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Gene information
General information
TC013478 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 9034907..9036679
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC013478
      iBeetle screen iB_10047

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC013478 RA
        MPPAAKRHC SVILFDLKG QNRLGALQT LLKISSTNF 
        FSRSKDLTK LILLNSDKT ENRLNARHG GYEHVNEVT 
        KDVLSYNPK KLHDFVESS GVGEGNWLE GLHVAAANL 
        VDEFEGIKG IITYQLLFI TDLGSPLSK VDNGLIDKI 
        ARLLDRLGA FLYVVGPEI EPPFTIKTP ENIKTWMEN 
        LQTNDPNIA TIKTLISKI QNGVVCGPK IGTNLFFSY 
        RNSGGSQPW NVPLGIGTN INIPAQTMK ILNDIVQCK 
        LVSAQGAIR PSTWIDAED ESIEFDASE VISGVSRHG 
        KFVPVNNKM FQVSSSRSF SVLCFTDAA NVPEHLMRG 
        GGCYSVLPN ASLEKNDAF NCLIDCLAQ QNKYVIARR 
        VYNNNYVPK IVVLVPKPD YNPKCFVLT ALPFADDVR 
        LNCKEQKVD LAPKIEENK SVYKLLDGL DTDNENSVK 
        KAPLDIKMV QNVNVQRIV NAAVDKLLD KHINLEETD 
        LDLMELPSG EYMEELKNS WPERKIEEV KKNEEPAAD 
        ISDDDFDWE
      CDS sequence
      • >TC013478 RA
        atgcctcca gctgctaaa aggcactgt tcggttatt 
        ttatttgac cttaaaggt caaaaccgt ttaggggcg 
        ctccagacg ctactgaaa atttcaagt acgaatttt 
        ttctcgcgt tccaaggat ttaaccaaa ttgattttg 
        ttgaattcg gacaagact gaaaatcgt ttgaacgcc 
        cgacacggg gggtacgaa cacgtaaat gaagttacg 
        aaggatgtt ttgtcctat aatcccaaa aaactgcat 
        gattttgtc gaaagcagt ggcgtcggt gagggcaat 
        tggttggaa ggtctgcac gtggctgcc gcaaacttg 
        gtcgatgag tttgaagga atcaaggga attatcact 
        tatcagttg ctttttatc acagacttg ggttcgccc 
        ctgtctaaa gttgataac ggtttaatt gacaaaata 
        gcccggcta ctggaccgg cttggggct tttttgtat 
        gtcgtgggc cccgaaatc gagccccct ttcactata 
        aaaacgccc gaaaacatc aaaacctgg atggagaat 
        ttgcaaaca aacgaccca aacatcgcc accattaaa 
        accctcatt tcgaaaatt caaaacggc gttgtttgt 
        ggtcccaaa atcgggacg aatttattt ttttcgtac 
        cgcaactcg ggcgggagt cagccctgg aacgtccca 
        ttgggcatt ggcacaaac atcaacatt ccggcacaa 
        actatgaaa atactaaac gatatcgtc cagtgcaag 
        ttggtttca gcacaagga gcgatcagg ccttcaact 
        tggattgac gctgaggat gagtctatt gaattcgac 
        gccagtgag gtaattagc ggtgtttcg cgacacggg 
        aaattcgtc ccagtcaac aacaaaatg tttcaagtc 
        tcaagttcg cgcagtttc agcgttttg tgtttcaca 
        gacgcggca aatgtgcct gagcatttg atgcgaggc 
        ggcggttgt tacagcgtt ttgcccaac gctagtttg 
        gaaaaaaat gacgctttt aattgtttg attgattgc 
        ctggcgcaa caaaataaa tacgttatt gcccgacgt 
        gtctataat aataattat gtgccgaag attgtggtt 
        ttggtgccg aaaccggat tataatccg aaatgtttt 
        gttctgacg gcgttgccg ttcgctgat gatgttagg 
        ttgaattgc aaagaacag aaagttgat ttggcgccg 
        aaaattgaa gaaaacaag agcgtttac aaactttta 
        gatggttta gatacggat aatgaaaat tcggttaag 
        aaggcgcca ctcgacatt aaaatggtg cagaacgtg 
        aacgtccaa agaattgtt aatgcagcc gtggataaa 
        ttgttggat aaacatata aatttggaa gaaaccgat 
        ttagatctg atggagctt ccttctggg gaatatatg 
        gaagaacta aagaatagt tggcccgaa cgtaaaatc 
        gaagaagtt aagaagaac gaagaacct gcggctgac 
        atctctgat gatgatttt gattgggaa taa
      mRNA sequence
      • >TC013478 RA
        gcaaaacaa tgcctccag ctgctaaaa ggcactgtt 
        cggttattt tatttgacc ttaaaggtc aaaaccgtt 
        taggggcgc tccagacgc tactgaaaa tttcaagta 
        cgaattttt tctcgcgtt ccaaggatt taaccaaat 
        tgattttgt tgaattcgg acaagactg aaaatcgtt 
        tgaacgccc gacacgggg ggtacgaac acgtaaatg 
        aagttacga aggatgttt tgtcctata atcccaaaa 
        aactgcatg attttgtcg aaagcagtg gcgtcggtg 
        agggcaatt ggttggaag gtctgcacg tggctgccg 
        caaacttgg tcgatgagt ttgaaggaa tcaagggaa 
        ttatcactt atcagttgc tttttatca cagacttgg 
        gttcgcccc tgtctaaag ttgataacg gtttaattg 
        acaaaatag cccggctac tggaccggc ttggggctt 
        ttttgtatg tcgtgggcc ccgaaatcg agccccctt 
        tcactataa aaacgcccg aaaacatca aaacctgga 
        tggagaatt tgcaaacaa acgacccaa acatcgcca 
        ccattaaaa ccctcattt cgaaaattc aaaacggcg 
        ttgtttgtg gtcccaaaa tcgggacga atttatttt 
        tttcgtacc gcaactcgg gcgggagtc agccctgga 
        acgtcccat tgggcattg gcacaaaca tcaacattc 
        cggcacaaa ctatgaaaa tactaaacg atatcgtcc 
        agtgcaagt tggtttcag cacaaggag cgatcaggc 
        cttcaactt ggattgacg ctgaggatg agtctattg 
        aattcgacg ccagtgagg taattagcg gtgtttcgc 
        gacacggga aattcgtcc cagtcaaca acaaaatgt 
        ttcaagtct caagttcgc gcagtttca gcgttttgt 
        gtttcacag acgcggcaa atgtgcctg agcatttga 
        tgcgaggcg gcggttgtt acagcgttt tgcccaacg 
        ctagtttgg aaaaaaatg acgctttta attgtttga 
        ttgattgcc tggcgcaac aaaataaat acgttattg 
        cccgacgtg tctataata ataattatg tgccgaaga 
        ttgtggttt tggtgccga aaccggatt ataatccga 
        aatgttttg ttctgacgg cgttgccgt tcgctgatg 
        atgttaggt tgaattgca aagaacaga aagttgatt 
        tggcgccga aaattgaag aaaacaaga gcgtttaca 
        aacttttag atggtttag atacggata atgaaaatt 
        cggttaaga aggcgccac tcgacatta aaatggtgc 
        agaacgtga acgtccaaa gaattgtta atgcagccg 
        tggataaat tgttggata aacatataa atttggaag 
        aaaccgatt tagatctga tggagcttc cttctgggg 
        aatatatgg aagaactaa agaatagtt ggcccgaac 
        gtaaaatcg aagaagtta agaagaacg aagaacctg 
        cggctgaca tctctgatg atgattttg attgggaat 
        aatgtgtgt tttcacttg actattaaa cttactctt 
        gcgtt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY