Status
Please wait ...
Gene information
General information
TC014404 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10159302..10166429
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014404
    iBeetle screen iB_10067

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014404 RA
      MKNYFTCYV LLVLCTRAA FAQFIGQQS QFPNIDQRN 
      QLSPTQQGH ILSDIQQHQ HRFNVFPQT QHFPPQQFG 
      SPQPAAVQP QVFNQQPVF AQPTQFPVL LNQNQQLLQ 
      NPVLVQPQF QAALPTPTV QQPVPVVST IQSIVPTLQ 
      PEVNTRILQ HHTQTSFTP NFSQQQPQP QQQQPQQPP 
      PQQQQSQET IIRSPTTNN VPQNQYNAF TPRPTVDPI 
      VAIEQQIRQ LDPEKHKQR IKELREKQS IIEKHNQFV 
      EKQYEKALK KAQSDHQQW VEEQKEIKK KLYQSVYKP 
      PGNAQVVRY TQSIPRYIY PEEGPLFKL AVEQYYKEH 
      PTTTTTTTT TTTTEAPTT TSKKFLATK KSGGSFLPT 
      ALPTSETKI KTIQTLEDL NLLQKQYKS QQIAKDDLL 
      AQLRLAIGN SPEDEITKN LSSREISLA NGKKVQLIE 
      ATDPKLIPE GKGEEITLP NGEKVQVFQ ANNPSPKPE 
      EITLPNGQK VQVIKTRDP KLLASASSS STVKTQEIT 
      LPNGQKVEV IKTTDPKLV PGATPLEPG SDLEKLVLA 
      QTTTTKPPT AVFDELTKG VLPPGANFE LLKTGASGG 
      LEQVGGLPN QKKVTFVLL EEQEDGTLK VQGVKGSGK 
      DKPEVDVDS ILKRIKNGE IKLPQNAKP TPTTQTKFQ 
      STTPKKDLV TGVTVVPNS FASGEDVSN VPILKTNIG 
      THFVATSTP SSTIYTKIF PSSTTRSTT TSSPTHKVT 
      RNPHILNQG NDLVRQAST TSSGNRNSI IYQTTPKPT 
      SPSYSSTSV KIFQSVSPT VPTTPFYPA PTEAKPPQL 
      ELPEVLKKN GLFAMAKFL RQSGLDTIL NETGPYTIF 
      VPTDKAFRT LLVQLGGPE KAEEKFKDN PRLLSGLLL 
      HHVIPGAFT IGSLQDEMT GVSLAGTQL RVNQYNMHD 
      VEWNDVKVT TINGAKVLD EKQDIEIPQ GIAHAVDRV 
      MFPLPVGDI VQTLQSDRE RRFTSFLRA LFSSGLAET 
      LQGTKTFTL FAPTDKAFA SLSSEDLTK IIADRALAR 
      ELIFRHLIP GTLYTNGMR YYQIKDSLL TDRQLTISK 
      QSGKVKVNN VPLATQNIP ATNGVIHAV DALL
    CDS sequence
    • >TC014404 RA
      atgaagaac tacttcaca tgttatgtt ctcctggtt 
      ctgtgcaca agagcagcg tttgcgcag tttattgga 
      caacaatca caatttcca aacattgat caaagaaat 
      cagctgtca ccgacgcaa caaggtcac atactgagc 
      gatattcag caacatcaa cacaggttt aatgtgttt 
      cctcaaacg caacacttt cctcctcaa cagttcggt 
      tcaccacag cctgcagct gtgcaacca caggtgttc 
      aaccaacaa cctgttttc gctcaacca acacaattt 
      ccagtcttg ttgaaccaa aatcaacaa cttttgcaa 
      aaccccgtt ttggttcaa cctcaattc caagcggct 
      ttaccaaca ccaacagtg caacaacca gttcccgtt 
      gtctcaaca atacagtca attgtacca actttacaa 
      cctgaagtc aacactagg attcttcaa catcacact 
      caaaccagc ttcactcct aatttttcg caacagcag 
      ccacagccg caacagcaa cagccacaa caaccacca 
      ccacaacag caacaatcc caagaaaca attatacga 
      agcccaaca acaaacaat gtaccacaa aatcaatat 
      aatgcgttc accccaagg cccacagtt gacccaatt 
      gtggcaatt gaacaacaa attcgccag ttagatccc 
      gaaaaacac aaacaaaga ataaaagag ttgagggaa 
      aagcagagc attatagag aaacacaat cagtttgtg 
      gaaaaacag tacgagaaa gctttgaag aaagcacaa 
      agcgatcac caacaatgg gtggaggaa caaaaagaa 
      atcaaaaag aaactttac caaagtgta tataaacca 
      ccaggtaat gcacaagtg gtcagatac actcaatca 
      atccctcgg tatatctac cctgaagaa ggcccgctc 
      tttaaatta gcagtggaa caatactac aaagaacac 
      cccacaact acaaccacc acaacaaca actacaaca 
      acagaagct ccaacaacc acgtcaaaa aaatttctc 
      gcaactaaa aaatccggt ggctcgttt ctaccaacc 
      gcacttccg acgtctgaa accaaaatc aaaacaatc 
      caaacccta gaagactta aatcttctt caaaaacag 
      tacaaatcg caacagatc gccaaagac gacttgttg 
      gcccaactt cgtctagcc ataggaaac agtcccgaa 
      gacgaaatc accaaaaat ctttcctcc agagaaatc 
      tccctcgcc aatggcaaa aaagttcaa ctgatcgaa 
      gctactgac ccaaaattg atcccggaa gggaaggga 
      gaagaaatc acattaccc aacggcgaa aaagtccaa 
      gtgtttcaa gcaaacaac ccctcccca aaacccgaa 
      gaaataact ctaccaaac ggccaaaag gtccaagtg 
      atcaaaaca cgagacccc aaactacta gcaagtgcc 
      tcatcctcg tccactgtt aaaacacaa gaaatcact 
      ttgcccaat ggacagaaa gtcgaagtg atcaaaacc 
      acggatcct aaactagta cctggggca actcctcta 
      gaacccgga agcgacttg gaaaaacta gtcttggcc 
      caaactacg acaaccaaa ccaccgaca gcggttttc 
      gacgagtta acaaaaggg gtactacct cctggtgcc 
      aactttgag cttttgaaa accggcgcc agcggcggt 
      ttggagcaa gtcggaggc ttacccaac cagaaaaaa 
      gtcactttc gtactgtta gaggaacaa gaagacggc 
      acgctcaaa gttcaaggg gtcaaaggc agcggaaaa 
      gacaaaccg gaagtcgac gtcgactcg attttgaaa 
      cgaatcaaa aacggcgaa atcaaacta ccccaaaac 
      gccaaacct accccaacc acgcaaact aaatttcaa 
      tcaacgacc cccaaaaaa gacctagtt actggtgtg 
      accgttgtc ccaaattcc ttcgccagt ggcgaagat 
      gtttccaat gttcccatt ttaaaaacc aacattggg 
      acacacttt gtggccacg tcgacgcct tcgagtacc 
      atttacacg aaaattttc cctagtagt actaccaga 
      agtacgacc acgtcaagt cccactcat aaagtgacc 
      cgaaatccg catattctg aaccaaggg aatgaccta 
      gtgaggcaa gcctcgacg acatcgtcg ggaaatcgc 
      aacagtata atataccaa actaccccc aagcccaca 
      agcccgagt tattcctcc acttcggtg aaaatcttc 
      caaagtgtg tctcccacc gttccgaca acacccttc 
      taccccgca cccaccgaa gccaaaccc ccacaacta 
      gaactcccg gaagtcctc aaaaagaac ggcttgttc 
      gccatggct aagtttctc cgccagtca ggtctagac 
      accatcctg aacgaaacc gggccttac acaatcttc 
      gtcccgact gacaaagcc ttcaggacc cttctagtc 
      cagctggga ggacccgaa aaagccgag gagaaattc 
      aaggacaat ccgcgcctc ctcagcggg ctgcttttg 
      caccacgtg atccccggt gccttcacc ataggctcc 
      ctgcaggac gaaatgacc ggcgtgtct ctggccggc 
      acccaacta agggtcaac cagtacaac atgcacgac 
      gtcgagtgg aacgacgtt aaggttacc acaattaac 
      ggggctaag gtactcgat gagaaacaa gacattgag 
      ataccgcag ggtatagca catgccgtt gatagagtg 
      atgttcccg ctgcctgtg ggcgacatt gtccaaacg 
      ctccagtcg gaccgcgaa aggagattc accagtttc 
      ctgagggcg ttgttctct tccggactt gccgaaacg 
      ttgcaaggc acgaaaacc ttcacgttg ttcgcccca 
      acggacaaa gctttcgcc agtctgtcc agcgaggac 
      ctcaccaag atcatcgcg gacagagcc ctggccaga 
      gagctgatc ttccggcac ctgatcccg ggtactttg 
      tacaccaac gggatgagg tactaccag atcaaggac 
      agtctccta accgaccgc cagctcacc attagcaag 
      cagtcaggc aaagtgaaa gtgaacaat gtccccttg 
      gccacccaa aacatcccc gccacgaac ggggtgatc 
      cacgccgtc gacgcactc ttgtaa
    mRNA sequence
    • >TC014404 RA
      ttcttcatc acatgtggt gttggtgcc ggtactcgg 
      cggtgttgt caagatgaa gaactactt cacatgtta 
      tgttctcct ggttctgtg cacaagagc agcgtttgc 
      gcagtttat tggacaaca atcacaatt tccaaacat 
      tgatcaaag aaatcagct gtcaccgac gcaacaagg 
      tcacatact gagcgatat tcagcaaca tcaacacag 
      gtttaatgt gtttcctca aacgcaaca ctttcctcc 
      tcaacagtt cggttcacc acagcctgc agctgtgca 
      accacaggt gttcaacca acaacctgt tttcgctca 
      accaacaca atttccagt cttgttgaa ccaaaatca 
      acaactttt gcaaaaccc cgttttggt tcaacctca 
      attccaagc ggctttacc aacaccaac agtgcaaca 
      accagttcc cgttgtctc aacaataca gtcaattgt 
      accaacttt acaacctga agtcaacac taggattct 
      tcaacatca cactcaaac cagcttcac tcctaattt 
      ttcgcaaca gcagccaca gccgcaaca gcaacagcc 
      acaacaacc accaccaca acagcaaca atcccaaga 
      aacaattat acgaagccc aacaacaaa caatgtacc 
      acaaaatca atataatgc gttcacccc aaggcccac 
      agttgaccc aattgtggc aattgaaca acaaattcg 
      ccagttaga tcccgaaaa acacaaaca aagaataaa 
      agagttgag ggaaaagca gagcattat agagaaaca 
      caatcagtt tgtggaaaa acagtacga gaaagcttt 
      gaagaaagc acaaagcga tcaccaaca atgggtgga 
      ggaacaaaa agaaatcaa aaagaaact ttaccaaag 
      tgtatataa accaccagg taatgcaca agtggtcag 
      atacactca atcaatccc tcggtatat ctaccctga 
      agaaggccc gctctttaa attagcagt ggaacaata 
      ctacaaaga acaccccac aactacaac caccacaac 
      aacaactac aacaacaga agctccaac aaccacgtc 
      aaaaaaatt tctcgcaac taaaaaatc cggtggctc 
      gtttctacc aaccgcact tccgacgtc tgaaaccaa 
      aatcaaaac aatccaaac cctagaaga cttaaatct 
      tcttcaaaa acagtacaa atcgcaaca gatcgccaa 
      agacgactt gttggccca acttcgtct agccatagg 
      aaacagtcc cgaagacga aatcaccaa aaatctttc 
      ctccagaga aatctccct cgccaatgg caaaaaagt 
      tcaactgat cgaagctac tgacccaaa attgatccc 
      ggaagggaa gggagaaga aatcacatt acccaacgg 
      cgaaaaagt ccaagtgtt tcaagcaaa caacccctc 
      cccaaaacc cgaagaaat aactctacc aaacggcca 
      aaaggtcca agtgatcaa aacacgaga ccccaaact 
      actagcaag tgcctcatc ctcgtccac tgttaaaac 
      acaagaaat cactttgcc caatggaca gaaagtcga 
      agtgatcaa aaccacgga tcctaaact agtacctgg 
      ggcaactcc tctagaacc cggaagcga cttggaaaa 
      actagtctt ggcccaaac tacgacaac caaaccacc 
      gacagcggt tttcgacga gttaacaaa aggggtact 
      acctcctgg tgccaactt tgagctttt gaaaaccgg 
      cgccagcgg cggtttgga gcaagtcgg aggcttacc 
      caaccagaa aaaagtcac tttcgtact gttagagga 
      acaagaaga cggcacgct caaagttca aggggtcaa 
      aggcagcgg aaaagacaa accggaagt cgacgtcga 
      ctcgatttt gaaacgaat caaaaacgg cgaaatcaa 
      actacccca aaacgccaa acctacccc aaccacgca 
      aactaaatt tcaatcaac gacccccaa aaaagacct 
      agttactgg tgtgaccgt tgtcccaaa ttccttcgc 
      cagtggcga agatgtttc caatgttcc cattttaaa 
      aaccaacat tgggacaca ctttgtggc cacgtcgac 
      gccttcgag taccattta cacgaaaat tttccctag 
      tagtactac cagaagtac gaccacgtc aagtcccac 
      tcataaagt gacccgaaa tccgcatat tctgaacca 
      agggaatga cctagtgag gcaagcctc gacgacatc 
      gtcgggaaa tcgcaacag tataatata ccaaactac 
      ccccaagcc cacaagccc gagttattc ctccacttc 
      ggtgaaaat cttccaaag tgtgtctcc caccgttcc 
      gacaacacc cttctaccc cgcacccac cgaagccaa 
      acccccaca actagaact cccggaagt cctcaaaaa 
      gaacggctt gttcgccat ggctaagtt tctccgcca 
      gtcaggtct agacaccat cctgaacga aaccgggcc 
      ttacacaat cttcgtccc gactgacaa agccttcag 
      gacccttct agtccagct gggaggacc cgaaaaagc 
      cgaggagaa attcaagga caatccgcg cctcctcag 
      cgggctgct tttgcacca cgtgatccc cggtgcctt 
      caccatagg ctccctgca ggacgaaat gaccggcgt 
      gtctctggc cggcaccca actaagggt caaccagta 
      caacatgca cgacgtcga gtggaacga cgttaaggt 
      taccacaat taacggggc taaggtact cgatgagaa 
      acaagacat tgagatacc gcagggtat agcacatgc 
      cgttgatag agtgatgtt cccgctgcc tgtgggcga 
      cattgtcca aacgctcca gtcggaccg cgaaaggag 
      attcaccag tttcctgag ggcgttgtt ctcttccgg 
      acttgccga aacgttgca aggcacgaa aaccttcac 
      gttgttcgc cccaacgga caaagcttt cgccagtct 
      gtccagcga ggacctcac caagatcat cgcggacag 
      agccctggc cagagagct gatcttccg gcacctgat 
      cccgggtac tttgtacac caacgggat gaggtacta 
      ccagatcaa ggacagtct cctaaccga ccgccagct 
      caccattag caagcagtc aggcaaagt gaaagtgaa 
      caatgtccc cttggccac ccaaaacat ccccgccac 
      gaacggggt gatccacgc cgtcgacgc actcttgta 
      attagtaag ttattacgt atttatttc attgtcatt 
      attgtatca ttcccacga gccatccca ttgttgtat 
      tgttttatg tactatttt aaaattgtt tgaaaaaag 
      aaagaaaaa ggctgaaat gttcgtcac aacaatgcc 
      aactcttaa attatgaaa aaatgtaat taaccttag 
      ttgtaagtg atcatgtaa tatactatt ttaaataaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY