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Gene information
General information
TC014374 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9894073..9897271
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014374
    iBeetle screen iB_10059

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature fluorescence decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014374 RA
      MLLRAISKF STEEQTNYV TRLEQDFEV GDSLGEGGF 
      GQIIKARRK CDDNEFAIK IIKFKSNLK DIETTRREA 
      NVLSELNHN NIVRYYNSW TVTANMPLD EYKKLCSDS 
      EESLSTEVY SLSTKGYIS EDNGVEEEE NSESDEDSF 
      VVFQPEPEY KKVKYLFIQ MELCENNTL RHAIDNDLY 
      KDEIKLSKY FREICEGLS YIHKRNIVH RDLKPENIF 
      LTSEDVIKI GDFGLSKKI LPDNNLESV QGNELPCGS 
      LTDVCGTPI YIAPELRYR TSCSIKSDI YSLGVIYFE 
      MCSKSMTKM EKSLVFPIT RKYLQGLNF LTSNSKKKF 
      LIRILLHLN DTERPTCDE ILKILNSEK SIEEMKEMI 
      QQNRKRPYQ HYKNFEKTL KSVCAKKDE IVLFISQAI 
      QIFELHGGQ NITLPQFIP PLYNASDNL INLLNTRGN 
      SVSLASTST IAFSHYVAS NNIKKMRRF SVGKVFEEG 
      VFSPNEKQE CVFQVISPV KNDHVVEAE MLYIANEIF 
      GKNLQRSGS LYYIINHRL IFSTILKHC KMTNRKAAD 
      FINHMKNAM YTGGGLMIN ADFELEPEV RKFCEKLSG 
      FFSIKEAED CLLMTDNNG KEAFEKIYT NVLSTAFKL 
      LRIVIENAR KFGITSNIV VCPLLINER HSTGMMFKI 
      MYISRIIGS HPPNVRTEE PSIVAMGGG FGSLIQRCN 
      KMKKKKKNF VCALSAVEI SFNIETILK FLKYNCTTR 
      VIDVLIVAQ EKSNAVSLF KLLLSKGIK SEIVNELVF 
      YDTSNERPL FVVELFGEN DGVILVYSV AEDTSIPWS 
      LTEVFRGNL TTRKLLKIC DSSGN
    CDS sequence
    • >TC014374 RA
      atgttgctc cgagcgatt tcaaaattt agcactgag 
      gagcaaacc aattatgta acgagattg gaacaagat 
      ttcgaagtc ggggattca ctgggtgaa ggcggtttt 
      ggacagatt atcaaggcg agacggaaa tgtgatgat 
      aacgaattt gcgataaaa ataatcaaa tttaaatcc 
      aatttgaag gacatagaa actaccaga agagaagcg 
      aatgtattg tctgaactt aatcacaac aatattgtg 
      aggtactac aattcttgg actgtgaca gcaaacatg 
      ccgttagat gaatataag aagctatgt tctgatagt 
      gaagaatct ttaagcaca gaggtttac agtttatcc 
      acaaagggc tacattagt gaagataat ggagtcgaa 
      gaagaggaa aactcggaa agtgacgaa gacagcttt 
      gttgttttc cagccagaa cctgaatac aaaaaagta 
      aaatacctc tttattcaa atggaattg tgcgaaaat 
      aatacttta agacatgcc attgacaat gatctttat 
      aaagatgaa atcaaatta agtaaatat tttcgtgaa 
      atttgcgaa ggtttgtca tacattcac aaaagaaac 
      atcgttcat cgagattta aaaccagaa aatattttt 
      ttaacttca gaagacgtt atcaaaatt ggagacttt 
      ggattgtca aaaaagatt ttacctgat aataatctt 
      gaatcagta caaggtaat gaattgcca tgtggatct 
      ttaactgac gtttgtggt actcctatc tacatagct 
      ccagagcta cgttataga acatcatgt agtatcaag 
      agtgatatt tacagttta ggagtaata tattttgaa 
      atgtgttcc aaatctatg acgaaaatg gaaaagtcg 
      ttagtgttt ccgattaca agaaaatac ctacagggg 
      cttaatttt ttgaccagt aactctaaa aagaagttt 
      cttataaga attctttta catttaaac gatactgaa 
      aggcctacc tgcgatgaa atattgaaa atattgaat 
      tcagaaaag tctattgaa gaaatgaaa gaaatgatt 
      caacaaaac agaaaaaga ccttatcaa cactataaa 
      aacttcgaa aagacatta aagagtgtt tgtgctaag 
      aaagacgaa atagtatta tttataagt caagctatt 
      cagattttt gagttacat ggagggcaa aatattact 
      ttgcctcag ttcatacca ccattatac aacgcttct 
      gataactta atcaattta cttaataca cgaggaaat 
      tctgtttct ctagccagc acttctaca attgcattt 
      tctcattat gtggcatcg aataacatc aaaaaaatg 
      cgtcgcttt tctgtgggc aaggtgttt gaggaaggt 
      gtcttttcg ccaaatgaa aaacaagaa tgcgttttt 
      caagtaatc agtcctgta aaaaatgat catgtggta 
      gaagccgaa atgttgtat attgcgaat gaaatattc 
      ggtaaaaat ctacaacgt tccggttct ctttattac 
      atcattaat caccgactt atcttttcg acaatttta 
      aagcactgc aaaatgaca aacagaaaa gctgctgat 
      tttattaac cacatgaaa aacgctatg tatacaggt 
      ggaggtctt atgattaac gcagatttt gagctggaa 
      cctgaagtt cgtaagttt tgtgaaaaa ctttcaggc 
      tttttttca attaaagaa gccgaagac tgtttatta 
      atgacggac aacaatggt aaagaagca tttgaaaaa 
      atttacaca aatgtactt tcaactgct ttcaagctg 
      ctgcgaatt gtcattgaa aatgcaagg aaatttgga 
      attacttct aatatcgtt gtttgtcca cttcttata 
      aatgaaaga cactcaacg ggaatgatg tttaaaatt 
      atgtacata tcccgaatt attggtagc catccacca 
      aatgttaga acagaagaa ccatcaatt gttgcaatg 
      ggtggagga tttggctcg ttaattcaa agatgtaat 
      aaaatgaaa aagaaaaag aaaaatttt gtctgtgca 
      ctctctgct gttgagatt agttttaac atcgaaact 
      attttaaaa ttcttaaaa tacaattgc accacacga 
      gttattgac gtcttaatt gtggctcag gaaaaatct 
      aatgcagtt tcattgttt aaattgcta ttgtcaaaa 
      ggaataaag tctgaaatt gtgaatgaa ttagttttc 
      tatgataca tcaaacgag cgaccattg tttgttgtt 
      gaacttttt ggtgaaaat gatggagtc atactcgta 
      tatagcgta gcagaagac acaagcata ccgtggtct 
      ttaactgag gtttttaga ggtaatctc accacgagg 
      aaactttta aaaatttgt gacagttcc ggtaattaa
    mRNA sequence
    • >TC014374 RA
      aagaatttg cattacaat ttgataata tttgcgctc 
      tgtagtaga gcaaataag ttgtgtgaa atgtgacca 
      agaatgttg ctccgagcg atttcaaaa tttagcact 
      gaggagcaa accaattat gtaacgaga ttggaacaa 
      gatttcgaa gtcggggat tcactgggt gaaggcggt 
      tttggacag attatcaag gcgagacgg aaatgtgat 
      gataacgaa tttgcgata aaaataatc aaatttaaa 
      tccaatttg aaggacata gaaactacc agaagagaa 
      gcgaatgta ttgtctgaa cttaatcac aacaatatt 
      gtgaggtac tacaattct tggactgtg acagcaaac 
      atgccgtta gatgaatat aagaagcta tgttctgat 
      agtgaagaa tctttaagc acagaggtt tacagttta 
      tccacaaag ggctacatt agtgaagat aatggagtc 
      gaagaagag gaaaactcg gaaagtgac gaagacagc 
      tttgttgtt ttccagcca gaacctgaa tacaaaaaa 
      gtaaaatac ctctttatt caaatggaa ttgtgcgaa 
      aataatact ttaagacat gccattgac aatgatctt 
      tataaagat gaaatcaaa ttaagtaaa tattttcgt 
      gaaatttgc gaaggtttg tcatacatt cacaaaaga 
      aacatcgtt catcgagat ttaaaacca gaaaatatt 
      tttttaact tcagaagac gttatcaaa attggagac 
      tttggattg tcaaaaaag attttacct gataataat 
      cttgaatca gtacaaggt aatgaattg ccatgtgga 
      tctttaact gacgtttgt ggtactcct atctacata 
      gctccagag ctacgttat agaacatca tgtagtatc 
      aagagtgat atttacagt ttaggagta atatatttt 
      gaaatgtgt tccaaatct atgacgaaa atggaaaag 
      tcgttagtg tttccgatt acaagaaaa tacctacag 
      gggcttaat tttttgacc agtaactct aaaaagaag 
      tttcttata agaattctt ttacattta aacgatact 
      gaaaggcct acctgcgat gaaatattg aaaatattg 
      aattcagaa aagtctatt gaagaaatg aaagaaatg 
      attcaacaa aacagaaaa agaccttat caacactat 
      aaaaacttc gaaaagaca ttaaagagt gtttgtgct 
      aagaaagac gaaatagta ttatttata agtcaagct 
      attcagatt tttgagtta catggaggg caaaatatt 
      actttgcct cagttcata ccaccatta tacaacgct 
      tctgataac ttaatcaat ttacttaat acacgagga 
      aattctgtt tctctagcc agcacttct acaattgca 
      ttttctcat tatgtggca tcgaataac atcaaaaaa 
      atgcgtcgc ttttctgtg ggcaaggtg tttgaggaa 
      ggtgtcttt tcgccaaat gaaaaacaa gaatgcgtt 
      tttcaagta atcagtcct gtaaaaaat gatcatgtg 
      gtagaagcc gaaatgttg tatattgcg aatgaaata 
      ttcggtaaa aatctacaa cgttccggt tctctttat 
      tacatcatt aatcaccga cttatcttt tcgacaatt 
      ttaaagcac tgcaaaatg acaaacaga aaagctgct 
      gattttatt aaccacatg aaaaacgct atgtataca 
      ggtggaggt cttatgatt aacgcagat tttgagctg 
      gaacctgaa gttcgtaag ttttgtgaa aaactttca 
      ggctttttt tcaattaaa gaagccgaa gactgttta 
      ttaatgacg gacaacaat ggtaaagaa gcatttgaa 
      aaaatttac acaaatgta ctttcaact gctttcaag 
      ctgctgcga attgtcatt gaaaatgca aggaaattt 
      ggaattact tctaatatc gttgtttgt ccacttctt 
      ataaatgaa agacactca acgggaatg atgtttaaa 
      attatgtac atatcccga attattggt agccatcca 
      ccaaatgtt agaacagaa gaaccatca attgttgca 
      atgggtgga ggatttggc tcgttaatt caaagatgt 
      aataaaatg aaaaagaaa aagaaaaat tttgtctgt 
      gcactctct gctgttgag attagtttt aacatcgaa 
      actatttta aaattctta aaatacaat tgcaccaca 
      cgagttatt gacgtctta attgtggct caggaaaaa 
      tctaatgca gtttcattg tttaaattg ctattgtca 
      aaaggaata aagtctgaa attgtgaat gaattagtt 
      ttctatgat acatcaaac gagcgacca ttgtttgtt 
      gttgaactt tttggtgaa aatgatgga gtcatactc 
      gtatatagc gtagcagaa gacacaagc ataccgtgg 
      tctttaact gaggttttt agaggtaat ctcaccacg 
      aggaaactt ttaaaaatt tgtgacagt tccggtaat 
      taaaacttg acatgttag atatacgtt acgagagtt 
      aacattcta catacttgt tatatctat tttggttta 
      ttgtggtta aaacagatg tcttaaata aaagtatta 
      ataaata
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY