Status
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Gene information
General information
TC013377 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10311933..10315395
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013377
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10075

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (3 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013377 RA
      MLPLVEYGK EFCQKLTQI KLSRIVIMV FIILILTPL 
FTHFYLSNI ERSSNHITH NAHSHIDLP EDLNAMKEP 
DLRIGVEEL IRIKGSVLS ELRDMERKR QKLLLEIQS 
YTKNIEELK QELVHQQTN LNRLKISVE QAEVAQREA 
LQQNTPDLA LPKRLTADE IPPILPSLP PSQIKFCRM 
FSCFDHSRC SLTSGFPVY LYDPDQFPV MNDGWDVDG 
FLKTTLKQT LGYNPHLTN NPKEACVFV VLVGEALKD 
VDDVSENVG NHLNLPPLD SNALKQLPY WGGDGRNHV 
LLNLARRDL TAVSGDVFS SVDTGKAIL VQSTFSSQV 
FRPGFDLIV SPVLGPPGG DVWQECPQM LPARRKYLL 
TFQGEVKSN NNRTPNPSH GDDADLDRE HAELDKFVI 
EHLKDLAKT NTADKFLFE FECVPASDD NVNIGPQDW 
ALCGTDSSR RTVLRDSTF VLIFAPTFP DLVTTTLLQ 
ARIYEALRC GAIPVILGG DQIQLAYDE VIQWRRVAI 
FLPRARITE LHFLLRAIP DEDVLLMRR QGRLVWERY 
LASVQSTLD TIVAVLRDR LNIPPIPID TVAAISVFN 
ETFQPIQVT VPVETEQEE SLGPLEPPY NSPAFRRNY 
SLGLTQGHE MWNDWGDPF RLYPALPTD PLLPSDAKF 
MGSGRGFRP IGQGQGGAG KEFSEALGG NSPREQFTV 
LLLTYEREQ VLLDSIARL RGLPYLNSV VIVWNSPRP 
PSAELRWPD IGAPVHVVK AARNSLNNR FLPLDNLQT 
EAILSVDDD AHLRHDEIL FGFRVWREH RERIVGFPG 
RYHAWDINT QNSWLYNSN YSCELSMVL TGAAFLHRH 
YLHLYWKWL PQAIRDKVD EYMNCEDIA MNFLVSHIT 
RLPPVKVTS RWTFRCPGC PQSLSEDDT HFQERHKCI 
NFFSQVFGY TPLLNTQYR ADSILFKTR IPHDKQKCF 
KFI
    CDS sequence
    • >TC013377 RA
      atgctgcct ctcgtcgaa tacggcaaa gagttttgc 
caaaagctc acccaaatc aaactgtct cgaatcgtc 
atcatggtc ttcataatt ttaattcta acgcctctt 
tttacccac ttttacctc tcaaacatt gagcggagc 
tcgaaccac atcacacac aacgctcac tcacatatt 
gacctccct gaagacctc aacgccatg aaagagcca 
gatctgcga atcggagtt gaggaatta atccgaatt 
aaaggctca gtcctgagc gaattgcga gacatggaa 
cgcaaacgg caaaaactc ctcttggaa atccagtct 
tacacaaaa aacatcgaa gaattgaaa caagaactc 
gtccaccaa caaaccaat ctcaaccgc ttgaaaatc 
tcagtggaa caagctgag gtagcccaa cgggaagcc 
ctccaacaa aacaccccc gacctagcg ctcccgaaa 
cgcctaacc gccgacgaa atcccccca atcctcccg 
tcactcccg ccgtcccag atcaaattc tgccggatg 
ttttcctgt tttgaccac tcacggtgc tccctaact 
tccgggttc ccggtctat ttatacgac ccggaccag 
ttcccggtc atgaacgac ggttgggac gtggacggt 
ttcctcaaa acgacactg aagcagacg ctggggtac 
aacccccac ctgacgaac aaccccaaa gaggcgtgc 
gttttcgtg gttttggtc ggggaggcg ttgaaagac 
gtggatgac gtcagcgag aacgtgggg aatcacctg 
aatcttccc ccgttggac tccaacgcc ttgaagcag 
ctgccgtat tggggcggc gacggccgc aaccacgtt 
ctgcttaat ttagcccgc agggacctc acggcggtc 
tctggggac gttttttcc tcagtcgat actggcaaa 
gccatccta gtccagagc actttctcg tcccaggtg 
ttcaggcct ggctttgat ttgatcgtg tcgccggtt 
ttggggccc ccggggggc gatgtgtgg caagagtgc 
ccccaaatg ttgcccgcg cgccgcaaa tacctccta 
acgtttcag ggcgaagtt aaaagtaac aataaccgg 
acgcctaac ccctcacat ggggacgac gccgacttg 
gatcgggag cacgccgaa ctcgataaa ttcgtaatc 
gaacattta aaagatctg gcgaaaacc aacactgcc 
gacaagttc ctgtttgaa ttcgagtgc gtcccggct 
agcgacgat aacgttaat attggaccc caagactgg 
gccttgtgt ggcactgat agctcacgc aggaccgtg 
ttaagggac tccactttt gtgcttatt ttcgcccca 
acgttcccc gatttggtc acaacgacg ctgttacag 
gcccggatt tacgaggct ttgcgttgt ggggccatc 
cctgtgatt ttagggggc gaccagatc caattggct 
tatgacgaa gtgatccag tggcgtaga gtagcaatt 
tttttgcca agagcgcga attacagaa ttacatttt 
ttgttgcgg gcaatcccc gacgaagac gttttgttg 
atgagacgt caggggcgg ctcgtgtgg gagcgctac 
ttggcctca gtccaaagc acacttgat acgatcgtc 
gcagtgctc cgtgatagg ctgaatatt ccaccaatc 
cccatagac acagtcgcg gccatctct gtgtttaat 
gaaactttc cagccgatt caagtgaca gttccggtg 
gaaacggaa caagaggag agtttgggg cccctggag 
cccccttat aatagtccc gcgtttcgc cgaaactac 
agtctgggg ttaacacaa ggacacgag atgtggaac 
gactggggg gaccctttc cggttgtat ccagccctc 
ccgactgac cccctcctg ccctcagac gccaagttt 
atggggtca gggcggggc ttccgtccg attggtcaa 
ggtcaaggg ggcgcaggc aaggaattt tcggaagcg 
ttaggcggt aacagcccc cgggagcaa ttcacagtg 
ctcctacta acctacgaa cgcgagcaa gtcctgctt 
gattcaatt gccaggctt cgggggctg ccgtacctc 
aactcggtc gtaatcgtg tggaactct ccacggccc 
cctagcgcg gagctccgc tggcccgac attggggcc 
cccgttcat gtcgtcaaa gccgcccgt aattcgctc 
aataaccgc tttctcccc cttgataat ctccagacc 
gaggcgatt ttgtcggtc gatgacgat gcccattta 
cgacacgat gaaattctt ttcgggttt agggtttgg 
agggagcac agggaacgc attgtgggg ttccccgga 
cgttaccac gcgtgggac atcaacacg cagaacagc 
tggttgtac aactcgaac tacagctgc gagttgagt 
atggttttg acgggggcg gcgtttctg cacagacat 
tacttgcat ttgtactgg aaatggttg cctcaagcg 
atcagagat aaagttgac gagtacatg aattgtgaa 
gatatagcg atgaatttc ctggtaagt catataacg 
aggttgccg ccggttaaa gtcacttcg aggtggact 
ttccggtgc cccggctgt ccgcaaagt ctctccgaa 
gatgacacg cattttcag gagagacac aaatgtatt 
aattttttc tcgcaagtg tttgggtac acgccgttg 
cttaatacc cagtataga gccgactcg attctgttc 
aaaacgcgt ataccacac gataagcaa aagtgtttc 
aagtttatt tag
    mRNA sequence
    • >TC013377 RA
      gctcgcacc attcagttt gagaaaaac tgggagtga 
gtcggaaag ttcatttta aaaaattgt ttacaattt 
cttgtgtgt ataaagaga ggataaaca agaattacg 
tacaccagc catgggtta acgtcctcg tgttaaaaa 
aaaccgcct taaagccta aaaatgcat ttgtgcaaa 
ttagtgagg ttatctccc ctgtaatcc atcggctgt 
ttttccgaa aacacacgc cgacaatgc tgcctctcg 
tcgaatacg gcaaagagt tttgccaaa agctcaccc 
aaatcaaac tgtctcgaa tcgtcatca tggtcttca 
taattttaa ttctaacgc ctcttttta cccactttt 
acctctcaa acattgagc ggagctcga accacatca 
cacacaacg ctcactcac atattgacc tccctgaag 
acctcaacg ccatgaaag agccagatc tgcgaatcg 
gagttgagg aattaatcc gaattaaag gctcagtcc 
tgagcgaat tgcgagaca tggaacgca aacggcaaa 
aactcctct tggaaatcc agtcttaca caaaaaaca 
tcgaagaat tgaaacaag aactcgtcc accaacaaa 
ccaatctca accgcttga aaatctcag tggaacaag 
ctgaggtag cccaacggg aagccctcc aacaaaaca 
cccccgacc tagcgctcc cgaaacgcc taaccgccg 
acgaaatcc ccccaatcc tcccgtcac tcccgccgt 
cccagatca aattctgcc ggatgtttt cctgttttg 
accactcac ggtgctccc taacttccg ggttcccgg 
tctatttat acgacccgg accagttcc cggtcatga 
acgacggtt gggacgtgg acggtttcc tcaaaacga 
cactgaagc agacgctgg ggtacaacc cccacctga 
cgaacaacc ccaaagagg cgtgcgttt tcgtggttt 
tggtcgggg aggcgttga aagacgtgg atgacgtca 
gcgagaacg tggggaatc acctgaatc ttcccccgt 
tggactcca acgccttga agcagctgc cgtattggg 
gcggcgacg gccgcaacc acgttctgc ttaatttag 
cccgcaggg acctcacgg cggtctctg gggacgttt 
tttcctcag tcgatactg gcaaagcca tcctagtcc 
agagcactt tctcgtccc aggtgttca ggcctggct 
ttgatttga tcgtgtcgc cggttttgg ggcccccgg 
ggggcgatg tgtggcaag agtgccccc aaatgttgc 
ccgcgcgcc gcaaatacc tcctaacgt ttcagggcg 
aagttaaaa gtaacaata accggacgc ctaacccct 
cacatgggg acgacgccg acttggatc gggagcacg 
ccgaactcg ataaattcg taatcgaac atttaaaag 
atctggcga aaaccaaca ctgccgaca agttcctgt 
ttgaattcg agtgcgtcc cggctagcg acgataacg 
ttaatattg gaccccaag actgggcct tgtgtggca 
ctgatagct cacgcagga ccgtgttaa gggactcca 
cttttgtgc ttattttcg ccccaacgt tccccgatt 
tggtcacaa cgacgctgt tacaggccc ggatttacg 
aggctttgc gttgtgggg ccatccctg tgattttag 
ggggcgacc agatccaat tggcttatg acgaagtga 
tccagtggc gtagagtag caatttttt tgccaagag 
cgcgaatta cagaattac attttttgt tgcgggcaa 
tccccgacg aagacgttt tgttgatga gacgtcagg 
ggcggctcg tgtgggagc gctacttgg cctcagtcc 
aaagcacac ttgatacga tcgtcgcag tgctccgtg 
ataggctga atattccac caatcccca tagacacag 
tcgcggcca tctctgtgt ttaatgaaa ctttccagc 
cgattcaag tgacagttc cggtggaaa cggaacaag 
aggagagtt tggggcccc tggagcccc cttataata 
gtcccgcgt ttcgccgaa actacagtc tggggttaa 
cacaaggac acgagatgt ggaacgact ggggggacc 
ctttccggt tgtatccag ccctcccga ctgaccccc 
tcctgccct cagacgcca agtttatgg ggtcagggc 
ggggcttcc gtccgattg gtcaaggtc aagggggcg 
caggcaagg aattttcgg aagcgttag gcggtaaca 
gcccccggg agcaattca cagtgctcc tactaacct 
acgaacgcg agcaagtcc tgcttgatt caattgcca 
ggcttcggg ggctgccgt acctcaact cggtcgtaa 
tcgtgtgga actctccac ggcccccta gcgcggagc 
tccgctggc ccgacattg gggcccccg ttcatgtcg 
tcaaagccg cccgtaatt cgctcaata accgctttc 
tcccccttg ataatctcc agaccgagg cgattttgt 
cggtcgatg acgatgccc atttacgac acgatgaaa 
ttcttttcg ggtttaggg tttggaggg agcacaggg 
aacgcattg tggggttcc ccggacgtt accacgcgt 
gggacatca acacgcaga acagctggt tgtacaact 
cgaactaca gctgcgagt tgagtatgg ttttgacgg 
gggcggcgt ttctgcaca gacattact tgcatttgt 
actggaaat ggttgcctc aagcgatca gagataaag 
ttgacgagt acatgaatt gtgaagata tagcgatga 
atttcctgg taagtcata taacgaggt tgccgccgg 
ttaaagtca cttcgaggt ggactttcc ggtgccccg 
gctgtccgc aaagtctct ccgaagatg acacgcatt 
ttcaggaga gacacaaat gtattaatt ttttctcgc 
aagtgtttg ggtacacgc cgttgctta atacccagt 
atagagccg actcgattc tgttcaaaa cgcgtatac 
cacacgata agcaaaagt gtttcaagt ttatttagg 
cagttgtgt tttatacgg actttttat ttattttaa 
tttaatgac cggaaatgc aggccacta atttaataa 
ataattaat ttggttttg ttacactgg caaacccat 
ctatttaaa ataattgtg attttaaaa actagcacc 
gtgggcagt tttatagcg aaattttat aggaatctg 
taaccgaga acatttaaa tgtcagtga tatttaaaa 
atgtaacgt gccattctc tttttaatt gtaaatacc 
tctctattt acttttttt ttattgtac atacgtcat 
tttttatta aacgattat attatta

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY