Status
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Gene information
General information
TC033152
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC014394,TC014395
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 10018853..10023683
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033152
    iBeetle screen iB_10065

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033152 RA
      MAKKGQAIQ LQTEVNNDE DWEKLLQRD GLIVVDVYS 
      EWCGPCSGM TATLKKLKL EIGGDVLQL AIAKADKIT 
      ALERFRNKS EPTWMFIIN GKMVNFMFG ANAPKLTRL 
      ITEELRKEN EARQGRRDR VQNEISDIT EEERERLDV 
      VERQRRAAK EKEEAKAAK ELFERRTAE CHKILETLQ 
      HMGIILIFP HAKTSYQEA FGDLLGEAG LTISQTEKV 
      DLTEQILDE LFYFGEMPF PEEALEELL NNTCMILQV 
      KPIPARDVE EPTDELILQ ILYGPSKKP PGSSDCPAR 
      VLAKLPEPS EEEVHEKTA RESVGVWAP ENELIKATA 
      LRLFFHKLT EHYLIPEPE PTPPHYAVV FEAMRRHEI 
      ASFMEKYSD QVIRYGFFT SERPEEAKL VAKSIYKYE 
      RSPDRTPNE KMVIQLAKK KSECVLAFA QLKPLYISP 
      NIKEGEREC RLFFGDDYD EPPEEEEIE EQKEVPATP 
      KSKSEQASV LDQDIEGSQ VVALEEGEI VEGVPEEGA 
      EAVAVAEEG APLVGEVPP AEGEAPPAE GVPPETEPS 
      VVVEPEASP PDQPENLVH VSEIALEEM LFFAEEEVE 
      QFQNEELFK KPSMALLMK RSSENLPNT IDDVVAQMV 
      YGNHRKPPG DENCPYRKL ITTKDNVEL PGIWAPTNS 
      LCKAMGLKW LFPNISGPY KLPEVEPTP PHVIIAYDA 
      FKYKEVMEL GNQFPSAIM KYGFFSNHT PGSAQLITK 
      TVDKFEKQT TPPTYEEKI VVQLAKSDD CFESFYDLG 
      PSYISLDTV QGAEEAQLF FPPTYDIPE DIIMEIKKK 
      PKKKTKSKF VAVVEAHEE KLLQGDIKE ENDLEEQAS 
      EDNADGRQL TEEKATEME EEEEEEGDE GGEDIVDKA 
      TSPMDDAKE EDTLNFVPT LLQMLYFTL TQLTLIFKH 
      GLADQILSV LPLWEIDSA GQETALKIR KEAKIYTFC 
      TLVTTATAV VAVLLLTSP QDNADQVII IFCKFRELF 
      PYQNWVFAR LSKLIYLLL ILAAPAHTY QTIYVTQHL 
      KFQLYLLNE YLKGLGSYK KMDRNALFY DTEYQKLVR 
      FRLTVCIKR HLEIINARN NAVGLMDNY IVPFIITGA 
      LLVAGLLLS AFHLVGEVH LPWWHCAAL GIPVFLCAC 
      YFFVSGQAL ESESERVMT TLMELDWYD WYSHNKKMF 
      LFFFK
    CDS sequence
    • >TC033152 RA
      atggccaag aagggtcaa gcaatccag ttacaaact 
      gaggtaaat aacgacgaa gactgggaa aaactctta 
      caacgtgac ggacttatt gttgttgac gtctattct 
      gaatggtgc ggcccttgc tccggaatg accgcaact 
      ttaaaaaaa ctaaaactc gagattgga ggcgacgtg 
      cttcaacta gctatagca aaagccgac aaaataacg 
      gctctagaa cgtttccgc aacaagagc gaacccact 
      tggatgttc attatcaac ggaaaaatg gttaatttc 
      atgttcgga gccaacgct cctaaattg actcgttta 
      ataactgaa gaactccgc aaagaaaac gaagcacga 
      cagggacgt cgtgacaga gtccaaaat gaaataagc 
      gacatcact gaggaagaa cgggagcgg ctggatgtt 
      gtagaacgg cagagaaga gcggcgaaa gagaaggag 
      gaggcgaaa gccgccaaa gaactgttt gagagacgc 
      acggccgag tgtcacaag attttggaa actttacag 
      cacatgggc atcatattg atttttccg cacgcgaaa 
      actagctac caggaggcg ttcggagac ctcctgggc 
      gaggctggt ttgactata agccaaaca gagaaagtg 
      gaccttacg gagcaaatt ctggacgag ttgttttat 
      tttggcgaa atgccgttt cccgaagaa gcgcttgaa 
      gaactgttg aataacact tgcatgatc ttgcaagtc 
      aaaccgatc ccagctaga gacgtcgaa gaaccgacc 
      gatgaacta atcctacaa attctgtac ggtcccagc 
      aaaaagcca ccaggaagt tcggactgt ccagctcga 
      gtcctagca aaattacct gaaccttcc gaggaggaa 
      gtccacgaa aagacagca cgtgaaagt gtgggagtt 
      tgggcgcct gaaaacgag ctgatcaaa gccacagcc 
      ttgaggctg tttttccac aagctcacc gaacattat 
      cttatacca gaaccggag cccacacct ccccactat 
      gccgtcgtg tttgaagca atgcgaagg cacgaaatt 
      gcgagcttt atggagaaa tacagcgac caggtgatt 
      cgctacggg ttctttaca agcgaaagg cccgaggag 
      gcaaaacta gtcgcgaaa tcaatttac aaatatgaa 
      agatctccc gatagaacg ccaaacgaa aaaatggtg 
      atccaactg gcgaaaaag aaaagcgag tgtgttttg 
      gctttcgca cagcttaag ccgctctac atcagtcca 
      aacatcaaa gagggtgaa agggagtgt cggttgttc 
      tttggtgat gattacgat gaacctccg gaagaagaa 
      gaaattgaa gaacaaaag gaggttcca gcgacgcca 
      aaatccaaa tcggaacaa gcgtcggtt ctggatcaa 
      gatatagaa ggaagtcag gttgtagca cttgaggaa 
      ggtgaaatt gtcgaaggc gtgccggaa gagggtgcc 
      gaagcagtt gcagtagcc gaagaaggt gctccgctt 
      gtaggagaa gttcctccc gctgaagga gaggctcct 
      ccagctgaa ggagttcca cccgagacg gaaccgtct 
      gtggttgta gaacccgag gcttcacca ccagaccaa 
      cctgaaaat ctcgtacat gttagcgaa atcgccttg 
      gaagagatg ctatttttc gccgaggaa gaagtggaa 
      caattccaa aatgaagaa ctgttcaag aaaccatcc 
      atggcgctt ttgatgaaa cgcagttct gagaatttg 
      ccaaacacc attgacgac gttgtggca caaatggtt 
      tatggcaac catcgcaaa ccaccgggt gacgaaaat 
      tgtccttat cgaaaactg ataacaacg aaagataat 
      gtcgaattg cctggaatt tgggctcct actaactcg 
      ttgtgtaag gcaatgggt ctgaaatgg ttgtttccg 
      aatatttcg ggaccttac aaacttcct gaggtggaa 
      ccgacgcca ccccacgta atcatagcc tacgatgct 
      ttcaaatac aaggaagtt atggaactg gggaaccag 
      tttccctcc gcgattatg aaatacggt tttttctca 
      aaccataca ccaggcagt gcccagttg attaccaaa 
      actgtggac aagtttgaa aaacaaaca acgcctccg 
      acttatgag gagaaaatc gttgtacaa cttgccaaa 
      tccgacgac tgctttgaa agtttttac gatttggga 
      ccgtcgtat atcagttta gacacggtt caaggggca 
      gaagaagca cagcttttt ttcccgccg acttacgat 
      attccggaa gatattatc atggaaatc aagaaaaaa 
      ccgaaaaag aagacaaag agcaaattt gttgcagtt 
      gtggaagca cacgaggag aaacttcta caaggggat 
      attaaagaa gaaaatgac ttagaggaa caagcgtcg 
      gaggataac gctgatgga cgacaactt accgaagaa 
      aaggccacg gaaatggag gaggaggag gaagaggaa 
      ggtgacgaa ggtggagag gatattgtc gataaggcg 
      acgtccccc atggacgac gccaaggag gaagacact 
      ttgaatttc gttcccaca cttttgcaa atgctttac 
      tttacgctt acacaattg acacttatt ttcaaacat 
      ggtctagcg gaccaaata ttgagcgtt ctgccattg 
      tgggaaatc gactccgcg gggcaagaa acagcgttg 
      aagataaga aaagaagca aaaatatat actttttgt 
      actttagta accacagcg actgccgtt gtggccgtg 
      ttacttttg acttctcct caggacaac gccgatcaa 
      gttatcatc attttttgc aaatttcgg gaattgttt 
      ccgtaccaa aattgggtt tttgcacgt ttatcaaag 
      cttatatat cttctgctc atattggca gctccagcg 
      cacacatat caaacgatt tatgtcaca caacacttg 
      aaatttcag ctatatctt ttgaatgaa tatctcaaa 
      ggactgggg agttataaa aaaatggat agaaatgcg 
      ttgttttat gacacagag tatcagaag ctagtcaga 
      tttagacta acagtgtgc ataaaaagg catctagaa 
      attattaat gcaagaaac aacgctgtt ggattgatg 
      gacaattac attgtgccg tttataata acaggcgcc 
      cttttggtg gccgggttg cttttatca gcatttcac 
      cttgtagga gaggtccac cttccctgg tggcattgt 
      gcagcttta ggtattcca gttttctta tgcgcttgc 
      tattttttc gttagcgga caggctctt gaatccgag 
      tccgaacgt gttatgacg acattgatg gaactagac 
      tggtacgac tggtatagc cataataaa aaaatgttt 
      ttgtttttt tttaaataa
    mRNA sequence
    • >TC033152 RA
      aacgtgtta tttaatcaa tagtagcaa acaactctt 
      agcaacttt gacaaatca agtcataat caaagttcc 
      tgacataat tagcgtaaa taatggcca agaagggtc 
      aagcaatcc agttacaaa ctgaggtaa ataacgacg 
      aagactggg aaaaactct tacaacgtg acggactta 
      ttgttgttg acgtctatt ctgaatggt gcggccctt 
      gctccggaa tgaccgcaa ctttaaaaa aactaaaac 
      tcgagattg gaggcgacg tgcttcaac tagctatag 
      caaaagccg acaaaataa cggctctag aacgtttcc 
      gcaacaaga gcgaaccca cttggatgt tcattatca 
      acggaaaaa tggttaatt tcatgttcg gagccaacg 
      ctcctaaat tgactcgtt taataactg aagaactcc 
      gcaaagaaa acgaagcac gacagggac gtcgtgaca 
      gagtccaaa atgaaataa gcgacatca ctgaggaag 
      aacgggagc ggctggatg ttgtagaac ggcagagaa 
      gagcggcga aagagaagg aggaggcga aagccgcca 
      aagaactgt ttgagagac gcacggccg agtgtcaca 
      agattttgg aaactttac agcacatgg gcatcatat 
      tgatttttc cgcacgcga aaactagct accaggagg 
      cgttcggag acctcctgg gcgaggctg gtttgacta 
      taagccaaa cagagaaag tggacctta cggagcaaa 
      ttctggacg agttgtttt attttggcg aaatgccgt 
      ttcccgaag aagcgcttg aagaactgt tgaataaca 
      cttgcatga tcttgcaag tcaaaccga tcccagcta 
      gagacgtcg aagaaccga ccgatgaac taatcctac 
      aaattctgt acggtccca gcaaaaagc caccaggaa 
      gttcggact gtccagctc gagtcctag caaaattac 
      ctgaacctt ccgaggagg aagtccacg aaaagacag 
      cacgtgaaa gtgtgggag tttgggcgc ctgaaaacg 
      agctgatca aagccacag ccttgaggc tgtttttcc 
      acaagctca ccgaacatt atcttatac cagaaccgg 
      agcccacac ctccccact atgccgtcg tgtttgaag 
      caatgcgaa ggcacgaaa ttgcgagct ttatggaga 
      aatacagcg accaggtga ttcgctacg ggttcttta 
      caagcgaaa ggcccgagg aggcaaaac tagtcgcga 
      aatcaattt acaaatatg aaagatctc ccgatagaa 
      cgccaaacg aaaaaatgg tgatccaac tggcgaaaa 
      agaaaagcg agtgtgttt tggctttcg cacagctta 
      agccgctct acatcagtc caaacatca aagagggtg 
      aaagggagt gtcggttgt tctttggtg atgattacg 
      atgaacctc cggaagaag aagaaattg aagaacaaa 
      aggaggttc cagcgacgc caaaatcca aatcggaac 
      aagcgtcgg ttctggatc aagatatag aaggaagtc 
      aggttgtag cacttgagg aaggtgaaa ttgtcgaag 
      gcgtgccgg aagagggtg ccgaagcag ttgcagtag 
      ccgaagaag gtgctccgc ttgtaggag aagttcctc 
      ccgctgaag gagaggctc ctccagctg aaggagttc 
      cacccgaga cggaaccgt ctgtggttg tagaacccg 
      aggcttcac caccagacc aacctgaaa atctcgtac 
      atgttagcg aaatcgcct tggaagaga tgctatttt 
      tcgccgagg aagaagtgg aacaattcc aaaatgaag 
      aactgttca agaaaccat ccatggcgc ttttgatga 
      aacgcagtt ctgagaatt tgccaaaca ccattgacg 
      acgttgtgg cacaaatgg tttatggca accatcgca 
      aaccaccgg gtgacgaaa attgtcctt atcgaaaac 
      tgataacaa cgaaagata atgtcgaat tgcctggaa 
      tttgggctc ctactaact cgttgtgta aggcaatgg 
      gtctgaaat ggttgtttc cgaatattt cgggacctt 
      acaaacttc ctgaggtgg aaccgacgc caccccacg 
      taatcatag cctacgatg ctttcaaat acaaggaag 
      ttatggaac tggggaacc agtttccct ccgcgatta 
      tgaaatacg gttttttct caaaccata caccaggca 
      gtgcccagt tgattacca aaactgtgg acaagtttg 
      aaaaacaaa caacgcctc cgacttatg aggagaaaa 
      tcgttgtac aacttgcca aatccgacg actgctttg 
      aaagttttt acgatttgg gaccgtcgt atatcagtt 
      tagacacgg ttcaagggg cagaagaag cacagcttt 
      ttttcccgc cgacttacg atattccgg aagatatta 
      tcatggaaa tcaagaaaa aaccgaaaa agaagacaa 
      agagcaaat ttgttgcag ttgtggaag cacacgagg 
      agaaacttc tacaagggg atattaaag aagaaaatg 
      acttagagg aacaagcgt cggaggata acgctgatg 
      gacgacaac ttaccgaag aaaaggcca cggaaatgg 
      aggaggagg aggaagagg aaggtgacg aaggtggag 
      aggatattg tcgataagg cgacgtccc ccatggacg 
      acgccaagg aggaagaca ctttgaatt tcgttccca 
      cacttttgc aaatgcttt actttacgc ttacacaat 
      tgacactta ttttcaaac atggtctag cggaccaaa 
      tattgagcg ttctgccat tgtgggaaa tcgactccg 
      cggggcaag aaacagcgt tgaagataa gaaaagaag 
      caaaaatat atacttttt gtactttag taaccacag 
      cgactgccg ttgtggccg tgttacttt tgacttctc 
      ctcaggaca acgccgatc aagttatca tcatttttt 
      gcaaatttc gggaattgt ttccgtacc aaaattggg 
      tttttgcac gtttatcaa agcttatat atcttctgc 
      tcatattgg cagctccag cgcacacat atcaaacga 
      tttatgtca cacaacact tgaaatttc agctatatc 
      ttttgaatg aatatctca aaggactgg ggagttata 
      aaaaaatgg atagaaatg cgttgtttt atgacacag 
      agtatcaga agctagtca gatttagac taacagtgt 
      gcataaaaa ggcatctag aaattatta atgcaagaa 
      acaacgctg ttggattga tggacaatt acattgtgc 
      cgtttataa taacaggcg cccttttgg tggccgggt 
      tgcttttat cagcatttc accttgtag gagaggtcc 
      accttccct ggtggcatt gtgcagctt taggtattc 
      cagttttct tatgcgctt gctattttt tcgttagcg 
      gacaggctc ttgaatccg agtccgaac gtgttatga 
      cgacattga tggaactag actggtacg actggtata 
      gccataata aaaaaatgt ttttgtttt tttttaaat 
      aacacttta aaaccacta caaatttcg tttgtaaat 
      gtcgccctc aattatgtg acaggagtt aaagtatga 
      aacgttgtt tagctattt tttctacag agttttgta 
      ggttctgaa attggttta cactgcagt ttcaatttt 
      cggcagttt caaataaat gtttcgcag gataagaaa 
      agtgcacta aacacttta cgtgcactc gtataatgt 
      cttgtattc tacgtgtcg ttaataaat atttaccaa 
      gcac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY