Status
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Gene information
General information
TC013419 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9902770..9904151
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013419
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10060

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013419 RA
      MSTCGVVFL MIIIQRGKY QEFVNKYVV 
      RPMKSLLPH KETSEASDE KDKESGNFD 
      FIGRDIIRE TSKVAKTIG KNIKEFVSK 
      NTKGLDCDK EFSGEMVDG FKSKVKAIY 
      PGTKWCGDG NISKSYDDL GKFADTDKC 
      CREHDMCPI NIDAGATKY DLVNTGLFT 
      RSHCDCDKK FYDCLKEAG GVVAESIGF 
      TYFTVLGPQ CFKEEYPII GCLDTKEKK 
      CLQYVTDTR RPKMYQWFD SPTF
    • >TC013419 RB
      MSTCGVVFL MIIIQRGKY QDHSLPFGF NLRSKVQVE 
      LNHQQLPVN AMWSKSVVI LLFAVCFSA FFVENNGLE 
      VKEFVNKYV VRPMKSLLP HKETSEASD EKDKESGNF 
      DFIGRDIIR ETSKVAKTI GKNIKEFVS KNTKGLDCD 
      KEFSGEMVD GFKSKVKAI YPGTKWCGD GNISKSYDD 
      LGKFADTDK CCREHDMCP INIDAGATK YDLVNTGLF 
      TRSHCDCDK KFYDCLKEA GGVVAESIG FTYFTVLGP 
      QCFKEEYPI IGCLDTKEK KCLQYVTDT RRPKMYQWF 
      DSPTF
    • >TC013419 RC
      MWSKSVVIL LFAVCFSAF FVENNGLEV KEFVNKYVV 
      RPMKSLLPH KETSEASDE KDKESGNFD FIGRDIIRE 
      TSKVAKTIG KNIKEFVSK NTKGLDCDK EFSGEMVDG 
      FKSKVKAIY PGTKWCGDG NISKSYDDL GKFADTDKC 
      CREHDMCPI NIDAGATKY DLVNTGLFT RSHCDCDKK 
      FYDCLKEAG GVVAESIGF TYFTVLGPQ CFKEEYPII 
      GCLDTKEKK CLQYVTDTR RPKMYQWFD SPTF
    CDS sequence
    • >TC013419 RA
      atgtccaca tgtggtgta gtatttctc atgattatt 
      atccaacgg gggaagtat caagaattc gttaacaag 
      tatgtggtg cgtcctatg aaaagtttg cttccacac 
      aaagaaact tctgaggct agtgatgag aaagataaa 
      gaaagtggc aatttcgat tttattggg cgcgatata 
      atcagagag acgtcaaaa gtggccaaa acaatcggg 
      aaaaacatc aaggaattt gtgtcgaaa aataccaaa 
      gggctggac tgtgataag gaattttcg ggtgaaatg 
      gtcgacggt ttcaagtcg aaagttaag gcaatttat 
      cccggtacc aaatggtgc ggcgatggt aacatttct 
      aaatcttac gacgatttg ggcaagttt gccgatact 
      gataagtgc tgcagagag cacgatatg tgtcccatt 
      aacatagat gcaggtgca acaaaatac gacttggta 
      aatactggg ctatttaca agatcgcac tgtgactgt 
      gataagaaa ttttacgat tgccttaaa gaggcaggc 
      ggtgttgtt gctgaaagc ataggattc acttacttt 
      actgttctt ggacctcag tgtttcaag gaagaatat 
      ccaattatt ggatgtctg gatacgaaa gagaaaaag 
      tgcctccag tatgtaaca gatacacgg agaccgaag 
      atgtaccag tggttcgac agtcctact ttttaa
    • >TC013419 RB
      atgtccaca tgtggtgta gtatttctc atgattatt 
      atccaacgg gggaagtat caagaccac tcattacca 
      tttggtttt aatttgcgg tcgaaggtg caagttgaa 
      cttaatcat cagcaatta ccagtgaac gcaatgtgg 
      tccaaatca gtcgtaatt ttattattc gcagtttgc 
      tttagcgcg ttttttgtt gaaaataac ggattggaa 
      gtgaaagaa ttcgttaac aagtatgtg gtgcgtcct 
      atgaaaagt ttgcttcca cacaaagaa acttctgag 
      gctagtgat gagaaagat aaagaaagt ggcaatttc 
      gattttatt gggcgcgat ataatcaga gagacgtca 
      aaagtggcc aaaacaatc gggaaaaac atcaaggaa 
      tttgtgtcg aaaaatacc aaagggctg gactgtgat 
      aaggaattt tcgggtgaa atggtcgac ggtttcaag 
      tcgaaagtt aaggcaatt tatcccggt accaaatgg 
      tgcggcgat ggtaacatt tctaaatct tacgacgat 
      ttgggcaag tttgccgat actgataag tgctgcaga 
      gagcacgat atgtgtccc attaacata gatgcaggt 
      gcaacaaaa tacgacttg gtaaatact gggctattt 
      acaagatcg cactgtgac tgtgataag aaattttac 
      gattgcctt aaagaggca ggcggtgtt gttgctgaa 
      agcatagga ttcacttac tttactgtt cttggacct 
      cagtgtttc aaggaagaa tatccaatt attggatgt 
      ctggatacg aaagagaaa aagtgcctc cagtatgta 
      acagataca cggagaccg aagatgtac cagtggttc 
      gacagtcct actttttaa
    • >TC013419 RC
      atgtggtcc aaatcagtc gtaatttta ttattcgca 
      gtttgcttt agcgcgttt tttgttgaa aataacgga 
      ttggaagtg aaagaattc gttaacaag tatgtggtg 
      cgtcctatg aaaagtttg cttccacac aaagaaact 
      tctgaggct agtgatgag aaagataaa gaaagtggc 
      aatttcgat tttattggg cgcgatata atcagagag 
      acgtcaaaa gtggccaaa acaatcggg aaaaacatc 
      aaggaattt gtgtcgaaa aataccaaa gggctggac 
      tgtgataag gaattttcg ggtgaaatg gtcgacggt 
      ttcaagtcg aaagttaag gcaatttat cccggtacc 
      aaatggtgc ggcgatggt aacatttct aaatcttac 
      gacgatttg ggcaagttt gccgatact gataagtgc 
      tgcagagag cacgatatg tgtcccatt aacatagat 
      gcaggtgca acaaaatac gacttggta aatactggg 
      ctatttaca agatcgcac tgtgactgt gataagaaa 
      ttttacgat tgccttaaa gaggcaggc ggtgttgtt 
      gctgaaagc ataggattc acttacttt actgttctt 
      ggacctcag tgtttcaag gaagaatat ccaattatt 
      ggatgtctg gatacgaaa gagaaaaag tgcctccag 
      tatgtaaca gatacacgg agaccgaag atgtaccag 
      tggttcgac agtcctact ttttaa
    mRNA sequence
    • >TC013419 RA
      ctgggaact cccgaggta actaacatc ctgcctgaa 
      tcacagtag ccgctaaat atgcaaatg cggttacca 
      acgagatta tccgaaaac ccacctaaa atcaaaatt 
      cgggcaaaa cctcgggaa tatctcgca tctatgtaa 
      ttcccacaa cgaacttta ataatggtg gagtagcaa 
      tcatcaatc aaacgcgta ataacccat cggtcttga 
      acaacttct acgtcaaag aaatcgtaa tgtccacat 
      gtggtgtag tatttctca tgattatta tccaacggg 
      ggaagtatc aagaattcg ttaacaagt atgtggtgc 
      gtcctatga aaagtttgc ttccacaca aagaaactt 
      ctgaggcta gtgatgaga aagataaag aaagtggca 
      atttcgatt ttattgggc gcgatataa tcagagaga 
      cgtcaaaag tggccaaaa caatcggga aaaacatca 
      aggaatttg tgtcgaaaa ataccaaag ggctggact 
      gtgataagg aattttcgg gtgaaatgg tcgacggtt 
      tcaagtcga aagttaagg caatttatc ccggtacca 
      aatggtgcg gcgatggta acatttcta aatcttacg 
      acgatttgg gcaagtttg ccgatactg ataagtgct 
      gcagagagc acgatatgt gtcccatta acatagatg 
      caggtgcaa caaaatacg acttggtaa atactgggc 
      tatttacaa gatcgcact gtgactgtg ataagaaat 
      tttacgatt gccttaaag aggcaggcg gtgttgttg 
      ctgaaagca taggattca cttacttta ctgttcttg 
      gacctcagt gtttcaagg aagaatatc caattattg 
      gatgtctgg atacgaaag agaaaaagt gcctccagt 
      atgtaacag atacacgga gaccgaaga tgtaccagt 
      ggttcgaca gtcctactt tttaatttc ctgaatcag 
      cgttgaaat gtataataa tttatttaa ataaagatt 
      ttttatttt t
    • >TC013419 RB
      ctgggaact cccgaggta actaacatc ctgcctgaa 
      tcacagtag ccgctaaat atgcaaatg cggttacca 
      acgagatta tccgaaaac ccacctaaa atcaaaatt 
      cgggcaaaa cctcgggaa tatctcgca tctatgtaa 
      ttcccacaa cgaacttta ataatggtg gagtagcaa 
      tcatcaatc aaacgcgta ataacccat cggtcttga 
      acaacttct acgtcaaag aaatcgtaa tgtccacat 
      gtggtgtag tatttctca tgattatta tccaacggg 
      ggaagtatc aagaccact cattaccat ttggtttta 
      atttgcggt cgaaggtgc aagttgaac ttaatcatc 
      agcaattac cagtgaacg caatgtggt ccaaatcag 
      tcgtaattt tattattcg cagtttgct ttagcgcgt 
      tttttgttg aaaataacg gattggaag tgaaagaat 
      tcgttaaca agtatgtgg tgcgtccta tgaaaagtt 
      tgcttccac acaaagaaa cttctgagg ctagtgatg 
      agaaagata aagaaagtg gcaatttcg attttattg 
      ggcgcgata taatcagag agacgtcaa aagtggcca 
      aaacaatcg ggaaaaaca tcaaggaat ttgtgtcga 
      aaaatacca aagggctgg actgtgata aggaatttt 
      cgggtgaaa tggtcgacg gtttcaagt cgaaagtta 
      aggcaattt atcccggta ccaaatggt gcggcgatg 
      gtaacattt ctaaatctt acgacgatt tgggcaagt 
      ttgccgata ctgataagt gctgcagag agcacgata 
      tgtgtccca ttaacatag atgcaggtg caacaaaat 
      acgacttgg taaatactg ggctattta caagatcgc 
      actgtgact gtgataaga aattttacg attgcctta 
      aagaggcag gcggtgttg ttgctgaaa gcataggat 
      tcacttact ttactgttc ttggacctc agtgtttca 
      aggaagaat atccaatta ttggatgtc tggatacga 
      aagagaaaa agtgcctcc agtatgtaa cagatacac 
      ggagaccga agatgtacc agtggttcg acagtccta 
      ctttttaat ttcctgaat cagcgttga aatgtataa 
      taatttatt taaataaag
    • >TC013419 RC
      ctgggaact cccgagacc actcattac catttggtt 
      ttaatttgc ggtcgaagg tgcaagttg aacttaatc 
      atcagcaat taccagtga acgcaatgt ggtccaaat 
      cagtcgtaa ttttattat tcgcagttt gctttagcg 
      cgttttttg ttgaaaata acggattgg aagtgaaag 
      aattcgtta acaagtatg tggtgcgtc ctatgaaaa 
      gtttgcttc cacacaaag aaacttctg aggctagtg 
      atgagaaag ataaagaaa gtggcaatt tcgatttta 
      ttgggcgcg atataatca gagagacgt caaaagtgg 
      ccaaaacaa tcgggaaaa acatcaagg aatttgtgt 
      cgaaaaata ccaaagggc tggactgtg ataaggaat 
      tttcgggtg aaatggtcg acggtttca agtcgaaag 
      ttaaggcaa tttatcccg gtaccaaat ggtgcggcg 
      atggtaaca tttctaaat cttacgacg atttgggca 
      agtttgccg atactgata agtgctgca gagagcacg 
      atatgtgtc ccattaaca tagatgcag gtgcaacaa 
      aatacgact tggtaaata ctgggctat ttacaagat 
      cgcactgtg actgtgata agaaatttt acgattgcc 
      ttaaagagg caggcggtg ttgttgctg aaagcatag 
      gattcactt actttactg ttcttggac ctcagtgtt 
      tcaaggaag aatatccaa ttattggat gtctggata 
      cgaaagaga aaaagtgcc tccagtatg taacagata 
      cacggagac cgaagatgt accagtggt tcgacagtc 
      ctacttttt aatttcctg aatcagcgt tgaaatgta 
      taataattt atttaaata aagattttt tattttt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY