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Gene information
General information
TC013405 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10050640..10052501
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013405
    iBeetle screen iB_10066

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013405 RA
      MDGMFELYL DGSIEPDDI PHTADPVYI LGKKYNALQ 
      ELDTIRQDI LSKIWFTYR KNFVPIGGD EGLTTDKGW 
      GCMLRCGQM VLAQALVTL HLGRDWVWE PETKDSTYL 
      KILSKFVDK RQAPFSIHQ IAMMGVSEN KEVGQWFGP 
      NTVAQVLKK LVKYDEWSA IEMHIALDN TLIISDIRE 
      LCLSQGSDG CSSGDWKPL LLIVPLRLG LQEINPIYA 
      SGLKKCFQF KQSLGVIGG KPNLALYFI GHVGDEVIY 
      LDPHTTQKS GSVESKETE EEIELDSTY HCKYASRIN 
      ILSMDPSVA VCFFCNTEG EFNDLCHSI KKDLIEPEK 
      QPLFEITYE KPKQWTPTL DDAVEAADF ELSGEYDSE 
      HEFEIL
    CDS sequence
    • >TC013405 RA
      atggacggc atgttcgaa ctataccta gacgggagc 
      attgagccc gatgacatc ccccatact gcagacccc 
      gtctacatc ctaggcaag aagtacaac gcactccaa 
      gaattggac acgatccgc caagacatt ctctcgaaa 
      atctggttc acgtatcgg aaaaatttc gttccaatt 
      gggggcgac gaaggtctc accactgac aagggctgg 
      ggctgcatg ctacgatgc ggccagatg gttctggcg 
      caagccttg gtcacgctg catctgggc cgagactgg 
      gtctgggag cccgaaacc aaagactcc acttatctc 
      aaaatactt agcaaattt gtagacaag agacaagcg 
      ccgttttcc atacaccag attgccatg atgggggtg 
      tcggaaaac aaggaagtg gggcagtgg tttggaccc 
      aacactgtg gcccaagtc ttgaagaag ttggtaaag 
      tatgacgag tggagtgca attgagatg cacatagcg 
      ttagataat acacttatt attagcgat ataagagag 
      ttgtgctta agtcagggg agtgacggt tgtagcagt 
      ggggactgg aagccgttg ctacttatt gtgccttta 
      agattggga ctgcaagaa attaatcct atttatgct 
      agtggatta aagaaatgt tttcagttc aaacaaagc 
      ctgggtgtg atcggtggc aaaccgaac ctggcgctg 
      tatttcata ggacatgta ggagacgaa gtaatatat 
      ctagacccg cacacgacg cagaaatcc ggttccgtg 
      gaaagcaaa gaaacggaa gaagaaatc gagctggat 
      tccacctac cactgtaaa tacgcttct agaataaac 
      atactcagc atggatccc tcagtggct gtgtgcttt 
      ttctgcaac actgaaggc gagttcaac gatttgtgt 
      cactcgatc aagaaagac ctcatcgag cccgaaaaa 
      cagccgcta ttcgaaatc acttatgag aaacccaaa 
      cagtggacg ccgacgctg gacgacgcc gtggaggct 
      gccgatttc gagctatcc ggagagtac gattccgag 
      cacgaattt gaaattttg taa
    mRNA sequence
    • >TC013405 RA
      ctgcgtgtg ataaacgtg attaaaaat agaaaaagc 
      caaattttt cgttcactt gacacatca gctgttgtt 
      tgacaccaa gatggcgat ctgacatta aaaatgaaa 
      acaaaccgt ggaaaacag tgattcctc aaaactcgc 
      ttacgattt gccaattta acgttaaat ccatgaata 
      tcccctttc gtgacaatg gacggcatg ttcgaacta 
      tacctagac gggagcatt gagcccgat gacatcccc 
      catactgca gaccccgtc tacatccta ggcaagaag 
      tacaacgca ctccaagaa ttggacacg atccgccaa 
      gacattctc tcgaaaatc tggttcacg tatcggaaa 
      aatttcgtt ccaattggg ggcgacgaa ggtctcacc 
      actgacaag ggctggggc tgcatgcta cgatgcggc 
      cagatggtt ctggcgcaa gccttggtc acgctgcat 
      ctgggccga gactgggtc tgggagccc gaaaccaaa 
      gactccact tatctcaaa atacttagc aaatttgta 
      gacaagaga caagcgccg ttttccata caccagatt 
      gccatgatg ggggtgtcg gaaaacaag gaagtgggg 
      cagtggttt ggacccaac actgtggcc caagtcttg 
      aagaagttg gtaaagtat gacgagtgg agtgcaatt 
      gagatgcac atagcgtta gataataca cttattatt 
      agcgatata agagagttg tgcttaagt caggggagt 
      gacggttgt agcagtggg gactggaag ccgttgcta 
      cttattgtg cctttaaga ttgggactg caagaaatt 
      aatcctatt tatgctagt ggattaaag aaatgtttt 
      cagttcaaa caaagcctg ggtgtgatc ggtggcaaa 
      ccgaacctg gcgctgtat ttcatagga catgtagga 
      gacgaagta atatatcta gacccgcac acgacgcag 
      aaatccggt tccgtggaa agcaaagaa acggaagaa 
      gaaatcgag ctggattcc acctaccac tgtaaatac 
      gcttctaga ataaacata ctcagcatg gatccctca 
      gtggctgtg tgctttttc tgcaacact gaaggcgag 
      ttcaacgat ttgtgtcac tcgatcaag aaagacctc 
      atcgagccc gaaaaacag ccgctattc gaaatcact 
      tatgagaaa cccaaacag tggacgccg acgctggac 
      gacgccgtg gaggctgcc gatttcgag ctatccgga 
      gagtacgat tccgagcac gaatttgaa attttgtaa 
      caattttct catttaact cagttttta ttgtcgcaa 
      attgtggat agaggcccg caaattgta aatattttt 
      cttactcat acttgtttc tgtacatag tgcatttac 
      cgaggggcg ccactcgaa atgttttaa cgctgccgg 
      tgccaaaaa tatcgactt attttgtac ataaatgac 
      gccgatatg tttcgtgta aagctttgt atttttcac 
      cataattgt gggtctgtg ttttgtaac gaagcgggc 
      gaacagtcg tcgcacacg acaaataat cctgcatta 
      tgttatgcc ttgacaaat aaaaagtga aatcaatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY