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Gene information
General information
TC033123 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8674337..8675530
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC033123
    iBeetle screen iB_10040

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC033123 RA
      MVVLEVFTK NIKISYKST LLSKASVLA LIFGVLSLV 
      LPFIFAYKS KGLWLKRDT FYEQPDVKF RGEYIFVAT 
      TNNYNKSVI TCSNLPHFD NVLRPLDTC SVIKVRETD 
      RNFDGKTEE LDLSLQVNL PVNTHLTSF CIIIPFNYK 
      LQVCPLEMQ TAIIFQELL PFYISKYSL VANVVVTQT 
      SPLICHRKN TNTLFNYPV IVDNGDLNN YDINTIITN 
      FSKRNISTS LRDVYKSFS PGKTQNFVL NLNIEFPED 
      RIYYKPGFW QVLKWAWIH TIFLAGD
    CDS sequence
    • >TC033123 RA
      atggtagtt ttagaagtt tttacaaag aatataaaa 
      atttcttat aaaagtact cttctatca aaagcatcg 
      gtgctagcc ctaattttt ggggtctta tcgcttgtg 
      ttaccattt atttttgca tacaaaagc aagggcttg 
      tggttaaaa agagacact ttttatgaa caaccggat 
      gtaaaattt cgaggagaa tacattttt gttgctact 
      acgaataat tacaataag tctgttata acgtgtagt 
      aatttaccc cactttgat aatgtattg cggccttta 
      gatacttgt tcagtcatc aaggttcgt gaaactgac 
      agaaatttt gatggtaaa acagaagaa ttagatttg 
      tctctccaa gttaatctt ccagttaat actcacctc 
      acgtctttt tgcataatc attccattt aattataaa 
      ttacaagtt tgtcctctt gaaatgcag actgccatt 
      atttttcaa gaattgtta ccattttat atatcgaag 
      tattcattg gtggctaat gtggttgtg acccaaact 
      agcccctta atttgccac agaaaaaac accaatacg 
      ttatttaat tatcctgtg attgtggat aatggggat 
      ttaaataac tatgacata aataccatt atcacaaac 
      ttttcaaag agaaacata tccacatca ttgagagac 
      gtctataaa agtttttct cctggaaaa actcaaaat 
      ttcgtatta aacttaaat atagaattt ccagaagac 
      aggatatac tacaagccg ggcttttgg caagtactg 
      aagtgggcc tggatccac actatattt ttagcagga 
      gattaa
    mRNA sequence
    • >TC033123 RA
      tgtttgtat tcaaagttg acaggtaaa caaataaaa 
      aattcgacc aaaaaccac aagacgtaa tttttgtga 
      tgttaatga tggtagttt tagaagttt ttacaaaga 
      atataaaaa tttcttata aaagtactc ttctatcaa 
      aagcatcgg tgctagccc taatttttg gggtcttat 
      cgcttgtgt taccattta tttttgcat acaaaagca 
      agggcttgt ggttaaaaa gagacactt tttatgaac 
      aaccggatg taaaatttc gaggagaat acatttttg 
      ttgctacta cgaataatt acaataagt ctgttataa 
      cgtgtagta atttacccc actttgata atgtattgc 
      ggcctttag atacttgtt cagtcatca aggttcgtg 
      aaactgaca gaaattttg atggtaaaa cagaagaat 
      tagatttgt ctctccaag ttaatcttc cagttaata 
      ctcacctca cgtcttttt gcataatca ttccattta 
      attataaat tacaagttt gtcctcttg aaatgcaga 
      ctgccatta tttttcaag aattgttac cattttata 
      tatcgaagt attcattgg tggctaatg tggttgtga 
      cccaaacta gccccttaa tttgccaca gaaaaaaca 
      ccaatacgt tatttaatt atcctgtga ttgtggata 
      atggggatt taaataact atgacataa ataccatta 
      tcacaaact tttcaaaga gaaacatat ccacatcat 
      tgagagacg tctataaaa gtttttctc ctggaaaaa 
      ctcaaaatt tcgtattaa acttaaata tagaatttc 
      cagaagaca ggatatact acaagccgg gcttttggc 
      aagtactga agtgggcct ggatccaca ctatatttt 
      tagcaggag attaatact gttttttga agaaaatcc 
      ggtgaagaa a
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY