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Gene information
General information
TC013382 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10232961..10235848
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013382
    iBeetle screen iB_10071

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013382 RA
      MKETFILPD FQPLLEVSG LNCDKVVII SIMGKSPYN 
      SMGMKAKSI GRVFTAGCK SDDECTIEG FYDEENQII 
      YLHLHSLLD TDLLIKHYS SLVERCNNE RKCDDLLVI 
      NDEIKSSFA RALLLLFHV SHIMILSHP GWTFDTNWI 
      QYFKAVDTL SQKKLNKIS EVLKNVDGL SPDWVSNGR 
      FCTPRVIFY FEQCPKGSY NIKKVEHNL EDRIYHILK 
      KTRIISTYG SSLFAIPMN EEFVYISTE APSTDRLGD 
      AVRGLIQDC QPGGAMQVQ PPFCSQPYS ERHFLKFLQ 
      VHIKQARTK GFNDTGSSS RHQLSQPAH FELPILKHW 
      LESTKAIYE VIMKKGLIS SLCTDTRFS EQRCLKVLP 
      LALARYQEN LPPHYAHSE HDRRLSMAL ALFNAQARG 
      PVFQQYVTK LKADCKAHW ENGRQQCEA ASMTGNPCK 
      LPKHQSDQE HMSGFIYKA VCDCGRKIG PREDPYNAK 
      QANHLFYQA ISRECICSK LERISFPVF QPSIKEYKA 
      ATLKETEEA LSVLSDRSN ELTPENERG LVRQPSTTE 
      YLPGMLTLS SPPGLLPVY SSWSLVCLG PSSLYSHNL 
      GLSESHHPG FLNSTNYLL PWDVTVYSK AKMNWVSKY 
      PARGRRGRP TGASPQFSV KVFIGVEYE CSSGHRFML 
      AAPDKILKA TPGSIVKDT GHKIAESDM PLYYPCMCR 
      ANKLAQLMR LHVVTPKAP VHCTLDPKV QPAPGAPIF 
      ISTVERPTK LTQSAYWVM RLPYVYVAD KEHYPQNLG 
      AKLLQGVFG VTEIE
    CDS sequence
    • >TC013382 RA
      atgaaagaa acatttatc ttaccggat tttcagcct 
      cttttggag gtgagtggt ttaaattgc gacaaagtc 
      gtaattatc tcaatcatg ggcaagtcc ccttacaac 
      tcaatgggg atgaaagcc aagtcaatt gggcgagtt 
      tttacagca gggtgtaaa agcgacgat gaatgtacg 
      attgagggt ttctatgat gaggaaaac cagatcatt 
      tatctccac ctccacagt cttttagac acagattta 
      ttaattaaa cactactca agtctcgtt gagcggtgc 
      aataatgaa cgaaagtgc gacgattta cttgtaatt 
      aacgacgaa attaagagt agttttgct cgcgcgctc 
      ttactattg tttcacgtg agtcacatt atgattttg 
      tctcatcct gggtggact tttgataca aactggatc 
      cagtatttc aaggctgtg gatacgtta agtcaaaaa 
      aaactcaat aaaataagc gaagttttg aaaaacgtt 
      gacggtttg agccccgat tgggtttcc aatggccgg 
      ttttgcacg ccccgtgtt attttctac tttgagcag 
      tgcccgaag ggctcatac aacatcaag aaggttgaa 
      cacaacctt gaagatcgc atttaccac attttgaaa 
      aagacacgg ataatctca acctatggt tcgtcgctt 
      tttgcaatt ccgatgaat gaggaattc gtttacata 
      tcgacggaa gcgccaagc actgaccgg ttaggggat 
      gccgtcaga ggcttgatt caggattgc cagcctggc 
      ggcgctatg caagtccag cccccattt tgcagccag 
      ccttactcc gaacgccac tttttgaaa ttcttgcaa 
      gtgcacata aaacaggcc cggactaag ggttttaac 
      gacacgggg agctccagt cggcatcag ttgtcacaa 
      ccggcccac tttgagtta cccatactg aaacattgg 
      cttgagagc accaaggcc atatacgaa gtgataatg 
      aaaaaaggc ttgatctcg tcgctatgc accgacacg 
      agattttcc gaacagagg tgtctcaaa gtcctgcct 
      ttggcgcta gcgcggtac caggaaaac ttgccgcca 
      cattacgcg cactcagag cacgacagg cgcctcagc 
      atggccttg gccctcttt aacgctcag gccagaggc 
      cctgtgttc cagcaatac gtcacgaaa ttgaaggcc 
      gactgcaag gcgcattgg gagaacggg agacagcag 
      tgcgaggcg gcttccatg accgggaac ccgtgcaaa 
      ttgcccaaa catcagtca gatcaggag cacatgagc 
      gggtttatt tacaaagct gtttgcgac tgcggccgg 
      aaaatcggc ccgcgtgag gacccctac aacgccaaa 
      caagccaat cacctcttc taccaagcg atcagtcgc 
      gaatgcatt tgctcgaaa ttggagcgc atttccttc 
      cccgttttc caaccctcg atcaaagaa tacaaagca 
      gcgactttg aaagagacc gaagaggcc ctttcggtc 
      ctctccgac cgctccaat gaactcacc cccgaaaac 
      gaaaggggg ctggtccgc cagccgagc acgaccgaa 
      tatttaccg ggaatgcta accctgagt tcgccccct 
      ggcctcctc ccggtctac tccagctgg agtctcgtc 
      tgcctcggc ccctcctcg ctttacagc cacaatttg 
      gggctgtcg gagtcgcac catcccggt tttttgaac 
      tctactaac tacttgctc ccgtgggac gtgacagtg 
      tatagcaag gcgaaaatg aattgggtt agtaagtac 
      ccggcgagg gggcgtcgg ggacgtccg acgggggct 
      tcgccccag ttttcggtc aaggttttc attggagtg 
      gagtatgag tgttcgtcg ggacataga tttatgttg 
      gcagccccg gataaaatt ctcaaggcc acgccgggg 
      agcattgtg aaggacact gggcataaa atagcagag 
      tcggatatg cccttgtat tatccgtgc atgtgtcgt 
      gccaataag ttggcacaa ctgatgcga ctgcatgtt 
      gttaccccc aaggctcca gtgcattgc actctggac 
      ccaaaggtg cagcccgca cccggggcc cccattttc 
      atcagtacg gtggaacgg cccaccaaa ctgacccaa 
      tcggcctat tgggtcatg cggttgcct tacgtctat 
      gttgcggat aaggaacac tacccgcaa aatttagga 
      gcaaagcta ctacagggc gtttttgga gtaacagaa 
      atagaataa
    mRNA sequence
    • >TC013382 RA
      gctagctcc atgggcgca gtgactgtc tggtcgact 
      gtaatgttc atcatagat tctattatc tattatgat 
      ttttggtgt tgttttcac cgatatcct gtcatcagt 
      tttaacccc aaaaaacaa agatgaaag aaacattta 
      tcttaccgg attttcagc ctcttttgg aggtgagtg 
      gtttaaatt gcgacaaag tcgtaatta tctcaatca 
      tgggcaagt ccccttaca actcaatgg ggatgaaag 
      ccaagtcaa ttgggcgag tttttacag cagggtgta 
      aaagcgacg atgaatgta cgattgagg gtttctatg 
      atgaggaaa accagatca tttatctcc acctccaca 
      gtcttttag acacagatt tattaatta aacactact 
      caagtctcg ttgagcggt gcaataatg aacgaaagt 
      gcgacgatt tacttgtaa ttaacgacg aaattaaga 
      gtagttttg ctcgcgcgc tcttactat tgtttcacg 
      tgagtcaca ttatgattt tgtctcatc ctgggtgga 
      cttttgata caaactgga tccagtatt tcaaggctg 
      tggatacgt taagtcaaa aaaaactca ataaaataa 
      gcgaagttt tgaaaaacg ttgacggtt tgagccccg 
      attgggttt ccaatggcc ggttttgca cgccccgtg 
      ttattttct actttgagc agtgcccga agggctcat 
      acaacatca agaaggttg aacacaacc ttgaagatc 
      gcatttacc acattttga aaaagacac ggataatct 
      caacctatg gttcgtcgc tttttgcaa ttccgatga 
      atgaggaat tcgtttaca tatcgacgg aagcgccaa 
      gcactgacc ggttagggg atgccgtca gaggcttga 
      ttcaggatt gccagcctg gcggcgcta tgcaagtcc 
      agcccccat tttgcagcc agccttact ccgaacgcc 
      actttttga aattcttgc aagtgcaca taaaacagg 
      cccggacta agggtttta acgacacgg ggagctcca 
      gtcggcatc agttgtcac aaccggccc actttgagt 
      tacccatac tgaaacatt ggcttgaga gcaccaagg 
      ccatatacg aagtgataa tgaaaaaag gcttgatct 
      cgtcgctat gcaccgaca cgagatttt ccgaacaga 
      ggtgtctca aagtcctgc ctttggcgc tagcgcggt 
      accaggaaa acttgccgc cacattacg cgcactcag 
      agcacgaca ggcgcctca gcatggcct tggccctct 
      ttaacgctc aggccagag gccctgtgt tccagcaat 
      acgtcacga aattgaagg ccgactgca aggcgcatt 
      gggagaacg ggagacagc agtgcgagg cggcttcca 
      tgaccggga acccgtgca aattgccca aacatcagt 
      cagatcagg agcacatga gcgggttta tttacaaag 
      ctgtttgcg actgcggcc ggaaaatcg gcccgcgtg 
      aggacccct acaacgcca aacaagcca atcacctct 
      tctaccaag cgatcagtc gcgaatgca tttgctcga 
      aattggagc gcatttcct tccccgttt tccaaccct 
      cgatcaaag aatacaaag cagcgactt tgaaagaga 
      ccgaagagg ccctttcgg tcctctccg accgctcca 
      atgaactca cccccgaaa acgaaaggg ggctggtcc 
      gccagccga gcacgaccg aatatttac cgggaatgc 
      taaccctga gttcgcccc ctggcctcc tcccggtct 
      actccagct ggagtctcg tctgcctcg gcccctcct 
      cgctttaca gccacaatt tggggctgt cggagtcgc 
      accatcccg gttttttga actctacta actacttgc 
      tcccgtggg acgtgacag tgtatagca aggcgaaaa 
      tgaattggg ttagtaagt acccggcga gggggcgtc 
      ggggacgtc cgacggggg cttcgcccc agttttcgg 
      tcaaggttt tcattggag tggagtatg agtgttcgt 
      cgggacata gatttatgt tggcagccc cggataaaa 
      ttctcaagg ccacgccgg ggagcattg tgaaggaca 
      ctgggcata aaatagcag agtcggata tgcccttgt 
      attatccgt gcatgtgtc gtgccaata agttggcac 
      aactgatgc gactgcatg ttgttaccc ccaaggctc 
      cagtgcatt gcactctgg acccaaagg tgcagcccg 
      cacccgggg cccccattt tcatcagta cggtggaac 
      ggcccacca aactgaccc aatcggcct attgggtca 
      tgcggttgc cttacgtct atgttgcgg ataaggaac 
      actacccgc aaaatttag gagcaaagc tactacagg 
      gcgtttttg gagtaacag aaatagaat aaatggttt 
      tcaatgact ggaattttt ttatgcgag tttgaaaga 
      cccga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY