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Gene information
General information
TC013484 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8966942..8973279
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013484
    iBeetle screen iB_10046

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    adult nutritional state bad (cachectic) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    adult nutritional state potentially bad (cachectic) - Number of animals showing the phenotype: 3 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013484 RA
      MVIKSDKTL SPFAAPAER RSSLRQALP QILAVCVKN 
      VLLLGFGMT LGFPTILIP SLSGSDPNE PISLGQEAI 
      SWIGSINLI CVPIGCLLS GAATQPIGR RRAMQLVNI 
      PFLTAWLLF YFSNDVWQI FLALCITGV TGGLLEAPV 
      LTYVAEITQ PHLRGMLSS TSTMAVILG VLVQFLLGT 
      FLNWRLVTL CNCVFPIVA FVLLIFVPE TPIWLISKN 
      RYLDARKSL AWLRGWTSL NEIELEFQD LCKQLGKAG 
      EIGIDNPEN SPNQKLSKL EHLKLFTRK NFFWPYSLV 
      ALTFFLGHF NGMNALQTY AIKIFAAVK SPIDKYYAT 
      VILGVVELL GCVACVTLV HFTGKRVIN LISLLGSGV 
      CFFIVAIYT YISDIKHLE GPQHSDLKH NWIPTFFLI 
      TAAFLSYVG IRILPWILT GEVFSNETR ATASGLSGA 
      IGYIFGFLA NKIFLSMVT VFTLPGTFW FNSSVSILG 
      AILLYFVLP ETEGKTLYD ITEHFQGNT KLDNKVQRN 
      GIDNKAFEL EEILTSGTS HPTLNNPIF LFQTLTYVA 
      EITLPSLRG ILSSTSGVA VICGILAQF LLGTFLNWR 
      IVALVSGVV PIVSFFLLF FVPESPYWL ILKNRHEEA 
      RKCIAWLRG WTTIEDIEP EFAELCKQI SSTVSEKPT 
      IIEKLKLFT KKNFFWPFL VVSFAFFMS QFSGTTPLQ 
      IYAVKIFAT LKAPIDEYY ATVAMGVAE VLGCVLSTC 
      LVHYTGKRK MNFFSLISC GLCFLIVAT YAYLHNINQ 
      LEKFSSSSN RSGDTSWLP MVFLVTAAF CTHTGIKLL 
      PWMLIGEVY SNETRATAS GFSGAVSYV FGFISIKIF 
      LYLVNWITL PGTFWFYCI MCFIGTVVL YFILPETEG 
      KTLFEITEH FASNSKLSN KVTRIKDIR KGEINNAYV 
      SNDETRTLT YVAEITQPS LRGILASTS TVSVISGIL 
      AQFLLGTFL AWRNVALVS CIVPFCSFT LLFFVPESP 
      HWLISKNRF LDARQSLAW LRGWTDLTS IEPEFKELS 
      QQITTRLER NSPSWKLYL RKNFLWPYS LVSFTFLLG 
      HFSGMTTLQ TYAVKIFSD LRAPIDKYY ATIFLGVAE 
      VCGCLLSAC LIHYVGKRV MNFFSLLGC GSCFLVTAF 
      YAQSSETSD SNWIPMTLL IGAAFFTHA GIRILPWML 
      IGEVYSNDT RATASGLSG GLSYIFGFI ANKIFLKMV 
      AFLTLPGTF WLYCGFCFG GALILYFIL PETEGKTLF 
      EIQEHFCGN VKMDNKVGR KRNSQGAGE VNKGFAGDE 
      EDGKIESKL
    CDS sequence
    • >TC013484 RA
      atggtcatt aaaagtgat aaaaccctc tcgcccttc 
      gcggccccg gccgaacgc cggtcaagc ctccgccaa 
      gccctcccc cagatcctg gccgtttgc gtcaaaaac 
      gtcctcctc ctgggcttt ggcatgact cttggcttc 
      cccaccatc ctcatcccc agcctttcg gggagcgac 
      cccaacgag cccatctcg ctgggccag gaggccatc 
      tcttggatc ggctccatc aacctcatc tgtgtcccc 
      atcgggtgc ctcctctca ggggccgcc acccagccc 
      atcggccgg aggagagcc atgcagctc gtaaacatc 
      cccttcctg accgcctgg ctccttttc tacttctcc 
      aacgacgtg tggcaaatt ttcctcgcc ttgtgcatc 
      accggagtc accggaggc ttgctcgaa gcccccgtg 
      cttacatac gttgctgaa ataacacaa ccacactta 
      cggggaatg ctctcttcg actagcacc atggcggtc 
      atcttgggg gtcttggtt cagtttctc ttgggcact 
      tttcttaac tggagactt gtaacgctc tgtaactgt 
      gtttttccg atcgtggcg ttcgttctt ttgattttc 
      gtgccggaa actcccatt tggttgatt tccaaaaac 
      cggtatctc gatgcccgg aagagtctg gcatggcta 
      aggggctgg acaagtctc aacgaaatc gagctcgaa 
      ttccaggat ttgtgcaaa caattgggc aaagcgggc 
      gaaatcggc attgacaac ccggaaaat tcaccaaac 
      cagaaatta tcaaaactg gagcatttg aagcttttc 
      acgaggaaa aacttcttc tggccctat tctctagtc 
      gctttgacg tttttcctc ggacatttc aacggcatg 
      aacgcttta caaacctac gcgatcaaa attttcgcc 
      gcggttaag tcaccaatc gacaaatat tacgccacg 
      gttattttg ggggttgtg gagttgttg gggtgtgtt 
      gcatgcgtt acacttgtc catttcacg gggaaaagg 
      gtaataaac ttaatttcg ttgctgggt tcgggtgtg 
      tgtttcttc atagttgcc atctacacc tacatttct 
      gatatcaaa catttggag gggccccaa cactcagat 
      ctcaaacat aactggatc cccacattc tttctcatc 
      actgccgct tttttgtca tatgttggt atccgaatc 
      ttgccctgg attttgacc ggagaagtt ttctcgaac 
      gaaacccga gccacggct tccggtctt tcgggcgcc 
      atcggctac atttttggg ttcttagcc aataagatt 
      ttcctctca atggtcacc gtgttcacg cttccgggc 
      actttctgg ttcaattct agtgttagc atcctcggt 
      gccatttta ctctatttc gttctgccg gaaacggag 
      ggaaagact ttatacgac atcactgaa cactttcag 
      ggtaacaca aaactagac aacaaagtc caaagaaac 
      ggaatcgac aataaagcc ttcgaactc gaagaaata 
      ctaacctcg ggcacgtct cacccgact ctgaataac 
      ccgattttt ctcttccag accctcact tacgttgca 
      gaaatcact ctacctagt cttagaggg attttgtcg 
      tcgaccagt ggcgtggct gtaatttgc ggaatcttg 
      gcccaattc ttactagga accttcctg aactggaga 
      attgtagct ctagtgagc ggagttgtc ccaattgtg 
      tctttcttt ctcctgttt ttcgtcccc gagagcccc 
      tattggctc atattgaaa aaccggcat gaggaagcc 
      cgtaaatgc atcgcatgg ttgcgtgga tggacaaca 
      atcgaagac attgaacca gaatttgcc gaactgtgc 
      aaacaaatc agttcaaca gtgagcgaa aaaccaacc 
      atcatcgag aagttaaaa ctcttcacg aagaaaaac 
      ttcttttgg cccttcttg gttgtgtct tttgccttc 
      ttcatgagc cagttcagt ggtaccact ccactgcaa 
      atctacgcg gtcaaaatt ttcgcaacg ttgaaggct 
      cccatcgat gagtactac gcaactgtg gcgatgggc 
      gtcgctgag gtccttggc tgtgtttta agtacttgt 
      cttgtgcat tataccggc aaacgaaaa atgaatttt 
      ttctcgttg attagttgc ggtctctgc ttcctcatc 
      gttgcgact tatgcctac ttgcataac attaatcag 
      ttggagaaa ttttcttca agtagtaat cgttccggt 
      gatacgagc tggttgccg atggttttt ctcgtaact 
      gcggctttt tgtacccat actgggatc aagctgttg 
      ccgtggatg ctcatcgga gaggtttat tcgaacgaa 
      actagagcc acagcgtcc gggttctcg ggcgctgtg 
      agctacgtt ttcgggttt atttcaatc aagattttc 
      ctgtatttg gtcaactgg atcacgctc cctggcact 
      ttctggttt tattgtatc atgtgtttt attggtacc 
      gttgttctt tacttcatc ttgcccgaa actgagggg 
      aaaacactc ttcgaaatc accgaacac tttgccagt 
      aattctaaa ctaagtaac aaagtaaca cgaatcaaa 
      gacatccgc aaaggcgaa ataaataac gcttatgtt 
      tcaaatgac gaaactcgg actttgacg tacgttgct 
      gaaatcact cagccgagt ctcagaggg attttagca 
      tccacaagc acagtctct gtgatctca ggtatctta 
      gcccaattc ttactagga acatttctg gcgtggcga 
      aacgtagct ttggttagt tgtatcgtc cctttttgc 
      tccttcact ctcctcttc ttcgtcccg gaaagtccc 
      cattggtta atatcgaaa aaccgcttc ctggacgcc 
      cgccaaagc ctggcctgg ctgcgaggc tggaccgac 
      ttgacctcc atcgagccc gaattcaag gaactctcc 
      caacaaatc acgacccga ctggaacgc aactccccc 
      tcctggaag ctttacctc cgaaagaac ttcctctgg 
      ccttactcc ctagtctcc ttcacgttc ctcctgggc 
      cacttttca ggaatgacc accctccag acgtacgct 
      gttaaaatt ttctccgac ttgagagcc cccattgac 
      aagtactac gcaacgatt ttcctgggg gtggccgaa 
      gtctgcggc tgcctcctc agcgcgtgt ttgatccac 
      tacgtgggg aaacgagtg atgaatttt ttttcgctc 
      ctgggctgc ggctcctgt ttcctcgtt acagctttc 
      tacgcacaa tcgagcgaa accagcgac tccaactgg 
      atccccatg actctgctc atcggagcc gcttttttc 
      acacacgct gggataagg atcctcccg tggatgctc 
      atcggagaa gtgtactcg aacgataca agagccacg 
      gcttcggga ctttcgggt ggtctgagc tacattttt 
      gggttcatt gcgaacaaa atattcctc aaaatggtg 
      gcgtttttg acgcttccc gggacgttt tggctttat 
      tgtggcttt tgtttcggg ggagctctt atcctgtac 
      tttatttta ccagaaacg gaaggcaag actttgttc 
      gaaatccag gaacacttt tgcgggaac gtaaaaatg 
      gacaataaa gttgggcga aaaaggaac tcccaaggg 
      gccggggaa gtcaataaa gggtttgcg ggagatgag 
      gaagatggc aaaatcgag agcaaatta taa
    mRNA sequence
    • >TC013484 RA
      tgtacaaca cagcaaaat atttgttta ttttatttt 
      taaacaaac aaaaggaat gtaaacgac cgtttatta 
      gaataaagc caatcagta ttgaactgt gatatgtgt 
      actaaacaa gaattatca aagtagtgc aattggaac 
      aataaacca gtgttcata taccaaatc tttagtgca 
      caatggtca ttaaaagtg ataaaaccc tctcgccct 
      tcgcggccc cggccgaac gccggtcaa gcctccgcc 
      aagccctcc cccagatcc tggccgttt gcgtcaaaa 
      acgtcctcc tcctgggct ttggcatga ctcttggct 
      tccccacca tcctcatcc ccagccttt cggggagcg 
      accccaacg agcccatct cgctgggcc aggaggcca 
      tctcttgga tcggctcca tcaacctca tctgtgtcc 
      ccatcgggt gcctcctct caggggccg ccacccagc 
      ccatcggcc ggaggagag ccatgcagc tcgtaaaca 
      tccccttcc tgaccgcct ggctccttt tctacttct 
      ccaacgacg tgtggcaaa ttttcctcg ccttgtgca 
      tcaccggag tcaccggag gcttgctcg aagcccccg 
      tgcttacat acgttgctg aaataacac aaccacact 
      tacggggaa tgctctctt cgactagca ccatggcgg 
      tcatcttgg gggtcttgg ttcagtttc tcttgggca 
      cttttctta actggagac ttgtaacgc tctgtaact 
      gtgtttttc cgatcgtgg cgttcgttc ttttgattt 
      tcgtgccgg aaactccca tttggttga tttccaaaa 
      accggtatc tcgatgccc ggaagagtc tggcatggc 
      taaggggct ggacaagtc tcaacgaaa tcgagctcg 
      aattccagg atttgtgca aacaattgg gcaaagcgg 
      gcgaaatcg gcattgaca acccggaaa attcaccaa 
      accagaaat tatcaaaac tggagcatt tgaagcttt 
      tcacgagga aaaacttct tctggccct attctctag 
      tcgctttga cgtttttcc tcggacatt tcaacggca 
      tgaacgctt tacaaacct acgcgatca aaattttcg 
      ccgcggtta agtcaccaa tcgacaaat attacgcca 
      cggttattt tgggggttg tggagttgt tggggtgtg 
      ttgcatgcg ttacacttg tccatttca cggggaaaa 
      gggtaataa acttaattt cgttgctgg gttcgggtg 
      tgtgtttct tcatagttg ccatctaca cctacattt 
      ctgatatca aacatttgg aggggcccc aacactcag 
      atctcaaac ataactgga tccccacat tctttctca 
      tcactgccg cttttttgt catatgttg gtatccgaa 
      tcttgccct ggattttga ccggagaag ttttctcga 
      acgaaaccc gagccacgg cttccggtc tttcgggcg 
      ccatcggct acatttttg ggttcttag ccaataaga 
      ttttcctct caatggtca ccgtgttca cgcttccgg 
      gcactttct ggttcaatt ctagtgtta gcatcctcg 
      gtgccattt tactctatt tcgttctgc cggaaacgg 
      agggaaaga ctttatacg acatcactg aacactttc 
      agggtaaca caaaactag acaacaaag tccaaagaa 
      acggaatcg acaataaag ccttcgaac tcgaagaaa 
      tactaacct cgggcacgt ctcacccga ctctgaata 
      acccgattt ttctcttcc agaccctca cttacgttg 
      cagaaatca ctctaccta gtcttagag ggattttgt 
      cgtcgacca gtggcgtgg ctgtaattt gcggaatct 
      tggcccaat tcttactag gaaccttcc tgaactgga 
      gaattgtag ctctagtga gcggagttg tcccaattg 
      tgtctttct ttctcctgt ttttcgtcc ccgagagcc 
      cctattggc tcatattga aaaaccggc atgaggaag 
      cccgtaaat gcatcgcat ggttgcgtg gatggacaa 
      caatcgaag acattgaac cagaatttg ccgaactgt 
      gcaaacaaa tcagttcaa cagtgagcg aaaaaccaa 
      ccatcatcg agaagttaa aactcttca cgaagaaaa 
      acttctttt ggcccttct tggttgtgt cttttgcct 
      tcttcatga gccagttca gtggtacca ctccactgc 
      aaatctacg cggtcaaaa ttttcgcaa cgttgaagg 
      ctcccatcg atgagtact acgcaactg tggcgatgg 
      gcgtcgctg aggtccttg gctgtgttt taagtactt 
      gtcttgtgc attataccg gcaaacgaa aaatgaatt 
      ttttctcgt tgattagtt gcggtctct gcttcctca 
      tcgttgcga cttatgcct acttgcata acattaatc 
      agttggaga aattttctt caagtagta atcgttccg 
      gtgatacga gctggttgc cgatggttt ttctcgtaa 
      ctgcggctt tttgtaccc atactggga tcaagctgt 
      tgccgtgga tgctcatcg gagaggttt attcgaacg 
      aaactagag ccacagcgt ccgggttct cgggcgctg 
      tgagctacg ttttcgggt ttatttcaa tcaagattt 
      tcctgtatt tggtcaact ggatcacgc tccctggca 
      ctttctggt tttattgta tcatgtgtt ttattggta 
      ccgttgttc tttacttca tcttgcccg aaactgagg 
      ggaaaacac tcttcgaaa tcaccgaac actttgcca 
      gtaattcta aactaagta acaaagtaa cacgaatca 
      aagacatcc gcaaaggcg aaataaata acgcttatg 
      tttcaaatg acgaaactc ggactttga cgtacgttg 
      ctgaaatca ctcagccga gtctcagag ggattttag 
      catccacaa gcacagtct ctgtgatct caggtatct 
      tagcccaat tcttactag gaacatttc tggcgtggc 
      gaaacgtag ctttggtta gttgtatcg tcccttttt 
      gctccttca ctctcctct tcttcgtcc cggaaagtc 
      cccattggt taatatcga aaaaccgct tcctggacg 
      cccgccaaa gcctggcct ggctgcgag gctggaccg 
      acttgacct ccatcgagc ccgaattca aggaactct 
      cccaacaaa tcacgaccc gactggaac gcaactccc 
      cctcctgga agctttacc tccgaaaga acttcctct 
      ggccttact ccctagtct ccttcacgt tcctcctgg 
      gccactttt caggaatga ccaccctcc agacgtacg 
      ctgttaaaa ttttctccg acttgagag cccccattg 
      acaagtact acgcaacga ttttcctgg gggtggccg 
      aagtctgcg gctgcctcc tcagcgcgt gtttgatcc 
      actacgtgg ggaaacgag tgatgaatt ttttttcgc 
      tcctgggct gcggctcct gtttcctcg ttacagctt 
      tctacgcac aatcgagcg aaaccagcg actccaact 
      ggatcccca tgactctgc tcatcggag ccgcttttt 
      tcacacacg ctgggataa ggatcctcc cgtggatgc 
      tcatcggag aagtgtact cgaacgata caagagcca 
      cggcttcgg gactttcgg gtggtctga gctacattt 
      ttgggttca ttgcgaaca aaatattcc tcaaaatgg 
      tggcgtttt tgacgcttc ccgggacgt tttggcttt 
      attgtggct tttgtttcg ggggagctc ttatcctgt 
      actttattt taccagaaa cggaaggca agactttgt 
      tcgaaatcc aggaacact tttgcggga acgtaaaaa 
      tggacaata aagttgggc gaaaaagga actcccaag 
      gggccgggg aagtcaata aagggtttg cgggagatg 
      aggaagatg gcaaaatcg agagcaaat tataacaca 
      attgaaaag tattgtaac acatatctt tatttataa 
      taaaatatt gttatctat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY