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Gene information
General information
TC014336 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9288402..9289933
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014336
    iBeetle screen iB_10051

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 larval musculature lateral thinner - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    2

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014336 RA
      MVFLSRIYL AKIKRASRK YLKQGFRDS LKVFVSGGT 
      GGNGLPKFG GVGGQGGDV IAVGSDNIS LQDVFKRNQ 
      SKSYTAKAG RHSSHNFIL GPPGESLKF EVPVGVTVI 
      TELGKKIGE INNKGEELL LAKGGTGGN PKNGYLGTK 
      GQAYPVIFD LKLIADVGL VGFPNAGKS TLLKAISHA 
      KPKIASYPF TTVRPNVGI IQYKDLREI SMADLPGLI 
      EGAYANKGM GHKFLKHVE RTKLLLLVV DINGFQLSP 
      QYPHRSCLE TVLLLNKEL ELYNKDLLE KPSMLVINK 
      MDTENSHQK YSEIKDLLK NLTDAARQC PEEMRPENF 
      LKFSDVVAI SAKEKSDDV ELVKQRLRR MLDVLAQLE 
      NDDSKTYDV YKDIKDNLS EKGPSLV
    CDS sequence
    • >TC014336 RA
      atggtgttt ttatcaaga atttactta gcaaagata 
      aaaagagcg tcccgaaag tatctgaaa cagggcttt 
      cgggattcg ctgaaggtg tttgtgtca gggggtacc 
      ggaggaaac ggattacca aaatttggg ggtgtgggg 
      ggccaaggt ggtgatgta attgccgtg gggagtgat 
      aacatttcg ctacaagat gttttcaag cgaaatcaa 
      tcaaaaagt tacacagca aaggcaggg agacacagt 
      tctcacaat ttcatactt ggacctcct ggcgaaagc 
      ctgaaattt gaagtgcct gtcggcgtg actgtcatc 
      acagaattg gggaaaaaa attggtgaa ataaataac 
      aaaggtgaa gaactgttg cttgccaag ggcggtaca 
      gggggaaac cccaaaaat ggctattta gggacgaaa 
      gggcaggca tatcctgtt atttttgat ttgaaactg 
      attgcagat gtggggtta gttgggttt cccaatgcg 
      ggaaaaagc acccttctt aaagctatc tctcacgct 
      aaaccgaag attgctagt tatcctttt acaactgtg 
      aggcctaac gttggtatt atccagtac aaagattta 
      cgtgaaatc tctatggct gatcttccg ggtctcata 
      gaaggggct tacgctaac aaaggaatg gggcacaaa 
      ttcctaaaa cacgtagaa aggacgaaa ctattgcta 
      ctagtcgtt gacattaac gggtttcaa ttgagccca 
      caataccca cataggagc tgtctagaa actgtatta 
      cttttgaat aaggaactc gaattgtac aataaagat 
      ttactagaa aaaccatca atgttagtt atcaataag 
      atggatacg gaaaactcg caccaaaaa tatagtgaa 
      atcaaagat ttgttaaaa aatttaaca gatgcggca 
      agacagtgc ccagaggag atgagaccg gagaatttt 
      ttgaaattc agtgacgtg gtggcaatt tcagccaaa 
      gaaaaatca gacgatgtt gagcttgta aaacaacgg 
      cttagacgc atgttggac gttttagcc cagttggaa 
      aacgacgat tcaaaaact tacgatgtt tataaggat 
      ataaaggat aatttaagc gaaaagggc cctagtcta 
      gtgtaa
    mRNA sequence
    • >TC014336 RA
      gttatgctt tattaatat ttttttaaa atcgttctt 
      aattaggag caatggtgt ttttatcaa gaatttact 
      tagcaaaga taaaaagag cgtcccgaa agtatctga 
      aacagggct ttcgggatt cgctgaagg tgtttgtgt 
      cagggggta ccggaggaa acggattac caaaatttg 
      ggggtgtgg ggggccaag gtggtgatg taattgccg 
      tggggagtg ataacattt cgctacaag atgttttca 
      agcgaaatc aatcaaaaa gttacacag caaaggcag 
      ggagacaca gttctcaca atttcatac ttggacctc 
      ctggcgaaa gcctgaaat ttgaagtgc ctgtcggcg 
      tgactgtca tcacagaat tggggaaaa aaattggtg 
      aaataaata acaaaggtg aagaactgt tgcttgcca 
      agggcggta cagggggaa accccaaaa atggctatt 
      tagggacga aagggcagg catatcctg ttatttttg 
      atttgaaac tgattgcag atgtggggt tagttgggt 
      ttcccaatg cgggaaaaa gcacccttc ttaaagcta 
      tctctcacg ctaaaccga agattgcta gttatcctt 
      ttacaactg tgaggccta acgttggta ttatccagt 
      acaaagatt tacgtgaaa tctctatgg ctgatcttc 
      cgggtctca tagaagggg cttacgcta acaaaggaa 
      tggggcaca aattcctaa aacacgtag aaaggacga 
      aactattgc tactagtcg ttgacatta acgggtttc 
      aattgagcc cacaatacc cacatagga gctgtctag 
      aaactgtat tacttttga ataaggaac tcgaattgt 
      acaataaag atttactag aaaaaccat caatgttag 
      ttatcaata agatggata cggaaaact cgcaccaaa 
      aatatagtg aaatcaaag atttgttaa aaaatttaa 
      cagatgcgg caagacagt gcccagagg agatgagac 
      cggagaatt ttttgaaat tcagtgacg tggtggcaa 
      tttcagcca aagaaaaat cagacgatg ttgagcttg 
      taaaacaac ggcttagac gcatgttgg acgttttag 
      cccagttgg aaaacgacg attcaaaaa cttacgatg 
      tttataagg atataaagg ataatttaa gcgaaaagg 
      gccctagtc tagtgtaac taaaataaa ctttatttt 
      tctgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY