Status
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Gene information
General information
TC013368 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10422638..10425355
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013368
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10078

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013368 RA
      MSLSRSASA TPKPSKGTI SGGDSEQRF PEIKTMEDV 
      IAQFFNQIV QFVIEDMVV VEKKEEALE EKKESLVKD 
      YKELKAEDL VDAEVKWAS GSKVALSKK SSSRGLKSG 
      RPGSSFASN LDAIKEEIS EASRSKTST TASSSLQDK 
      IEDEYEAKI IFTNGNVYE GTISKQTMN GKGRYIWND 
      GTVYEGDFV DGFPTGKGE MTLPDLSHY EGEFNQGLF 
      HGHGFLNIV STPTFYSGE WRNGCKHGQ GWLLYEPED 
      WYEGGWAYN LKDGFGVRF YKNGAKYRG SWREGKYDG 
      CGTLIWENN DFYDGEWKK GLMHGQGEY IWGAFYNEA 
      FAFPPQTIY RGSWEKGKR CGEGIMHFG DDTGLRLQG 
      IWDQDFKHG DGLLICGNG RVVQQNLLF QFDKPKPKT 
      DSRWSIRNF YYSHILDVP VFTAPEYVD VGFYVEIIL 
      SKLNDIPDE KFNLLRTYE EKCIRFTIT RNLPKLKDL 
      YKDYATMAA KIKLDYDPI MIRLFLWQL YKDMGLSKR 
      ISITRADEI LAENPRSCL VSIHDPFEP IYFWQFLMS 
      IIGIALSTG SLEETYDKS IDGGVCAFI VQKFLDEVI 
      FSKAPDHQS TILMNYLDL APIKAVYAL YKKIGEPHS 
      ARTFLKQTC TKKGESPVA CHLELRLQK ARPLFGTNI 
      VPLDEHITY EKERVADFD EKIRHHWTN LFDFRHLGP 
      RNVMRCLAT ACPLVMDDN LVVNVDYPL TFLEFYETL 
      LTCIFMVLE LELKKEQKI LSQSITPAP SVPSSPKVK 
      TVESKKKLP KEKKQKKKK KK
    CDS sequence
    • >TC013368 RA
      atgtcactg tcgaggtcc gcttcggcg acccccaag 
      ccctccaag gggaccatc agtggggga gacagcgag 
      cagcgcttc cccgaaatc aaaacgatg gaagatgtc 
      atcgcgcag ttctttaac cagatcgtg caattcgtc 
      attgaggac atggttgtg gtggagaag aaagaagag 
      gcgcttgag gagaagaag gagagtttg gtcaaggac 
      tataaggag ttgaaagct gaggatttg gtcgatgcc 
      gaggtcaag tgggcctcg gggtccaag gttgcgctg 
      agcaagaag agcagctca agagggctc aagtcgggc 
      aggcctggg agcagtttc gcctcgaat ttggacgct 
      attaaggag gaaatctcc gaagcgagt cgatctaaa 
      acctccacg accgcgtcg tcttccctg caagacaaa 
      atcgaggac gagtacgaa gctaaaatc atcttcaca 
      aatggcaac gtctacgaa gggacaatt tctaaacaa 
      accatgaac ggcaaaggc cgatacatt tggaatgat 
      gggactgtg tatgagggt gattttgta gacggtttt 
      ccgaccggc aaaggcgaa atgaccctg cccgaccta 
      agccactac gagggggag ttcaaccag ggcctattc 
      cacggccac ggtttcctc aacattgtc tcaaccccc 
      actttttac agcggagag tggcgaaat gggtgcaag 
      cacgggcaa gggtggctg ctctacgag cccgaggac 
      tggtacgaa gggggttgg gcatacaac ttaaaggac 
      ggattcggt gtgagattt tacaaaaac ggggcaaag 
      tatcgtggt tcttggcgg gagggaaaa tacgacggg 
      tgtggaacc ctaatttgg gaaaacaat gacttttat 
      gacggagag tggaaaaaa gggttgatg cacggccag 
      ggcgaatac atctggggg gctttctac aacgaagcg 
      ttcgccttc ccccctcag acaatttat cgcgggtcg 
      tgggagaag ggcaaacgt tgtggcgag gggattatg 
      cacttcggg gatgatacg ggattaagg ctgcaaggg 
      atttgggat caggatttt aagcacggc gatgggtta 
      ttaatttgt ggcaacggt cgggtggtg cagcaaaat 
      ttgctcttc cagttcgac aaacctaaa cctaaaact 
      gactcgcgg tggtccatt cgcaatttt tactacagc 
      cacatttta gacgtccct gtttttacg gcccccgaa 
      tatgtagat gtgggattt tacgtcgag ataattttg 
      tcgaaactg aacgatatt cccgacgag aaattcaac 
      ttgttgagg acgtacgaa gagaagtgc atccggttt 
      acgatcact cggaattta ccaaaactg aaggatttg 
      tacaaggat tatgccaca atggctgcg aaaattaaa 
      ctcgactat gacccgatt atgatcagg ttgttttta 
      tggcaatta tacaaagac atgggatta tcgaagcgc 
      atctctata actcgagcc gatgaaatt ctggccgaa 
      aatcccaga agttgcctt gttagtatc cacgacccg 
      ttcgagccg atttatttt tggcaattt ttaatgtca 
      atcattggg attgctctg tccacgggg tctttagag 
      gagacctac gacaagtca attgacggg ggcgtttgt 
      gcgttcatt gtgcaaaaa tttctggac gaagttatt 
      ttttcaaag gctccagac caccaaagt acaattctc 
      atgaattat ctcgatttg gccccaatt aaagccgtt 
      tatgcgctt tataaaaaa atcggggaa ccccactca 
      gctcggact tttttaaaa caaacttgc acgaaaaaa 
      ggggagtcc ccggtagcg tgccatttg gaactgagg 
      ttgcaaaaa gcgcggcct ttgttcgga accaatata 
      gtgccactc gatgagcac atcacgtac gaaaaagaa 
      cgagtcgcc gatttcgat gaaaagata agacaccac 
      tggacaaat ctcttcgat ttccgtcat ttgggcccc 
      agaaatgtg atgcgttgt ttggctacg gcttgtcct 
      ctagtcatg gacgataat ctcgtggtt aacgtcgac 
      tatccactt acgtttctc gagttttac gaaactttg 
      ctaacttgt atttttatg gttttggaa ttggaattg 
      aaaaaagaa cagaagatt ctatcacaa agtattacc 
      cccgcaccc agtgttcca tcgtcgccg aaagtcaaa 
      actgttgag tcgaaaaag aaacttcca aaagaaaag 
      aaacaaaag aaaaagaag aagaaataa
    mRNA sequence
    • >TC013368 RA
      catattact catcgagat acaagaagt gacaaatgt 
      cactgtcga ggtccgctt cggcgaccc ccaagccct 
      ccaagggga ccatcagtg ggggagaca gcgagcagc 
      gcttccccg aaatcaaaa cgatggaag atgtcatcg 
      cgcagttct ttaaccaga tcgtgcaat tcgtcattg 
      aggacatgg ttgtggtgg agaagaaag aagaggcgc 
      ttgaggaga agaaggaga gtttggtca aggactata 
      aggagttga aagctgagg atttggtcg atgccgagg 
      tcaagtggg cctcggggt ccaaggttg cgctgagca 
      agaagagca gctcaagag ggctcaagt cgggcaggc 
      ctgggagca gtttcgcct cgaatttgg acgctatta 
      aggaggaaa tctccgaag cgagtcgat ctaaaacct 
      ccacgaccg cgtcgtctt ccctgcaag acaaaatcg 
      aggacgagt acgaagcta aaatcatct tcacaaatg 
      gcaacgtct acgaaggga caatttcta aacaaacca 
      tgaacggca aaggccgat acatttgga atgatggga 
      ctgtgtatg agggtgatt ttgtagacg gttttccga 
      ccggcaaag gcgaaatga ccctgcccg acctaagcc 
      actacgagg gggagttca accagggcc tattccacg 
      gccacggtt tcctcaaca ttgtctcaa cccccactt 
      tttacagcg gagagtggc gaaatgggt gcaagcacg 
      ggcaagggt ggctgctct acgagcccg aggactggt 
      acgaagggg gttgggcat acaacttaa aggacggat 
      tcggtgtga gattttaca aaaacgggg caaagtatc 
      gtggttctt ggcgggagg gaaaatacg acgggtgtg 
      gaaccctaa tttgggaaa acaatgact tttatgacg 
      gagagtgga aaaaagggt tgatgcacg gccagggcg 
      aatacatct ggggggctt tctacaacg aagcgttcg 
      ccttccccc ctcagacaa tttatcgcg ggtcgtggg 
      agaagggca aacgttgtg gcgagggga ttatgcact 
      tcggggatg atacgggat taaggctgc aagggattt 
      gggatcagg attttaagc acggcgatg ggttattaa 
      tttgtggca acggtcggg tggtgcagc aaaatttgc 
      tcttccagt tcgacaaac ctaaaccta aaactgact 
      cgcggtggt ccattcgca atttttact acagccaca 
      ttttagacg tccctgttt ttacggccc ccgaatatg 
      tagatgtgg gattttacg tcgagataa ttttgtcga 
      aactgaacg atattcccg acgagaaat tcaacttgt 
      tgaggacgt acgaagaga agtgcatcc ggtttacga 
      tcactcgga atttaccaa aactgaagg atttgtaca 
      aggattatg ccacaatgg ctgcgaaaa ttaaactcg 
      actatgacc cgattatga tcaggttgt ttttatggc 
      aattataca aagacatgg gattatcga agcgcatct 
      ctataactc gagccgatg aaattctgg ccgaaaatc 
      ccagaagtt gccttgtta gtatccacg acccgttcg 
      agccgattt atttttggc aatttttaa tgtcaatca 
      ttgggattg ctctgtcca cggggtctt tagaggaga 
      cctacgaca agtcaattg acgggggcg tttgtgcgt 
      tcattgtgc aaaaatttc tggacgaag ttatttttt 
      caaaggctc cagaccacc aaagtacaa ttctcatga 
      attatctcg atttggccc caattaaag ccgtttatg 
      cgctttata aaaaaatcg gggaacccc actcagctc 
      ggacttttt taaaacaaa cttgcacga aaaaagggg 
      agtccccgg tagcgtgcc atttggaac tgaggttgc 
      aaaaagcgc ggcctttgt tcggaacca atatagtgc 
      cactcgatg agcacatca cgtacgaaa aagaacgag 
      tcgccgatt tcgatgaaa agataagac accactgga 
      caaatctct tcgatttcc gtcatttgg gccccagaa 
      atgtgatgc gttgtttgg ctacggctt gtcctctag 
      tcatggacg ataatctcg tggttaacg tcgactatc 
      cacttacgt ttctcgagt tttacgaaa ctttgctaa 
      cttgtattt ttatggttt tggaattgg aattgaaaa 
      aagaacaga agattctat cacaaagta ttacccccg 
      cacccagtg ttccatcgt cgccgaaag tcaaaactg 
      ttgagtcga aaaagaaac ttccaaaag aaaagaaac 
      aaaagaaaa agaagaaga aataaaata ttaaagtga 
      tcgagttat gtaaccaaa accattgtt ttgtacaca 
      gttcaaggg gac
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