Status
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Gene information
General information
TC013377 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10311933..10315395
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013377
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10075

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013377 RA
      MLPLVEYGK EFCQKLTQI KLSRIVIMV FIILILTPL 
      FTHFYLSNI ERSSNHITH NAHSHIDLP EDLNAMKEP 
      DLRIGVEEL IRIKGSVLS ELRDMERKR QKLLLEIQS 
      YTKNIEELK QELVHQQTN LNRLKISVE QAEVAQREA 
      LQQNTPDLA LPKRLTADE IPPILPSLP PSQIKFCRM 
      FSCFDHSRC SLTSGFPVY LYDPDQFPV MNDGWDVDG 
      FLKTTLKQT LGYNPHLTN NPKEACVFV VLVGEALKD 
      VDDVSENVG NHLNLPPLD SNALKQLPY WGGDGRNHV 
      LLNLARRDL TAVSGDVFS SVDTGKAIL VQSTFSSQV 
      FRPGFDLIV SPVLGPPGG DVWQECPQM LPARRKYLL 
      TFQGEVKSN NNRTPNPSH GDDADLDRE HAELDKFVI 
      EHLKDLAKT NTADKFLFE FECVPASDD NVNIGPQDW 
      ALCGTDSSR RTVLRDSTF VLIFAPTFP DLVTTTLLQ 
      ARIYEALRC GAIPVILGG DQIQLAYDE VIQWRRVAI 
      FLPRARITE LHFLLRAIP DEDVLLMRR QGRLVWERY 
      LASVQSTLD TIVAVLRDR LNIPPIPID TVAAISVFN 
      ETFQPIQVT VPVETEQEE SLGPLEPPY NSPAFRRNY 
      SLGLTQGHE MWNDWGDPF RLYPALPTD PLLPSDAKF 
      MGSGRGFRP IGQGQGGAG KEFSEALGG NSPREQFTV 
      LLLTYEREQ VLLDSIARL RGLPYLNSV VIVWNSPRP 
      PSAELRWPD IGAPVHVVK AARNSLNNR FLPLDNLQT 
      EAILSVDDD AHLRHDEIL FGFRVWREH RERIVGFPG 
      RYHAWDINT QNSWLYNSN YSCELSMVL TGAAFLHRH 
      YLHLYWKWL PQAIRDKVD EYMNCEDIA MNFLVSHIT 
      RLPPVKVTS RWTFRCPGC PQSLSEDDT HFQERHKCI 
      NFFSQVFGY TPLLNTQYR ADSILFKTR IPHDKQKCF 
      KFI
    CDS sequence
    • >TC013377 RA
      atgctgcct ctcgtcgaa tacggcaaa gagttttgc 
      caaaagctc acccaaatc aaactgtct cgaatcgtc 
      atcatggtc ttcataatt ttaattcta acgcctctt 
      tttacccac ttttacctc tcaaacatt gagcggagc 
      tcgaaccac atcacacac aacgctcac tcacatatt 
      gacctccct gaagacctc aacgccatg aaagagcca 
      gatctgcga atcggagtt gaggaatta atccgaatt 
      aaaggctca gtcctgagc gaattgcga gacatggaa 
      cgcaaacgg caaaaactc ctcttggaa atccagtct 
      tacacaaaa aacatcgaa gaattgaaa caagaactc 
      gtccaccaa caaaccaat ctcaaccgc ttgaaaatc 
      tcagtggaa caagctgag gtagcccaa cgggaagcc 
      ctccaacaa aacaccccc gacctagcg ctcccgaaa 
      cgcctaacc gccgacgaa atcccccca atcctcccg 
      tcactcccg ccgtcccag atcaaattc tgccggatg 
      ttttcctgt tttgaccac tcacggtgc tccctaact 
      tccgggttc ccggtctat ttatacgac ccggaccag 
      ttcccggtc atgaacgac ggttgggac gtggacggt 
      ttcctcaaa acgacactg aagcagacg ctggggtac 
      aacccccac ctgacgaac aaccccaaa gaggcgtgc 
      gttttcgtg gttttggtc ggggaggcg ttgaaagac 
      gtggatgac gtcagcgag aacgtgggg aatcacctg 
      aatcttccc ccgttggac tccaacgcc ttgaagcag 
      ctgccgtat tggggcggc gacggccgc aaccacgtt 
      ctgcttaat ttagcccgc agggacctc acggcggtc 
      tctggggac gttttttcc tcagtcgat actggcaaa 
      gccatccta gtccagagc actttctcg tcccaggtg 
      ttcaggcct ggctttgat ttgatcgtg tcgccggtt 
      ttggggccc ccggggggc gatgtgtgg caagagtgc 
      ccccaaatg ttgcccgcg cgccgcaaa tacctccta 
      acgtttcag ggcgaagtt aaaagtaac aataaccgg 
      acgcctaac ccctcacat ggggacgac gccgacttg 
      gatcgggag cacgccgaa ctcgataaa ttcgtaatc 
      gaacattta aaagatctg gcgaaaacc aacactgcc 
      gacaagttc ctgtttgaa ttcgagtgc gtcccggct 
      agcgacgat aacgttaat attggaccc caagactgg 
      gccttgtgt ggcactgat agctcacgc aggaccgtg 
      ttaagggac tccactttt gtgcttatt ttcgcccca 
      acgttcccc gatttggtc acaacgacg ctgttacag 
      gcccggatt tacgaggct ttgcgttgt ggggccatc 
      cctgtgatt ttagggggc gaccagatc caattggct 
      tatgacgaa gtgatccag tggcgtaga gtagcaatt 
      tttttgcca agagcgcga attacagaa ttacatttt 
      ttgttgcgg gcaatcccc gacgaagac gttttgttg 
      atgagacgt caggggcgg ctcgtgtgg gagcgctac 
      ttggcctca gtccaaagc acacttgat acgatcgtc 
      gcagtgctc cgtgatagg ctgaatatt ccaccaatc 
      cccatagac acagtcgcg gccatctct gtgtttaat 
      gaaactttc cagccgatt caagtgaca gttccggtg 
      gaaacggaa caagaggag agtttgggg cccctggag 
      cccccttat aatagtccc gcgtttcgc cgaaactac 
      agtctgggg ttaacacaa ggacacgag atgtggaac 
      gactggggg gaccctttc cggttgtat ccagccctc 
      ccgactgac cccctcctg ccctcagac gccaagttt 
      atggggtca gggcggggc ttccgtccg attggtcaa 
      ggtcaaggg ggcgcaggc aaggaattt tcggaagcg 
      ttaggcggt aacagcccc cgggagcaa ttcacagtg 
      ctcctacta acctacgaa cgcgagcaa gtcctgctt 
      gattcaatt gccaggctt cgggggctg ccgtacctc 
      aactcggtc gtaatcgtg tggaactct ccacggccc 
      cctagcgcg gagctccgc tggcccgac attggggcc 
      cccgttcat gtcgtcaaa gccgcccgt aattcgctc 
      aataaccgc tttctcccc cttgataat ctccagacc 
      gaggcgatt ttgtcggtc gatgacgat gcccattta 
      cgacacgat gaaattctt ttcgggttt agggtttgg 
      agggagcac agggaacgc attgtgggg ttccccgga 
      cgttaccac gcgtgggac atcaacacg cagaacagc 
      tggttgtac aactcgaac tacagctgc gagttgagt 
      atggttttg acgggggcg gcgtttctg cacagacat 
      tacttgcat ttgtactgg aaatggttg cctcaagcg 
      atcagagat aaagttgac gagtacatg aattgtgaa 
      gatatagcg atgaatttc ctggtaagt catataacg 
      aggttgccg ccggttaaa gtcacttcg aggtggact 
      ttccggtgc cccggctgt ccgcaaagt ctctccgaa 
      gatgacacg cattttcag gagagacac aaatgtatt 
      aattttttc tcgcaagtg tttgggtac acgccgttg 
      cttaatacc cagtataga gccgactcg attctgttc 
      aaaacgcgt ataccacac gataagcaa aagtgtttc 
      aagtttatt tag
    mRNA sequence
    • >TC013377 RA
      gctcgcacc attcagttt gagaaaaac tgggagtga 
      gtcggaaag ttcatttta aaaaattgt ttacaattt 
      cttgtgtgt ataaagaga ggataaaca agaattacg 
      tacaccagc catgggtta acgtcctcg tgttaaaaa 
      aaaccgcct taaagccta aaaatgcat ttgtgcaaa 
      ttagtgagg ttatctccc ctgtaatcc atcggctgt 
      ttttccgaa aacacacgc cgacaatgc tgcctctcg 
      tcgaatacg gcaaagagt tttgccaaa agctcaccc 
      aaatcaaac tgtctcgaa tcgtcatca tggtcttca 
      taattttaa ttctaacgc ctcttttta cccactttt 
      acctctcaa acattgagc ggagctcga accacatca 
      cacacaacg ctcactcac atattgacc tccctgaag 
      acctcaacg ccatgaaag agccagatc tgcgaatcg 
      gagttgagg aattaatcc gaattaaag gctcagtcc 
      tgagcgaat tgcgagaca tggaacgca aacggcaaa 
      aactcctct tggaaatcc agtcttaca caaaaaaca 
      tcgaagaat tgaaacaag aactcgtcc accaacaaa 
      ccaatctca accgcttga aaatctcag tggaacaag 
      ctgaggtag cccaacggg aagccctcc aacaaaaca 
      cccccgacc tagcgctcc cgaaacgcc taaccgccg 
      acgaaatcc ccccaatcc tcccgtcac tcccgccgt 
      cccagatca aattctgcc ggatgtttt cctgttttg 
      accactcac ggtgctccc taacttccg ggttcccgg 
      tctatttat acgacccgg accagttcc cggtcatga 
      acgacggtt gggacgtgg acggtttcc tcaaaacga 
      cactgaagc agacgctgg ggtacaacc cccacctga 
      cgaacaacc ccaaagagg cgtgcgttt tcgtggttt 
      tggtcgggg aggcgttga aagacgtgg atgacgtca 
      gcgagaacg tggggaatc acctgaatc ttcccccgt 
      tggactcca acgccttga agcagctgc cgtattggg 
      gcggcgacg gccgcaacc acgttctgc ttaatttag 
      cccgcaggg acctcacgg cggtctctg gggacgttt 
      tttcctcag tcgatactg gcaaagcca tcctagtcc 
      agagcactt tctcgtccc aggtgttca ggcctggct 
      ttgatttga tcgtgtcgc cggttttgg ggcccccgg 
      ggggcgatg tgtggcaag agtgccccc aaatgttgc 
      ccgcgcgcc gcaaatacc tcctaacgt ttcagggcg 
      aagttaaaa gtaacaata accggacgc ctaacccct 
      cacatgggg acgacgccg acttggatc gggagcacg 
      ccgaactcg ataaattcg taatcgaac atttaaaag 
      atctggcga aaaccaaca ctgccgaca agttcctgt 
      ttgaattcg agtgcgtcc cggctagcg acgataacg 
      ttaatattg gaccccaag actgggcct tgtgtggca 
      ctgatagct cacgcagga ccgtgttaa gggactcca 
      cttttgtgc ttattttcg ccccaacgt tccccgatt 
      tggtcacaa cgacgctgt tacaggccc ggatttacg 
      aggctttgc gttgtgggg ccatccctg tgattttag 
      ggggcgacc agatccaat tggcttatg acgaagtga 
      tccagtggc gtagagtag caatttttt tgccaagag 
      cgcgaatta cagaattac attttttgt tgcgggcaa 
      tccccgacg aagacgttt tgttgatga gacgtcagg 
      ggcggctcg tgtgggagc gctacttgg cctcagtcc 
      aaagcacac ttgatacga tcgtcgcag tgctccgtg 
      ataggctga atattccac caatcccca tagacacag 
      tcgcggcca tctctgtgt ttaatgaaa ctttccagc 
      cgattcaag tgacagttc cggtggaaa cggaacaag 
      aggagagtt tggggcccc tggagcccc cttataata 
      gtcccgcgt ttcgccgaa actacagtc tggggttaa 
      cacaaggac acgagatgt ggaacgact ggggggacc 
      ctttccggt tgtatccag ccctcccga ctgaccccc 
      tcctgccct cagacgcca agtttatgg ggtcagggc 
      ggggcttcc gtccgattg gtcaaggtc aagggggcg 
      caggcaagg aattttcgg aagcgttag gcggtaaca 
      gcccccggg agcaattca cagtgctcc tactaacct 
      acgaacgcg agcaagtcc tgcttgatt caattgcca 
      ggcttcggg ggctgccgt acctcaact cggtcgtaa 
      tcgtgtgga actctccac ggcccccta gcgcggagc 
      tccgctggc ccgacattg gggcccccg ttcatgtcg 
      tcaaagccg cccgtaatt cgctcaata accgctttc 
      tcccccttg ataatctcc agaccgagg cgattttgt 
      cggtcgatg acgatgccc atttacgac acgatgaaa 
      ttcttttcg ggtttaggg tttggaggg agcacaggg 
      aacgcattg tggggttcc ccggacgtt accacgcgt 
      gggacatca acacgcaga acagctggt tgtacaact 
      cgaactaca gctgcgagt tgagtatgg ttttgacgg 
      gggcggcgt ttctgcaca gacattact tgcatttgt 
      actggaaat ggttgcctc aagcgatca gagataaag 
      ttgacgagt acatgaatt gtgaagata tagcgatga 
      atttcctgg taagtcata taacgaggt tgccgccgg 
      ttaaagtca cttcgaggt ggactttcc ggtgccccg 
      gctgtccgc aaagtctct ccgaagatg acacgcatt 
      ttcaggaga gacacaaat gtattaatt ttttctcgc 
      aagtgtttg ggtacacgc cgttgctta atacccagt 
      atagagccg actcgattc tgttcaaaa cgcgtatac 
      cacacgata agcaaaagt gtttcaagt ttatttagg 
      cagttgtgt tttatacgg actttttat ttattttaa 
      tttaatgac cggaaatgc aggccacta atttaataa 
      ataattaat ttggttttg ttacactgg caaacccat 
      ctatttaaa ataattgtg attttaaaa actagcacc 
      gtgggcagt tttatagcg aaattttat aggaatctg 
      taaccgaga acatttaaa tgtcagtga tatttaaaa 
      atgtaacgt gccattctc tttttaatt gtaaatacc 
      tctctattt acttttttt ttattgtac atacgtcat 
      tttttatta aacgattat attatta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY