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Gene information
General information
TC013390 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10193538..10198880
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013390
    iBeetle screen iB_10069

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Fertility
    ovary robustness decreased - Number of animals showing the phenotype: 4
    Comment: dissecting the ovaries is almost impossible. Very fragile
    mature egg not present - Number of animals showing the phenotype: 4
    tropharium structure irregular - Number of animals showing the phenotype: 1

    egg chamber potentially number decreased - Number of animals showing the phenotype: 2
    previtellogenic egg chamber position irregular - Number of animals showing the phenotype: 1

    previtellogenic egg chamber potentially number decreased - Number of animals showing the phenotype: 1
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    head capsule size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    head structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    head segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    prothorax segment partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs femur potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax segmentation/division irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen cuticle structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013390 RA
      MISHSIFAI FLALISSAI SETNDDILV FTVASEPTD 
      GFQRYLSSA HHYHIAPTV LGFGQEWKG GSDIKNRPG 
      GGWKINLLK TALEPHKDD PTKIILFTD GYDVIFTDT 
      LDAILRKFK ETKARVLFG AESSCWPDV QLAPKYPQV 
      TEGKRFLNS GLYMGYAPD LWQVLTFDV IEDTDDDQL 
      FFTKAYLDE DLRKKVGFK LDHKSEIFQ NLNGAVSEV 
      ELFEVKAKE GPEEYKIQN VLYHTVPLI LHGNGPSKL 
      SLNYLGNYL ANSWNSVEG CVRCKEGQF DLKNKRANE 
      MSLVLLAIF VEFNTPFLE EMLSKVYSQ EYPKHRIDL 
      FIHNAMKFH SKHITDFIE KHGSEYRSV KDIKPDDGT 
      TEWAARDLS LAQCLSKNC DIYFSVDSV AHLDNPHTL 
      RLLIEQNRT VVAPLLPRP GKAWSNFWG DLTKEGFYA 
      RSNDYMDIV HNDKRGLWN VPFIANCYA INATLLKKF 
      DETKLNFDR DNWDADMAF CANLRDLDV FMYVSNRVD 
      FGHLVNPET FDITRVEPE MYQIFDNEQ DWEARFIHP 
      EYPENFNPE KTSLQPCPD VYWFPIVSP RFCTSLINM 
      MENFGKWSD GSNKDPRLE GGYEAVPTR DIHMNQVGW 
      EKHWLEFLR KYVRPLQEH VFLGYFHDP PRSLMNFVV 
      RYKPDEQPS LRPHHDSST YTINIALNQ RGVDYEGGG 
      CRFIRYNCS VVDTKLGWL LIHPGRLTH YHEGLKVTK 
      GIRYIMIAF VDP
    CDS sequence
    • >TC013390 RA
      atgattagt cacagtatt ttcgccata tttcttgcg 
      ttaatttcg agtgccatt agcgaaaca aatgacgac 
      attctagtc ttcacagtc gctagcgag ccaaccgac 
      ggcttccag cgctacctc tcctccgcc catcactac 
      cacatcgcc cccacggtc ctgggtttc ggccaggaa 
      tggaaaggg ggctccgat attaaaaac cgccccgga 
      ggcggctgg aaaatcaac ctccttaaa actgccctc 
      gaaccacac aaagacgac cccacaaaa ataatcctt 
      ttcactgat ggctacgat gtcatcttc accgacact 
      ctcgatgcc attttgcgc aaatttaaa gaaaccaaa 
      gcccgggtt ttattcgga gccgaaagc tcctgttgg 
      cctgacgta caactggcc ccaaaatac ccccaagtg 
      actgaagga aaacgattc ctaaattcg gggttgtac 
      atgggctac gcccccgac ttgtggcaa gtgctcact 
      tttgacgtg attgaggac acggacgac gatcaactg 
      tttttcact aaagcgtac ctcgatgag gatttgaga 
      aaaaaagtt gggttcaag ttggaccac aaaagtgag 
      attttccaa aatctgaat ggggctgtt agtgaagtc 
      gagttgttt gaggttaaa gcgaaggaa ggtcctgag 
      gaatataaa attcaaaat gttttatat cacactgta 
      ccgttgatt ttgcacggg aatggccct tcgaaatta 
      tccctaaat tatttaggg aactacttg gcgaattcg 
      tggaattcg gtggaaggg tgcgtccgg tgcaaagag 
      ggccagttt gacttgaaa aacaaaagg gcaaacgaa 
      atgtctctt gtccttctt gcgattttt gtagaattt 
      aataccccc ttcttggag gaaatgttg agtaaggtg 
      tactcacag gagtacccc aagcaccgt atcgatctc 
      tttatccac aatgcgatg aaatttcat tcaaaacac 
      ataaccgac tttatcgaa aaacacggt tctgagtac 
      cgctcagtc aaagatatc aagccggac gatggtacc 
      accgaatgg gccgcacgt gacttatct ttggcccaa 
      tgtttgtcc aaaaactgc gacatttac ttttccgtt 
      gattcggtc gcccatttg gacaacccg cacactctt 
      cggctttta atcgagcag aaccgcacc gtggtagcc 
      ccccttttg ccccgtcct ggcaaggcc tggagcaac 
      ttttggggg gacttgacc aaggaaggt ttctatgcc 
      cggtcaaac gactacatg gatatagtc cacaacgat 
      aagagaggg ctatggaac gtcccgttc atagccaac 
      tgctacgcc atcaatgcc acgctcttg aagaagttt 
      gatgagacg aagttgaac ttcgatcgg gataactgg 
      gacgcggac atggccttt tgtgccaat ttacgcgac 
      ttggacgtc ttcatgtac gtctcgaac cgcgtcgat 
      ttcgggcat ttggtcaat ccggaaacc ttcgatatc 
      actcgggtc gaaccggaa atgtaccaa atcttcgac 
      aatgaacag gattgggag gccaggttc atccatccc 
      gaatatcct gaaaacttc aaccccgaa aagacaagt 
      ctacagcct tgtccagac gtttactgg ttccccatc 
      gtgtcgcca cgtttttgt accagcttg atcaacatg 
      atggaaaat ttcggaaaa tggtccgat ggtagcaat 
      aaggatccc cggctcgaa ggcggctat gaggccgtc 
      ccgacgcgt gatatccac atgaatcaa gtcggttgg 
      gaaaagcat tggctggag ttcctccgg aagtacgtg 
      aggcctttg caggagcac gttttcctc gggtatttc 
      catgacccg ccacgatct ttaatgaat ttcgtggtt 
      cggtataaa ccggacgaa caaccctcc ttaaggcca 
      catcacgac agctcgacc tacaccatc aacatagcc 
      ttgaatcag agaggggtt gattacgaa ggcgggggt 
      tgtaggttc attaggtac aactgcagc gttgttgat 
      accaaactt ggatggttg ttgatacac ccgggaagg 
      ctcactcac taccatgaa gggttgaag gtcacaaaa 
      gggattaga tatatcatg attgctttc gttgaccca 
      tag
    mRNA sequence
    • >TC013390 RA
      taacaattt tagcgacag caaactcca acgaacaat 
      gcatcaaag actcggaaa catgtggag aaaagccag 
      aaagtgcat ttttgcatc accagaagt ccacgacag 
      atgcaggtg agcaaaatt tggttctat ttcattcat 
      ttacgcggc cgctgtttg aattttgtt gcgttcatg 
      ttggcaaaa gacgctcga cgctgccaa cgtaaattt 
      cagtgccat aagaacgtc atggtggtg ggttgtgtt 
      tcgtctcgt tgtaaataa aataccggc aatatttat 
      atttttttt caattaaaa taagaaaac gaacgtaat 
      tatgattag tcacagtat tttcgccat atttcttgc 
      gttaatttc gagtgccat tagcgaaac aaatgacga 
      cattctagt cttcacagt cgctagcga gccaaccga 
      cggcttcca gcgctacct ctcctccgc ccatcacta 
      ccacatcgc ccccacggt cctgggttt cggccagga 
      atggaaagg gggctccga tattaaaaa ccgccccgg 
      aggcggctg gaaaatcaa cctccttaa aactgccct 
      cgaaccaca caaagacga ccccacaaa aataatcct 
      tttcactga tggctacga tgtcatctt caccgacac 
      tctcgatgc cattttgcg caaatttaa agaaaccaa 
      agcccgggt tttattcgg agccgaaag ctcctgttg 
      gcctgacgt acaactggc cccaaaata cccccaagt 
      gactgaagg aaaacgatt cctaaattc ggggttgta 
      catgggcta cgcccccga cttgtggca agtgctcac 
      ttttgacgt gattgagga cacggacga cgatcaact 
      gtttttcac taaagcgta cctcgatga ggatttgag 
      aaaaaaagt tgggttcaa gttggacca caaaagtga 
      gattttcca aaatctgaa tggggctgt tagtgaagt 
      cgagttgtt tgaggttaa agcgaagga aggtcctga 
      ggaatataa aattcaaaa tgttttata tcacactgt 
      accgttgat tttgcacgg gaatggccc ttcgaaatt 
      atccctaaa ttatttagg gaactactt ggcgaattc 
      gtggaattc ggtggaagg gtgcgtccg gtgcaaaga 
      gggccagtt tgacttgaa aaacaaaag ggcaaacga 
      aatgtctct tgtccttct tgcgatttt tgtagaatt 
      taatacccc cttcttgga ggaaatgtt gagtaaggt 
      gtactcaca ggagtaccc caagcaccg tatcgatct 
      ctttatcca caatgcgat gaaatttca ttcaaaaca 
      cataaccga ctttatcga aaaacacgg ttctgagta 
      ccgctcagt caaagatat caagccgga cgatggtac 
      caccgaatg ggccgcacg tgacttatc tttggccca 
      atgtttgtc caaaaactg cgacattta cttttccgt 
      tgattcggt cgcccattt ggacaaccc gcacactct 
      tcggctttt aatcgagca gaaccgcac cgtggtagc 
      ccccctttt gccccgtcc tggcaaggc ctggagcaa 
      cttttgggg ggacttgac caaggaagg tttctatgc 
      ccggtcaaa cgactacat ggatatagt ccacaacga 
      taagagagg gctatggaa cgtcccgtt catagccaa 
      ctgctacgc catcaatgc cacgctctt gaagaagtt 
      tgatgagac gaagttgaa cttcgatcg ggataactg 
      ggacgcgga catggcctt ttgtgccaa tttacgcga 
      cttggacgt cttcatgta cgtctcgaa ccgcgtcga 
      tttcgggca tttggtcaa tccggaaac cttcgatat 
      cactcgggt cgaaccgga aatgtacca aatcttcga 
      caatgaaca ggattggga ggccaggtt catccatcc 
      cgaatatcc tgaaaactt caaccccga aaagacaag 
      tctacagcc ttgtccaga cgtttactg gttccccat 
      cgtgtcgcc acgtttttg taccagctt gatcaacat 
      gatggaaaa tttcggaaa atggtccga tggtagcaa 
      taaggatcc ccggctcga aggcggcta tgaggccgt 
      cccgacgcg tgatatcca catgaatca agtcggttg 
      ggaaaagca ttggctgga gttcctccg gaagtacgt 
      gaggccttt gcaggagca cgttttcct cgggtattt 
      ccatgaccc gccacgatc tttaatgaa tttcgtggt 
      tcggtataa accggacga acaaccctc cttaaggcc 
      acatcacga cagctcgac ctacaccat caacatagc 
      cttgaatca gagaggggt tgattacga aggcggggg 
      ttgtaggtt cattaggta caactgcag cgttgttga 
      taccaaact tggatggtt gttgataca cccgggaag 
      gctcactca ctaccatga agggttgaa ggtcacaaa 
      agggattag atatatcat gattgcttt cgttgaccc 
      atagataag ttcctcgat tgtaataat aacgtacta 
      attgtgtag atctgattt gtcgtcttg tacataact 
      tttgtaacg aatttgttt aataaaagt attttcgca 
      ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY