcccag 
      cgcgaattt atatcaaat tatgagctt ccctacaag 
      ccagtcgtt atcactgga actcaggta ggctggaaa 
      gccaacgag aaatggacg ctagagaag ttggtcaag 
      aaatacagg aaccagaag ttcaaatgt ggcgaggac 
      aatgacggt tacaacgtt aagctgaag atgaagtac 
      tatgtacat tacatgctc aattctaaa gacgatagc 
      cccttatat atttttgat agcaatttc ggcgagcac 
      cgacgccgg aaaaaatta ttagaagac tatgaagtg 
      ccctactac ttcagagac gacttattc aaatacgca 
      ggggaaaac caaaggccg ccctacagg tggttcgtc 
      atggggccc gcgaggtcc gggaccggg atccacatc 
      gacccgctc ggcaccagc gcctggaac gccctcatc 
      caaggacac aagcgatgg tgtttgttc ccgacgcac 
      acgccgaaa gagttgctt aaagtgacg agttctgtg 
      ggcggcaag cagttggac gaagcgatc acttggttc 
      aacttcatc tacccgaaa accaaagaa ccatcgtgg 
      cccgctgac tgccagccg atcgagatt ctgcaaaag 
      ccgggcgaa accgtgttc gtgccgggc ggttggtgg 
      catgtggtc ctcaacctg gacaccacg atcgccgtg 
      acgcagaac ttctgcagc cggacgaat ttccccgtc 
      gtttggcac aagacggcc agaggcagg ccgaagttc 
      tcgcggaaa tggctgaaa gctttggtt aagcacgag 
      ccggacctg gccaagctg gccgagagg atcaacatc 
      gagcagccc tcggggatg gcgtccgac agctcaagc 
      gactcgtcg tcgagctcc agctcggac tcggacagc 
      accgactcg gaggacggc gacagtggc caggagtcc 
      atcactgcg aagaagaag aggaggaag tctgataac 
      gagtcggaa cgcaatagg aaaatttct cgcaccaat 
      agctctgaa tgcgccacg gacttcaat tcaaatcat 
      cgagcttcc atatcatgt ctgactgcg gatgaccac 
      tgtctgtga
    mRNA sequence
    • >TC014173 RA
      tgccacctc cggtggagt tttgactgt tgctgattc 
      agtttcaac gttttgaaa taattttct gtttttttc 
      accaaaaat ttttcaaat tgctttagt tcaaggaat 
      ggatgatgt taattttgc acacagggc acgcaaacg 
      tataaaaga agtaaagaa gaaagctcg accagaact 
      aagcaccaa agagtcctg gatccagtg caactatgc 
      gaacaattt taagaaatt ctgggactt cactgacaa 
      ctgcgaacg cattgacga atcaaaagt ttcccagcg 
      cgaatttat atcaaatta tgagcttcc ctacaagcc 
      agtcgttat cactggaac tcaggtagg ctggaaagc 
      caacgagaa atggacgct agagaagtt ggtcaagaa 
      atacaggaa ccagaagtt caaatgtgg cgaggacaa 
      tgacggtta caacgttaa gctgaagat gaagtacta 
      tgtacatta catgctcaa ttctaaaga cgatagccc 
      cttatatat ttttgatag caatttcgg cgagcaccg 
      acgccggaa aaaattatt agaagacta tgaagtgcc 
      ctactactt cagagacga cttattcaa atacgcagg 
      ggaaaacca aaggccgcc ctacaggtg gttcgtcat 
      ggggcccgc gaggtccgg gaccgggat ccacatcga 
      cccgctcgg caccagcgc ctggaacgc cctcatcca 
      aggacacaa gcgatggtg tttgttccc gacgcacac 
      gccgaaaga gttgcttaa agtgacgag ttctgtggg 
      cggcaagca gttggacga agcgatcac ttggttcaa 
      cttcatcta cccgaaaac caaagaacc atcgtggcc 
      cgctgactg ccagccgat cgagattct gcaaaagcc 
      gggcgaaac cgtgttcgt gccgggcgg ttggtggca 
      tgtggtcct caacctgga caccacgat cgccgtgac 
      gcagaactt ctgcagccg gacgaattt ccccgtcgt 
      ttggcacaa gacggccag aggcaggcc gaagttctc 
      gcggaaatg gctgaaagc tttggttaa gcacgagcc 
      ggacctggc caagctggc cgagaggat caacatcga 
      gcagccctc ggggatggc gtccgacag ctcaagcga 
      ctcgtcgtc gagctccag ctcggactc ggacagcac 
      cgactcgga ggacggcga cagtggcca ggagtccat 
      cactgcgaa gaagaagag gaggaagtc tgataacga 
      gtcggaacg caataggaa aatttctcg caccaatag 
      ctctgaatg cgccacgga cttcaattc aaatcatcg 
      agcttccat atcatgtct gactgcgga tgaccactg 
      tctgtgagg caagattga tatctaaat tctcttact 
      tgtttagtt accattctt tagtccgtt ctcttagag 
      gtttattca atcattgaa tctagttaa gttgtttcc 
      gagtattta acgttgtat tatttattg tctcatcga 
      aggaaaatt ggtaaatta tacacgatt tgtaaattg 
      tgccaattt ttgtaaaag gttgcgact cctgcagta 
      tttagggtg acttaattt accaatttt ttcaagggt 
      tattgttta aagtgcttt gactatttt gtattatat 
      ctcagctcc ctcaaagat tatgtatat attccttgt 
      tgggtcatt tgtgatcta agctagtgc ctgaatttg 
      tatattggt atttggtag aaactgcaa atgtataca 
      cgaaatgag gctcaatat tttatcggt tattttcac 
      acaggctga gtatatcag atgaggata aattgactt 
      gctagaggt gcccagaag ctaaagtac ccaccttgg 
      gaaacttcc atactaaac tatgactac tcaagttct 
      cagattttt aagagaaaa actattttt ttaaacatt 
      gatgtttat ttttgttta taattaaca tttaataga 
      aacactaac gatacgttg gagctacaa aacaataaa 
      aattaaata actgaagtt cagttagac taccattat 
      tattattaa ctgctgtac atttaataa aaaaataga 
      gaaagtcaa ttattatat ttcacgttt ttgagcttt 
      tttctttag ttgagataa atattaatt taagttaat 
      tctgccaaa aaagtatca aagaatttc tttaatgga 
      taacgcgaa ccttcactg ccaggtgac cactgtagc 
      ttctgcccc actcaagtg tcaaattgt cgcgctggt 
      gtacttggt gccacttag gcagtagga attatttca 
      gtcgcaaga catttgttc aggcactta tcgtttgtt 
      gaatactat ttatttgca gttcagtta ctgggctac 
      atacttatt tgaggacat gactaagtt tgacttatt 
      ttgttacat accacgaaa tggtgttca agcccatta 
      ccaccaagt attactcag aaaacagtg aacaactat 
      gtaacaagt gtaatcaac tttctgtgt gtgcctttt 
      gtcttttaa atatttcta aataaatat taattaata 
      cg
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