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Gene information
General information
TC014273 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8266020..8269704
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014273
    iBeetle screen iB_10033

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014273 RA
      MADFGEDVV MDSEDLIIP KNPAELAEL KKENGNQLY 
      KVKQYRSAL NLYTEAIEL CPETAAYYS NRSACYMML 
      NNFQEALED ARKSITIDP AFSKGYIRI LKCAIALGD 
      LTTAENAIK QINLLDKNA NINNETRSF EKLRQFETE 
      AAKAMEKKD FRKVVYCMD RCLDEAPTC TRYKIMKAE 
      CLAFLGRYQ ESQEIANTA LHLDKTNAD AIYVRGMCL 
      YYEDNLDSA FNHFQQVLR LAPDHKKAM DIYKRAKEL 
      KKTKESGNE AYKNCRFQE AFTLYTEAL AIDPLNKKA 
      NAKLYFNRA TVQHRLTKT REAVDDCTS ALELDDTYL 
      KALLRRASC YMDLGEYED AVRDYEKVC KLNKSREHR 
      KLLQDAKFA LKKSKRKDY YKILGVERN ANDSEIKKA 
      YRKKALMLH PDRHANATD AVKKDQEKK FKELGEAYG 
      ILSDPKKKA RYDSGQDMD EFDGGMSDI DPTQVFQSF 
      FGQAHSCPN DFSFNTGFP FQFS
    CDS sequence
    • >TC014273 RA
      atggcagat tttggggaa gatgttgtt atggactct 
      gaagatttg ataattcca aaaaatcca gccgagctt 
      gccgaattg aaaaaagaa aatggcaat caattatac 
      aaagtcaag caataccga tctgccctc aatctctac 
      accgaagcc atcgaacta tgccccgaa accgccgcc 
      tattactcg aaccgctcg gcatgttac atgatgttg 
      aacaacttt caagaagca ttggaggat gcacgcaaa 
      tccataacc atcgatccc gcattcagc aaaggctac 
      attcgaata ttgaaatgc gctatagcc cttggcgac 
      cttacaaca gcagaaaat gccatcaaa cagatcaat 
      ctgcttgac aagaacgcc aacatcaac aatgaaact 
      cgttcgttc gaaaaactg agacagttc gaaacagag 
      gcagcaaaa gcaatggaa aagaaagac ttccgaaaa 
      gttgtttac tgcatggat aggtgtttg gatgaagcg 
      ccaacttgc acacgttac aaaataatg aaagccgaa 
      tgtttggct ttcttggga cgataccag gagtcgcaa 
      gaaatcgcc aacacggcc ttgcatttg gacaaaaca 
      aacgctgat gcgatttac gtacgggga atgtgtctt 
      tattacgaa gataatttg gacagcgcc tttaaccac 
      ttccagcaa gtcttgcga cttgcaccc gaccacaaa 
      aaggctatg gacatctac aagagagct aaagaactc 
      aaaaagacg aaggaaagt ggcaatgaa gcgtataaa 
      aactgccgg ttccaggaa gctttcact ctttatacc 
      gaagcacta gcaatcgac cctctcaac aaaaaggcc 
      aacgcgaaa ctttacttc aaccgtgcc acagtccaa 
      caccggctc accaaaacc agagaagcc gtcgatgac 
      tgcacttcg gcccttgaa ctcgacgac acgtatctc 
      aaggccctt ctgcgaagg gcgagctgc tacatggac 
      ttgggcgag tacgaggac gcagtccgg gattacgaa 
      aaagtgtgc aagttgaat aaaagtcgt gagcaccgc 
      aagttgctc caagatgcg aaattcgca ctcaaaaag 
      tccaaacgc aaggattat tacaagatt ttgggagtg 
      gagaggaac gcaaacgat agcgagatt aagaaagct 
      tacaggaag aaagcgttg atgttgcat ccggacagg 
      catgccaac gctacggac gccgttaaa aaagaccag 
      gagaagaag tttaaggag ctgggggag gcatacggg 
      attttgtca gatccgaag aaaaaggcc aggtatgat 
      agtgggcaa gacatggat gagttcgat gggggaatg 
      tcagatatt gacccgact caagtgttt cagtcgttc 
      tttggacaa gcgcacagt tgccccaat gatttcagt 
      tttaacacg ggatttcct ttccagttc agttag
    mRNA sequence
    • >TC014273 RA
      aacttgtgc aagatggca gattttggg gaagatgtt 
      gttatggac tctgaagat ttgataatt ccaaaaaat 
      ccagccgag cttgccgaa ttgaaaaaa gaaaatggc 
      aatcaatta tacaaagtc aagcaatac cgatctgcc 
      ctcaatctc tacaccgaa gccatcgaa ctatgcccc 
      gaaaccgcc gcctattac tcgaaccgc tcggcatgt 
      tacatgatg ttgaacaac tttcaagaa gcattggag 
      gatgcacgc aaatccata accatcgat cccgcattc 
      agcaaaggc tacattcga atattgaaa tgcgctata 
      gcccttggc gaccttaca acagcagaa aatgccatc 
      aaacagatc aatctgctt gacaagaac gccaacatc 
      aacaatgaa actcgttcg ttcgaaaaa ctgagacag 
      ttcgaaaca gaggcagca aaagcaatg gaaaagaaa 
      gacttccga aaagttgtt tactgcatg gataggtgt 
      ttggatgaa gcgccaact tgcacacgt tacaaaata 
      atgaaagcc gaatgtttg gctttcttg ggacgatac 
      caggagtcg caagaaatc gccaacacg gccttgcat 
      ttggacaaa acaaacgct gatgcgatt tacgtacgg 
      ggaatgtgt ctttattac gaagataat ttggacagc 
      gcctttaac cacttccag caagtcttg cgacttgca 
      cccgaccac aaaaaggct atggacatc tacaagaga 
      gctaaagaa ctcaaaaag acgaaggaa agtggcaat 
      gaagcgtat aaaaactgc cggttccag gaagctttc 
      actctttat accgaagca ctagcaatc gaccctctc 
      aacaaaaag gccaacgcg aaactttac ttcaaccgt 
      gccacagtc caacaccgg ctcaccaaa accagagaa 
      gccgtcgat gactgcact tcggccctt gaactcgac 
      gacacgtat ctcaaggcc cttctgcga agggcgagc 
      tgctacatg gacttgggc gagtacgag gacgcagtc 
      cgggattac gaaaaagtg tgcaagttg aataaaagt 
      cgtgagcac cgcaagttg ctccaagat gcgaaattc 
      gcactcaaa aagtccaaa cgcaaggat tattacaag 
      attttggga gtggagagg aacgcaaac gatagcgag 
      attaagaaa gcttacagg aagaaagcg ttgatgttg 
      catccggac aggcatgcc aacgctacg gacgccgtt 
      aaaaaagac caggagaag aagtttaag gagctgggg 
      gaggcatac gggattttg tcagatccg aagaaaaag 
      gccaggtat gatagtggg caagacatg gatgagttc 
      gatggggga atgtcagat attgacccg actcaagtg 
      tttcagtcg ttctttgga caagcgcac agttgcccc 
      aatgatttc agttttaac acgggattt cctttccag 
      ttcagttag gagcctggt gtactgtct aagtcataa 
      tctaaagcc cctcacatt ccactcctt cttgacagt 
      aaattgtgc tagatttaa caaatccta aagtttaac 
      ccgtttatt ttcctaatg cctcttctg tttatttat 
      tttgaggaa gactgcaca agttttctt gtttcatta 
      ttgtaaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY