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Gene information
General information
TC004120 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1742314..1743687
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC004120
    iBeetle screen iB_10036

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC004120 RA
      MQFYSYVFL YLLLPKTLS KRVEITCPQ HCKCDIFET 
      LRRATCTRG NLAGIESNI PTQTQILDL SFNRISWLQ 
      KNVFKEEKL TNLKLLNLS HNIVSHIEM KAFKGLSAL 
      RTLDLSYNS VQYLTPAWF RDMTALEEL YLRGNGFSK 
      LDSGPLFAS KSLKRLDLS LCRISYIGP DSFSQIPNL 
      EVLDVSENY LIHLYIATV EPLTNLQIL DAGNNSLSC 
      DGVSKNLAK YCVQKGIRF KDPCKPTIA DSEKFQRMV 
      NLAEPEDEK NSWIYDDGQ EIRHDNQTT TNATCNNTP 
      ADKKSLLQE IVDLSPVLS ILLPFIYGI AVGVLIAYI 
      LSVFSRKKQ SDEPAHDQN LRRSGTFSR MLSRGAVTE 
      ERQNLPLRE WHLSESTPV LFRKNQC
    CDS sequence
    • >TC004120 RA
      atgcagttt tattcatac gttttttta tatttattg 
      ctgcccaaa actttgagt aaacgagtc gaaattacg 
      tgcccgcaa cactgcaag tgtgatatt ttcgaaact 
      ttgagacgc gccacttgc acacgcggg aatttggca 
      gggattgag tcaaatata ccgacacaa acgcaaatt 
      ctggaccta tcctttaac cggattagc tggcttcag 
      aaaaatgtg tttaaggag gaaaaactc acgaatttg 
      aaactacta aacctgtcc cacaacata gtgagtcat 
      attgaaatg aaggctttc aaaggcttg agtgccttg 
      aggacgtta gatttgtcg tacaacagt gtccagtat 
      ttaacccca gcctggttt agggacatg acagcgctg 
      gaagaatta tatctgcga gggaacggc tttagtaaa 
      ctggacagt ggcccactt ttcgcctct aagtcactt 
      aaacgactt gacttgagc ctgtgccgc atatcttac 
      ataggcccg gactcattt agccagatc ccgaatctg 
      gaagtcttg gacgtgtct gagaactat ttgatccac 
      ctgtatatc gccacagtt gagcctttg acgaatctc 
      caaatcctg gatgccggt aacaattcg ttgtcctgt 
      gacggtgtc tcaaaaaac ttggcgaaa tattgcgtc 
      cagaaggga ataaggttc aaggatccg tgcaagccg 
      acgatcgcc gattcggaa aagttccag cgcatggtt 
      aatttggcc gagccggag gacgagaaa aattcatgg 
      atttatgac gacggccaa gagatacga catgacaat 
      caaacaaca acaaacgcg acttgtaat aacactcct 
      gctgataag aaaagcctg ttgcaggaa atcgtcgac 
      ttgtcaccc gtgttatca attctgctt ccatttatt 
      tatggaatc gccgttggt gtgctgata gcgtacatt 
      ctgagcgtg ttttcccga aaaaagcaa tcggatgag 
      cccgctcat gaccagaat ttgcgaaga agtggtacg 
      tttagccgg atgctcagt cgaggcgcg gtcactgag 
      gagcggcaa aacctgccc ctgcgcgag tggcacctc 
      tctgagtca acccccgta ctgtttagg aaaaaccag 
      tgctaa
    mRNA sequence
    • >TC004120 RA
      ataattaca ccagtgaaa tgaggtgtg tggcaacaa 
      aatgcagtt ttattcata cgttttttt atatttatt 
      gctgcccaa aactttgag taaacgagt cgaaattac 
      gtgcccgca acactgcaa gtgtgatat tttcgaaac 
      tttgagacg cgccacttg cacacgcgg gaatttggc 
      agggattga gtcaaatat accgacaca aacgcaaat 
      tctggacct atcctttaa ccggattag ctggcttca 
      gaaaaatgt gtttaagga ggaaaaact cacgaattt 
      gaaactact aaacctgtc ccacaacat agtgagtca 
      tattgaaat gaaggcttt caaaggctt gagtgcctt 
      gaggacgtt agatttgtc gtacaacag tgtccagta 
      tttaacccc agcctggtt tagggacat gacagcgct 
      ggaagaatt atatctgcg agggaacgg ctttagtaa 
      actggacag tggcccact tttcgcctc taagtcact 
      taaacgact tgacttgag cctgtgccg catatctta 
      cataggccc ggactcatt tagccagat cccgaatct 
      ggaagtctt ggacgtgtc tgagaacta tttgatcca 
      cctgtatat cgccacagt tgagccttt gacgaatct 
      ccaaatcct ggatgccgg taacaattc gttgtcctg 
      tgacggtgt ctcaaaaaa cttggcgaa atattgcgt 
      ccagaaggg aataaggtt caaggatcc gtgcaagcc 
      gacgatcgc cgattcgga aaagttcca gcgcatggt 
      taatttggc cgagccgga ggacgagaa aaattcatg 
      gatttatga cgacggcca agagatacg acatgacaa 
      tcaaacaac aacaaacgc gacttgtaa taacactcc 
      tgctgataa gaaaagcct gttgcagga aatcgtcga 
      cttgtcacc cgtgttatc aattctgct tccatttat 
      ttatggaat cgccgttgg tgtgctgat agcgtacat 
      tctgagcgt gttttcccg aaaaaagca atcggatga 
      gcccgctca tgaccagaa tttgcgaag aagtggtac 
      gtttagccg gatgctcag tcgaggcgc ggtcactga 
      ggagcggca aaacctgcc cctgcgcga gtggcacct 
      ctctgagtc aacccccgt actgtttag gaaaaacca 
      gtgctaatc tctctcaat aaaactgtg atactcctc 
      aaaatactt ttatttatc gctggttat gtacaaagt 
      ggaatgaat aaaatcaaa taaaacgg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY