Status
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Gene information
General information
TC014372 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9886233..9888994
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014372
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_08316

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    larva segment random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_10058

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014372 RA
      MGNAELDII DGADYVNHY KNLKILHCN 
      NQIKELQTI LRDRETSRS DFKFSADRL 
      IRLVIEESL NQLPFTDCK VKTPTNSIY 
      DGLKYKSGN CGVSIVRSG EAMEQGLRD 
      CCRSIRIGK ILVESDADT HEAKVVYAR 
      FPEDIAQRH VLLMYPIMS TGNTVSKAV 
      TVLKEHKVK EENIILSNL FCTPAAVQT 
      VLESFPKLK MLTSEVHPV APNHFGQKY 
      FGTD
    CDS sequence
    • >TC014372 RA
      atgggaaat gccgaattg gatataatt gatggggcc 
      gattatgtt aatcattac aaaaactta aagatcttg 
      cattgcaac aatcaaatt aaagagctt caaacaatt 
      ttacgagac agggagact tcaaggagc gattttaag 
      ttttctgct gatagactt atccgctta gttattgag 
      gaaagtcta aaccagttg ccgttcact gactgtaaa 
      gtgaaaact ccaacaaat tcgatttat gatggcttg 
      aaatacaag tcggggaat tgtggggtg tctattgta 
      cgctctggg gaagctatg gagcagggg ttgcgagac 
      tgttgtcgt agtattagg ataggaaaa attttggtt 
      gaatctgat gctgacact cacgaggct aaagtggtc 
      tatgccagg tttccagaa gacattgcc caaagacac 
      gttttgctg atgtatcca atcatgtca acggggaat 
      accgtcagc aaagctgtt acagtttta aaggagcac 
      aaagtcaaa gaagaaaat attatttta agtaattta 
      ttttgcaca cctgctgct gtacaaacg gtcttagaa 
      tcgtttcca aaattaaaa atgttgact tcggaggtt 
      catccagtg gccccgaat cattttgga caaaagtat 
      tttggtact gattga
    mRNA sequence
    • >TC014372 RA
      tgatccgca caaccaccc ttgatttga ttgaaaaca 
      tgccccgtt gattgtcga ggggttgat tatattaaa 
      ttcttctga tcggcttgt ttctagtgg ttttgttat 
      ggaaatcac gttattttt gtttcagga ttacggagc 
      gggagggtg gagagtctc aatttttca atttattag 
      atgcactat tatacactt attgtttta tttttattg 
      tataaatat tattgtaca tgttggaaa tcaataaat 
      ctattgttt atttactat atcacagag tttttctat 
      cagcgattt tcgataccc caaaaaatc aacaatatg 
      agggtccaa gtccgaatt ttgcaaaat tgtctcgac 
      ttgccttaa ctggaaccg tagcaattt caggcacaa 
      ctggaccaa atcacatgt aaattcttc ggggtaatc 
      gtcattata aataaccga agttaaatt tttcgtgcc 
      aatagtacc aaaagcaac gaagagaaa tgctttcaa 
      attaaatga tgcccctgc ttcggtcag gtacaaaca 
      cttgtctaa gttatgact acggataaa cttatactg 
      aaaatcgtt caaattcgg ttccaaaac cctgaaaac 
      ttcctcact ttgaatcgg tatggaatc gttcgagtt 
      cccgggaat aaaaaaccc gtactctat caaaagctt 
      gacgttatc gtggtcccc gtaagaaat aaagacttg 
      ttcgtgttt cctcgtccc gactaaagt agttaactg 
      gtagacaaa cttcccttg gtcattcat caacgtggc 
      ttcggtctt cgcctcatg gctgtgatt aaacatatc 
      cagaaaggg gcacaaggc catgcaagg ctcatctga 
      tagaatatc gctcccgca atcgcataa ttcgcgaac 
      aattcgagg attgacgta aacagccct agtattgac 
      caaaacgta cccccaaat aaacgaatg caagtcaat 
      cacgcaact tgcctccct ccttattga ttttcttag 
      cctcgacca gctttcctc gaggagtct tcgtgactt 
      cttcgcggc taatcttaa atcagccgg aagtaattc 
      aacacgatc ctgtgacaa aagtgcggg aatccaagg 
      tggttgcgg gggcaagga ttttatgta gtccctcca 
      aaacgaatt ttctttgtc cctttcgat cactagaaa 
      tgcggtcaa taagtcgac gattgatag tcgactttc 
      gggggtgaa caaatgcaa gaaattcga tttttggag 
      ggtggtgta gcttatcat taattcgta cggcactgt 
      gattaaaac atcgctttc tttgagatt cctgggggt 
      tgctacccc tcgaccggt gtcgggaaa attgcgcag 
      ttttaagtt aattgggtc gttaaaacc gttgcgtgc 
      catgaattt tctcaagtt aattgcgtt ttcttcgtg 
      atccaacgg ttgaggacg gtggtgttt gccgcgttt 
      gcgcccagt acggcccat tatgctacg ccactggca 
      actggcaag tgacgtttc gcaataacc ttactttta 
      agtaactgc acacatttt gtgatattt aaggctaat 
      aattaatca tcacacaac tatcgacac ttgtggagc 
      accagaact gtcacactt ttttaacct cactttcta 
      taacaaaaa acatgggaa atgccgaat tggatataa 
      ttgatgggg ccgattatg ttaatcatt acaaaaact 
      taaagatct tgcattgca acaatcaaa ttaaagagc 
      ttcaaacaa ttttacgag acagggaga cttcaagga 
      gcgatttta agttttctg ctgatagac ttatccgct 
      tagttattg aggaaagtc taaaccagt tgccgttca 
      ctgactgta aagtgaaaa ctccaacaa attcgattt 
      atgatggct tgaaataca agtcgggga attgtgggg 
      tgtctattg tacgctctg gggaagcta tggagcagg 
      ggttgcgag actgttgtc gtagtatta ggataggaa 
      aaattttgg ttgaatctg atgctgaca ctcacgagg 
      ctaaagtgg tctatgcca ggtttccag aagacattg 
      cccaaagac acgttttgc tgatgtatc caatcatgt 
      caacgggga ataccgtca gcaaagctg ttacagttt 
      taaaggagc acaaagtca aagaagaaa atattattt 
      taagtaatt tattttgca cacctgctg ctgtacaaa 
      cggtcttag aatcgtttc caaaattaa aaatgttga 
      cttcggagg ttcatccag tggccccga atcattttg 
      gacaaaagt attttggta ctgattgaa aggacttct 
      ttcaaagtt tgtgattta tattattag catttaata 
      tttgttaga tcaataaaa tttgcacga gtgcatatt 
      acttaattt tgaaaggtt tgaaatttt tatttttgg 
      atacgttgt gccaaatgt gtgcgaata tttaatccc 
      ttttgtgta atttatttg tcttgtgtt gtagttttc 
      tgttaataa agcagtatg aaaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY