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Gene information
General information
TC031147 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9407211..9409913
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031147
      iBeetle screen iB_10056

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031147 RA
        MYTTTRRLS AHIHLLDAA STDQRGEEA 
        TLSLALHDH FELCVSVLC TFVTEKDDK 
        RNFLRLDGN FLHCNSISS LPTPRFSLS 
        NSLHAASPY SSSPKRTRK LRAS
      CDS sequence
      • >TC031147 RA
        atgtacact accactcgc cgcctctct gctcatatc 
        catctactc gacgccgcc tccaccgat caacgcggc 
        gaggaggcg acactttct ctcgcgctc cacgaccac 
        tttgagctg tgtgtatcg gttttgtgc accttcgtc 
        actgagaaa gacgacaaa cgcaatttc cttcgcctg 
        gatggcaat ttccttcat tgcaattca atttcgtcg 
        cttccaact ccacgcttc tccttatca aattcacta 
        cacgccgct tctccttac tcctcttca cctaagcga 
        actagaaag ctaagagca agctaa
      mRNA sequence
      • >TC031147 RA
        tagtacgat gcaagcaac aaaattaga actctcatt 
        tatttttta agttcgctg gctatagta gacctgacc 
        tgacttgac ttatcaaag atatattca atacagtgg 
        tttgtttcc tagttcgtt aaacagggt tatgggttg 
        tttttgggt gtgtatatc tagtcttaa gctatctta 
        ggctagtct taggtggta gggacctta aaagtaaga 
        tacgtgtgg tactgttgg ctggagcgt caccagggc 
        ccccgcccg cattggggg ccttggcgt cgctccgct 
        ggccttatc cacttacct cctcggcgg cccgtgatc 
        gggagggag ggggatcgg aactacaac tgtcgcgtc 
        atcatcgca agttcgcaa gagccaatt aaatggccg 
        acacggtct agagacaac gtacacaca ttggagaac 
        cgacagctg agagtgact tacagaact gatataact 
        gatagtgtg tgcggcaaa agaacgtca aaacaacac 
        gtgtgaagc ggggaggtg ctaccggtt agtcactaa 
        agccagcaa gccagccac acgggggtt ttgaccttg 
        ttttaccgg ggagggacg ggacaggcc agtgctaca 
        cgtagagct atatcatgc acacctcta tttttcttc 
        cctcaacca cggtgccgt gtagtcggt ctcgtgcgc 
        gctccgcat cagcactat ttgcgaatg ttacaaaac 
        cgaacccct gcatgcaga gacagacaa gacatgcat 
        tataaacag ctcttcaaa ctcgtgcaa tgacatgat 
        gacgttaga cattagaca tgcttctgt ctagaagcc 
        tcattccac actcgctca ctagtctcg gctctcggg 
        ttagactac tttacactg ttaagcctc aagtcggcg 
        gcggcattc gctccacgt gacgggagt gcgccagtg 
        cgcctagct atgtacact accactcgc cgcctctct 
        gctcatatc catctactc gacgccgcc tccaccgat 
        caacgcggc gaggaggcg acactttct ctcgcgctc 
        cacgaccac tttgagctg tgtgtatcg gttttgtgc 
        accttcgtc actgagaaa gacgacaaa cgcaatttc 
        cttcgcctg gatggcaat ttccttcat tgcaattca 
        atttcgtcg cttccaact ccacgcttc tccttatca 
        aattcacta cacgccgct tctccttac tcctcttca 
        cctaagcga actagaaag ctaagagca agctaaagg 
        gaaccttgc cactccaca ctattatct ctgttaata 
        cacataaaa ctacaacaa aattgactg tattttctt 
        tgccggtgt gggtgtttg tgcaggcga aatacacgg 
        atgtgagtg ttgttgccg acctccgct ctagctcgc 
        tcttagcac tgttacagc tagtgtggt aattattac 
        tgatctaag gcgactgtt agacacaca cacgtaatg 
        aagttgctg ttatgttca actacttgt ggattatta 
        ggcatgtaa ccccttaat cggttcgtt tctcagtga 
        gcaaggtaa caaaattcc aaagctaga acacacaac 
        tcaaagccc ccgttgaaa accaacaca gaacagaat 
        acaaacagt acgggaaac tgacaaggc aataaggaa 
        ctaacagaa tcgagcctg cactggggc actctttac 
        ttttcaggt gatcaactg caggaatga caaacgtaa 
        aatccaatt gtttagtgt taaacctgc agaaatcgc 
        cccgaatca tttttaagt aaatggata tgaagatcg 
        gacagatca tgtcaaatc aaagacgaa ctcaaatag 
        ctgctacag cacaatcat tatatgaaa aaaaccaaa 
        tctcactac gccatcata tgtcatatg tttagtaaa 
        agtcatcaa attgcactt gaacgatat ttgtatata 
        agataatgt atgttgtat ttatagtaa ataacatgc 
        atcatataa taattacac tgcgatgtc aaatttctc 
        gtgtgaaag agcctactc agctttgct gctatcaaa 
        tctaaggtg ttatttacc agggtgtta ttcactgaa 
        aaagcccat agacaagtg caatatgcc catacatca 
        ttctattta ttgtgccaa ccacaaaac attaattat 
        taggaaaac aactcaaag agttatccc atgcattcg 
        cctcttcca ttgccattt cttgttcat aaaacaaat 
        ccagtaact gcaagtctt attctcttt cgctcttca 
        ttgtcatct ccttgtcga actaatatt tcaaaacgt 
        tcatttcag taaattcaa ttactacaa gttcatgcg 
        tcaaagcca gacaataaa tccaaacac actgactat 
        tatatgaga actaaagtt attctcttt gtgtaaaaa 
        taaaactgt gttaacgaa attatcatg cacttcttc 
        cagacttta gcaaaaata aattagtag agataaact 
        gcatttagt aaagattgg acaaaattg agaacaact 
        aattcatat caaatattt ctcaggaaa ttaactgct 
        attatctaa atggcattc ttttgtaaa catataaag 
        caacaatca attgttgtt agattcaac aaataatta 
        tttaaaacc aaa
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY