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Gene information
General information
TC013501 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8737799..8791989
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013501
    iBeetle screen iB_10041

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature thinner - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013501 RA
      MSRRKQARP SRLLEDDDA PAFPAAAGL DEASPCMDR 
      DMSLTSSQD LPEVLMCQR CQEQFTDVE EYLTHKAEC 
      EKKAIKHDD PAHSDPEDM VVSEEDDED DDAGGKRLE 
      RMRRHRQDA ANNNSVEEN SPEDTSPRL SFPIPIPSA 
      PGHVTLEAL QNTKVAVAQ FAATAMANN TDNEAALQE 
      LAVLQSTLF TLQHQQVLQ LQLINQLQQ QLQIDRSKA 
      ASPVSPPPS ENGENAPAE ASPPPQNLP VSREPTPTP 
      IIQPPQPIV QEPSENQTT EMNVPCSLP LQSQHCSIS 
      SSLASTIIT HNNEPPSLD EPNTLEMLQ KRAQEVLDN 
      ASQGLLATN LADELAFRR NKGSLSPYD SKGRNEPFF 
      KHRCRYCGK VFGSDSALQ IHIRSHTGE RPYKCNVCG 
      SRFTTKGNL KVHFQRHSA KFPHIKMNP NPVPEHLDK 
      YHPPLLAQL GQQPSLSPG GPPPHMGFP GGHPFPPTS 
      LPLYRPHGP PPDLLNSRL QPSPHRPQD PPQRLFPPH 
      PLFMKREEQ EAPENLTKP ARSPTPVRD THCKSEISD 
      EKREYDDAQ SNVPQITPK QEPNDEGEH EPERYSSPA 
      PYDECSIDS KYSNEDTLG ARSPGGDHS ENMQDEPEN 
      LSNKSNSIT VPLSISTGQ RLPANFSFG QVNSPPSST 
      SSGSLGQFP ATPVIDPAK DPAIYSNLL PRPGSNDNS 
      WESLIEVTK TSETSKLQQ LVDNIEHKL SDPNQCVIC 
      HRVLSCKSA LQMHYRTHT GERPFKCKI CGRAFTTKG 
      NLKTHMGVH RAKPPMRVL HQCPVCHKK FTNALVLQQ 
      HIRLHTGEP TDLTPEQIQ AAEVKDFPS PGGFPSIHN 
      SINPFLGQG FAVPGLSPL GHTLSMNHK YEKIDKEEH 
      ESMDDDDDD DDDMSNSEN QNPRFTSSP DMQGVPTSM 
      ASQLSMSGL SCSAEDLCS TRTAASASP MQNGEKSPC 
      QSGITSPGS SEIRASPNR VTTTPVQVP RPPSSQHAG 
      SPTPSECNS LGALDLTPR TNQPLITSP GPSPGPPPA 
      LFSTFGLIP PGQGSTPLM SSALSSLTS SVLTSTAFS 
      PLRLAVGPT GRGNTTCNL CFKTFACNS ALEIHYRSH 
      TKERPFKCS ICDRGFSTK DGCVCRERR IQDEAEPWK 
      ASKRKPASR KSIPSVLPL PMSPGYASN WMHIAGNMK 
      QHMLTHKIR DMPQHMFEN KPPISGDEN SQQSQQNLP 
      QREVQPSEN EQPNPPSQP SISEQNKEQ PVKREPTET 
      ELPLPKRPP SLQASKHLC HVCNKNFSS SSALQIHMR 
      THTGDKPFR CTVCQKAFT TKGNLKVHM GTHMWSNGA 
      SRRGRRMSL DLPPIPMTP KDSEFLQRR PDLFYPYLP 
      APFLNGMQQ KLNEMSVIQ NNQNGMSAA GKYSGLLGF 
      GYPPPEALR SQGGSPDRP ERPPSRDPE SRSLWDLHF 
      ERKSAPEAP REELLPASR EGLAA
    CDS sequence
    • >TC013501 RA
      atgtctcgc aggaagcaa gcgaggccc agccgcctc 
      ctcgaggac gacgacgct ccggccttc cccgccgct 
      gcgggcctc gatgaagca tcgccatgt atggaccgg 
      gatatgtcg ctgacatcg tcacaagac ctacctgaa 
      gtactgatg tgtcagcga tgtcaagaa cagttcacc 
      gacgttgaa gaatatctc acgcacaag gccgaatgt 
      gagaagaag gcaatcaag cacgacgat ccggctcac 
      tctgacccc gaggacatg gtggtttcc gaagaggac 
      gacgaagat gacgacgca ggcggcaaa cgtttggaa 
      cgtatgcga cgtcatcga caggacgcc gccaataac 
      aacagtgtc gaagaaaac tcccctgag gacaccagc 
      cccaggctg tctttcccc atcccaatc ccgtcggca 
      ccgggccat gtgacgttg gaagccctt cagaacact 
      aaagtggca gttgcgcaa ttcgcagcg acggcaatg 
      gccaacaat acggacaac gaagcggct ctccaagaa 
      ctagcagtg ttgcagtca acactcttc accctccag 
      caccaacaa gtgttgcag ttacaactc atcaatcag 
      ctgcaacag cagttgcag attgacaga tctaaagct 
      gcaagtccc gtctcgccc ccaccttcc gaaaacggg 
      gaaaatgcc ccggctgaa gcttcgcca cccccacaa 
      aatttgccc gtttcaagg gaaccaacc ccaactccc 
      ataatacag cccccacaa cccatcgta caagaacca 
      tccgagaac caaacgact gaaatgaat gttccttgc 
      tctctacct ctgcagtcg cagcactgt tccatatca 
      tcatcacta gcttcaact ataatcact cacaataac 
      gaaccacct tcattagac gaaccaaat acgttagaa 
      atgttgcaa aagagagct caagaggtc ttagataac 
      gccagtcag gggttattg gccacaaat ctggccgac 
      gaactggcc tttaggaga aacaaaggc tcgttatcg 
      ccttatgac tccaaaggg cggaacgaa ccgttcttc 
      aaacaccgg tgtcggtac tgcggaaaa gtcttcggc 
      tctgactcg gccctgcaa atacatatc cgttcccac 
      acgggagaa cgcccctac aagtgcaat gtttgtggt 
      agtcggttt accacgaaa ggtaacctg aaagtacat 
      tttcaaaga catagtgca aagtttcct catatcaaa 
      atgaacccg aatccggtc ccggaacac ctagacaag 
      tatcatcca ccgctctta gctcaatta ggacaacag 
      ccctcgctg tcgccgggc ggtccccca ccacatatg 
      ggttttccc ggaggtcac cccttccca cccacctca 
      ttgcccctg taccgaccc cacggcccc ccgcccgat 
      ttgctaaac agtcggctg caaccttca ccgcacagg 
      cctcaagac ccgccgcaa aggctcttc ccaccgcat 
      ccccttttc atgaagcga gaggaacaa gaggctcct 
      gagaatctc accaaaccg gcaagatcg ccaactccc 
      gtgcgagac actcactgc aaatcggaa atttccgac 
      gaaaaacgc gaatatgac gatgcacaa tccaacgtt 
      cctcagata acaccaaaa caagaacca aacgacgaa 
      ggcgaacac gaacctgag cgatactcc tctccggcg 
      ccctacgac gaatgcagc atcgacagc aagtacagc 
      aacgaggac acgctggga gcgcgaagc ccgggagga 
      gaccattcg gagaatatg caagatgag cctgagaat 
      ctctcgaat aagagcaat tccataaca gttcctttg 
      agcatttcc acgggtcag agattgcct gcgaacttt 
      tcgttcgga caagtgaat tcgccgccg agtagtaca 
      tcgtcggga agcttaggc caatttcca gcaacgcca 
      gtgatcgat ccagccaaa gaccccgct atctactcg 
      aatttattg ccacggcca gggagcaac gacaattca 
      tgggagagt ttgatagaa gtgacaaaa acgtcggaa 
      accagcaaa ctccaacag cttgttgac aatatcgaa 
      cataaactt tccgatccg aatcagtgt gtgatatgt 
      catcgagtg ttgtcgtgc aagagcgcc ttacagatg 
      cattatcga actcacacc ggcgaacgg ccgttcaaa 
      tgtaagatt tgtggacgg gctttcacc accaagggc 
      aatctcaag acacacatg ggggtgcac agggccaag 
      cccccgatg cgggtgctg caccagtgc ccggtctgc 
      cacaaaaag ttcaccaat gcgcttgtt ctgcaacaa 
      catatccgc cttcacacg ggtgaaccc actgatctc 
      actcccgag cagattcaa gcggctgaa gtgaaagac 
      tttccgtcg ccgggagga ttcccttcg attcataat 
      tccattaat ccattccta gggcaaggg tttgccgtt 
      ccggggctg tcgccctta ggtcacaca ctctcgatg 
      aatcacaaa tatgaaaaa atcgataaa gaggaacac 
      gaatcgatg gacgacgat gatgacgac gacgatgac 
      atgagcaac tccgaaaat caaaacccg aggtttact 
      tcctcgcca gatatgcaa ggagtgccg actagcatg 
      gcttcccag ttgtcaatg agtggttta agttgctct 
      gccgaagat ttatgttct actaggact gcggcttcg 
      gcttcgccg atgcaaaat ggggagaaa tcgccttgc 
      caatctgga atcacaagt ccaggcagt tcggagatt 
      cgagcttcg ccaaaccgc gtcacaacg acacccgtc 
      caggttcca cgtccacct tcctctcaa catgccggc 
      agtccgact ccttccgag tgcaattct ttgggtgct 
      ttagactta acccctcga actaaccag cctttgata 
      acgagtccg ggtcccagt cccggcccg ccaccggct 
      ttgttttca acgttcgga ttgatacca ccaggtcaa 
      ggttccact cctttgatg tcatcggca ctttcatct 
      ttgacgtcg tctgttttg acatccaca gcgttcagt 
      ccattgcgt ttagccgtt ggaccaaca ggccgtggg 
      aacaccacc tgtaacttg tgtttcaag acgttcgct 
      tgcaactct gccttggag attcattac aggagtcac 
      acgaaagaa cgtcctttt aaatgcagt atctgcgat 
      agaggcttc tcgaccaag gacggatgt gtatgcaga 
      gagaggcgt atccaggat gaagcggag ccttggaag 
      gcaagcaaa aggaaaccg gcttctcgc aagtcaata 
      ccatcggta cttccgcta ccgatgagc cctggatac 
      gcctcaaac tggatgcat atcgcgggc aacatgaag 
      caacacatg cttacacat aaaataagg gacatgcct 
      caacacatg tttgagaat aaaccgccg ataagcgga 
      gatgaaaac tcgcaacaa tcccaacaa aacttacct 
      caaagggaa gttcaaccc agcgaaaac gaacaaccc 
      aatccaccc tcacaacct tctatatct gaacaaaac 
      aaagagcaa ccggttaaa agggaaccc accgagacg 
      gaactaccc ttgcccaaa cgtccaccc agcttacag 
      gcaagtaag cacttgtgc cacgtatgc aataagaac 
      ttctcgtcc agctcggcc ttgcagata cacatgcgt 
      acgcacacg ggcgataag ccgttcaga tgcaccgtg 
      tgccagaag gccttcacc accaagggg aacctcaag 
      gtacatatg ggaacgcat atgtggagc aacggggcg 
      tcgcgacgc ggccgtcgc atgtccctc gacctccct 
      cccattccc atgacaccc aaggattcc gaatttctg 
      caacggagg cccgacctt ttttatccg tacctaccg 
      gccccattc cttaacggc atgcagcag aagctgaac 
      gaaatgtca gttatacag aataaccag aacgggatg 
      agcgcggcg ggcaagtac agcgggctt ctcggcttc 
      ggctaccct ccgccggag gcgctgcgg tcgcagggc 
      ggctccccc gaccggccc gagaggccc ccgagccgc 
      gacccggag tcccggagc ctgtgggac ctccacttc 
      gagcgaaaa tcagccccg gaagccccg cgagaggag 
      ctgctgccc gcgagcagg gagggtttg gcggcctga
    mRNA sequence
    • >TC013501 RA
      atatcggat tcaaggcag attccagca cgatcgaat 
      ggtaaaaac ccgagtgtg atcgtcctc gtggtggag 
      gtacctata ggtatcgga gggtatggt ttttgggaa 
      tttcgtgag ttcgtagag ggtgcatgg agtgtcttg 
      tcgtccagc ggacattaa tccgcgcca gaatatcat 
      taagatact ccggcaata tcatcagtg atttatgaa 
      tcggtgttg gacttggcg gtgcgttga atgagtagt 
      ccagcccca ctccccagg taaggccgg tgggcgggt 
      cctcgtggg gcaggccga gatagtcac ttagtcaaa 
      tgccgctcg tcgagtcgg ctgcttgtc ctgtcagtc 
      cgcaccgca ggcgttaag ctgtcagaa gaaccgtcg 
      cgacgcatc cagagggca gcgacgccg tctcctcgt 
      gctccttca ggaatgtcg tagtggtgt gtcgtcccg 
      tccgaggaa gactgcgcg tggccgtcg acaagaacc 
      gaccctttc cgagggtga gttcgacgc gccgccctt 
      atctaagcc cccaccacg gcgcggcgc tgaagcaga 
      cagcatcgc ggcgccgac gagttcgta cggcacctg 
      caccaatca cggccacat cataacttc atattccga 
      catgagtgc ttcttgtag ttaggtggt aaactggtg 
      acgattcag tgaactgtg attgtgatg tctcgcagg 
      aagcaagcg aggcccagc cgcctcctc gaggacgac 
      gacgctccg gccttcccc gccgctgcg ggcctcgat 
      gaagcatcg ccatgtatg gaccgggat atgtcgctg 
      acatcgtca caagaccta cctgaagta ctgatgtgt 
      cagcgatgt caagaacag ttcaccgac gttgaagaa 
      tatctcacg cacaaggcc gaatgtgag aagaaggca 
      atcaagcac gacgatccg gctcactct gaccccgag 
      gacatggtg gtttccgaa gaggacgac gaagatgac 
      gacgcaggc ggcaaacgt ttggaacgt atgcgacgt 
      catcgacag gacgccgcc aataacaac agtgtcgaa 
      gaaaactcc cctgaggac accagcccc aggctgtct 
      ttccccatc ccaatcccg tcggcaccg ggccatgtg 
      acgttggaa gcccttcag aacactaaa gtggcagtt 
      gcgcaattc gcagcgacg gcaatggcc aacaatacg 
      gacaacgaa gcggctctc caagaacta gcagtgttg 
      cagtcaaca ctcttcacc ctccagcac caacaagtg 
      ttgcagtta caactcatc aatcagctg caacagcag 
      ttgcagatt gacagatct aaagctgca agtcccgtc 
      tcgccccca ccttccgaa aacggggaa aatgccccg 
      gctgaagct tcgccaccc ccacaaaat ttgcccgtt 
      tcaagggaa ccaacccca actcccata atacagccc 
      ccacaaccc atcgtacaa gaaccatcc gagaaccaa 
      acgactgaa atgaatgtt ccttgctct ctacctctg 
      cagtcgcag cactgttcc atatcatca tcactagct 
      tcaactata atcactcac aataacgaa ccaccttca 
      ttagacgaa ccaaatacg ttagaaatg ttgcaaaag 
      agagctcaa gaggtctta gataacgcc agtcagggg 
      ttattggcc acaaatctg gccgacgaa ctggccttt 
      aggagaaac aaaggctcg ttatcgcct tatgactcc 
      aaagggcgg aacgaaccg ttcttcaaa caccggtgt 
      cggtactgc ggaaaagtc ttcggctct gactcggcc 
      ctgcaaata catatccgt tcccacacg ggagaacgc 
      ccctacaag tgcaatgtt tgtggtagt cggtttacc 
      acgaaaggt aacctgaaa gtacatttt caaagacat 
      agtgcaaag tttcctcat atcaaaatg aacccgaat 
      ccggtcccg gaacaccta gacaagtat catccaccg 
      ctcttagct caattagga caacagccc tcgctgtcg 
      ccgggcggt cccccacca catatgggt tttcccgga 
      ggtcacccc ttcccaccc acctcattg cccctgtac 
      cgaccccac ggccccccg cccgatttg ctaaacagt 
      cggctgcaa ccttcaccg cacaggcct caagacccg 
      ccgcaaagg ctcttccca ccgcatccc cttttcatg 
      aagcgagag gaacaagag gctcctgag aatctcacc 
      aaaccggca agatcgcca actcccgtg cgagacact 
      cactgcaaa tcggaaatt tccgacgaa aaacgcgaa 
      tatgacgat gcacaatcc aacgttcct cagataaca 
      ccaaaacaa gaaccaaac gacgaaggc gaacacgaa 
      cctgagcga tactcctct ccggcgccc tacgacgaa 
      tgcagcatc gacagcaag tacagcaac gaggacacg 
      ctgggagcg cgaagcccg ggaggagac cattcggag 
      aatatgcaa gatgagcct gagaatctc tcgaataag 
      agcaattcc ataacagtt cctttgagc atttccacg 
      ggtcagaga ttgcctgcg aacttttcg ttcggacaa 
      gtgaattcg ccgccgagt agtacatcg tcgggaagc 
      ttaggccaa tttccagca acgccagtg atcgatcca 
      gccaaagac cccgctatc tactcgaat ttattgcca 
      cggccaggg agcaacgac aattcatgg gagagtttg 
      atagaagtg acaaaaacg tcggaaacc agcaaactc 
      caacagctt gttgacaat atcgaacat aaactttcc 
      gatccgaat cagtgtgtg atatgtcat cgagtgttg 
      tcgtgcaag agcgcctta cagatgcat tatcgaact 
      cacaccggc gaacggccg ttcaaatgt aagatttgt 
      ggacgggct ttcaccacc aagggcaat ctcaagaca 
      cacatgggg gtgcacagg gccaagccc ccgatgcgg 
      gtgctgcac cagtgcccg gtctgccac aaaaagttc 
      accaatgcg cttgttctg caacaacat atccgcctt 
      cacacgggt gaacccact gatctcact cccgagcag 
      attcaagcg gctgaagtg aaagacttt ccgtcgccg 
      ggaggattc ccttcgatt cataattcc attaatcca 
      ttcctaggg caagggttt gccgttccg gggctgtcg 
      cccttaggt cacacactc tcgatgaat cacaaatat 
      gaaaaaatc gataaagag gaacacgaa tcgatggac 
      gacgatgat gacgacgac gatgacatg agcaactcc 
      gaaaatcaa aacccgagg tttacttcc tcgccagat 
      atgcaagga gtgccgact agcatggct tcccagttg 
      tcaatgagt ggtttaagt tgctctgcc gaagattta 
      tgttctact aggactgcg gcttcggct tcgccgatg 
      caaaatggg gagaaatcg ccttgccaa tctggaatc 
      acaagtcca ggcagttcg gagattcga gcttcgcca 
      aaccgcgtc acaacgaca cccgtccag gttccacgt 
      ccaccttcc tctcaacat gccggcagt ccgactcct 
      tccgagtgc aattctttg ggtgcttta gacttaacc 
      cctcgaact aaccagcct ttgataacg agtccgggt 
      cccagtccc ggcccgcca ccggctttg ttttcaacg 
      ttcggattg ataccacca ggtcaaggt tccactcct 
      ttgatgtca tcggcactt tcatctttg acgtcgtct 
      gttttgaca tccacagcg ttcagtcca ttgcgttta 
      gccgttgga ccaacaggc cgtgggaac accacctgt 
      aacttgtgt ttcaagacg ttcgcttgc aactctgcc 
      ttggagatt cattacagg agtcacacg aaagaacgt 
      ccttttaaa tgcagtatc tgcgataga ggcttctcg 
      accaaggac ggatgtgta tgcagagag aggcgtatc 
      caggatgaa gcggagcct tggaaggca agcaaaagg 
      aaaccggct tctcgcaag tcaatacca tcggtactt 
      ccgctaccg atgagccct ggatacgcc tcaaactgg 
      atgcatatc gcgggcaac atgaagcaa cacatgctt 
      acacataaa ataagggac atgcctcaa cacatgttt 
      gagaataaa ccgccgata agcggagat gaaaactcg 
      caacaatcc caacaaaac ttacctcaa agggaagtt 
      caacccagc gaaaacgaa caacccaat ccaccctca 
      caaccttct atatctgaa caaaacaaa gagcaaccg 
      gttaaaagg gaacccacc gagacggaa ctacccttg 
      cccaaacgt ccacccagc ttacaggca agtaagcac 
      ttgtgccac gtatgcaat aagaacttc tcgtccagc 
      tcggccttg cagatacac atgcgtacg cacacgggc 
      gataagccg ttcagatgc accgtgtgc cagaaggcc 
      ttcaccacc aaggggaac ctcaaggta catatggga 
      acgcatatg tggagcaac ggggcgtcg cgacgcggc 
      cgtcgcatg tccctcgac ctccctccc attcccatg 
      acacccaag gattccgaa tttctgcaa cggaggccc 
      gaccttttt tatccgtac ctaccggcc ccattcctt 
      aacggcatg cagcagaag ctgaacgaa atgtcagtt 
      atacagaat aaccagaac gggatgagc gcggcgggc 
      aagtacagc gggcttctc ggcttcggc taccctccg 
      ccggaggcg ctgcggtcg cagggcggc tcccccgac 
      cggcccgag aggcccccg agccgcgac ccggagtcc 
      cggagcctg tgggacctc cacttcgag cgaaaatca 
      gccccggaa gccccgcga gaggagctg ctgcccgcg 
      agcagggag ggtttggcg gcctgaaaa ttcggtgtt 
      cctatttca actagtcta agagcggat cggggcaat 
      gcatactgt gatacatca gccgtaaaa gctgcaagg 
      acagcgact aatactgct gtgttttct tagagttat 
      tttcattag agcctgtat tatagctaa gtgtatatg 
      actataact cgtatatcg tttgctgtg ttatattac 
      gtgtcgagg acactggcg atgccagtt actagcttt 
      acaacaccc tgtacataa ataggacta aatgcattt 
      gagaatcat tgtaccgta tgttagatg ttgtccatg 
      agtttgtta aacctgctt agcagggca cgtggcatt 
      ctatgtata tatcactct gtacgatga gactgtttc 
      atattattg atgtatgct ttcttccct cctcaaata 
      caaatcctg aaccactca ttacattcg actttcctc 
      tttcttgag ccaggttac tcacgctca tctactcta 
      catcaagtg acatcaata cgtatctca atcgataga 
      tattgtttg taaacatat gagatttat aaaataata 
      cagtgtaaa tgtaagtaa tttgaaatg tttttattt 
      caggatgaa tgtacaaat atttttaac tggtgcgct 
      atttaacta tgtctgtat ttataaact cgtacaaac 
      cagttaaac tctgtttaa ttataaaat ataaatatt 
      tatcacat
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