Status
Please wait ...
Gene information
General information
TC014340 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9385311..9389900
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014340
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10055

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014340 RA
      MQRRGGKRI RMDEPPPDY ENPMTPQVE PDGYGGYGH 
      PSLYGVEYT EQAGTSSSD GMFESPPTP GMRRITRSK 
      ARGQTNLPP PSYSPTGAG TPAPPKPRG RGRPPGRKN 
      TVEGSTEDE TSLYHYIKN SKVSLTNIV DEWIESYKV 
      NREAALIAL MQFFINAAG CKGRITQQM QATMEHAAI 
      IRRMTEEFD EESGEYPLI MAGQTWKKF RQNFCDFVQ 
      TLVKQCQYS IIYDQFLMD NVISLLTGL SDSQVRAFR 
      HTATLGAMK LMTALVDVA LTVSVNLDN TQRQYEAER 
      QKTREKRAS DRLEALLGK RQELEENMD EIKNMLTYM 
      FKSVFVHRY RDTLPEIRA IAMTEIGVW MHKFHANFL 
      DDSYLKYIG WTLHDKVGE VRLRCLQAL QPLYASEEL 
      KGKLELFTN KFKDRIVAM TLDKEYDVA VQAVRLVIS 
      ILKHHHEIL TDKDCEHVY ELVYSSHRA VAQAAGEFL 
      NERLFQPGD VDIGKTKRG KRRLPNTPF IRDLVQFFI 
      ESELHEHAA YLVDSLIES NSMMKDWEC MTDLLLEEP 
      GPQEEPLDN RQETSLIEI MVCCIKQAA TGEAPVGRG 
      PTRRVSSLR EMKQAGEDK QKLTEHFIV TLPPLLDKY 
      AADPEKLAN LLSIPQYFD LDLYTSGRQ EGSLQSLLV 
      KLKHIAQVH HEPEVLETL AKTLEILCT EGHSIYTRC 
      DVARSTIVD MIVVSYKEA IDDWRTLLL GEETPNADE 
      IFNVVSSLK KVSMFYACH NLNQWNLWS TLFQDVKDS 
      ESVLPPDAL KYSLSACFY CLLWGLRVL EIQHEGGGL 
      TAAAIQEMR QRLDEFIEA SQVLIRCSP HNSIKEEAY 
      VCLCDLLIV FSEQLSSTA PPLAELTCQ PDRNLQSLL 
      NEFVQSYVF VPEQNAEHD EHRIEELHK RRNFLAAYC 
      KLIVYNIMP TKAAADIFK HYVKYYNEY GDIIKATLS 
      KAREINKVN CALTMCLSL NMIFHEVQK LSTGKSTRQ 
      NEEFFALKE LAKRFALSF GLDAVKNRE AITALHRAG 
      ILFAVSGAD QPDDPTGPP PNLPFLEIL TEFTNKLLK 
      QDKRVVLTF LDRRITTGM PSSRGEDWQ PLLLYRNSL 
      LHGESDALP VTSKRAYGR KRKEIESDH EQDEVLSDQ 
      EFPGNLYG
    CDS sequence
    • >TC014340 RA
      atgcaacgt cgtggcggt aagagaatc cgaatggat 
      gagccgccc cccgactat gagaacccc atgacccct 
      caagttgag cccgatggt tacgggggc tacggccac 
      cccagttta tatggagtc gaatacacc gaacaagcg 
      ggcacatca tcatcagac ggcatgttt gaatcgccc 
      ccaactccc ggaatgcgc cgaattacc agatctaaa 
      gcaaggggg caaacaaat ctgccacca ccttcctat 
      tctccaact ggggctgga acaccagca ccgcccaaa 
      ccccgtggc aggggcaga ccccccggt cgcaaaaac 
      acggttgag gggagtaca gaagatgag acttctttg 
      taccactac atcaaaaac agcaaggtt tcacttacc 
      aatattgtg gatgagtgg atcgagagt tacaaggtc 
      aatagggag gcagctttg atcgcattg atgcagttt 
      tttataaac gctgctggt tgtaagggg agaataacg 
      caacagatg caagccacc atggaacat gcggctatt 
      atcaggaga atgaccgag gaatttgat gaagagtcg 
      ggcgaatac ccgcttatt atggccggt caaacgtgg 
      aaaaaattc aggcagaat ttttgcgat tttgtgcaa 
      actctggtc aaacagtgc cagtactcg atcatttac 
      gaccagttt ttaatggac aacgtgatt tcgttgctc 
      acggggctc tcagattcg caggtgcgt gcgttcagg 
      cacactgca accctcggc gcaatgaaa ctaatgacg 
      gctttagtg gacgttgct ttaacagtt tcggttaat 
      ctcgataat acgcaacgt cagtacgaa gccgagaga 
      cagaaaact cgcgaaaaa cgcgccagt gatcgtctt 
      gaagccctg cttgggaaa cggcaagag ctggaagaa 
      aatatggac gaaataaag aacatgttg acctacatg 
      tttaaatcg gttttcgtc catcgttat cgcgacact 
      ttgcccgaa atccgagca atagcaatg acggaaatc 
      ggtgtttgg atgcacaag ttccacgcg aatttcttg 
      gacgactct tatttaaaa tatataggg tggaccttg 
      catgataaa gtgggcgag gtgcgcttg aggtgcctc 
      caagccctt caaccgcta tatgctagt gaagagttg 
      aagggcaag ttggagttg ttcaccaat aaattcaaa 
      gacaggatc gttgcaatg actctggat aaagagtac 
      gacgtggcc gtgcaagct gtgcgactt gttatctca 
      atcctgaag catcatcac gaaatttta accgacaaa 
      gattgtgaa catgtgtac gagctggtt tactcctca 
      cacagagct gtggcgcaa gcagctggc gaatttcta 
      aacgaacgt ctcttccag ccgggagac gtggatata 
      ggtaaaacg aaaaggggc aaacgccgg ttgcccaac 
      acgccgttt atccgagac ttggtccag tttttcatc 
      gagtcggag ttgcacgaa catgcggct tatctagtc 
      gactcgctt atcgagtcc aacagtatg atgaaggac 
      tgggaatgc atgaccgat ttgttgctg gaggaaccc 
      ggaccacag gaggaacct ctggataat cgtcaagag 
      acgagtttg attgaaatt atggtgtgt tgcatcaaa 
      caagcggcc acgggggag gccccagtc gggaggggg 
      cccacaagg agggtttcg tcgttgcgt gaaatgaaa 
      caagccgga gaggacaag caaaaattg accgaacac 
      tttatagtg actttgcca ccgcttttg gataaatat 
      gcagccgat ccggagaaa ttggccaat ttgttgagt 
      attcctcag tattttgat cttgatttg tacaccagt 
      ggcaggcag gagggctcg cttcagtcg cttttggtc 
      aagctgaag cacatcgct caggtgcac catgaacca 
      gaagtgttg gagactctc gcaaaaact ctcgaaatt 
      ctctgcact gaaggccac tcaatttac actcgttgc 
      gacgtcgct agatcaaca attgtggac atgatcgtg 
      gtctcttac aaggaggca atcgacgat tggcgcact 
      ttactacta ggagaggaa acgccaaac gcggacgaa 
      atattcaac gtcgtcagt tcactaaaa aaagtttca 
      atgttctac gcttgtcac aacttgaac cagtggaat 
      ttatggtcc acacttttt caagacgtc aaagactcc 
      gaaagcgtg cttccacct gacgctctc aaatacagc 
      ctcagcgcc tgtttctac tgcctttta tggggtttg 
      agggttctg gagatccag catgagggc gggggcttg 
      actgcagcc gccatccaa gagatgcgt cagcgtctg 
      gacgaattt attgaagca tcgcaggtt ttaattcgg 
      tgttcgcca cataacagc atcaaggaa gaagcgtat 
      gtgtgtctg tgcgacttg ttgatcgtt tttagcgaa 
      cagttgtca agtacggca cctccactc gccgaactg 
      acttgccaa ccagacaga aatctccag tcgctttta 
      aacgaattt gtccaatct tacgttttt gtccccgaa 
      caaaatgcg gaacatgac gaacacaga atcgaggag 
      ttgcacaaa cggaggaat ttcctagct gcgtattgc 
      aaactaatc gtctacaac ataatgccg acgaaagcc 
      gccgcagac atcttcaaa cattacgtc aaatattac 
      aacgaatac ggcgacatt atcaaagca actttgagc 
      aaagcgcgc gaaattaat aaagttaac tgcgcccta 
      accatgtgt ttgagtctg aatatgatc ttccacgaa 
      gtgcagaaa ttatcaacg gggaaaagc acgcgtcag 
      aatgaggag ttctttgcg ctgaaagaa ttggcaaag 
      cgatttgca ctttcgttc ggtttggat gccgttaag 
      aatcgggaa gctataacg gcgctgcat cgagcgggg 
      attttgttt gctgtaagc ggggctgat caaccggat 
      gatcccacg ggaccacct ccgaattta ccgttcctg 
      gaaattttg actgagttc actaataag ttgctcaag 
      caggataaa agagtggtt ttgacgttt ctggatcgc 
      aggataact acaggaatg ccgagcagt aggggggaa 
      gactggcag ccgttgctg ctttatcgc aattctttg 
      ctgcatggt gagtcggac gctctgcca gttacgagc 
      aagagggcc tatgggagg aaaaggaag gaaattgag 
      tccgatcat gagcaagat gaggtgctg tctgatcag 
      gaatttccg ggaaatctt tatggataa
    mRNA sequence
    • >TC014340 RA
      gtctgataa attgaataa ctgaggaga atgaaatta 
      caaaaatat tacagcttt tttggctga tacatcgtt 
      tatatcatt tattttttg agtttaaac aaatacaaa 
      aattatcaa atcctacta taccggcat taaaatcgc 
      gtcccgctt tgcgtcttt ttcccgtaa ataaatatt 
      agttcgttt gtgattgat gtgaattaa aaaacactc 
      aaaatgcaa cgtcgtggc ggtaagaga atccgaatg 
      gatgagccg ccccccgac tatgagaac cccatgacc 
      cctcaagtt gagcccgat ggttacggg ggctacggc 
      caccccagt ttatatgga gtcgaatac accgaacaa 
      gcgggcaca tcatcatca gacggcatg tttgaatcg 
      cccccaact cccggaatg cgccgaatt accagatct 
      aaagcaagg gggcaaaca aatctgcca ccaccttcc 
      tattctcca actggggct ggaacacca gcaccgccc 
      aaaccccgt ggcaggggc agacccccc ggtcgcaaa 
      aacacggtt gaggggagt acagaagat gagacttct 
      ttgtaccac tacatcaaa aacagcaag gtttcactt 
      accaatatt gtggatgag tggatcgag agttacaag 
      gtcaatagg gaggcagct ttgatcgca ttgatgcag 
      ttttttata aacgctgct ggttgtaag gggagaata 
      acgcaacag atgcaagcc accatggaa catgcggct 
      attatcagg agaatgacc gaggaattt gatgaagag 
      tcgggcgaa tacccgctt attatggcc ggtcaaacg 
      tggaaaaaa ttcaggcag aatttttgc gattttgtg 
      caaactctg gtcaaacag tgccagtac tcgatcatt 
      tacgaccag tttttaatg gacaacgtg atttcgttg 
      ctcacgggg ctctcagat tcgcaggtg cgtgcgttc 
      aggcacact gcaaccctc ggcgcaatg aaactaatg 
      acggcttta gtggacgtt gctttaaca gtttcggtt 
      aatctcgat aatacgcaa cgtcagtac gaagccgag 
      agacagaaa actcgcgaa aaacgcgcc agtgatcgt 
      cttgaagcc ctgcttggg aaacggcaa gagctggaa 
      gaaaatatg gacgaaata aagaacatg ttgacctac 
      atgtttaaa tcggttttc gtccatcgt tatcgcgac 
      actttgccc gaaatccga gcaatagca atgacggaa 
      atcggtgtt tggatgcac aagttccac gcgaatttc 
      ttggacgac tcttattta aaatatata gggtggacc 
      ttgcatgat aaagtgggc gaggtgcgc ttgaggtgc 
      ctccaagcc cttcaaccg ctatatgct agtgaagag 
      ttgaagggc aagttggag ttgttcacc aataaattc 
      aaagacagg atcgttgca atgactctg gataaagag 
      tacgacgtg gccgtgcaa gctgtgcga cttgttatc 
      tcaatcctg aagcatcat cacgaaatt ttaaccgac 
      aaagattgt gaacatgtg tacgagctg gtttactcc 
      tcacacaga gctgtggcg caagcagct ggcgaattt 
      ctaaacgaa cgtctcttc cagccggga gacgtggat 
      ataggtaaa acgaaaagg ggcaaacgc cggttgccc 
      aacacgccg tttatccga gacttggtc cagtttttc 
      atcgagtcg gagttgcac gaacatgcg gcttatcta 
      gtcgactcg cttatcgag tccaacagt atgatgaag 
      gactgggaa tgcatgacc gatttgttg ctggaggaa 
      cccggacca caggaggaa cctctggat aatcgtcaa 
      gagacgagt ttgattgaa attatggtg tgttgcatc 
      aaacaagcg gccacgggg gaggcccca gtcgggagg 
      gggcccaca aggagggtt tcgtcgttg cgtgaaatg 
      aaacaagcc ggagaggac aagcaaaaa ttgaccgaa 
      cactttata gtgactttg ccaccgctt ttggataaa 
      tatgcagcc gatccggag aaattggcc aatttgttg 
      agtattcct cagtatttt gatcttgat ttgtacacc 
      agtggcagg caggagggc tcgcttcag tcgcttttg 
      gtcaagctg aagcacatc gctcaggtg caccatgaa 
      ccagaagtg ttggagact ctcgcaaaa actctcgaa 
      attctctgc actgaaggc cactcaatt tacactcgt 
      tgcgacgtc gctagatca acaattgtg gacatgatc 
      gtggtctct tacaaggag gcaatcgac gattggcgc 
      actttacta ctaggagag gaaacgcca aacgcggac 
      gaaatattc aacgtcgtc agttcacta aaaaaagtt 
      tcaatgttc tacgcttgt cacaacttg aaccagtgg 
      aatttatgg tccacactt tttcaagac gtcaaagac 
      tccgaaagc gtgcttcca cctgacgct ctcaaatac 
      agcctcagc gcctgtttc tactgcctt ttatggggt 
      ttgagggtt ctggagatc cagcatgag ggcgggggc 
      ttgactgca gccgccatc caagagatg cgtcagcgt 
      ctggacgaa tttattgaa gcatcgcag gttttaatt 
      cggtgttcg ccacataac agcatcaag gaagaagcg 
      tatgtgtgt ctgtgcgac ttgttgatc gtttttagc 
      gaacagttg tcaagtacg gcacctcca ctcgccgaa 
      ctgacttgc caaccagac agaaatctc cagtcgctt 
      ttaaacgaa tttgtccaa tcttacgtt tttgtcccc 
      gaacaaaat gcggaacat gacgaacac agaatcgag 
      gagttgcac aaacggagg aatttccta gctgcgtat 
      tgcaaacta atcgtctac aacataatg ccgacgaaa 
      gccgccgca gacatcttc aaacattac gtcaaatat 
      tacaacgaa tacggcgac attatcaaa gcaactttg 
      agcaaagcg cgcgaaatt aataaagtt aactgcgcc 
      ctaaccatg tgtttgagt ctgaatatg atcttccac 
      gaagtgcag aaattatca acggggaaa agcacgcgt 
      cagaatgag gagttcttt gcgctgaaa gaattggca 
      aagcgattt gcactttcg ttcggtttg gatgccgtt 
      aagaatcgg gaagctata acggcgctg catcgagcg 
      gggattttg tttgctgta agcggggct gatcaaccg 
      gatgatccc acgggacca cctccgaat ttaccgttc 
      ctggaaatt ttgactgag ttcactaat aagttgctc 
      aagcaggat aaaagagtg gttttgacg tttctggat 
      cgcaggata actacagga atgccgagc agtaggggg 
      gaagactgg cagccgttg ctgctttat cgcaattct 
      ttgctgcat ggtgagtcg gacgctctg ccagttacg 
      agcaagagg gcctatggg aggaaaagg aaggaaatt 
      gagtccgat catgagcaa gatgaggtg ctgtctgat 
      caggaattt ccgggaaat ctttatgga taagttgtt 
      gctaaaagc actatttaa tttatttac aaatggaat 
      tcctaatta tttggctct ttgatttct tgcgtcata 
      acaaggttt gattttgtt tgatataaa tataagtaa 
      tgaaagagt taatttatt agcgcactt ttttctatc 
      tactgtaaa tacattatg atgttctta tgaaacagt 
      cttattagg gtaagttat tttttgtgg aatcaagta 
      tttacgcag tttttaccg tttttacaa ttagttatt 
      ttttgaaac atatttata tttaagtta ataatggtc 
      ggtttatag aaagcccaa ttaaaattt aatgcgctg 
      ttcaggaat tttgcggat taaaataat acgcttcta 
      taaaccggc cctaagttg gtaataacc attacagat 
      tcttttgct gtaattgtt gctgtcata taatgtata 
      cctgctagt ttaaatgaa taaatgtat acttttatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY