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Gene information
General information
TC030076 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1826283..1831385
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC030076
    iBeetle screen iB_02587

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    21 eggs/larvae on slide

    larva appendages partially irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head appendages sometimes fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    head appendages position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 20.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    iBeetle screen iB_10044

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC030076 RA
      MLLCFLLGL VCLSTISAT GSDEEADAY FPASEWSDT 
      FDWKLLKAF STPNSRNVL ISPIGLKVV LALLYEGSG 
      GLTEKEFQN VLQFPIEKS KMREQFKNI SKALQPSER 
      SQYILNLGT RIFLDSRVT PQQNFAAKA LDGYRTDIE 
      PTNYSQPIE ASQTINSWI DKLTNGKVS RLVAPGDLE 
      DAIMIIANA IYFKGTWRH QFPKNNTAL GGFYVSPNE 
      ILTVPYMTT TDAFYYFES NELDAKILR LPYKGQNYA 
      LFVVLPNSK AGLSDLMKK VNLHVLKKQ LYMLDKVPV 
      EVRLPKFKF NFQARYMKI LQDFGLTQM FQNTASFPG 
      IARGNIPHL LRMLVVSDI IQKTGIELD EEGSVVYAA 
      THVQVGNKF AEVDYVFNA THPFMFFLE EQTSGTILF 
      VGKVENPLE TVGVPLPPR FGSNQGLES TLSASPNLQ 
      PANPERDAV IRRFNYFDL ELLKEFSES PGNVFISPA 
      SIKTTLALI LEGAKGQSA REIGNALRI DDIEQKEIR 
      EILSRLLFD LKDSTGNTV LESASSIFV SNKYNVVPE 
      FEKKVITYY NGLIKHLDF SNPNLAAKI INSWVSNAT 
      HGLIDQIVG NQHLSPDTA LLLTNALYF KGKWKTEFN 
      PNGTKEKCF FSPTRNCVS TPLMQVTST FNYNFNIDL 
      NAHAIELPY QDDKYAMLI LFPTNGNNV RTLARDMQH 
      AQLHMVIDA LKPTDLILE IPKFEIDYE TDLVSFCDP
    • >TC030076 RB
      MLLCFLLGL VCLSTISAT GSDEEADAY FPASEWSDT 
      FDWKLLKAF STPNSRNVL ISPIGLKVV LALLYEGSG 
      GLTEKEFQN VLQFPIEKS KMREQFKNI SKALQPSER 
      SQYILNLGT RIFLDSRVT PQQNFAAKA LDGYRTDIE 
      PTNYSQPIE ASQTINSWI DKLTNGKVS RLVAPGDLE 
      DAIMIIANA IYFKGTWRH QFPKNNTAL GGFYVSPNE 
      ILTVPYMTT TDAFYYFES NELDAKILR LPYKGQNYA 
      LFVVLPNSK AGLSDLMKK VNLHVLKKQ LYMLDKVPV 
      EVRLPKFKF NFQARYMKI LQDFGLTQM FQNTASFPG 
      IARGNIPHL LRMLVVSDI IQKTGIELD EEGSVVYAA 
      THVQVGNKF AEVDYVFNA THPFMFFLE EQTSGTILF 
      VGKVENPLE TVGVPLPPR FGSNQGKRA KGRGRSACR 
      CGSEMGFAK NHLTVELLL IFLAGLEST LSASPNLQP 
      ANPERDAVI RRFNYFDLE LLKEFSESP GNVFISPAS 
      IKTTLALIL EGAKGQSAR EIGNALRID DIEQKEIRE 
      ILSRLLFDL KDSTGNTVL ESASSIFVS NKYNVVPEF 
      EKKVITYYN GLIKHLDFS NPNLAAKII NSWVSNATH 
      GLIDQIVGN QHLSPDTAL LLTNALYFK GKWKTEFNP 
      NGTKEKCFF SPTRNCVST PLMQVTSTF NYNFNIDLN 
      AHAIELPYQ DDKYAMLIL FPTNGNNVR TLARDMQHA 
      QLHMVIDAL KPTDLILEI PKFEIDYET DLVSFCDP
    CDS sequence
    • >TC030076 RA
      atgttgctg tgttttcta ctgggattg gtgtgccta 
      tcgacgatt tctgccaca ggttccgat gaagaagcc 
      gatgcttat tttcctgct agcgaatgg tccgatact 
      tttgactgg aaactgctc aaggcattt tccaccccc 
      aacagccgc aatgtcttg atatcacct atcgggctg 
      aaagtggtg ctagccctg ctgtatgag ggttccggt 
      ggcctgact gagaaagag tttcaaaac gttttgcaa 
      tttccgatc gaaaagtca aaaatgaga gaacagttt 
      aaaaatatt tccaaagct ctgcagccg agcgaacgg 
      agtcagtac attttgaat ttagggacc cgaattttc 
      ttagattct cgggtaaca ccgcagcag aatttcgct 
      gcgaaggcg ctagatggt tacagaaca gatatagaa 
      ccaacgaat tactcccaa cccattgaa gctagtcaa 
      acgatcaac tcttggatt gataagtta actaatgga 
      aaggtttct cgtttggta gcgccaggt gatctcgaa 
      gacgcgatt atgattatc gctaatgcc atttatttc 
      aagggtact tggcgccat cagttcccc aaaaacaat 
      acagcactt ggtggtttt tacgtatct cctaacgag 
      atactaact gtgccttac atgaccacg actgatgct 
      ttctactac tttgaatca aacgagtta gatgccaaa 
      attctaaga ctgccatac aagggacaa aactacgct 
      ctctttgtc gtactgcca aactccaaa gcaggactt 
      tcggatctt atgaaaaaa gttaactta cacgttctc 
      aagaaacaa ctctacatg ttagacaaa gtgccagtt 
      gaggtgcgg ctgccaaaa ttcaagttc aacttccag 
      gctagatac atgaaaatt ctgcaggac ttcggtttg 
      acccaaatg ttccaaaac actgcaagt ttccctgga 
      atcgctcgg ggaaacatc ccgcatttg ctccgcatg 
      ctggtggtt tcggacatt attcaaaag actgggatt 
      gaacttgac gaagaggga agcgttgtt tatgcagcc 
      acacacgtc caagtgggg aacaaattt gcggaggtt 
      gattatgtg ttcaacgcg acacaccct ttcatgttc 
      ttcttggag gagcagaca agtggcaca attctattc 
      gttggcaaa gtggaaaac cctctggaa accgtaggt 
      gtgccctta ccgcccagg tttggtagt aaccaaggt 
      cttgaatcc actttgtct gctagtcca aatttgcag 
      cccgcaaac ccagaacgg gacgctgta attaggagg 
      tttaattat ttcgactta gaattatta aaagaattt 
      agtgaatcg cctggaaac gtattcata tcacctgcc 
      agtatcaaa acaacattg gctctaatt ttagaaggc 
      gccaaagga cagagcgct cgagaaatc ggaaatgcg 
      ttgagaatt gacgacatt gagcaaaag gaaattcga 
      gagatttta agcagactg ctgtttgat ttgaaagat 
      agcaccggt aatactgtt ttggagtcg gccagttcc 
      atcttcgtt tcaaacaaa tataatgtg gttcctgag 
      tttgagaaa aaagtgatt acgtattac aacggtttg 
      attaaacac ctcgacttt tcaaatccg aatctagcg 
      gcaaaaata atcaattcg tgggtctct aacgccact 
      catggtttg atcgaccaa attgtggga aatcaacat 
      ttgagccct gataccgcg ttgctgcta accaacgcg 
      ttgtacttc aagggaaaa tggaaaact gagtttaat 
      ccaaacggt accaaagaa aagtgtttc ttttcgcca 
      acgcgtaat tgtgtgagc acgcccctg atgcaagtc 
      actagtacc ttcaattac aatttcaac atcgacttg 
      aacgcacat gccatagaa ctgccgtac caggatgac 
      aaatacgcg atgttaatt ctgttccca actaatggt 
      aacaatgtc aggactttg gcacgggat atgcaacat 
      gctcaatta cacatggtt atagacgct ctcaaaccg 
      accgattta atcctcgaa attcccaag ttcgagatc 
      gactacgag accgacttg gtcagtttt tgcgacccc 
      taa
    • >TC030076 RB
      atgttgctg tgttttcta ctgggattg gtgtgccta 
      tcgacgatt tctgccaca ggttccgat gaagaagcc 
      gatgcttat tttcctgct agcgaatgg tccgatact 
      tttgactgg aaactgctc aaggcattt tccaccccc 
      aacagccgc aatgtcttg atatcacct atcgggctg 
      aaagtggtg ctagccctg ctgtatgag ggttccggt 
      ggcctgact gagaaagag tttcaaaac gttttgcaa 
      tttccgatc gaaaagtca aaaatgaga gaacagttt 
      aaaaatatt tccaaagct ctgcagccg agcgaacgg 
      agtcagtac attttgaat ttagggacc cgaattttc 
      ttagattct cgggtaaca ccgcagcag aatttcgct 
      gcgaaggcg ctagatggt tacagaaca gatatagaa 
      ccaacgaat tactcccaa cccattgaa gctagtcaa 
      acgatcaac tcttggatt gataagtta actaatgga 
      aaggtttct cgtttggta gcgccaggt gatctcgaa 
      gacgcgatt atgattatc gctaatgcc atttatttc 
      aagggtact tggcgccat cagttcccc aaaaacaat 
      acagcactt ggtggtttt tacgtatct cctaacgag 
      atactaact gtgccttac atgaccacg actgatgct 
      ttctactac tttgaatca aacgagtta gatgccaaa 
      attctaaga ctgccatac aagggacaa aactacgct 
      ctctttgtc gtactgcca aactccaaa gcaggactt 
      tcggatctt atgaaaaaa gttaactta cacgttctc 
      aagaaacaa ctctacatg ttagacaaa gtgccagtt 
      gaggtgcgg ctgccaaaa ttcaagttc aacttccag 
      gctagatac atgaaaatt ctgcaggac ttcggtttg 
      acccaaatg ttccaaaac actgcaagt ttccctgga 
      atcgctcgg ggaaacatc ccgcatttg ctccgcatg 
      ctggtggtt tcggacatt attcaaaag actgggatt 
      gaacttgac gaagaggga agcgttgtt tatgcagcc 
      acacacgtc caagtgggg aacaaattt gcggaggtt 
      gattatgtg ttcaacgcg acacaccct ttcatgttc 
      ttcttggag gagcagaca agtggcaca attctattc 
      gttggcaaa gtggaaaac cctctggaa accgtaggt 
      gtgccctta ccgcccagg tttggtagt aaccaaggt 
      aagagagct aagggacgt gggaggtct gcttgccgc 
      tgcggctct gaaatgggc ttcgctaaa aaccacctc 
      acggttgag cttttactc atatttctc gcaggtctt 
      gaatccact ttgtctgct agtccaaat ttgcagccc 
      gcaaaccca gaacgggac gctgtaatt aggaggttt 
      aattatttc gacttagaa ttattaaaa gaatttagt 
      gaatcgcct ggaaacgta ttcatatca cctgccagt 
      atcaaaaca acattggct ctaatttta gaaggcgcc 
      aaaggacag agcgctcga gaaatcgga aatgcgttg 
      agaattgac gacattgag caaaaggaa attcgagag 
      attttaagc agactgctg tttgatttg aaagatagc 
      accggtaat actgttttg gagtcggcc agttccatc 
      ttcgtttca aacaaatat aatgtggtt cctgagttt 
      gagaaaaaa gtgattacg tattacaac ggtttgatt 
      aaacacctc gacttttca aatccgaat ctagcggca 
      aaaataatc aattcgtgg gtctctaac gccactcat 
      ggtttgatc gaccaaatt gtgggaaat caacatttg 
      agccctgat accgcgttg ctgctaacc aacgcgttg 
      tacttcaag ggaaaatgg aaaactgag tttaatcca 
      aacggtacc aaagaaaag tgtttcttt tcgccaacg 
      cgtaattgt gtgagcacg cccctgatg caagtcact 
      agtaccttc aattacaat ttcaacatc gacttgaac 
      gcacatgcc atagaactg ccgtaccag gatgacaaa 
      tacgcgatg ttaattctg ttcccaact aatggtaac 
      aatgtcagg actttggca cgggatatg caacatgct 
      caattacac atggttata gacgctctc aaaccgacc 
      gatttaatc ctcgaaatt cccaagttc gagatcgac 
      tacgagacc gacttggtc agtttttgc gacccctaa
    mRNA sequence
    • >TC030076 RA
      gccatcgca aaacccgaa tacagtttt acctgttgt 
      ctctttagc aaactttgt gctttatag ttgttcctc 
      gttccgatt tcattctaa ctgaatcat cggttttgt 
      acacacaac gggaatgtt gcccgcttt gcaattatc 
      tgtaaactg ataaaagac gtattttcc atattttgt 
      ggacgctat ctcaaacat aaacaagta tctgaagac 
      agggtcaac tgctgaagc tgtaacaat ctagtgtag 
      cattgtgac catcaagga aatgttgct gtgttttct 
      actgggatt ggtgtgcct atcgacgat ttctgccac 
      aggttccga tgaagaagc cgatgctta ttttcctgc 
      tagcgaatg gtccgatac ttttgactg gaaactgct 
      caaggcatt ttccacccc caacagccg caatgtctt 
      gatatcacc tatcgggct gaaagtggt gctagccct 
      gctgtatga gggttccgg tggcctgac tgagaaaga 
      gtttcaaaa cgttttgca atttccgat cgaaaagtc 
      aaaaatgag agaacagtt taaaaatat ttccaaagc 
      tctgcagcc gagcgaacg gagtcagta cattttgaa 
      tttagggac ccgaatttt cttagattc tcgggtaac 
      accgcagca gaatttcgc tgcgaaggc gctagatgg 
      ttacagaac agatataga accaacgaa ttactccca 
      acccattga agctagtca aacgatcaa ctcttggat 
      tgataagtt aactaatgg aaaggtttc tcgtttggt 
      agcgccagg tgatctcga agacgcgat tatgattat 
      cgctaatgc catttattt caagggtac ttggcgcca 
      tcagttccc caaaaacaa tacagcact tggtggttt 
      ttacgtatc tcctaacga gatactaac tgtgcctta 
      catgaccac gactgatgc tttctacta ctttgaatc 
      aaacgagtt agatgccaa aattctaag actgccata 
      caagggaca aaactacgc tctctttgt cgtactgcc 
      aaactccaa agcaggact ttcggatct tatgaaaaa 
      agttaactt acacgttct caagaaaca actctacat 
      gttagacaa agtgccagt tgaggtgcg gctgccaaa 
      attcaagtt caacttcca ggctagata catgaaaat 
      tctgcagga cttcggttt gacccaaat gttccaaaa 
      cactgcaag tttccctgg aatcgctcg gggaaacat 
      cccgcattt gctccgcat gctggtggt ttcggacat 
      tattcaaaa gactgggat tgaacttga cgaagaggg 
      aagcgttgt ttatgcagc cacacacgt ccaagtggg 
      gaacaaatt tgcggaggt tgattatgt gttcaacgc 
      gacacaccc tttcatgtt cttcttgga ggagcagac 
      aagtggcac aattctatt cgttggcaa agtggaaaa 
      ccctctgga aaccgtagg tgtgccctt accgcccag 
      gtttggtag taaccaagg tcttgaatc cactttgtc 
      tgctagtcc aaatttgca gcccgcaaa cccagaacg 
      ggacgctgt aattaggag gtttaatta tttcgactt 
      agaattatt aaaagaatt tagtgaatc gcctggaaa 
      cgtattcat atcacctgc cagtatcaa aacaacatt 
      ggctctaat tttagaagg cgccaaagg acagagcgc 
      tcgagaaat cggaaatgc gttgagaat tgacgacat 
      tgagcaaaa ggaaattcg agagatttt aagcagact 
      gctgtttga tttgaaaga tagcaccgg taatactgt 
      tttggagtc ggccagttc catcttcgt ttcaaacaa 
      atataatgt ggttcctga gtttgagaa aaaagtgat 
      tacgtatta caacggttt gattaaaca cctcgactt 
      ttcaaatcc gaatctagc ggcaaaaat aatcaattc 
      gtgggtctc taacgccac tcatggttt gatcgacca 
      aattgtggg aaatcaaca tttgagccc tgataccgc 
      gttgctgct aaccaacgc gttgtactt caagggaaa 
      atggaaaac tgagtttaa tccaaacgg taccaaaga 
      aaagtgttt cttttcgcc aacgcgtaa ttgtgtgag 
      cacgcccct gatgcaagt cactagtac cttcaatta 
      caatttcaa catcgactt gaacgcaca tgccataga 
      actgccgta ccaggatga caaatacgc gatgttaat 
      tctgttccc aactaatgg taacaatgt caggacttt 
      ggcacggga tatgcaaca tgctcaatt acacatggt 
      tatagacgc tctcaaacc gaccgattt aatcctcga 
      aattcccaa gttcgagat cgactacga gaccgactt 
      ggtcagttt ttgcgaccc ctaaaatcc aggaatttt 
      ttccaagca aacgccaac ttgaccgga atggtcgcg 
      caagagaaa gtcaaaatc agcaacata gtgcataaa 
      actaaagtt caggtcaac gaacaaggc actagggcc 
      gccgctgtc accactgct gtggtcatt ccgctaatg 
      ggctcgact gcacaaaga atagtggtg gaccagccg 
      ttcatcttc ttcatttat cacagggaa tcgaggaac 
      attattttc gagggactc ttcgtcgaa cctggcgca 
      ccaaagact gctccaaat accaaccgg tattactcg 
      caagtgcca caaaggagt ggtgtcaac taccaacga 
      cgtgacttt aattatcaa agatcgaac aatcgctac 
      cagtaattc gaagacttt ggcggaaat agtctagtt 
      gcgcaatac tctgaatga gattcaaat tttgttttt 
      atttttaat acacgattc ctaataac
    • >TC030076 RB
      gccatcgca aaacccgaa tacagtttt acctgttgt 
      ctctttagc aaactttgt gctttatag ttgttcctc 
      gttccgatt tcattctaa ctgaatcat cggttttgt 
      acacacaac gggaatgtt gcccgcttt gcaattatc 
      tgtaaactg ataaaagac gtattttcc atattttgt 
      ggacgctat ctcaaacat aaacaagta tctgaagac 
      agggtcaac tgctgaagc tgtaacaat ctagtgtag 
      cattgtgac catcaagga aatgttgct gtgttttct 
      actgggatt ggtgtgcct atcgacgat ttctgccac 
      aggttccga tgaagaagc cgatgctta ttttcctgc 
      tagcgaatg gtccgatac ttttgactg gaaactgct 
      caaggcatt ttccacccc caacagccg caatgtctt 
      gatatcacc tatcgggct gaaagtggt gctagccct 
      gctgtatga gggttccgg tggcctgac tgagaaaga 
      gtttcaaaa cgttttgca atttccgat cgaaaagtc 
      aaaaatgag agaacagtt taaaaatat ttccaaagc 
      tctgcagcc gagcgaacg gagtcagta cattttgaa 
      tttagggac ccgaatttt cttagattc tcgggtaac 
      accgcagca gaatttcgc tgcgaaggc gctagatgg 
      ttacagaac agatataga accaacgaa ttactccca 
      acccattga agctagtca aacgatcaa ctcttggat 
      tgataagtt aactaatgg aaaggtttc tcgtttggt 
      agcgccagg tgatctcga agacgcgat tatgattat 
      cgctaatgc catttattt caagggtac ttggcgcca 
      tcagttccc caaaaacaa tacagcact tggtggttt 
      ttacgtatc tcctaacga gatactaac tgtgcctta 
      catgaccac gactgatgc tttctacta ctttgaatc 
      aaacgagtt agatgccaa aattctaag actgccata 
      caagggaca aaactacgc tctctttgt cgtactgcc 
      aaactccaa agcaggact ttcggatct tatgaaaaa 
      agttaactt acacgttct caagaaaca actctacat 
      gttagacaa agtgccagt tgaggtgcg gctgccaaa 
      attcaagtt caacttcca ggctagata catgaaaat 
      tctgcagga cttcggttt gacccaaat gttccaaaa 
      cactgcaag tttccctgg aatcgctcg gggaaacat 
      cccgcattt gctccgcat gctggtggt ttcggacat 
      tattcaaaa gactgggat tgaacttga cgaagaggg 
      aagcgttgt ttatgcagc cacacacgt ccaagtggg 
      gaacaaatt tgcggaggt tgattatgt gttcaacgc 
      gacacaccc tttcatgtt cttcttgga ggagcagac 
      aagtggcac aattctatt cgttggcaa agtggaaaa 
      ccctctgga aaccgtagg tgtgccctt accgcccag 
      gtttggtag taaccaagg taagagagc taagggacg 
      tgggaggtc tgcttgccg ctgcggctc tgaaatggg 
      cttcgctaa aaaccacct cacggttga gcttttact 
      catatttct cgcaggtct tgaatccac tttgtctgc 
      tagtccaaa tttgcagcc cgcaaaccc agaacggga 
      cgctgtaat taggaggtt taattattt cgacttaga 
      attattaaa agaatttag tgaatcgcc tggaaacgt 
      attcatatc acctgccag tatcaaaac aacattggc 
      tctaatttt agaaggcgc caaaggaca gagcgctcg 
      agaaatcgg aaatgcgtt gagaattga cgacattga 
      gcaaaagga aattcgaga gattttaag cagactgct 
      gtttgattt gaaagatag caccggtaa tactgtttt 
      ggagtcggc cagttccat cttcgtttc aaacaaata 
      taatgtggt tcctgagtt tgagaaaaa agtgattac 
      gtattacaa cggtttgat taaacacct cgacttttc 
      aaatccgaa tctagcggc aaaaataat caattcgtg 
      ggtctctaa cgccactca tggtttgat cgaccaaat 
      tgtgggaaa tcaacattt gagccctga taccgcgtt 
      gctgctaac caacgcgtt gtacttcaa gggaaaatg 
      gaaaactga gtttaatcc aaacggtac caaagaaaa 
      gtgtttctt ttcgccaac gcgtaattg tgtgagcac 
      gcccctgat gcaagtcac tagtacctt caattacaa 
      tttcaacat cgacttgaa cgcacatgc catagaact 
      gccgtacca ggatgacaa atacgcgat gttaattct 
      gttcccaac taatggtaa caatgtcag gactttggc 
      acgggatat gcaacatgc tcaattaca catggttat 
      agacgctct caaaccgac cgatttaat cctcgaaat 
      tcccaagtt cgagatcga ctacgagac cgacttggt 
      cagtttttg cgaccccta aaatccagg aattttttc 
      caagcaaac gccaacttg accggaatg gtcgcgcaa 
      gagaaagtc aaaatcagc aacatagtg cataaaact 
      aaagttcag gtcaacgaa caaggcact agggccgcc 
      gctgtcacc actgctgtg gtcattccg ctaatgggc 
      tcgactgca caaagaata gtggtggac cagccgttc 
      atcttcttc atttatcac agggaatcg aggaacatt 
      attttcgag ggactcttc gtcgaacct ggcgcacca 
      aagactgct ccaaatacc aaccggtat tactcgcaa 
      gtgccacaa aggagtggt gtcaactac caacgacgt 
      gactttaat tatcaaaga tcgaacaat cgctaccag 
      taattcgaa gactttggc ggaaatagt ctagttgcg 
      caatactct gaatgagat tcaaatttt gtttttatt 
      tttaataca cgattccta ataac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY