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Gene information
General information
TC013552 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8128400..8130087
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC013552
    iBeetle screen iB_10030

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
     labrum size decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    labrum mostlynot present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    antenna slightly orientation irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC013552 RA
      MYFLLFNYL FFVIVHFCN ANEACLKEN SEQCNASKV 
NMYSKEANT KYLKYLDLI KRAKENYSP CDNTKCGCY 
SSQISDDLK IFKKGITPQ LIDKVKTKG TKYQIIDHK 
LYRDKNCMF PARCAGIEH FLLKLLPKL PDMELIINT 
RDWPQIHKD YGVFGPVFS FSKTSDYSD IMYPAWAFW 
EGGPAISLY PRGIGRWDT HRDLLGKKG NETLWDEKI 
PKGFFRGSR TSAERDPLV LLSREKPHL VDAQYTKNQ 
AWKSDADTL HQPPAPEVS FEDHCKYKY LFNFRGVAA 
SFRFKHILL CKSLVFHVG SDWLEFFYP ALKPWIHYI 
PVEANASKE KIEELVQFV LSYDNIAKE IAENGYNMI 
WNNLKLVDV TCYWRKLLK QYAKLLTYK PEIDNDLIE 
IKK
    CDS sequence
    • >TC013552 RA
      atgtatttc cttttgttt aattatctg tttttcgta 
attgtgcac ttttgcaat gcaaacgaa gcgtgtttg 
aaggagaac agtgaacaa tgcaacgct tcgaaagtt 
aacatgtac tcaaaagaa gccaacacc aaatattta 
aagtacctt gatttgatt aaaagagcc aaggagaac 
tattcacct tgtgacaac acaaaatgt gggtgctat 
tcctcacaa atatcagac gatttgaag atatttaaa 
aagggcata acgccacaa ttaattgac aaagttaaa 
acaaaagga actaaatac caaataatt gatcacaaa 
ttatacaga gacaagaac tgcatgttc ccggcccga 
tgtgccggc attgagcat tttttactg aaactttta 
cccaaacta ccagacatg gaactcata attaacacc 
agagactgg ccccaaatc cataaagac tacggcgtg 
ttcgggcct gtgttttcc ttcagcaag acaagcgat 
tatagcgat ataatgtac ccagcgtgg gcattctgg 
gaagggggc ccagctata tccctgtac ccgagagga 
attggacgg tgggataca catcgtgat ttgttgggc 
aaaaagggc aacgaaact ctctgggac gaaaaaatc 
ccgaagggg tttttccgg ggctcgcgc actagcgcc 
gagcgggac cctttggtg ctcctgtcg agggaaaaa 
ccccacttg gttgatgca caatacacc aaaaaccag 
gcctggaaa tcggacgcg gacactttg catcagcct 
ccggcgcct gaagtgtct tttgaggac cactgcaaa 
tacaagtat ctttttaat ttcaggggt gtggctgcc 
agttttcgt tttaagcac attctcttg tgtaaatct 
ctcgttttt catgttggg agtgattgg ttggagttt 
ttctatccg gctttgaag ccttggatt cattacatt 
ccagtggag gcgaatgcg agtaaagag aaaattgag 
gaattggta cagtttgta ttgagttat gataatatt 
gctaaagag attgcggaa aatgggtat aatatgatt 
tggaataat ctaaagttg gttgatgta acttgctac 
tggagaaag ttgcttaaa caatatgct aagttgttg 
acttacaaa cccgaaatt gataacgat ctgatagaa 
attaaaaaa taa
    mRNA sequence
    • >TC013552 RA
      cgtgacgtg ttaccctaa cctcaacaa acaaaaatg 
tatttcctt ttgtttaat tatctgttt ttcgtaatt 
gtgcacttt tgcaatgca aacgaagcg tgtttgaag 
gagaacagt gaacaatgc aacgcttcg aaagttaac 
atgtactca aaagaagcc aacaccaaa tatttaaag 
taccttgat ttgattaaa agagccaag gagaactat 
tcaccttgt gacaacaca aaatgtggg tgctattcc 
tcacaaata tcagacgat ttgaagata tttaaaaag 
ggcataacg ccacaatta attgacaaa gttaaaaca 
aaaggaact aaataccaa ataattgat cacaaatta 
tacagagac aagaactgc atgttcccg gcccgatgt 
gccggcatt gagcatttt ttactgaaa cttttaccc 
aaactacca gacatggaa ctcataatt aacaccaga 
gactggccc caaatccat aaagactac ggcgtgttc 
gggcctgtg ttttccttc agcaagaca agcgattat 
agcgatata atgtaccca gcgtgggca ttctgggaa 
gggggccca gctatatcc ctgtacccg agaggaatt 
ggacggtgg gatacacat cgtgatttg ttgggcaaa 
aagggcaac gaaactctc tgggacgaa aaaatcccg 
aaggggttt ttccggggc tcgcgcact agcgccgag 
cgggaccct ttggtgctc ctgtcgagg gaaaaaccc 
cacttggtt gatgcacaa tacaccaaa aaccaggcc 
tggaaatcg gacgcggac actttgcat cagcctccg 
gcgcctgaa gtgtctttt gaggaccac tgcaaatac 
aagtatctt tttaatttc aggggtgtg gctgccagt 
tttcgtttt aagcacatt ctcttgtgt aaatctctc 
gtttttcat gttgggagt gattggttg gagtttttc 
tatccggct ttgaagcct tggattcat tacattcca 
gtggaggcg aatgcgagt aaagagaaa attgaggaa 
ttggtacag tttgtattg agttatgat aatattgct 
aaagagatt gcggaaaat gggtataat atgatttgg 
aataatcta aagttggtt gatgtaact tgctactgg 
agaaagttg cttaaacaa tatgctaag ttgttgact 
tacaaaccc gaaattgat aacgatctg atagaaatt 
aaaaaataa aaaggaact caacgatct aaactaatg 
acaataata ctgaaatga caataatta gtcttaagg 
aacctactt actatattt aaatgtgat atttttttt 
gtatttatt ttattattc ttatcatgt tgttttata 
ttttatcta ttcctatat cgtagttgg aaatgttag 
tctattaaa aatatagca caacaaata tg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY