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Gene information
General information
TC014310 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8927091..8932588
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014310
    iBeetle screen iB_10043

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (9 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late number increased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late deposition irregular - Number of animals showing the phenotype: 4
    mature egg not present -
    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014310 RA
      MAPKKKRGG QNRVTFAPS KMSTRSGKK TATSPKTSK 
      AKVPKMPKL ATKATAKKG KAVPKAKKA APKKTKIEV 
      TSTDSEPLE MESEIDSKN NDEDLQPEP IEQVFLKMD 
      DNFMETTEP EGEETETCE IREQLESLN VEEAHEPKP 
      GNEEVNEHS SEESVVEEV IEIDGCGEY EYITEEFVP 
      VVENAEVII IDDEVEANN QVQEKPEQI ISSEEKIQE 
      MKLDETPQE EAKVDETKI VETPEQTKV EDKPAEETE 
      IEDKLKEIE IETKLKKTV IEDKLEETK VEDKPEETK 
      IDDKPEETK IDDKPEETK EQPMETVTE EAPEPEPEN 
      KTVPEEVKK VEEPKKRPD APKTKKKVP KPKEPKKNP 
      PKKKAAKPK PKKEPTDKT EEVRRSSRI KSINVLKQR 
      SKGHGLVKS AKLPPDENK ESATTQSSE EKSESATSL 
      PATEADNKP VKVKSRWRR SSELEMNVL TPAAIVMET 
      CGDSEKIAE ANNLENKTK PTHDEEVTT RLKQFVHLK 
      ENLYLTDRM SCKEAKKMT CDCFLTPEE IERGELGCG 
      EDCLNRLLM IECGGLCPV GDRCTNKKF QKSQFAPVE 
      VFKTEKKGL GLRAAANIP YGEFILEYV GEVLDPEEF 
      DNRADDYSN DKNKHYYFM SLRADAIID ATMKGNISR 
      FINHSCDPN AETQKWTVN GELRIGFFS TRTILAGEE 
      ITFDYRFQR YGKEAQKCY CESSLCRGW LGEEPDDED 
      DEDEEDEEE EEAEETKDK VEEDKPEAE KIDSEAKET 
      KSVEKVQKP PVKEKKKRS PRKKPRKEL LEDLDQLDD 
      EIDMLVTTG LKNQAHTLK LSRLMVRAK EPGQRAKLL 
      RVLRRGELP CRRLFLDYH GLRLMHGYM TDAQDLAKT 
      DKKYESLRL EMLQTLATL PIPNKTMLQ DSKVLPTVE 
      IWSSGKVNE LPVDSDSSS PQTDQEPSQ TPKEDDKKK 
      EENVKEEEE VKDVKMDED VEIRKSRGM EFQGSSENV 
      GFTNTHGKE LADEISSIF QEEDNTSSP TPPETKIVK 
      PEVKSDEPN YQEEITALA LKLLEEWSN LKEVFKIPK 
      KERIEQMKE HEREANRGY IAGLGLEQD AEKKNESRY 
      RILKRYRSI DKVDNSARK NIKLEDRTI ANFPKLGKY 
      ERRKMFALQ VEQREEERR RKQREMWRQ HEQHCILLG 
      TDPRLTAPF DPNRGYQCI WNPQIGQWQ NYPIQNPNV 
      IFNPQNPPV VSGMNNPNY PYLHPPGMI PNNVNPLHA 
      LPSLPYQEE VTKEEDYSQ VKFMGPIPP PVKLPPKWK 
      CAKDKYGRP YYYHIKIRK SQWEPPPFS QPQEEDLDD 
      TSETSSSSD TSSLSSDTT SDESDSDDD LDDSKLLMQ 
      VKRQMEKQV KIALPAPVK TEEDEASPV VEDKEEETK 
      LETQVCEEN SEETPQVEE NNDEKAQPG AKKRRTGLV 
      QEIIISPRT EEDRLQFKE DLKRYKLNK EKLKREKEM 
      IVQQTKKHA KEKRKSTIK LKMREMSDS NSETAKKVK 
      EVFRSNMAT VVVSVLNAY RKPDCKEGR ITNTDDFKH 
      LARKLTHFV MLKEMKHIE KIDDLVCTE NVKAKAKEY 
      IRKYMSKFG ENYQKRNDE PDY
    CDS sequence
    • >TC014310 RA
      atggcaccg aagaagaag cgtgggggg caaaaccga 
      gtcactttt gcaccgagt aaaatgtca acaaggtct 
      ggtaaaaag accgctaca agtccaaaa accagcaaa 
      gcaaaagtg ccgaaaatg cccaaattg gccacgaag 
      gcgacagct aagaaggga aaagcggtc cctaaagcc 
      aaaaaagct gcaccgaaa aaaaccaaa attgaggtt 
      acgtcaaca gactctgag ccactcgaa atggagtcg 
      gaaattgat agcaaaaac aacgatgag gatttacaa 
      ccagaaccc atcgagcag gttttcctc aaaatggat 
      gataatttc atggaaacg actgaacca gaaggtgaa 
      gaaactgag acttgtgaa attagagaa cagctcgaa 
      agtctcaat gtggaggag gcccacgaa cccaaacca 
      gggaatgag gaggtgaat gagcatagt agtgaagaa 
      agtgtggtt gaagaagta atagagatt gatgggtgt 
      ggagaatat gagtacatc accgaggaa ttcgtccca 
      gttgtggaa aacgccgaa gtgataatt atagacgat 
      gaagtggag gcaaataat caagtgcaa gaaaagccg 
      gaacaaata ataagttcc gaggaaaaa attcaagaa 
      atgaagctt gacgaaaca ccgcaagaa gaggctaaa 
      gttgacgaa acaaaaatt gtggagaca cctgaacaa 
      acaaaagta gaggataaa ccagccgaa gaaacagag 
      attgaagat aaacttaaa gaaatcgag attgagacc 
      aaactgaaa aaaaccgtg attgaggat aaacttgag 
      gaaacaaag gttgaggat aaacctgaa gaaaccaaa 
      atagacgat aagcctgaa gaaaccaag atagacgat 
      aaacctgaa gaaacgaaa gaacaacca atggaaact 
      gtaacagag gaggcacct gaacctgaa cccgaaaat 
      aaaacagtt ccagaggaa gttaagaaa gttgaagag 
      cccaaaaaa cgccctgac gcccccaaa acgaagaag 
      aaggtccct aagcccaaa gaacccaag aagaaccct 
      ccaaaaaag aaagccgcg aagcccaaa ccgaaaaaa 
      gaacccacc gataaaaca gaagaagtg aggcgttcg 
      agtcggatc aagtcaatt aatgtccta aagcagcgc 
      tcaaaaggc cacggttta gtaaaatca gccaaactc 
      ccccctgac gaaaacaaa gaatcagca actacccag 
      tcaagtgaa gaaaagtcc gaatcggcc acttcactc 
      ccagccacc gaagccgac aacaaacca gtcaaagtc 
      aaatctcga tggaggaga tcgagcgaa ttagaaatg 
      aacgttctt acaccggct gcgattgtg atggagact 
      tgcggagac agtgaaaaa attgcagaa gccaataat 
      ttggaaaat aaaacgaaa ccaactcac gacgaagaa 
      gtcaccacg cgattgaaa cagtttgta catttgaag 
      gaaaatctc tatttgact gatcgcatg agctgcaag 
      gaggcgaag aaaatgacc tgtgattgc ttcctgacg 
      ccggaggag attgaaagg ggggaattg ggctgtggg 
      gaagattgc ttaaatcgc ttactaatg atagaatgt 
      gggggttta tgcccagtt ggagacaga tgtaccaat 
      aaaaaattc caaaaatcg cagtttgcc ccagttgag 
      gtttttaaa accgagaag aagggactg gggttgaga 
      gctgcagcc aacattcct tatggtgaa tttattctg 
      gaatatgtc ggcgaagta ttggaccct gaagagttt 
      gataaccgt gccgacgac tattcaaac gataaaaac 
      aaacattac tacttcatg tcgttacga gccgatgcg 
      ataatagac gcaacaatg aaaggaaat atctcacgt 
      tttattaac cattcctgt gacccaaac gctgaaact 
      caaaaatgg actgttaat ggcgagtta cgtatcggt 
      tttttcagc acacgtacg atcttagcg ggtgaagaa 
      atcaccttt gattaccga tttcaacgt tatggaaaa 
      gaggcgcaa aagtgttac tgcgagtcg tcgctttgt 
      cgcggttgg ctgggtgaa gaaccggac gatgaagac 
      gacgaagac gaggaggac gaagaagag gaagaagct 
      gaggaaact aaagacaaa gtcgaggaa gataaacca 
      gaagctgag aaaatcgac agtgaagca aaagaaacc 
      aagagtgtt gaaaaagtg caaaaacct ccagtgaaa 
      gagaagaag aagagatcg ccacggaag aaaccgaga 
      aaagaattg ttggaggac ctagatcaa ctggacgac 
      gaaatagac atgttggtg accacaggg ttgaagaat 
      caagctcac actttaaaa ctgagcaga ttgatggtc 
      agagccaag gagcccggc caacgggcg aagctattg 
      agagtctta agaaggggc gaattacca tgcagacga 
      cttttcttg gactatcat gggttgaga ctcatgcat 
      ggctacatg accgacgcc caagacctg gcaaagact 
      gacaaaaaa tacgaatca ctcagattg gagatgtta 
      caaacgtta gcaacgctg ccaatcccc aacaaaacg 
      atgctgcaa gacagtaaa gtgttacca actgttgaa 
      atatggtcc tctggtaaa gttaacgag ttgccggtg 
      gattctgat agttcttcg cctcaaacc gatcaggag 
      ccaagtcaa acacccaaa gaagatgat aagaaaaaa 
      gaggaaaat gtgaaggag gaagaagaa gtgaaagat 
      gttaaaatg gatgaggat gtggaaata cgcaagtcc 
      agaggaatg gaattccag ggtagctcg gaaaatgtg 
      ggttttacg aacacacat gggaaggaa ttagccgac 
      gaaatttcg agcattttc caagaagaa gacaatact 
      tcgtcaccc acaccaccg gagaccaaa atcgtgaaa 
      cccgaggtg aaatctgac gagcctaat taccaggag 
      gagatcaca gctttggcc ttaaaactg ttggaagag 
      tggtccaac ttgaaggaa gtcttcaaa attcccaaa 
      aaagaacga atcgaacaa atgaaagag cacgaacgt 
      gaggccaac cgtggctac atcgccggt ttggggttg 
      gaacaagac gccgaaaag aaaaacgaa agtcgctat 
      cggatttta aaacggtac agatccatt gataaagtc 
      gataacagc gcgcgaaaa aacatcaaa ctggaagac 
      cggacgata gccaacttc cccaaattg ggcaaatac 
      gagcggagg aaaatgttt gcgttgcaa gtggagcaa 
      agggaggag gagaggcgg cgaaaacag agggagatg 
      tggaggcag catgagcaa cactgtatt ctcctcggg 
      acggatccc cgcctgaca gccccattt gaccccaac 
      agggggtac cagtgcata tggaacccc caaatcggg 
      caatggcaa aactacccc atccaaaat cccaatgtt 
      attttcaac ccccaaaat ccacccgtt gtctcaggg 
      atgaacaac ccgaattat ccctacttg caccctccg 
      ggaatgatc ccgaacaac gtgaacccg ctgcacgca 
      ctacccagt cttccatac caggaggaa gtgaccaaa 
      gaggaggac tactcacaa gttaagttc atgggaccc 
      attccgccc ccagtcaaa ctaccaccg aaatggaaa 
      tgtgccaaa gacaagtac ggacggcct tattattac 
      cacatcaag atcaggaaa agtcagtgg gaaccgcca 
      ccgttttcg caaccacag gaggaagac ttggatgac 
      acgagcgaa acgtcttca agctctgac acgagtagc 
      ttgagctcg gacacgacc tcggacgag tcagacagt 
      gacgatgat ttggacgac tccaaactg ttgatgcag 
      gtgaaaaga cagatggaa aaacaagta aaaattgca 
      ttacccgcc cctgttaaa accgaagaa gatgaagct 
      agtcccgtg gtcgaggac aaggaagaa gagactaaa 
      cttgaaact caagtttgt gaggaaaat agcgaagaa 
      acacctcag gttgaggaa aacaatgac gagaaagcg 
      caaccaggg gctaagaag cgacgaact ggactggtc 
      caggaaatt atcattagt ccgaggact gaagaggac 
      cgattgcaa ttcaaggaa gacttgaag cgttacaaa 
      ctaaataaa gaaaagttg aagcgggag aaggaaatg 
      atcgtccag caaacaaaa aaacacgcg aaggagaag 
      cgcaaaagt actattaaa ttgaaaatg agggaaatg 
      agtgattcc aacagcgaa actgccaag aaagttaag 
      gaggttttc cgttcgaat atggcgact gttgtcgtc 
      agtgttctt aacgcgtat agaaagccg gattgtaaa 
      gaagggaga attacgaat acagatgat tttaagcat 
      ttagctaga aaattgact cactttgtt atgttgaaa 
      gaaatgaaa catattgag aagattgat gatttagtc 
      tgcactgaa aacgtgaaa gcgaaagct aaggaatac 
      atccgcaaa tatatgagt aagttcgga gaaaattat 
      cagaagcga aatgatgag cccgattat taa
    mRNA sequence
    • >TC014310 RA
      gtggccgag ctccaactg tgcaaacat ccctaaatg 
      ttgtgagtg gttgtgttg ttaagaaat catgttttt 
      acgtgttta ggagcaaat gttggttat tttaatcga 
      aaacaagtg ggatggtca gccatttgg cccattgag 
      agtttgcta gtgtgtcag ttatgcacg ggaataatt 
      aggcgattt atgtgcaag acagtaaaa tcggctcta 
      ctaacgcaa agttgattc atcatggca ccgaagaag 
      aagcgtggg gggcaaaac cgagtcact tttgcaccg 
      agtaaaatg tcaacaagg tctggtaaa aagaccgct 
      acaagtcca aaaaccagc aaagcaaaa gtgccgaaa 
      atgcccaaa ttggccacg aaggcgaca gctaagaag 
      ggaaaagcg gtccctaaa gccaaaaaa gctgcaccg 
      aaaaaaacc aaaattgag gttacgtca acagactct 
      gagccactc gaaatggag tcggaaatt gatagcaaa 
      aacaacgat gaggattta caaccagaa cccatcgag 
      caggttttc ctcaaaatg gatgataat ttcatggaa 
      acgactgaa ccagaaggt gaagaaact gagacttgt 
      gaaattaga gaacagctc gaaagtctc aatgtggag 
      gaggcccac gaacccaaa ccagggaat gaggaggtg 
      aatgagcat agtagtgaa gaaagtgtg gttgaagaa 
      gtaatagag attgatggg tgtggagaa tatgagtac 
      atcaccgag gaattcgtc ccagttgtg gaaaacgcc 
      gaagtgata attatagac gatgaagtg gaggcaaat 
      aatcaagtg caagaaaag ccggaacaa ataataagt 
      tccgaggaa aaaattcaa gaaatgaag cttgacgaa 
      acaccgcaa gaagaggct aaagttgac gaaacaaaa 
      attgtggag acacctgaa caaacaaaa gtagaggat 
      aaaccagcc gaagaaaca gagattgaa gataaactt 
      aaagaaatc gagattgag accaaactg aaaaaaacc 
      gtgattgag gataaactt gaggaaaca aaggttgag 
      gataaacct gaagaaacc aaaatagac gataagcct 
      gaagaaacc aagatagac gataaacct gaagaaacg 
      aaagaacaa ccaatggaa actgtaaca gaggaggca 
      cctgaacct gaacccgaa aataaaaca gttccagag 
      gaagttaag aaagttgaa gagcccaaa aaacgccct 
      gacgccccc aaaacgaag aagaaggtc cctaagccc 
      aaagaaccc aagaagaac cctccaaaa aagaaagcc 
      gcgaagccc aaaccgaaa aaagaaccc accgataaa 
      acagaagaa gtgaggcgt tcgagtcgg atcaagtca 
      attaatgtc ctaaagcag cgctcaaaa ggccacggt 
      ttagtaaaa tcagccaaa ctcccccct gacgaaaac 
      aaagaatca gcaactacc cagtcaagt gaagaaaag 
      tccgaatcg gccacttca ctcccagcc accgaagcc 
      gacaacaaa ccagtcaaa gtcaaatct cgatggagg 
      agatcgagc gaattagaa atgaacgtt cttacaccg 
      gctgcgatt gtgatggag acttgcgga gacagtgaa 
      aaaattgca gaagccaat aatttggaa aataaaacg 
      aaaccaact cacgacgaa gaagtcacc acgcgattg 
      aaacagttt gtacatttg aaggaaaat ctctatttg 
      actgatcgc atgagctgc aaggaggcg aagaaaatg 
      acctgtgat tgcttcctg acgccggag gagattgaa 
      aggggggaa ttgggctgt ggggaagat tgcttaaat 
      cgcttacta atgatagaa tgtgggggt ttatgccca 
      gttggagac agatgtacc aataaaaaa ttccaaaaa 
      tcgcagttt gccccagtt gaggttttt aaaaccgag 
      aagaaggga ctggggttg agagctgca gccaacatt 
      ccttatggt gaatttatt ctggaatat gtcggcgaa 
      gtattggac cctgaagag tttgataac cgtgccgac 
      gactattca aacgataaa aacaaacat tactacttc 
      atgtcgtta cgagccgat gcgataata gacgcaaca 
      atgaaagga aatatctca cgttttatt aaccattcc 
      tgtgaccca aacgctgaa actcaaaaa tggactgtt 
      aatggcgag ttacgtatc ggttttttc agcacacgt 
      acgatctta gcgggtgaa gaaatcacc tttgattac 
      cgatttcaa cgttatgga aaagaggcg caaaagtgt 
      tactgcgag tcgtcgctt tgtcgcggt tggctgggt 
      gaagaaccg gacgatgaa gacgacgaa gacgaggag 
      gacgaagaa gaggaagaa gctgaggaa actaaagac 
      aaagtcgag gaagataaa ccagaagct gagaaaatc 
      gacagtgaa gcaaaagaa accaagagt gttgaaaaa 
      gtgcaaaaa cctccagtg aaagagaag aagaagaga 
      tcgccacgg aagaaaccg agaaaagaa ttgttggag 
      gacctagat caactggac gacgaaata gacatgttg 
      gtgaccaca gggttgaag aatcaagct cacacttta 
      aaactgagc agattgatg gtcagagcc aaggagccc 
      ggccaacgg gcgaagcta ttgagagtc ttaagaagg 
      ggcgaatta ccatgcaga cgacttttc ttggactat 
      catgggttg agactcatg catggctac atgaccgac 
      gcccaagac ctggcaaag actgacaaa aaatacgaa 
      tcactcaga ttggagatg ttacaaacg ttagcaacg 
      ctgccaatc cccaacaaa acgatgctg caagacagt 
      aaagtgtta ccaactgtt gaaatatgg tcctctggt 
      aaagttaac gagttgccg gtggattct gatagttct 
      tcgcctcaa accgatcag gagccaagt caaacaccc 
      aaagaagat gataagaaa aaagaggaa aatgtgaag 
      gaggaagaa gaagtgaaa gatgttaaa atggatgag 
      gatgtggaa atacgcaag tccagagga atggaattc 
      cagggtagc tcggaaaat gtgggtttt acgaacaca 
      catgggaag gaattagcc gacgaaatt tcgagcatt 
      ttccaagaa gaagacaat acttcgtca cccacacca 
      ccggagacc aaaatcgtg aaacccgag gtgaaatct 
      gacgagcct aattaccag gaggagatc acagctttg 
      gccttaaaa ctgttggaa gagtggtcc aacttgaag 
      gaagtcttc aaaattccc aaaaaagaa cgaatcgaa 
      caaatgaaa gagcacgaa cgtgaggcc aaccgtggc 
      tacatcgcc ggtttgggg ttggaacaa gacgccgaa 
      aagaaaaac gaaagtcgc tatcggatt ttaaaacgg 
      tacagatcc attgataaa gtcgataac agcgcgcga 
      aaaaacatc aaactggaa gaccggacg atagccaac 
      ttccccaaa ttgggcaaa tacgagcgg aggaaaatg 
      tttgcgttg caagtggag caaagggag gaggagagg 
      cggcgaaaa cagagggag atgtggagg cagcatgag 
      caacactgt attctcctc gggacggat ccccgcctg 
      acagcccca tttgacccc aacaggggg taccagtgc 
      atatggaac ccccaaatc gggcaatgg caaaactac 
      cccatccaa aatcccaat gttattttc aacccccaa 
      aatccaccc gttgtctca gggatgaac aacccgaat 
      tatccctac ttgcaccct ccgggaatg atcccgaac 
      aacgtgaac ccgctgcac gcactaccc agtcttcca 
      taccaggag gaagtgacc aaagaggag gactactca 
      caagttaag ttcatggga cccattccg cccccagtc 
      aaactacca ccgaaatgg aaatgtgcc aaagacaag 
      tacggacgg ccttattat taccacatc aagatcagg 
      aaaagtcag tgggaaccg ccaccgttt tcgcaacca 
      caggaggaa gacttggat gacacgagc gaaacgtct 
      tcaagctct gacacgagt agcttgagc tcggacacg 
      acctcggac gagtcagac agtgacgat gatttggac 
      gactccaaa ctgttgatg caggtgaaa agacagatg 
      gaaaaacaa gtaaaaatt gcattaccc gcccctgtt 
      aaaaccgaa gaagatgaa gctagtccc gtggtcgag 
      gacaaggaa gaagagact aaacttgaa actcaagtt 
      tgtgaggaa aatagcgaa gaaacacct caggttgag 
      gaaaacaat gacgagaaa gcgcaacca ggggctaag 
      aagcgacga actggactg gtccaggaa attatcatt 
      agtccgagg actgaagag gaccgattg caattcaag 
      gaagacttg aagcgttac aaactaaat aaagaaaag 
      ttgaagcgg gagaaggaa atgatcgtc cagcaaaca 
      aaaaaacac gcgaaggag aagcgcaaa agtactatt 
      aaattgaaa atgagggaa atgagtgat tccaacagc 
      gaaactgcc aagaaagtt aaggaggtt ttccgttcg 
      aatatggcg actgttgtc gtcagtgtt cttaacgcg 
      tatagaaag ccggattgt aaagaaggg agaattacg 
      aatacagat gattttaag catttagct agaaaattg 
      actcacttt gttatgttg aaagaaatg aaacatatt 
      gagaagatt gatgattta gtctgcact gaaaacgtg 
      aaagcgaaa gctaaggaa tacatccgc aaatatatg 
      agtaagttc ggagaaaat tatcagaag cgaaatgat 
      gagcccgat tattaattt cattcattt tttatttgt 
      ctgtgataa aaatcattt catttttca acactgtac 
      gagtcgata agtattgta aaaatgtat gaataattt 
      ataataaaa taggtgaat acgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY