| General information | |||
| TC014310 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007420.3: 8927091..8932588 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC014310 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_10043 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
vitellogenic egg chamber late deposition irregular - Number of animals showing the phenotype: 4
mature egg not present -
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC014310 RA
MAPKKKRGG QNRVTFAPS KMSTRSGKK TATSPKTSK AKVPKMPKL ATKATAKKG KAVPKAKKA APKKTKIEV TSTDSEPLE MESEIDSKN NDEDLQPEP IEQVFLKMD DNFMETTEP EGEETETCE IREQLESLN VEEAHEPKP GNEEVNEHS SEESVVEEV IEIDGCGEY EYITEEFVP VVENAEVII IDDEVEANN QVQEKPEQI ISSEEKIQE MKLDETPQE EAKVDETKI VETPEQTKV EDKPAEETE IEDKLKEIE IETKLKKTV IEDKLEETK VEDKPEETK IDDKPEETK IDDKPEETK EQPMETVTE EAPEPEPEN KTVPEEVKK VEEPKKRPD APKTKKKVP KPKEPKKNP PKKKAAKPK PKKEPTDKT EEVRRSSRI KSINVLKQR SKGHGLVKS AKLPPDENK ESATTQSSE EKSESATSL PATEADNKP VKVKSRWRR SSELEMNVL TPAAIVMET CGDSEKIAE ANNLENKTK PTHDEEVTT RLKQFVHLK ENLYLTDRM SCKEAKKMT CDCFLTPEE IERGELGCG EDCLNRLLM IECGGLCPV GDRCTNKKF QKSQFAPVE VFKTEKKGL GLRAAANIP YGEFILEYV GEVLDPEEF DNRADDYSN DKNKHYYFM SLRADAIID ATMKGNISR FINHSCDPN AETQKWTVN GELRIGFFS TRTILAGEE ITFDYRFQR YGKEAQKCY CESSLCRGW LGEEPDDED DEDEEDEEE EEAEETKDK VEEDKPEAE KIDSEAKET KSVEKVQKP PVKEKKKRS PRKKPRKEL LEDLDQLDD EIDMLVTTG LKNQAHTLK LSRLMVRAK EPGQRAKLL RVLRRGELP CRRLFLDYH GLRLMHGYM TDAQDLAKT DKKYESLRL EMLQTLATL PIPNKTMLQ DSKVLPTVE IWSSGKVNE LPVDSDSSS PQTDQEPSQ TPKEDDKKK EENVKEEEE VKDVKMDED VEIRKSRGM EFQGSSENV GFTNTHGKE LADEISSIF QEEDNTSSP TPPETKIVK PEVKSDEPN YQEEITALA LKLLEEWSN LKEVFKIPK KERIEQMKE HEREANRGY IAGLGLEQD AEKKNESRY RILKRYRSI DKVDNSARK NIKLEDRTI ANFPKLGKY ERRKMFALQ VEQREEERR RKQREMWRQ HEQHCILLG TDPRLTAPF DPNRGYQCI WNPQIGQWQ NYPIQNPNV IFNPQNPPV VSGMNNPNY PYLHPPGMI PNNVNPLHA LPSLPYQEE VTKEEDYSQ VKFMGPIPP PVKLPPKWK CAKDKYGRP YYYHIKIRK SQWEPPPFS QPQEEDLDD TSETSSSSD TSSLSSDTT SDESDSDDD LDDSKLLMQ VKRQMEKQV KIALPAPVK TEEDEASPV VEDKEEETK LETQVCEEN SEETPQVEE NNDEKAQPG AKKRRTGLV QEIIISPRT EEDRLQFKE DLKRYKLNK EKLKREKEM IVQQTKKHA KEKRKSTIK LKMREMSDS NSETAKKVK EVFRSNMAT VVVSVLNAY RKPDCKEGR ITNTDDFKH LARKLTHFV MLKEMKHIE KIDDLVCTE NVKAKAKEY IRKYMSKFG ENYQKRNDE PDY
- >TC014310 RA
atggcaccg aagaagaag cgtgggggg caaaaccga gtcactttt gcaccgagt aaaatgtca acaaggtct ggtaaaaag accgctaca agtccaaaa accagcaaa gcaaaagtg ccgaaaatg cccaaattg gccacgaag gcgacagct aagaaggga aaagcggtc cctaaagcc aaaaaagct gcaccgaaa aaaaccaaa attgaggtt acgtcaaca gactctgag ccactcgaa atggagtcg gaaattgat agcaaaaac aacgatgag gatttacaa ccagaaccc atcgagcag gttttcctc aaaatggat gataatttc atggaaacg actgaacca gaaggtgaa gaaactgag acttgtgaa attagagaa cagctcgaa agtctcaat gtggaggag gcccacgaa cccaaacca gggaatgag gaggtgaat gagcatagt agtgaagaa agtgtggtt gaagaagta atagagatt gatgggtgt ggagaatat gagtacatc accgaggaa ttcgtccca gttgtggaa aacgccgaa gtgataatt atagacgat gaagtggag gcaaataat caagtgcaa gaaaagccg gaacaaata ataagttcc gaggaaaaa attcaagaa atgaagctt gacgaaaca ccgcaagaa gaggctaaa gttgacgaa acaaaaatt gtggagaca cctgaacaa acaaaagta gaggataaa ccagccgaa gaaacagag attgaagat aaacttaaa gaaatcgag attgagacc aaactgaaa aaaaccgtg attgaggat aaacttgag gaaacaaag gttgaggat aaacctgaa gaaaccaaa atagacgat aagcctgaa gaaaccaag atagacgat aaacctgaa gaaacgaaa gaacaacca atggaaact gtaacagag gaggcacct gaacctgaa cccgaaaat aaaacagtt ccagaggaa gttaagaaa gttgaagag cccaaaaaa cgccctgac gcccccaaa acgaagaag aaggtccct aagcccaaa gaacccaag aagaaccct ccaaaaaag aaagccgcg aagcccaaa ccgaaaaaa gaacccacc gataaaaca gaagaagtg aggcgttcg agtcggatc aagtcaatt aatgtccta aagcagcgc tcaaaaggc cacggttta gtaaaatca gccaaactc ccccctgac gaaaacaaa gaatcagca actacccag tcaagtgaa gaaaagtcc gaatcggcc acttcactc ccagccacc gaagccgac aacaaacca gtcaaagtc aaatctcga tggaggaga tcgagcgaa ttagaaatg aacgttctt acaccggct gcgattgtg atggagact tgcggagac agtgaaaaa attgcagaa gccaataat ttggaaaat aaaacgaaa ccaactcac gacgaagaa gtcaccacg cgattgaaa cagtttgta catttgaag gaaaatctc tatttgact gatcgcatg agctgcaag gaggcgaag aaaatgacc tgtgattgc ttcctgacg ccggaggag attgaaagg ggggaattg ggctgtggg gaagattgc ttaaatcgc ttactaatg atagaatgt gggggttta tgcccagtt ggagacaga tgtaccaat aaaaaattc caaaaatcg cagtttgcc ccagttgag gtttttaaa accgagaag aagggactg gggttgaga gctgcagcc aacattcct tatggtgaa tttattctg gaatatgtc ggcgaagta ttggaccct gaagagttt gataaccgt gccgacgac tattcaaac gataaaaac aaacattac tacttcatg tcgttacga gccgatgcg ataatagac gcaacaatg aaaggaaat atctcacgt tttattaac cattcctgt gacccaaac gctgaaact caaaaatgg actgttaat ggcgagtta cgtatcggt tttttcagc acacgtacg atcttagcg ggtgaagaa atcaccttt gattaccga tttcaacgt tatggaaaa gaggcgcaa aagtgttac tgcgagtcg tcgctttgt cgcggttgg ctgggtgaa gaaccggac gatgaagac gacgaagac gaggaggac gaagaagag gaagaagct gaggaaact aaagacaaa gtcgaggaa gataaacca gaagctgag aaaatcgac agtgaagca aaagaaacc aagagtgtt gaaaaagtg caaaaacct ccagtgaaa gagaagaag aagagatcg ccacggaag aaaccgaga aaagaattg ttggaggac ctagatcaa ctggacgac gaaatagac atgttggtg accacaggg ttgaagaat caagctcac actttaaaa ctgagcaga ttgatggtc agagccaag gagcccggc caacgggcg aagctattg agagtctta agaaggggc gaattacca tgcagacga cttttcttg gactatcat gggttgaga ctcatgcat ggctacatg accgacgcc caagacctg gcaaagact gacaaaaaa tacgaatca ctcagattg gagatgtta caaacgtta gcaacgctg ccaatcccc aacaaaacg atgctgcaa gacagtaaa gtgttacca actgttgaa atatggtcc tctggtaaa gttaacgag ttgccggtg gattctgat agttcttcg cctcaaacc gatcaggag ccaagtcaa acacccaaa gaagatgat aagaaaaaa gaggaaaat gtgaaggag gaagaagaa gtgaaagat gttaaaatg gatgaggat gtggaaata cgcaagtcc agaggaatg gaattccag ggtagctcg gaaaatgtg ggttttacg aacacacat gggaaggaa ttagccgac gaaatttcg agcattttc caagaagaa gacaatact tcgtcaccc acaccaccg gagaccaaa atcgtgaaa cccgaggtg aaatctgac gagcctaat taccaggag gagatcaca gctttggcc ttaaaactg ttggaagag tggtccaac ttgaaggaa gtcttcaaa attcccaaa aaagaacga atcgaacaa atgaaagag cacgaacgt gaggccaac cgtggctac atcgccggt ttggggttg gaacaagac gccgaaaag aaaaacgaa agtcgctat cggatttta aaacggtac agatccatt gataaagtc gataacagc gcgcgaaaa aacatcaaa ctggaagac cggacgata gccaacttc cccaaattg ggcaaatac gagcggagg aaaatgttt gcgttgcaa gtggagcaa agggaggag gagaggcgg cgaaaacag agggagatg tggaggcag catgagcaa cactgtatt ctcctcggg acggatccc cgcctgaca gccccattt gaccccaac agggggtac cagtgcata tggaacccc caaatcggg caatggcaa aactacccc atccaaaat cccaatgtt attttcaac ccccaaaat ccacccgtt gtctcaggg atgaacaac ccgaattat ccctacttg caccctccg ggaatgatc ccgaacaac gtgaacccg ctgcacgca ctacccagt cttccatac caggaggaa gtgaccaaa gaggaggac tactcacaa gttaagttc atgggaccc attccgccc ccagtcaaa ctaccaccg aaatggaaa tgtgccaaa gacaagtac ggacggcct tattattac cacatcaag atcaggaaa agtcagtgg gaaccgcca ccgttttcg caaccacag gaggaagac ttggatgac acgagcgaa acgtcttca agctctgac acgagtagc ttgagctcg gacacgacc tcggacgag tcagacagt gacgatgat ttggacgac tccaaactg ttgatgcag gtgaaaaga cagatggaa aaacaagta aaaattgca ttacccgcc cctgttaaa accgaagaa gatgaagct agtcccgtg gtcgaggac aaggaagaa gagactaaa cttgaaact caagtttgt gaggaaaat agcgaagaa acacctcag gttgaggaa aacaatgac gagaaagcg caaccaggg gctaagaag cgacgaact ggactggtc caggaaatt atcattagt ccgaggact gaagaggac cgattgcaa ttcaaggaa gacttgaag cgttacaaa ctaaataaa gaaaagttg aagcgggag aaggaaatg atcgtccag caaacaaaa aaacacgcg aaggagaag cgcaaaagt actattaaa ttgaaaatg agggaaatg agtgattcc aacagcgaa actgccaag aaagttaag gaggttttc cgttcgaat atggcgact gttgtcgtc agtgttctt aacgcgtat agaaagccg gattgtaaa gaagggaga attacgaat acagatgat tttaagcat ttagctaga aaattgact cactttgtt atgttgaaa gaaatgaaa catattgag aagattgat gatttagtc tgcactgaa aacgtgaaa gcgaaagct aaggaatac atccgcaaa tatatgagt aagttcgga gaaaattat cagaagcga aatgatgag cccgattat taa
- >TC014310 RA
gtggccgag ctccaactg tgcaaacat ccctaaatg ttgtgagtg gttgtgttg ttaagaaat catgttttt acgtgttta ggagcaaat gttggttat tttaatcga aaacaagtg ggatggtca gccatttgg cccattgag agtttgcta gtgtgtcag ttatgcacg ggaataatt aggcgattt atgtgcaag acagtaaaa tcggctcta ctaacgcaa agttgattc atcatggca ccgaagaag aagcgtggg gggcaaaac cgagtcact tttgcaccg agtaaaatg tcaacaagg tctggtaaa aagaccgct acaagtcca aaaaccagc aaagcaaaa gtgccgaaa atgcccaaa ttggccacg aaggcgaca gctaagaag ggaaaagcg gtccctaaa gccaaaaaa gctgcaccg aaaaaaacc aaaattgag gttacgtca acagactct gagccactc gaaatggag tcggaaatt gatagcaaa aacaacgat gaggattta caaccagaa cccatcgag caggttttc ctcaaaatg gatgataat ttcatggaa acgactgaa ccagaaggt gaagaaact gagacttgt gaaattaga gaacagctc gaaagtctc aatgtggag gaggcccac gaacccaaa ccagggaat gaggaggtg aatgagcat agtagtgaa gaaagtgtg gttgaagaa gtaatagag attgatggg tgtggagaa tatgagtac atcaccgag gaattcgtc ccagttgtg gaaaacgcc gaagtgata attatagac gatgaagtg gaggcaaat aatcaagtg caagaaaag ccggaacaa ataataagt tccgaggaa aaaattcaa gaaatgaag cttgacgaa acaccgcaa gaagaggct aaagttgac gaaacaaaa attgtggag acacctgaa caaacaaaa gtagaggat aaaccagcc gaagaaaca gagattgaa gataaactt aaagaaatc gagattgag accaaactg aaaaaaacc gtgattgag gataaactt gaggaaaca aaggttgag gataaacct gaagaaacc aaaatagac gataagcct gaagaaacc aagatagac gataaacct gaagaaacg aaagaacaa ccaatggaa actgtaaca gaggaggca cctgaacct gaacccgaa aataaaaca gttccagag gaagttaag aaagttgaa gagcccaaa aaacgccct gacgccccc aaaacgaag aagaaggtc cctaagccc aaagaaccc aagaagaac cctccaaaa aagaaagcc gcgaagccc aaaccgaaa aaagaaccc accgataaa acagaagaa gtgaggcgt tcgagtcgg atcaagtca attaatgtc ctaaagcag cgctcaaaa ggccacggt ttagtaaaa tcagccaaa ctcccccct gacgaaaac aaagaatca gcaactacc cagtcaagt gaagaaaag tccgaatcg gccacttca ctcccagcc accgaagcc gacaacaaa ccagtcaaa gtcaaatct cgatggagg agatcgagc gaattagaa atgaacgtt cttacaccg gctgcgatt gtgatggag acttgcgga gacagtgaa aaaattgca gaagccaat aatttggaa aataaaacg aaaccaact cacgacgaa gaagtcacc acgcgattg aaacagttt gtacatttg aaggaaaat ctctatttg actgatcgc atgagctgc aaggaggcg aagaaaatg acctgtgat tgcttcctg acgccggag gagattgaa aggggggaa ttgggctgt ggggaagat tgcttaaat cgcttacta atgatagaa tgtgggggt ttatgccca gttggagac agatgtacc aataaaaaa ttccaaaaa tcgcagttt gccccagtt gaggttttt aaaaccgag aagaaggga ctggggttg agagctgca gccaacatt ccttatggt gaatttatt ctggaatat gtcggcgaa gtattggac cctgaagag tttgataac cgtgccgac gactattca aacgataaa aacaaacat tactacttc atgtcgtta cgagccgat gcgataata gacgcaaca atgaaagga aatatctca cgttttatt aaccattcc tgtgaccca aacgctgaa actcaaaaa tggactgtt aatggcgag ttacgtatc ggttttttc agcacacgt acgatctta gcgggtgaa gaaatcacc tttgattac cgatttcaa cgttatgga aaagaggcg caaaagtgt tactgcgag tcgtcgctt tgtcgcggt tggctgggt gaagaaccg gacgatgaa gacgacgaa gacgaggag gacgaagaa gaggaagaa gctgaggaa actaaagac aaagtcgag gaagataaa ccagaagct gagaaaatc gacagtgaa gcaaaagaa accaagagt gttgaaaaa gtgcaaaaa cctccagtg aaagagaag aagaagaga tcgccacgg aagaaaccg agaaaagaa ttgttggag gacctagat caactggac gacgaaata gacatgttg gtgaccaca gggttgaag aatcaagct cacacttta aaactgagc agattgatg gtcagagcc aaggagccc ggccaacgg gcgaagcta ttgagagtc ttaagaagg ggcgaatta ccatgcaga cgacttttc ttggactat catgggttg agactcatg catggctac atgaccgac gcccaagac ctggcaaag actgacaaa aaatacgaa tcactcaga ttggagatg ttacaaacg ttagcaacg ctgccaatc cccaacaaa acgatgctg caagacagt aaagtgtta ccaactgtt gaaatatgg tcctctggt aaagttaac gagttgccg gtggattct gatagttct tcgcctcaa accgatcag gagccaagt caaacaccc aaagaagat gataagaaa aaagaggaa aatgtgaag gaggaagaa gaagtgaaa gatgttaaa atggatgag gatgtggaa atacgcaag tccagagga atggaattc cagggtagc tcggaaaat gtgggtttt acgaacaca catgggaag gaattagcc gacgaaatt tcgagcatt ttccaagaa gaagacaat acttcgtca cccacacca ccggagacc aaaatcgtg aaacccgag gtgaaatct gacgagcct aattaccag gaggagatc acagctttg gccttaaaa ctgttggaa gagtggtcc aacttgaag gaagtcttc aaaattccc aaaaaagaa cgaatcgaa caaatgaaa gagcacgaa cgtgaggcc aaccgtggc tacatcgcc ggtttgggg ttggaacaa gacgccgaa aagaaaaac gaaagtcgc tatcggatt ttaaaacgg tacagatcc attgataaa gtcgataac agcgcgcga aaaaacatc aaactggaa gaccggacg atagccaac ttccccaaa ttgggcaaa tacgagcgg aggaaaatg tttgcgttg caagtggag caaagggag gaggagagg cggcgaaaa cagagggag atgtggagg cagcatgag caacactgt attctcctc gggacggat ccccgcctg acagcccca tttgacccc aacaggggg taccagtgc atatggaac ccccaaatc gggcaatgg caaaactac cccatccaa aatcccaat gttattttc aacccccaa aatccaccc gttgtctca gggatgaac aacccgaat tatccctac ttgcaccct ccgggaatg atcccgaac aacgtgaac ccgctgcac gcactaccc agtcttcca taccaggag gaagtgacc aaagaggag gactactca caagttaag ttcatggga cccattccg cccccagtc aaactacca ccgaaatgg aaatgtgcc aaagacaag tacggacgg ccttattat taccacatc aagatcagg aaaagtcag tgggaaccg ccaccgttt tcgcaacca caggaggaa gacttggat gacacgagc gaaacgtct tcaagctct gacacgagt agcttgagc tcggacacg acctcggac gagtcagac agtgacgat gatttggac gactccaaa ctgttgatg caggtgaaa agacagatg gaaaaacaa gtaaaaatt gcattaccc gcccctgtt aaaaccgaa gaagatgaa gctagtccc gtggtcgag gacaaggaa gaagagact aaacttgaa actcaagtt tgtgaggaa aatagcgaa gaaacacct caggttgag gaaaacaat gacgagaaa gcgcaacca ggggctaag aagcgacga actggactg gtccaggaa attatcatt agtccgagg actgaagag gaccgattg caattcaag gaagacttg aagcgttac aaactaaat aaagaaaag ttgaagcgg gagaaggaa atgatcgtc cagcaaaca aaaaaacac gcgaaggag aagcgcaaa agtactatt aaattgaaa atgagggaa atgagtgat tccaacagc gaaactgcc aagaaagtt aaggaggtt ttccgttcg aatatggcg actgttgtc gtcagtgtt cttaacgcg tatagaaag ccggattgt aaagaaggg agaattacg aatacagat gattttaag catttagct agaaaattg actcacttt gttatgttg aaagaaatg aaacatatt gagaagatt gatgattta gtctgcact gaaaacgtg aaagcgaaa gctaaggaa tacatccgc aaatatatg agtaagttc ggagaaaat tatcagaag cgaaatgat gagcccgat tattaattt cattcattt tttatttgt ctgtgataa aaatcattt catttttca acactgtac gagtcgata agtattgta aaaatgtat gaataattt ataataaaa taggtgaat acgt
