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Gene information
General information
TC014423 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10270227..10271191
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014423
    iBeetle screen iB_10073

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014423 RA
      MVEVNNTEA ECSKKQKKP PKRGIVYLS 
      TIPPYMNVT KIREVFGQF GKIGRVYLQ 
      LAETEGKNE KKSKRKVAR KFTEGWVEF 
      ERKSVAKRV AQLLNNTQV STRKKSKQF 
      DCIWSIKYL SNFKWTHLH ERLAYEKAA 
      RRQKLRAEI QLAKKKTNF FTANLDKKK 
      KGKDVVNKE LENIETNGE TPMDEESQE 
      DRTEFLQSL FS
    CDS sequence
    • >TC014423 RA
      atggttgaa gtaaacaac acggaggca gaatgttca 
      aaaaaacag aaaaagcca cccaaacgg ggcatagtg 
      tacttgtcg acgatcccg ccgtacatg aatgtgacg 
      aagattcgt gaggtgttc ggtcagttt gggaaaatc 
      ggtcgggtt tacctccaa cttgcagag acagagggt 
      aaaaatgag aagaagtcg aagaggaaa gtggcgcgg 
      aaattcacg gagggctgg gtggaattt gagcgcaag 
      tcggttgca aaaagggtg gcccaattg ttaaataat 
      actcaagtc agtacacgc aaaaaatcc aaacagttt 
      gactgtatc tggagcatt aagtatttg tcaaatttc 
      aaatggacg cacttgcat gaaagactg gcctatgag 
      aaagccgct aggaggcag aaactgagg gctgaaata 
      cagttggcc aagaagaaa accaacttc ttcaccgcc 
      aatttggat aagaagaag aaagggaaa gacgtggtt 
      aataaagag ttggaaaat attgagacc aatggggag 
      actccgatg gatgaggag agtcaagag gataggaca 
      gaattttta caatcgtta ttttcgtga
    mRNA sequence
    • >TC014423 RA
      cgatttggc tatatcagt tcgggatgc ctggatgca 
      actcaccac aacaaatat ttaacacga ctgtggcga 
      cctctctaa aactctaat taaaaaaaa tcaaattac 
      cgcattaaa atcaataaa aacgtaaca gggatgttt 
      gcgtaaacc gtttattac tcttttaat aacaaagag 
      taattaaaa gtgcgcggg cgtgttgga aattccgct 
      ctcccaaac acctgttgg taacttcac ttttacagg 
      gttggtgca acttgacac tcaaccacg tgctaacct 
      agaaaatgg ttgaagtaa acaacacgg aggcagaat 
      gttcaaaaa aacagaaaa agccaccca aacggggca 
      tagtgtact tgtcgacga tcccgccgt acatgaatg 
      tgacgaaga ttcgtgagg tgttcggtc agtttggga 
      aaatcggtc gggtttacc tccaacttg cagagacag 
      agggtaaaa atgagaaga agtcgaaga ggaaagtgg 
      cgcggaaat tcacggagg gctgggtgg aatttgagc 
      gcaagtcgg ttgcaaaaa gggtggccc aattgttaa 
      ataatactc aagtcagta cacgcaaaa aatccaaac 
      agtttgact gtatctgga gcattaagt atttgtcaa 
      atttcaaat ggacgcact tgcatgaaa gactggcct 
      atgagaaag ccgctagga ggcagaaac tgagggctg 
      aaatacagt tggccaaga agaaaacca acttcttca 
      ccgccaatt tggataaga agaagaaag ggaaagacg 
      tggttaata aagagttgg aaaatattg agaccaatg 
      gggagactc cgatggatg aggagagtc aagaggata 
      ggacagaat ttttacaat cgttatttt cgtgataat 
      ttattaagt tttgtaatt ta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY