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Gene information
General information
TC013543 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8247462..8251513
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC013543
    iBeetle screen iB_10032

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013543 RA
      MPLWASLLQ NTNFGVQIT TRRWNSTLR RIDKLLIAN 
      RGEIACRVL RTARRLGVK TVAVYSDAD KNSLHVSQA 
      DEAYNIGPP QASQSYLRG DKILEVAHK SGCQAIHPG 
      YGFLSENVE FAENCEKNK IIFMGPPAS AIRDMGIKS 
      KSKEIMSKA GVPIIGGYH GEDQDRAKL KAEALKIGF 
      PVMIKAVRG GGGKGMRIA LTESEFEEA LESAKSEAQ 
      KAFGDSVVL LEKFVQEPR HVEVQVFAD MHGNAVHLF 
      ERDCSVQRR HQKIIEEAP APGISEELR AELGAAAVR 
      AAKAVGYVG AGTVEFILD RKSHNFHFM EMNTRLQVE 
      HPITEMITG TDLVEWQIK VARGEKLPL TQEEITLSG 
      HAFESRIYA EDPSGGFLP GAGVLSYLS SPQVRDDMR 
      IETGIREGD EVSVHYDPM IAKLVVWGQ NRSESLLKM 
      QSLLLQYNV AGLETNVNF LLDLVRHPE FISGNVHTN 
      FIKENNDSL FKNKTVTPT QITQAALSV VLKDELLDM 
      KNAMGRNDQ FNPFVVESG FRINHNLIR DVKMKFKDE 
      EKLVKVKFI GHNTYHISL DEGKTWSVV QGEFNKESL 
      KSTIDGITT SVNVFRGDD VIAIFDDSG KVEFNLEKP 
      SFVTIQEDD SSAGGSLNR AVAPMPGVI DKVLVSAGD 
      QVKKGDSLF VLIAMKMEH VVKADRDAV IENIYFKVG 
      DNVQKDVTV IQFKEENVS S
    CDS sequence
    • >TC013543 RA
      atgccgttg tgggcctca ttgttacaa aacacaaat 
      ttcggcgtt caaataaca acaagaaga tggaacagt 
      actttgaga aggatagat aaattatta attgcaaat 
      agaggtgag atagcttgt cgtgtgttg aggactgcc 
      cgccggttg ggcgtaaaa acggtcgcg gtttatagc 
      gacgctgat aagaattcg ttacacgtt tcacaggca 
      gatgaggcc tataacatc ggccctccc caagcctcg 
      caaagttac ctccgaggt gacaaaatt ttagaagta 
      gcgcacaaa tccggctgt caggcgatc catccaggc 
      tacggcttc ctttccgaa aatgtggaa tttgcggaa 
      aattgcgaa aaaaacaaa atcattttc atgggccct 
      ccggcttcg gcgattcgc gacatggga atcaaaagc 
      aaatcgaag gaaattatg tcgaaagcc ggggttccc 
      ataatcggg ggttaccac ggggaagac caagacagg 
      gccaagctc aaagcagaa gccctgaaa atcggattt 
      cccgtcatg ataaaagcc gtgagaggt ggtggcggc 
      aaaggaatg aggatagct ctcacggag tctgaattt 
      gaggaagcc ttggagtcg gccaaaagt gaggcgcag 
      aaagctttc ggcgattct gtggtgctt ttggagaaa 
      ttcgtgcaa gagccgagg cacgtggaa gtgcaggtt 
      tttgcagat atgcatggc aatgctgtt cacttgttc 
      gaacgggat tgttcggtg cagaggagg catcagaaa 
      attatagag gaagcgcca gcgccggga ataagcgaa 
      gaattgagg gctgaactg ggggctgct gccgtaaga 
      gctgccaaa gcggttggg tatgtgggg gccgggacg 
      gtggaattt attctagac aggaaaagt cacaatttt 
      cattttatg gaaatgaat acgcgattg caggtagaa 
      cacccgata acggagatg attacagga accgatttg 
      gtggaatgg caaataaaa gtcgctcga ggtgaaaag 
      ttgccatta actcaggag gaaataaca ctgagtggc 
      cacgcgttt gagtctcga atttacgcg gaggaccca 
      agtgggggc tttttgcca ggggctggt gttttgtcg 
      tatttgagt agtccccaa gttcgggat gacatgagg 
      attgaaact ggcattcga gaaggggat gaagtgtcg 
      gttcattac gaccccatg attgccaaa ctggtcgtt 
      tggggccaa aacagatct gaaagtctc ctaaaaatg 
      caatccctt ctcttgcag tataacgtg gcaggtttg 
      gaaaccaac gttaatttc cttctagat cttgtaagg 
      catccggaa tttatttcc ggaaacgtt cacactaat 
      ttcattaaa gaaaacaat gatagttta tttaaaaat 
      aaaaccgta actccaacg caaatcact caagcagct 
      ttaagcgtg gttttaaaa gacgagtta ttagacatg 
      aaaaatgcc atgggtcga aatgaccaa tttaaccca 
      tttgtggtt gaatctggg ttccgaatt aaccacaac 
      ttgattcgc gacgtcaaa atgaaattt aaggatgaa 
      gaaaaacta gttaaagta aaatttatt ggccacaac 
      acgtaccac atttcgctc gatgaggga aagacctgg 
      agtgtagtt cagggcgaa tttaacaaa gaaagtcta 
      aaaagcaca attgatggt atcaccacc agtgtgaat 
      gttttcagg ggtgacgac gttattgca attttcgat 
      gatagtggt aaagttgaa tttaattta gaaaagcca 
      tcgtttgtg acaattcaa gaagatgat tcttcggca 
      ggaggttcg ttgaacaga gctgttgcc ccaatgccc 
      ggtgtcata gacaaggtc ttggtcagt gctggagac 
      caagtgaaa aaaggagat tcgcttttt gtcttaata 
      gctatgaaa atggaacat gtggtgaag gccgatcgg 
      gacgctgtt attgaaaat atttatttc aaagtcggt 
      gataacgtc cagaaggat gttactgtt atacaattt 
      aaggaggaa aatgtgtct agctaa
    mRNA sequence
    • >TC013543 RA
      cagcatcat tatgataaa aatcgcatt ttagttaat 
      agtgttaac aaaactgtg ccattaaaa tcaagataa 
      aaatgccgt tgtgggcct cattgttac aaaacacaa 
      atttcggcg ttcaaataa caacaagaa gatggaaca 
      gtactttga gaaggatag ataaattat taattgcaa 
      atagaggtg agatagctt gtcgtgtgt tgaggactg 
      cccgccggt tgggcgtaa aaacggtcg cggtttata 
      gcgacgctg ataagaatt cgttacacg tttcacagg 
      cagatgagg cctataaca tcggccctc cccaagcct 
      cgcaaagtt acctccgag gtgacaaaa ttttagaag 
      tagcgcaca aatccggct gtcaggcga tccatccag 
      gctacggct tcctttccg aaaatgtgg aatttgcgg 
      aaaattgcg aaaaaaaca aaatcattt tcatgggcc 
      ctccggctt cggcgattc gcgacatgg gaatcaaaa 
      gcaaatcga aggaaatta tgtcgaaag ccggggttc 
      ccataatcg ggggttacc acggggaag accaagaca 
      gggccaagc tcaaagcag aagccctga aaatcggat 
      ttcccgtca tgataaaag ccgtgagag gtggtggcg 
      gcaaaggaa tgaggatag ctctcacgg agtctgaat 
      ttgaggaag ccttggagt cggccaaaa gtgaggcgc 
      agaaagctt tcggcgatt ctgtggtgc ttttggaga 
      aattcgtgc aagagccga ggcacgtgg aagtgcagg 
      tttttgcag atatgcatg gcaatgctg ttcacttgt 
      tcgaacggg attgttcgg tgcagagga ggcatcaga 
      aaattatag aggaagcgc cagcgccgg gaataagcg 
      aagaattga gggctgaac tgggggctg ctgccgtaa 
      gagctgcca aagcggttg ggtatgtgg gggccggga 
      cggtggaat ttattctag acaggaaaa gtcacaatt 
      ttcatttta tggaaatga atacgcgat tgcaggtag 
      aacacccga taacggaga tgattacag gaaccgatt 
      tggtggaat ggcaaataa aagtcgctc gaggtgaaa 
      agttgccat taactcagg aggaaataa cactgagtg 
      gccacgcgt ttgagtctc gaatttacg cggaggacc 
      caagtgggg gctttttgc caggggctg gtgttttgt 
      cgtatttga gtagtcccc aagttcggg atgacatga 
      ggattgaaa ctggcattc gagaagggg atgaagtgt 
      cggttcatt acgacccca tgattgcca aactggtcg 
      tttggggcc aaaacagat ctgaaagtc tcctaaaaa 
      tgcaatccc ttctcttgc agtataacg tggcaggtt 
      tggaaacca acgttaatt tccttctag atcttgtaa 
      ggcatccgg aatttattt ccggaaacg ttcacacta 
      atttcatta aagaaaaca atgatagtt tatttaaaa 
      ataaaaccg taactccaa cgcaaatca ctcaagcag 
      ctttaagcg tggttttaa aagacgagt tattagaca 
      tgaaaaatg ccatgggtc gaaatgacc aatttaacc 
      catttgtgg ttgaatctg ggttccgaa ttaaccaca 
      acttgattc gcgacgtca aaatgaaat ttaaggatg 
      aagaaaaac tagttaaag taaaattta ttggccaca 
      acacgtacc acatttcgc tcgatgagg gaaagacct 
      ggagtgtag ttcagggcg aatttaaca aagaaagtc 
      taaaaagca caattgatg gtatcacca ccagtgtga 
      atgttttca ggggtgacg acgttattg caattttcg 
      atgatagtg gtaaagttg aatttaatt tagaaaagc 
      catcgtttg tgacaattc aagaagatg attcttcgg 
      caggaggtt cgttgaaca gagctgttg ccccaatgc 
      ccggtgtca tagacaagg tcttggtca gtgctggag 
      accaagtga aaaaaggag attcgcttt ttgtcttaa 
      tagctatga aaatggaac atgtggtga aggccgatc 
      gggacgctg ttattgaaa atatttatt tcaaagtcg 
      gtgataacg tccagaagg atgttactg ttatacaat 
      ttaaggagg aaaatgtgt ctagctaaa aattttgaa 
      taaattttg agttctact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY