Status
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Gene information
General information
TC014326 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9097641..9116754
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014326
    Gene ontology

    GO terms for Tribolium

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      Status: {{ goTerm.status }}
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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10049

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014326 RA
      MTLRPGCCR ALALGFFIV WVVVHCAEA SSCKGCLNA 
      YQCDCKGIK GRPGSTGPP GLPAPPGDY GDIGPPGPS 
      GPKGEKGQG GEYGSPGER GSRGDDGER GLPGVEGRP 
      GYQGEAGPM GPPGLDGCN GTDGAQGAQ GARGLQGIR 
      GLPGPQGLQ GFRGEPGEG GGNSIGTKG DRGDGAYDG 
      PPGLDGFRG PTGPPGRIG EPGYEGIVG EQGFWGPPG 
      DIGDSIKGL KGQPGDLGD YGDVGEPGD DEPAPHYPQ 
      PSMYEWKGI KGEKGKGGY GGITGRRGY QGDKGFPGF 
      PGPKGRAGD KGEQGDDGP PGKQGRPGP FGPSGEKGD 
      KGAPGYEGT PGFDGETGP RGEDGNRGT PGVQGRSGP 
      PGKYDPFLD EIFEGSPGI QGPEGPVGE RGMSGIPGR 
      PGLSGPIGP PGPPGPPGI PGLAGPKGL TIKGERGTD 
      GLPGRPGNT GPPGLPGAM GILGEKGFT GQTVIGPPG 
      EPGYPGIPG FKGSRGERG GAGDVGPKG YPGVGVPIR 
      GPPGEFGPR GFPGSPGDV GLHGFDGFP GLKGRKGDD 
      CGVCLPGLP GQKGEAGEY GRNGRPGTI GYQGLPGPR 
      GPRGPQGKE GKEGERGLE GTPGTPGLQ GAPGTKGAR 
      GLLIFPDPS TKEAEAEKG EMGERGFPG LMGGDGEFG 
      PRGLPGDAG VPGFKGESG EIGTPGLPG LDGVPGING 
      IPGQKGEPH DIPKEYLRG DYGLTGVPG IKGVKGDKG 
      MMGLPGDSV SNIANVTGD IGYPGIQGE RGPPGRTGR 
      NGAPGPMGK EGLPGVPGS SPTGPPGEP GVRGYPGIP 
      GDAGPRGLP GMPGMPGLD GYPGTPGNK GERGDPGLR 
      ILNGEMGPQ GPPGIKGEP GDFGPDGYP GIPGIPGVK 
      GVRGPKGAP GFAGLPGAP GAKGMQGIM RPGFPGQPG 
      SLGLPGTPG RYGDTGQKG EPGFPGGPG YDGMKGEPG 
      LWGLRGQPG DEGIEGLPG HHGRAGLSG VQGLPGERG 
      DIGLPGLRG PPGPQGLLG PRGPKGLEG DRGPQGFPG 
      EMGIPGESG PRGDSGYPG IKGPKGESA ISGPKGEYG 
      EPGLIGEPG FDGEPGLPG PPGLPGDRG LDIYGPPGP 
      KGNLGIPGF PGRDGEVGL KGERGEGGI DGLRGAPGE 
      SGIRGAPGT PGWNGLNGT KGERGDYGS PGFPGNVGE 
      PGMEGEPGR MGRPGMPGD IGFPGISGP QGSVGPKGN 
      QGLPGLAPF GFGEKGDLG ETGFHGLPG NKGIRGDDG 
      FYGPPGLKG PTGDSGQPG LQGEPGYPG IKGTFGDSG 
      EPGPPGLPG RNPQPGEPG RPGFDGLPG RAGRPGQKG 
      APGEYGPDG PPGISGTPG FSMDGEKGD IGDSGYPGL 
      PGSPGLEGR PGIPGIPGV TGLKGPPGD YGFSAITFK 
      GERGEPGYR GAPGLNGTK GERGDFGVT GPPGPPGEE 
      GLPGFPGDV GPPGYPGAS GVKGPKGDR GDWSINALP 
      GPLGDAGLP GFDGLPGFQ GLPGEPGFP GLIGRKGMR 
      GDAGIDGLP GFEGPKGMA GLPGLAGLP GLQGARGAP 
      GVRGEPAPP PPAPKSRGF FFTRHSQSE LIPVCPRDT 
      VKLWDGYSL LHIMGNDKA MGQDLGTPA SCLRRFNTM 
      PFLFCNLND VCDYAQNNG YSYWLSTTE PMPAMMTPI 
      PAPEVRRYI SRCSVCESV TRVIAVHSQ STTIPQCPN 
      GWDELWAGY SFLMHVDAG AEGSGQSLV SPGSCLEEF 
      RPKPFIECH GHGRCNYYT TILSYWLAT IEDSAMFQK 
      PRSETLKAG YLTSRVSRC AVCIRRRYT SRTAIVTPS 
      RITRPTYGT PYRPPGYPP GSYRNLEEN RRRRLRQRL 
      RHRRPQNTP
    CDS sequence
    • >TC014326 RA
      atgacgctg cggccgggc tgttgccgg gccttagcc 
      ctcggcttt ttcatcgtc tgggtcgta gtgcactgc 
      gccgaagct tcgtcatgc aaaggctgt ctaaacgcc 
      tatcagtgc gactgcaag ggcattaag ggccggccc 
      gggagcacc ggacctcca gggctgcct gcaccgcct 
      ggagactac ggcgatatc ggtcctccg ggtccgtca 
      ggacctaaa ggagagaag ggacaaggc ggggagtat 
      ggtagtccc ggcgaaaga ggctccaga ggagatgat 
      ggtgaacga gggcttcct ggagtggaa ggacgtcct 
      ggttaccaa ggcgaggcc ggccccatg ggcccccca 
      ggccttgac ggttgtaat ggtaccgat ggtgcgcaa 
      ggggctcaa ggtgctcgc ggacttcaa gggatcagg 
      gggttacct ggccctcaa gggcttcaa ggatttaga 
      ggagagccc ggtgaaggt ggagggaat tcaattggg 
      actaaagga gatcgcggc gatggtgcc tacgatggt 
      cctcctgga ttagacgga tttcgaggt cccacagga 
      cctccaggt cgtataggt gaacctgga tatgaggga 
      atagtggga gagcaaggc ttttggggg cctcctggc 
      gacattggt gattcaatt aaaggccta aaaggtcaa 
      ccaggagac ttgggtgat tacggagat gtgggagaa 
      ccaggggat gacgagccc gcaccacat tacccccaa 
      ccatcgatg tatgaatgg aaaggcata aagggtgaa 
      aaaggaaag ggaggatat ggaggaata acaggacga 
      agaggatac cagggagat aaaggattt ccgggattt 
      ccgggtcct aaagggcga gcaggtgat aaaggcgag 
      caaggtgat gatggtcct cctggaaaa caaggtcgt 
      ccaggacct ttcggaccc tcgggtgaa aaaggagac 
      aagggcgcg ccggggtat gaaggaact ccgggattt 
      gacggtgaa acaggtcct agaggagaa gacgggaat 
      cgaggaacg cctggagtc caaggtcga agtggacct 
      cctggaaaa tacgaccca tttttggat gaaattttc 
      gaaggttct cctggaata caaggtccc gaaggtcca 
      gttggtgaa aggggcatg agtggtatt ccgggacgg 
      cctggatta tccggacct attggacct cccggccct 
      cccggcccc cctggtatt cctggttta gccggacca 
      aaaggactg acaattaag ggagaacgc ggcactgac 
      ggattaccc ggaaggccc gggaacacc ggtcctcct 
      gggctcccc ggagctatg ggtatcttg ggagagaaa 
      ggttttacg ggacaaacc gtcatcggg cctccgggt 
      gaaccagga tatcccggg atccccgga tttaaggga 
      tcgagagga gaaagaggc ggtgctggt gacgtgggt 
      cctaaaggt tacccaggg gttggtgtc cccattaga 
      gggccaccg ggagagttt ggcccgagg ggatttcca 
      ggatcgccg ggagacgtc ggtttacat ggattcgac 
      ggatttccg ggtttaaaa ggcagaaaa ggggatgat 
      tgtggggtg tgcctccca ggactaccg ggacaaaaa 
      ggtgaagca ggggagtac gggagaaat ggacgtcct 
      ggaacgatt gggtatcaa ggtttacct ggaccgcga 
      ggacctaga ggtcctcag gggaaagaa ggaaaggaa 
      ggtgaacga ggcttggaa ggtactccc ggcactcca 
      ggtctacag ggagctcca ggaacgaaa ggtgcaaga 
      ggattactg attttcccc gatccgtca actaaggaa 
      gctgaagct gaaaagggt gaaatggga gaaaggggt 
      tttccggga ttgatggga ggggatggg gagtttgga 
      ccgcgaggt ttaccagga gatgcgggg gttcctggg 
      tttaaaggc gaaagtggt gaaattggg actcctgga 
      ctgcctgga cttgatggg gtgcctgga ataaatggg 
      attccggga caaaaagga gaacctcac gacataccg 
      aaagaatat ttaagaggg gattatgga ttaacggga 
      gtaccgggt attaagggt gtaaaagga gataaaggg 
      atgatggga cttcctggc gattctgta tcaaatatt 
      gcaaatgta acaggcgac attggttat cccggaata 
      caaggagaa cgaggtcct ccaggccgg actggtaga 
      aatggcgcg ccaggccca atgggtaaa gaaggcttg 
      ccaggagtt cccgggagc agtccgaca ggtccccca 
      ggtgaacca ggtgtaaga ggttatcct ggcattcct 
      ggcgacgcc ggaccacgt ggtctcccc gggatgccc 
      ggtatgccc ggacttgat ggttatcct ggaacacca 
      ggaaacaaa ggcgaacga ggagatcca ggtttgcga 
      atcctgaat ggtgaaatg ggacctcaa ggaccaccc 
      ggaatcaag ggagaaccc ggcgatttc ggacctgat 
      ggatatcca ggaattcca ggaattcca ggtgttaaa 
      ggagtcaga ggacctaaa ggggcgcca ggttttgcg 
      ggtcttcct ggcgcaccc ggtgctaaa ggaatgcaa 
      ggaataatg cgaccagga tttcctggt cagccggga 
      agtctcgga cttccagga acacccgga agatatgga 
      gatactggg caaaaggga gaaccggga ttccctggt 
      ggtccagga tatgatggc atgaaggga gaaccggga 
      ttatgggga ttaagggga caaccagga gatgaagga 
      attgaaggt ttgccggga catcatgga agggctggt 
      ttgtccgga gttcaaggt ttacctgga gaaaggggc 
      gacattggt cttcctgga ttacgtggc cccccaggc 
      ccgcaggga cttttaggg ccaagagga ccaaaaggg 
      ttggaaggt gataggggc cctcaagga tttcctgga 
      gaaatggga attccggga gaatcggga cctagaggt 
      gatagtggt tatccggga ataaaaggc ccaaaaggt 
      gaatctgcg attagtgga cctaaagga gaatacgga 
      gaaccagga ttgattgga gaaccagga tttgatgga 
      gagcctgga ttacctgga cctccaggt ttaccagga 
      gatcgaggt ttagatatt tatggtcct cctggacct 
      aaaggaaat cttggaata cccggattc ccgggaaga 
      gacggagaa gtaggattg aagggagaa cgtggagag 
      ggcggaatt gatggctta cgaggcgct ccaggtgaa 
      tcgggaatt cgtggagcc cccggaaca cccggctgg 
      aatggtttg aatggcaca aaaggagaa agaggagat 
      tatggcagt cccggcttt cccggaaac gtaggggag 
      ccgggaatg gaaggagag ccgggacga atgggtcgt 
      cctggaatg ccgggcgat ataggattt ccgggaata 
      tctggacca caaggatcg gtcgggcct aagggaaat 
      caaggtctt cccgggtta gcaccattc ggattcggt 
      gaaaagggc gatttgggt gaaacaggt ttccatggc 
      ttacctggt aacaaggga atacgcgga gatgacggt 
      ttttatggc cccccaggt ctaaaaggg cctacgggt 
      gattctgga caaccagga ttacaaggc gaaccggga 
      tatccagga ataaaagga acattcggc gattccggc 
      gagcccgga cctccagga ttacccgga agaaacccg 
      caacctggc gaaccgggt cggcccgga tttgacggt 
      ttacctggc cgagcagga cgacccgga caaaaaggt 
      gctccagga gaatacgga cctgacggt cctcctggc 
      atctcaggc acacccggg ttcagcatg gacggcgaa 
      aagggcgat atcggagat tcaggatac ccaggcctg 
      cccggttca cccggactt gaaggtcgc cccggcata 
      cccggaatt cctggagtg actggtctt aaaggtcct 
      ccgggtgat tacggattt tccgctatc accttcaaa 
      ggtgagcgt ggcgagcct ggttatcgg ggcgctcct 
      ggattgaac ggaacgaaa ggagaacgt ggtgatttt 
      ggcgtaacg gggccacct ggaccgcca ggtgaagaa 
      ggtcttcca ggatttccc ggagacgtc ggccctcca 
      ggctaccct ggtgcgtca ggagtcaaa ggaccgaaa 
      ggagatcgg ggagactgg tccattaac gctcttccg 
      ggaccttta ggagacgcc ggtttaccc ggttttgac 
      ggactcccc ggatttcaa ggtttaccg ggcgaaccc 
      ggattccct ggtttgatc gggcggaaa gggatgcgt 
      ggtgatgcc gggattgac ggattacca gggtttgaa 
      ggacctaag ggcatggcg ggattaccg ggacttgca 
      ggattgcca ggtttacaa ggagccaga ggggcacct 
      ggggttagg ggcgagcca gcaccgcca ccaccggcc 
      cccaaaagt cgaggattt ttctttacg agacattcg 
      cagtcagaa ttaattcca gtgtgtcct agagatacg 
      gttaaattg tgggacggc tattcgttg ttacatatt 
      atggggaat gataaggcc atggggcaa gatttgggg 
      acgccagcg agttgtttg aggagattt aatactatg 
      ccgttcctg ttttgtaat ttgaacgat gtttgcgac 
      tacgcacag aacaatggg tacagttac tggttgtct 
      acgacagag ccaatgccg gcaatgatg acacccatc 
      ccagcacca gaagtcaga cgctatatt tcaagatgc 
      tccgtttgc gaatctgtc acacgtgtc atcgccgtc 
      cacagtcaa tcgacaacc atcccacag tgtcccaac 
      ggctgggac gaactctgg gccggatac agcttcctt 
      atgcacgtg gacgccggt gccgaaggc tcaggccaa 
      tccctcgtc tcccccggc tcatgcctc gaagagttc 
      cgccccaaa cccttcatc gaatgccac ggtcacggc 
      cgctgcaac tactacacc accatcctg tcctactgg 
      ctggccacc atagaggat tcagccatg ttccaaaaa 
      ccgcgttcg gagactctc aaagccggc tatttgacc 
      tcaagagtc agcagatgc gccgtttgc atccgcaga 
      cggtacacc agtcgcact gcaattgtc acccctagt 
      cgaatcaca agacccaca tacgggacc ccgtatcgg 
      cccccaggc tatccacca ggaagttac aggaacttg 
      gaagagaat aggaggagg cgtttgagg cagagattg 
      agacacagg agaccgcaa aatacgccg tga
    mRNA sequence
    • >TC014326 RA
      aggggccgc aaacagtgg caaaaattc gaaaaaagg 
      gacactcgg cgtagcccg ccggaatgt cttaagtca 
      caatagtgg gggtaagtc aagcagatt cagatggta 
      cagaacatg gagcagtag gctatgacg ctgcggccg 
      ggctgttgc cgggcctta gccctcggc tttttcatc 
      gtctgggtc gtagtgcac tgcgccgaa gcttcgtca 
      tgcaaaggc tgtctaaac gcctatcag tgcgactgc 
      aagggcatt aagggccgg cccgggagc accggacct 
      ccagggctg cctgcaccg cctggagac tacggcgat 
      atcggtcct ccgggtccg tcaggacct aaaggagag 
      aagggacaa ggcggggag tatggtagt cccggcgaa 
      agaggctcc agaggagat gatggtgaa cgagggctt 
      cctggagtg gaaggacgt cctggttac caaggcgag 
      gccggcccc atgggcccc ccaggcctt gacggttgt 
      aatggtacc gatggtgcg caaggggct caaggtgct 
      cgcggactt caagggatc agggggtta cctggccct 
      caagggctt caaggattt agaggagag cccggtgaa 
      ggtggaggg aattcaatt gggactaaa ggagatcgc 
      ggcgatggt gcctacgat ggtcctcct ggattagac 
      ggatttcga ggtcccaca ggacctcca ggtcgtata 
      ggtgaacct ggatatgag ggaatagtg ggagagcaa 
      ggcttttgg gggcctcct ggcgacatt ggtgattca 
      attaaaggc ctaaaaggt caaccagga gacttgggt 
      gattacgga gatgtggga gaaccaggg gatgacgag 
      cccgcacca cattacccc caaccatcg atgtatgaa 
      tggaaaggc ataaagggt gaaaaagga aagggagga 
      tatggagga ataacagga cgaagagga taccaggga 
      gataaagga tttccggga tttccgggt cctaaaggg 
      cgagcaggt gataaaggc gagcaaggt gatgatggt 
      cctcctgga aaacaaggt cgtccagga cctttcgga 
      ccctcgggt gaaaaagga gacaagggc gcgccgggg 
      tatgaagga actccggga tttgacggt gaaacaggt 
      cctagagga gaagacggg aatcgagga acgcctgga 
      gtccaaggt cgaagtgga cctcctgga aaatacgac 
      ccatttttg gatgaaatt ttcgaaggt tctcctgga 
      atacaaggt cccgaaggt ccagttggt gaaaggggc 
      atgagtggt attccggga cggcctgga ttatccgga 
      cctattgga cctcccggc cctcccggc ccccctggt 
      attcctggt ttagccgga ccaaaagga ctgacaatt 
      aagggagaa cgcggcact gacggatta cccggaagg 
      cccgggaac accggtcct cctgggctc cccggagct 
      atgggtatc ttgggagag aaaggtttt acgggacaa 
      accgtcatc gggcctccg ggtgaacca ggatatccc 
      gggatcccc ggatttaag ggatcgaga ggagaaaga 
      ggcggtgct ggtgacgtg ggtcctaaa ggttaccca 
      ggggttggt gtccccatt agagggcca ccgggagag 
      tttggcccg aggggattt ccaggatcg ccgggagac 
      gtcggttta catggattc gacggattt ccgggttta 
      aaaggcaga aaaggggat gattgtggg gtgtgcctc 
      ccaggacta ccgggacaa aaaggtgaa gcaggggag 
      tacgggaga aatggacgt cctggaacg attgggtat 
      caaggttta cctggaccg cgaggacct agaggtcct 
      caggggaaa gaaggaaag gaaggtgaa cgaggcttg 
      gaaggtact cccggcact ccaggtcta cagggagct 
      ccaggaacg aaaggtgca agaggatta ctgattttc 
      cccgatccg tcaactaag gaagctgaa gctgaaaag 
      ggtgaaatg ggagaaagg ggttttccg ggattgatg 
      ggaggggat ggggagttt ggaccgcga ggtttacca 
      ggagatgcg ggggttcct gggtttaaa ggcgaaagt 
      ggtgaaatt gggactcct ggactgcct ggacttgat 
      ggggtgcct ggaataaat gggattccg ggacaaaaa 
      ggagaacct cacgacata ccgaaagaa tatttaaga 
      ggggattat ggattaacg ggagtaccg ggtattaag 
      ggtgtaaaa ggagataaa gggatgatg ggacttcct 
      ggcgattct gtatcaaat attgcaaat gtaacaggc 
      gacattggt tatcccgga atacaagga gaacgaggt 
      cctccaggc cggactggt agaaatggc gcgccaggc 
      ccaatgggt aaagaaggc ttgccagga gttcccggg 
      agcagtccg acaggtccc ccaggtgaa ccaggtgta 
      agaggttat cctggcatt cctggcgac gccggacca 
      cgtggtctc cccgggatg cccggtatg cccggactt 
      gatggttat cctggaaca ccaggaaac aaaggcgaa 
      cgaggagat ccaggtttg cgaatcctg aatggtgaa 
      atgggacct caaggacca cccggaatc aagggagaa 
      cccggcgat ttcggacct gatggatat ccaggaatt 
      ccaggaatt ccaggtgtt aaaggagtc agaggacct 
      aaaggggcg ccaggtttt gcgggtctt cctggcgca 
      cccggtgct aaaggaatg caaggaata atgcgacca 
      ggatttcct ggtcagccg ggaagtctc ggacttcca 
      ggaacaccc ggaagatat ggagatact gggcaaaag 
      ggagaaccg ggattccct ggtggtcca ggatatgat 
      ggcatgaag ggagaaccg ggattatgg ggattaagg 
      ggacaacca ggagatgaa ggaattgaa ggtttgccg 
      ggacatcat ggaagggct ggtttgtcc ggagttcaa 
      ggtttacct ggagaaagg ggcgacatt ggtcttcct 
      ggattacgt ggcccccca ggcccgcag ggactttta 
      gggccaaga ggaccaaaa gggttggaa ggtgatagg 
      ggccctcaa ggatttcct ggagaaatg ggaattccg 
      ggagaatcg ggacctaga ggtgatagt ggttatccg 
      ggaataaaa ggcccaaaa ggtgaatct gcgattagt 
      ggacctaaa ggagaatac ggagaacca ggattgatt 
      ggagaacca ggatttgat ggagagcct ggattacct 
      ggacctcca ggtttacca ggagatcga ggtttagat 
      atttatggt cctcctgga cctaaagga aatcttgga 
      atacccgga ttcccggga agagacgga gaagtagga 
      ttgaaggga gaacgtgga gagggcgga attgatggc 
      ttacgaggc gctccaggt gaatcggga attcgtgga 
      gcccccgga acacccggc tggaatggt ttgaatggc 
      acaaaagga gaaagagga gattatggc agtcccggc 
      tttcccgga aacgtaggg gagccggga atggaagga 
      gagccggga cgaatgggt cgtcctgga atgccgggc 
      gatatagga tttccggga atatctgga ccacaagga 
      tcggtcggg cctaaggga aatcaaggt cttcccggg 
      ttagcacca ttcggattc ggtgaaaag ggcgatttg 
      ggtgaaaca ggtttccat ggcttacct ggtaacaag 
      ggaatacgc ggagatgac ggtttttat ggcccccca 
      ggtctaaaa gggcctacg ggtgattct ggacaacca 
      ggattacaa ggcgaaccg ggatatcca ggaataaaa 
      ggaacattc ggcgattcc ggcgagccc ggacctcca 
      ggattaccc ggaagaaac ccgcaacct ggcgaaccg 
      ggtcggccc ggatttgac ggtttacct ggccgagca 
      ggacgaccc ggacaaaaa ggtgctcca ggagaatac 
      ggacctgac ggtcctcct ggcatctca ggcacaccc 
      gggttcagc atggacggc gaaaagggc gatatcgga 
      gattcagga tacccaggc ctgcccggt tcacccgga 
      cttgaaggt cgccccggc atacccgga attcctgga 
      gtgactggt cttaaaggt cctccgggt gattacgga 
      ttttccgct atcaccttc aaaggtgag cgtggcgag 
      cctggttat cggggcgct cctggattg aacggaacg 
      aaaggagaa cgtggtgat tttggcgta acggggcca 
      cctggaccg ccaggtgaa gaaggtctt ccaggattt 
      cccggagac gtcggccct ccaggctac cctggtgcg 
      tcaggagtc aaaggaccg aaaggagat cggggagac 
      tggtccatt aacgctctt ccgggacct ttaggagac 
      gccggttta cccggtttt gacggactc cccggattt 
      caaggttta ccgggcgaa cccggattc cctggtttg 
      atcgggcgg aaagggatg cgtggtgat gccgggatt 
      gacggatta ccagggttt gaaggacct aagggcatg 
      gcgggatta ccgggactt gcaggattg ccaggttta 
      caaggagcc agaggggca cctggggtt aggggcgag 
      ccagcaccg ccaccaccg gcccccaaa agtcgagga 
      tttttcttt acgagacat tcgcagtca gaattaatt 
      ccagtgtgt cctagagat acggttaaa ttgtgggac 
      ggctattcg ttgttacat attatgggg aatgataag 
      gccatgggg caagatttg gggacgcca gcgagttgt 
      ttgaggaga tttaatact atgccgttc ctgttttgt 
      aatttgaac gatgtttgc gactacgca cagaacaat 
      gggtacagt tactggttg tctacgaca gagccaatg 
      ccggcaatg atgacaccc atcccagca ccagaagtc 
      agacgctat atttcaaga tgctccgtt tgcgaatct 
      gtcacacgt gtcatcgcc gtccacagt caatcgaca 
      accatccca cagtgtccc aacggctgg gacgaactc 
      tgggccgga tacagcttc cttatgcac gtggacgcc 
      ggtgccgaa ggctcaggc caatccctc gtctccccc 
      ggctcatgc ctcgaagag ttccgcccc aaacccttc 
      atcgaatgc cacggtcac ggccgctgc aactactac 
      accaccatc ctgtcctac tggctggcc accatagag 
      gattcagcc atgttccaa aaaccgcgt tcggagact 
      ctcaaagcc ggctatttg acctcaaga gtcagcaga 
      tgcgccgtt tgcatccgc agacggtac accagtcgc 
      actgcaatt gtcacccct agtcgaatc acaagaccc 
      acatacggg accccgtat cggccccca ggctatcca 
      ccaggaagt tacaggaac ttggaagag aataggagg 
      aggcgtttg aggcagaga ttgagacac aggagaccg 
      caaaatacg ccgtgatcg acgtgccat aacagttat 
      taactttgt aattatgtg taaattaat tgtaacata 
      gatttgatg ttaacattt gtccttgag aaggaacgt 
      acgccgtgt tcaaacaca aatcctatt agaatattt 
      tttacttgt aaggattta ggttcttaa ttttattta 
      agcgattac gacagtggc tctttgtat agaatttat 
      ttttttaat acttgaatt cttgcttgt ttttgatct 
      tttttatct caaaggtgc taattagtt ttaaggctt 
      acgacagga aaatgtgat tcatatttt atgtgcctt 
      tctattaaa cactgccaa atgattttg taatgtaac 
      aaatgaata aatcataaa aacgatg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY