Status
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Gene information
General information
TC033126
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC013488,TC013487
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8949702..8956299
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033126
    iBeetle screen iB_10045

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    cuticle remnants strongly fragmented (cuticle crumbs) - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033126 RA
      MIGIFADSL GRKQSLQYN NSMVFAAAA VMFFSKKAK 
      SYIMLIMGR FIAGINSGL NMGLCPIYL IEIAPDRIR 
      GAIGSLYQV VITCSIVVS QIAGVLLGS EESWPYLFL 
      LPIIPALFQ LALLPFCPE SPKFLITRL KDRKAHRAL 
      MLLRSQQDV VDEFEQMKE EDSLAKQLR GFTLTQIFK 
      NPVMSNVLI CCCIINVAN QLSGKNALT YFSVQLLEN 
      LNMASSAVS ITIGMSILS AIVALGIVF IIEYYGRRL 
      LMEISLIGI ILSTFSLFI SLYFRVAFL ENLKYLSVC 
      FVCLFYVFF SIGLGPIPL FFTPELFSN SNRTVATCL 
      ASVSNWAAS FVLGQAFLS LDNEIDFYD CLVFLFLDI 
      FFFFYIRLR MPETAKLSS IQILKILKI YFAILVVSA 
      ASGFQHGYH FVIINNPSK VIQEWITEL KTKKEGVAP 
      TAAGVDLIW AVVVAIFSV GGAVGAFII GIFADNFGR 
      KGSLKWNTC LVLVAAAIT LYSRRAKSY KVLLFGRFL 
      SGITSGLTS GLCQMYSVE ISPDEIRGG LSGVYQVAI 
      SFSMLVAAI AGIVFSEKE TWHYIFIIP IVPAVLQLI 
      ALPFCPESP KFLLITKGS DAKAEKALK WLRESDDVA 
      EELELLKEE DNRVKQLRG SSLKRILSN PAMTNALIC 
      CLILNISQQ MCGIKTVIQ FSTPVFEEM NLGPKVALY 
      ITAGMFIPL LITSIVFCF IIDKYGRRR MAEISLIGL 
      IITTTFLFT SLYLRRKIV PSMKYAAIV GYFVFNGFY 
      CIGIGPLGW FVPAEMFYH SSRTVAVCL SVASNYISS 
      FIIGQAFFI LKSEIDAYT SLIFIVLDI FSLLYIQQR 
      LPETNQLSA LDILDMPPS NKLFVTFDM VRVKTHLQR 
      VFSVFCSKF IFAVIVASL SGFQHGYHL GIVNIPAEV 
      LQEWIREVA INRKGEPAT EGFIMVFWS TLVAIFSLG 
      GIVGGSLTN LFAEKFGRR NSLLWSNVL ALLGIVFLV 
      YSKVSECYE LLVVGRFVA GVNAGLNSG LCQIYLIEI 
      APDKIRGFV GGFYQLIIA MSILISQIM GAVLGDDEQ 
      WPFMFLVGF IPVCLQLCL LPLCPESPK YLFITKGDK 
      EGAEAALVW FRGTNNVYK EMYKLKEEN NLHKSVDDV 
      NLGKLLCDI SLRSRLAVS LIINIGQQL CGLNALIYF 
      SNEIFKNMG MSEELATQT TIAMGAVNV VVTLLGIIF 
      VDCIGRRIV LLVGFLGMG INTLLLGII LNLGKIASF 
      IDYIATVLV FVFVILFAM GVGTIPWFL TGEIFNHTP 
      RSIAVTITV SSNWLFNFV VAQTFLPLQ SVIGNYAFL 
      VFVVFDCFF YFFTVLHLP ETARRSGFV YGFHTGFLN 
      ILSATLMQW YDDVSDERL DTKQIKEPL NLGAIHAWV 
      TISFSIGGV IGAALIWPF ATYLGRKLS LFCSNLIVV 
      FALIIVSFV SPSSTFELL IFGRVLLGV SAGINSGIG 
      PMYLVEISP HCLRGFAGS CYYLIVALS IFMAYVFTH 
      PTIMGEIET WSYVFVVSG CPIIIQSIM LIFCPESPK 
      YLMFDKGDE DRAEDAVAD IEGEEIDEE MDDLREEMA 
      LLDEIGPVT IERLFKDET LRNPLIISA VVMLGQQMT 
      GVTCLLYFS CVIFEHLGF DDFKANAMA LGLAFVNWF 
      CALVCVFAV DKVGRKPLL LASYFGMVL TVFCFTLTM 
      ILMSISVRP NQAIDTIAV VLIYLYVMF FALGAGTIG 
      WFFTAEISN HFARPITVS MTVMLYWIV QLIFVGCFL 
      PFWFMIHTY TFLFYIIFD IATVAFLLL TLRETAMVP 
      SVDITEMFY D
    CDS sequence
    • >TC033126 RA
      atgattggc atcttcgcc gatagcctg gggcgaaag 
      caaagcctc cagtacaac aattccatg gttttcgcc 
      gctgccgcg gttatgttt ttttcaaaa aaggccaag 
      tcgtacatt atgctaatc atgggccga tttatagcc 
      ggaatcaac tccgggcta aacatgggt ctgtgcccc 
      atatacctc atcgaaatt gccccggat cgaatccgg 
      ggagccatc ggtagtctc taccaagtt gttattact 
      tgttccatt gtagtgtcg caaattgcc ggcgttctc 
      ctcggaagt gaagagagc tggccatac ctgttccta 
      ctaccgatc attccagcc ttgttccaa ctcgcgtta 
      ctgccattc tgccccgaa tcgccaaaa tttttaatc 
      acccggttg aaagatagg aaagctcac agagcactg 
      atgctgttg cggagccaa caggacgtc gtggacgag 
      ttcgaacag atgaaggaa gaggatagt ctggcgaaa 
      caactgcgt ggtttcact ctgactcag attttcaaa 
      aatccggtc atgtccaac gttttgatt tgctgttgt 
      attataaat gtggccaac caactgtct ggcaaaaat 
      gccctcacg tatttttct gttcaacta ctggaaaat 
      ctcaacatg gcctcaagt gcggtgtct ataactatt 
      ggaatgagt attttgtcc gcgattgtc gcactaggg 
      attgttttc attattgaa tattacggc cggcggcta 
      ctcatggag attagtctg attggaata attctgtca 
      acattttct ctcttcatt tctctttat tttagggtc 
      gctttcctg gagaacctg aagtacctg tctgtttgt 
      ttcgtgtgt ttattttat gtatttttt tcaatcgga 
      cttggtccg attcccttg ttcttcaca cccgagttg 
      ttctccaat tcaaaccgc accgtcgcc acgtgtttg 
      gcatcggtg agcaactgg gcagcgagt ttcgtcctg 
      ggacaagcg ttcctaagc ctcgataac gaaatcgac 
      ttttacgac tgcttggtt ttcctgttc ttggacatt 
      tttttcttc ttctacatc cgcctccgc atgcccgaa 
      accgcgaaa ctgagttca atccaaata ctcaaaatt 
      ctgaaaatc tacttcgcg atcctcgtc gttagtgcg 
      gcttcgggc ttccagcac ggctatcac ttcgttatt 
      attaacaac ccgtcaaag gtgatacag gaatggatc 
      acggaactg aaaacgaaa aaggaaggg gtagctccg 
      actgcagcc ggagtcgac ctgatctgg gccgttgtc 
      gtggcaatt ttctcggtt gggggagca gttggcgcg 
      tttataatc gggatcttc gcggataac ttcggtcgg 
      aagggtagt ctcaagtgg aacacttgt ctggtcctg 
      gtggcagcg gccataaca ctctattcc aggagagca 
      aagtcgtat aaagtgctt ctcttcggc cgcttcctg 
      tccggaatc acctcgggg ttaacctcc ggtttgtgc 
      caaatgtac tcggttgag atttcgccg gatgagatc 
      cgcggcggg ctctctggg gtctaccaa gtcgcgatt 
      agcttttcg atgcttgtc gctgcgatc gcgggaatt 
      gtcttctcg gagaaagaa acgtggcac tatattttc 
      ataataccc atagtaccg gcagttttg caacttatt 
      gctctaccg ttctgcccg gaatcgccc aaattcctc 
      ttgatcact aaaggcagc gatgccaaa gccgagaag 
      gcgctcaaa tggctgcga gagagcgac gatgttgcg 
      gaagagtta gagttgttg aaagaggag gataatcgg 
      gtcaagcaa ttacgggga tcctctctg aaacgtatt 
      ctgagcaac cccgccatg accaacgct ttgatttgc 
      tgtttaatt ttgaatatt tcgcagcaa atgtgtggc 
      attaaaacg gtaattcaa ttctcgacc ccggttttt 
      gaagaaatg aatctggga ccaaaagtg gccttgtat 
      atcacggca ggaatgttc atacctctg ctaattaca 
      tcgatagtt ttctgtttt attatcgat aaatacgga 
      cgacgtaga atggcagaa attagctta ataggcctg 
      attataaca acaactttc ctttttact tctctgtac 
      ttgagaaga aaaattgta ccaagcatg aagtatgcg 
      gctattgta ggatacttc gtattcaac gggttttat 
      tgcatcggt attggcccc ttgggatgg tttgtccca 
      gctgagatg ttctaccat tcatcccgg actgtcgca 
      gtttgttta tcagttgca tcaaactac atttcaagt 
      tttataata gggcaagcg tttttcatt ttaaagagc 
      gaaatcgat gcatacacc agcctcata tttattgtt 
      ttggacata ttctcgctt ctttacatc cagcaacgc 
      ttacccgag acgaaccaa cttagtgca ttagacatc 
      ctcgatatg cctcccagt aataaatta tttgtcaca 
      tttgacatg gtgcgtgta aaaacgcac ctccaaaga 
      gttttctca gtattttgc tccaaattc attttcgcg 
      gttatcgtc gccagtctt tcaggcttc cagcatggc 
      taccacctt ggcattgtc aacattccg gctgaagtc 
      ttgcaggag tggatccgg gaagtggca attaaccgc 
      aagggagaa cccgcaact gaaggtttc atcatggtg 
      ttttggtca actctcgtt gccattttt tcacttggg 
      ggcattgtc gggggcagt ttgacgaac ctcttcgct 
      gagaaattc ggccgaagg aatagcttg ttgtggagc 
      aatgtttta gctctttta gggattgtt ttccttgtc 
      tattccaaa gtgtccgaa tgttacgaa ttgctcgtt 
      gttgggagg tttgtcgcg ggagtgaac gccgggtta 
      aactcgggg ctttgccaa atctatttg atcgaaatc 
      gctcccgat aaaatccgg gggtttgtt ggggggttc 
      taccagtta attatcgca atgtctatt ttgatttcg 
      caaataatg ggagcggtt ctgggagac gatgagcaa 
      tggccgttt atgtttctg gtcggtttt ataccagtt 
      tgcctacag ttgtgcctt ttaccgcta tgtcccgag 
      tcccctaag tatcttttc ataacgaaa ggcgacaag 
      gagggagcc gaagctgcc ctggtttgg ttcagaggc 
      actaacaac gtttacaaa gaaatgtac aaactcaag 
      gaagaaaac aatttgcac aaaagtgta gacgatgtg 
      aatttgggt aaattactg tgtgatatt tccttgagg 
      agccgtttg gctgtctct ttgattata aatattgga 
      caacagctg tgcggactc aacgctctg atttatttc 
      tcgaacgag atattcaag aatatgggc atgtcggaa 
      gaactggcc acccaaacg accattgcc atgggtgcg 
      gtgaatgtt gtggtaacc ctgttgggt attatcttc 
      gtggattgc atcggtcgt cgcattgtc ttacttgtc 
      ggtttcctg gggatgggt ataaacacg ttacttttg 
      ggcataatc ctaaattta ggaaaaatt gcaagtttt 
      atcgactat atcgcaaca gtattggtt tttgttttc 
      gtaattctg ttcgcgatg ggcgttggt acaatacca 
      tggttcttg actggtgaa attttcaac catacccct 
      cgctcgatt gcggtgacc ataaccgtc agcagtaac 
      tggttgttt aatttcgtg gtggcccaa acctttttg 
      cccttacag agcgtcatt gggaattac gcgtttcta 
      gttttcgtc gtctttgac tgtttcttt tacttcttt 
      actgttctg cacctcccg gaaacagcg cgtcgttcg 
      ggttttgtt tacggcttc cacacaggt ttcctcaat 
      attctctca gcgacactg atgcaatgg tatgacgac 
      gtttcagac gagcggctc gatacaaag caaattaag 
      gagccctta aacctggga gctattcac gcttgggtc 
      acaatttcc ttttcgatt ggaggcgtg atcggagct 
      gcccttata tggcccttc gccacttac ctcggccga 
      aaactatct cttttctgc agtaactta atagtggtg 
      tttgcgctt atcatcgtg agttttgtc agtccttca 
      agcactttc gaattgctg attttcgga agagtcctt 
      ctgggtgtg tcggccgga attaacagt ggaatagga 
      ccaatgtac ttggtcgaa atctcgcct cactgcctg 
      cgaggcttt gccgggagt tgttattac ctgattgtg 
      gctttatca attttcatg gcttatgtt ttcacccat 
      cctacgata atgggggaa atcgagacg tggtcttat 
      gtttttgtc gtgtcggga tgcccgatt ataattcag 
      tctataatg cttatattt tgtcccgaa agccccaaa 
      tacctcatg ttcgataaa ggggatgag gatagagcc 
      gaggacgcg gtagcggac attgaaggg gaagaaatc 
      gacgaagaa atggacgat ttgagggaa gaaatggcc 
      cttttggac gaaattgga ccagtgaca atagaacgg 
      ctgttcaaa gatgagact ttgaggaac cctttaata 
      atctcggct gtcgtcatg ttgggacaa caaatgaca 
      ggagttacc tgtttgttg tacttttcg tgcgtaatt 
      ttcgaacac ctgggcttc gacgatttc aaagccaac 
      gcaatggct ctaggtcta gctttcgtg aactggttt 
      tgcgccctt gtgtgcgta tttgcggtg gacaaagtg 
      ggccgcaag cctctattg ctggctagt tatttcggt 
      atggtgcta acagtgttt tgttttact ctcacgatg 
      attctaatg tcaatttca gtgcgacct aatcaagcc 
      attgacaca atcgccgtc gttctgatt tatctatat 
      gtgatgttt tttgcgctt ggcgctggt accattggg 
      tggttcttt acggcagaa atttcgaac catttcgcc 
      agacccatc accgtttct atgacggtg atgttgtac 
      tggatcgtc caacttatt tttgttggg tgcttttta 
      cctttttgg ttcatgatt cacacttat acgtttttg 
      ttttacatt atattcgat attgcgaca gttgctttt 
      ctcttacta acgctaagg gaaaccgca atggtgcct 
      agtgtcgat atcacggaa atgttttat gattaa
    mRNA sequence
    • >TC033126 RA
      tggattacc cacatcaaa atcgaggaa gaaaaagcg 
      gaagagcca aaacggaga gaactctcc ttgctttgg 
      tccacgaca gtggcgagt tactcaatc ggaggcata 
      atcgggtcc ttcatgatt ggcatcttc gccgatagc 
      ctggggcga aagcaaagc ctccagtac aacaattcc 
      atggttttc gccgctgcc gcggttatg tttttttca 
      aaaaaggcc aagtcgtac attatgcta atcatgggc 
      cgatttata gccggaatc aactccggg ctaaacatg 
      ggtctgtgc cccatatac ctcatcgaa attgccccg 
      gatcgaatc cggggagcc atcggtagt ctctaccaa 
      gttgttatt acttgttcc attgtagtg tcgcaaatt 
      gccggcgtt ctcctcgga agtgaagag agctggcca 
      tacctgttc ctactaccg atcattcca gccttgttc 
      caactcgcg ttactgcca ttctgcccc gaatcgcca 
      aaattttta atcacccgg ttgaaagat aggaaagct 
      cacagagca ctgatgctg ttgcggagc caacaggac 
      gtcgtggac gagttcgaa cagatgaag gaagaggat 
      agtctggcg aaacaactg cgtggtttc actctgact 
      cagattttc aaaaatccg gtcatgtcc aacgttttg 
      atttgctgt tgtattata aatgtggcc aaccaactg 
      tctggcaaa aatgccctc acgtatttt tctgttcaa 
      ctactggaa aatctcaac atggcctca agtgcggtg 
      tctataact attggaatg agtattttg tccgcgatt 
      gtcgcacta gggattgtt ttcattatt gaatattac 
      ggccggcgg ctactcatg gagattagt ctgattgga 
      ataattctg tcaacattt tctctcttc atttctctt 
      tattttagg gtcgctttc ctggagaac ctgaagtac 
      ctgtctgtt tgtttcgtg tgtttattt tatgtattt 
      ttttcaatc ggacttggt ccgattccc ttgttcttc 
      acacccgag ttgttctcc aattcaaac cgcaccgtc 
      gccacgtgt ttggcatcg gtgagcaac tgggcagcg 
      agtttcgtc ctgggacaa gcgttccta agcctcgat 
      aacgaaatc gacttttac gactgcttg gttttcctg 
      ttcttggac atttttttc ttcttctac atccgcctc 
      cgcatgccc gaaaccgcg aaactgagt tcaatccaa 
      atactcaaa attctgaaa atctacttc gcgatcctc 
      gtcgttagt gcggcttcg ggcttccag cacggctat 
      cacttcgtt attattaac aacccgtca aaggtgata 
      caggaatgg atcacggaa ctgaaaacg aaaaaggaa 
      ggggtagct ccgactgca gccggagtc gacctgatc 
      tgggccgtt gtcgtggca attttctcg gttggggga 
      gcagttggc gcgtttata atcgggatc ttcgcggat 
      aacttcggt cggaagggt agtctcaag tggaacact 
      tgtctggtc ctggtggca gcggccata acactctat 
      tccaggaga gcaaagtcg tataaagtg cttctcttc 
      ggccgcttc ctgtccgga atcacctcg gggttaacc 
      tccggtttg tgccaaatg tactcggtt gagatttcg 
      ccggatgag atccgcggc gggctctct ggggtctac 
      caagtcgcg attagcttt tcgatgctt gtcgctgcg 
      atcgcggga attgtcttc tcggagaaa gaaacgtgg 
      cactatatt ttcataata cccatagta ccggcagtt 
      ttgcaactt attgctcta ccgttctgc ccggaatcg 
      cccaaattc ctcttgatc actaaaggc agcgatgcc 
      aaagccgag aaggcgctc aaatggctg cgagagagc 
      gacgatgtt gcggaagag ttagagttg ttgaaagag 
      gaggataat cgggtcaag caattacgg ggatcctct 
      ctgaaacgt attctgagc aaccccgcc atgaccaac 
      gctttgatt tgctgttta attttgaat atttcgcag 
      caaatgtgt ggcattaaa acggtaatt caattctcg 
      accccggtt tttgaagaa atgaatctg ggaccaaaa 
      gtggccttg tatatcacg gcaggaatg ttcatacct 
      ctgctaatt acatcgata gttttctgt tttattatc 
      gataaatac ggacgacgt agaatggca gaaattagc 
      ttaataggc ctgattata acaacaact ttccttttt 
      acttctctg tacttgaga agaaaaatt gtaccaagc 
      atgaagtat gcggctatt gtaggatac ttcgtattc 
      aacgggttt tattgcatc ggtattggc cccttggga 
      tggtttgtc ccagctgag atgttctac cattcatcc 
      cggactgtc gcagtttgt ttatcagtt gcatcaaac 
      tacatttca agttttata atagggcaa gcgtttttc 
      attttaaag agcgaaatc gatgcatac accagcctc 
      atatttatt gttttggac atattctcg cttctttac 
      atccagcaa cgcttaccc gagacgaac caacttagt 
      gcattagac atcctcgat atgcctccc agtaataaa 
      ttatttgtc acatttgac atggtgcgt gtaaaaacg 
      cacctccaa agagttttc tcagtattt tgctccaaa 
      ttcattttc gcggttatc gtcgccagt ctttcaggc 
      ttccagcat ggctaccac cttggcatt gtcaacatt 
      ccggctgaa gtcttgcag gagtggatc cgggaagtg 
      gcaattaac cgcaaggga gaacccgca actgaaggt 
      ttcatcatg gtgttttgg tcaactctc gttgccatt 
      ttttcactt gggggcatt gtcgggggc agtttgacg 
      aacctcttc gctgagaaa ttcggccga aggaatagc 
      ttgttgtgg agcaatgtt ttagctctt ttagggatt 
      gttttcctt gtctattcc aaagtgtcc gaatgttac 
      gaattgctc gttgttggg aggtttgtc gcgggagtg 
      aacgccggg ttaaactcg gggctttgc caaatctat 
      ttgatcgaa atcgctccc gataaaatc cgggggttt 
      gttgggggg ttctaccag ttaattatc gcaatgtct 
      attttgatt tcgcaaata atgggagcg gttctggga 
      gacgatgag caatggccg tttatgttt ctggtcggt 
      tttatacca gtttgccta cagttgtgc cttttaccg 
      ctatgtccc gagtcccct aagtatctt ttcataacg 
      aaaggcgac aaggaggga gccgaagct gccctggtt 
      tggttcaga ggcactaac aacgtttac aaagaaatg 
      tacaaactc aaggaagaa aacaatttg cacaaaagt 
      gtagacgat gtgaatttg ggtaaatta ctgtgtgat 
      atttccttg aggagccgt ttggctgtc tctttgatt 
      ataaatatt ggacaacag ctgtgcgga ctcaacgct 
      ctgatttat ttctcgaac gagatattc aagaatatg 
      ggcatgtcg gaagaactg gccacccaa acgaccatt 
      gccatgggt gcggtgaat gttgtggta accctgttg 
      ggtattatc ttcgtggat tgcatcggt cgtcgcatt 
      gtcttactt gtcggtttc ctggggatg ggtataaac 
      acgttactt ttgggcata atcctaaat ttaggaaaa 
      attgcaagt tttatcgac tatatcgca acagtattg 
      gtttttgtt ttcgtaatt ctgttcgcg atgggcgtt 
      ggtacaata ccatggttc ttgactggt gaaattttc 
      aaccatacc cctcgctcg attgcggtg accataacc 
      gtcagcagt aactggttg tttaatttc gtggtggcc 
      caaaccttt ttgccctta cagagcgtc attgggaat 
      tacgcgttt ctagttttc gtcgtcttt gactgtttc 
      ttttacttc tttactgtt ctgcacctc ccggaaaca 
      gcgcgtcgt tcgggtttt gtttacggc ttccacaca 
      ggtttcctc aatattctc tcagcgaca ctgatgcaa 
      tggtatgac gacgtttca gacgagcgg ctcgataca 
      aagcaaatt aaggagccc ttaaacctg ggagctatt 
      cacgcttgg gtcacaatt tccttttcg attggaggc 
      gtgatcgga gctgccctt atatggccc ttcgccact 
      tacctcggc cgaaaacta tctcttttc tgcagtaac 
      ttaatagtg gtgtttgcg cttatcatc gtgagtttt 
      gtcagtcct tcaagcact ttcgaattg ctgattttc 
      ggaagagtc cttctgggt gtgtcggcc ggaattaac 
      agtggaata ggaccaatg tacttggtc gaaatctcg 
      cctcactgc ctgcgaggc tttgccggg agttgttat 
      tacctgatt gtggcttta tcaattttc atggcttat 
      gttttcacc catcctacg ataatgggg gaaatcgag 
      acgtggtct tatgttttt gtcgtgtcg ggatgcccg 
      attataatt cagtctata atgcttata ttttgtccc 
      gaaagcccc aaatacctc atgttcgat aaaggggat 
      gaggataga gccgaggac gcggtagcg gacattgaa 
      ggggaagaa atcgacgaa gaaatggac gatttgagg 
      gaagaaatg gcccttttg gacgaaatt ggaccagtg 
      acaatagaa cggctgttc aaagatgag actttgagg 
      aacccttta ataatctcg gctgtcgtc atgttggga 
      caacaaatg acaggagtt acctgtttg ttgtacttt 
      tcgtgcgta attttcgaa cacctgggc ttcgacgat 
      ttcaaagcc aacgcaatg gctctaggt ctagctttc 
      gtgaactgg ttttgcgcc cttgtgtgc gtatttgcg 
      gtggacaaa gtgggccgc aagcctcta ttgctggct 
      agttatttc ggtatggtg ctaacagtg ttttgtttt 
      actctcacg atgattcta atgtcaatt tcagtgcga 
      cctaatcaa gccattgac acaatcgcc gtcgttctg 
      atttatcta tatgtgatg ttttttgcg cttggcgct 
      ggtaccatt gggtggttc tttacggca gaaatttcg 
      aaccatttc gccagaccc atcaccgtt tctatgacg 
      gtgatgttg tactggatc gtccaactt atttttgtt 
      gggtgcttt ttacctttt tggttcatg attcacact 
      tatacgttt ttgttttac attatattc gatattgcg 
      acagttgct tttctctta ctaacgcta agggaaacc 
      gcaatggtg cctagtgtc gatatcacg gaaatgttt 
      tatgattaa acttttcgc cgatgtcac ttttgaaaa 
      taaattttt taaaaaaac
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