Status
Please wait ...
Gene information
General information
TC014256 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 8007099..8075667
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC014256
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_10027

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC014256 RA
      MSALATGVN KKRKKSETK PQAQINKCN NEKRRREQE 
      NIYIEELAE LISANFADM SSLSVKPDK CAILQETVN 
      QIRSIKQRE SASQSSDPV QQGEVSSSR PTILSNEVY 
      GPLLLEALE GFLFVVNAE GKVEHVTEN VSNYIKFTR 
      DEIFGNSIY NFIHLGDHA RFTTSLMPM IGWGSESTT 
      TTRSRSFSV RFLVKPPDD QDETVEEKQ QRVNCYELM 
      HISSTQLRD QVSVSDDDG ADSGPYLLC VASRISHRD 
      KVISGIEQF TTKLDTSGK IIGVDASGV SAPYSQFIN 
      KELMDRALR DLVFQQDVH KLNTHLKET IHTGQATSA 
      VYRLQLGQD KYVQVQTKS KLFKTNPHN SNDIDFIMA 
      THSIVGEYD ATGPSDPGG GGTSSVGGP LMTSVVNGT 
      RNGTAPVSS GSDSSTASS NALMNAGNA SFTPYIDND 
      FPFDIFPTS TFELEPSGW TEARPDSRQ SATPVSTPT 
      PRPPSNPAY SPATTVAQS PLAPFVTQP SPSTPAAPN 
      PYTSTFSFS PLSEQNYNV EEPKDTKAN VLDEASSMM 
      ADSARLRNL LTKPPTVAD SSANDADGR NKNRILKGL 
      LNQQDEDDN RNDNRASPR GGLISRGAV AGPSELPKT 
      STAGGNNML LQLLNERSD DDDNLEARA GVRKKSELL 
      HQLLKTESR ENEDEKKND VQSHDDSLL RSLGFTSSP 
      SPPSGEPGR SRKRPSDDR DENVSSKRT SDGSQVSST 
      GAGTGSKLC EKNKMLASL LAKTPNNQQ PIPHIPVSL 
      MSATPQDIL PRISSLPET VKTSKPMPT RSTTSVTQQ 
      RTVVKNTLP DNMSRMGAR QPTTNYLTT MLTHPNSMH 
      HQRTPDNRQ MAQVDSGSY TSPATSSTD GNIDASMWV 
      DNNNSNDPL LSDILDQVM DIVPDEVGR TFSAGEGQQ 
      PNNFHPNEL SEKMAINII QKSLMQCES VVKSPSSPT 
      ITLPGTPPA YTPAAMTTQ NSQMRPFQP PPNYNQASF 
      IAKQRLAVR PGAPQYAVA TGGLTNSQQ LQIQRRQQE 
      EKRRLLQQQ QQQELLIPS NATAAEINS GLQNIDSLL 
      NNTVAPNVS LQRSASLPE SQLSPNYGG QLNQPNQRI 
      NSQQPYSPH SQLVSPIGQ QAGFPQTSA ANYQQAGAR 
      LSPQFTQQM ALRQAYPQG SAQGQNWQQ NQARLSLQN 
      PMLNAQLTG NYVSGRNFQ RAPNQPSAA QQQQQQQQQ 
      QRSLNSPGT VTSRHSPYQ QDSFPPPSS PNSTAFNQT 
      QYLRLQRAN SVPTATTQL PGGLGSPRP YGREHHPHP 
      YPPIPPNPH QHPMMYQQD SSQYCYDQT GLQLAYNGA 
      DRGRAPPHL QAGVSASGP TSEFVRQEL RAVVGARTG 
      QAQTAPNRT QSQLLNQQQ VDLEALGIT FEMPTSEFY 
      GGGAGR
    CDS sequence
    • >TC014256 RA
      atgagcgcc ttggccacc ggtgtcaat aagaaaagg 
      aaaaaatcc gaaacaaaa cctcaggcg cagattaac 
      aaatgcaac aatgaaaaa cggcgccgc gagcaggaa 
      aacatttac atagaagag ctggctgag ctaatatcc 
      gcaaacttc gcggacatg agctcgttg tcggtaaag 
      ccggacaag tgcgctatc cttcaagag accgtcaac 
      cagatccgg agcatcaag cagcgggag tcggcgtcg 
      cagtcctcc gatcccgtc cagcaggga gaggtgtcg 
      tcgtcgcgg ccgaccatc ctctcgaac gaggtgtac 
      ggccccctc ctcctggag gccctggaa ggcttcctg 
      ttcgtggtg aacgccgag ggcaaggtc gagcacgtc 
      accgagaac gtctccaac tacatcaag ttcacccgc 
      gacgagatc ttcggcaac agcatctac aacttcatc 
      catctgggc gaccacgcg cgcttcacc acctcgttg 
      atgccgatg atcggctgg ggcagcgag tcgacgacg 
      acgacgcga tcgcgctcc ttcagcgtg cgcttcctg 
      gtgaagccg cccgacgac caggacgag accgtggag 
      gagaagcag cagcgggtc aactgctat gagctgatg 
      cacatctcc agcacgcag ctgagagac caagtgtcg 
      gtgtcggac gacgacggt gccgacagc ggaccgtat 
      ttgttgtgc gtggcgagc aggatttcg cacagggac 
      aaagttatt tccgggatt gaacagttc acgaccaag 
      ttggacacc tccggcaag atcatcggg gtcgatgcg 
      tcgggggtg tcggcgccg tattcgcaa ttcattaac 
      aaagaactt atggatcgc gcgctcagg gatctggtg 
      ttccagcag gacgtgcac aaacttaac acgcatctc 
      aaagaaacc attcatacg ggacaagcg actagtgcc 
      gtttatagg ttacaactc gggcaggac aagtacgtg 
      caagtgcaa accaaatcg aaattgttc aagactaat 
      ccgcataac agcaacgat attgacttt atcatggcc 
      acgcattcg attgtgggc gagtacgac gcaaccggt 
      ccttcggat cccggcggc ggaggaacc agcagcgtc 
      ggcggcccg ctgatgacg agcgtggtg aacggcacc 
      cgcaatggc accgccccc gtcagctct ggcagcgac 
      agctcgacg gcctcctcg aacgcgctg atgaacgcc 
      ggcaacgcg tccttcacc ccctacatc gacaacgac 
      ttccccttc gacatcttt ccgacgtcg acgttcgag 
      ctggagccg tccggctgg acggaagcg cggcccgac 
      tcccggcaa agcgcgacg cccgtctcg acgccgacg 
      ccccgacct cccagcaat cctgcctac agcccggcc 
      acgacggtg gctcagagc ccgctcgct cccttcgtc 
      acgcagccg agtccgtcg acgcccgcc gcccccaac 
      ccttacact agtaccttc tcatttagc ccgctgtcc 
      gagcagaac tacaatgtc gaagaacca aaagatacc 
      aaagcaaac gtcttggat gaggcctcc agtatgatg 
      gccgattcc gctagatta aggaatttg ttaactaag 
      ccgcccacg gtcgccgat tcctcggcg aacgacgcg 
      gacggcagg aacaaaaat aggatattg aaaggactg 
      ctgaatcag caggacgaa gacgataat aggaacgat 
      aacagggcc agtcccaga ggcgggctc atcagtcga 
      ggcgcggtc gcaggacct tcagagtta ccgaaaacc 
      tccacagct ggaggaaac aacatgctg ctacagttg 
      ctgaatgag agaagcgac gacgacgat aatctcgaa 
      gcgagagct ggcgtaagg aagaaaagt gagttactt 
      catcagctg ctcaaaact gaaagtcgc gaaaacgaa 
      gacgaaaag aaaaacgat gttcaatcg cacgacgac 
      tcgttattg cgtagtttg ggcttcacc agctcgcct 
      tcgcctccc agtggagag ccgggcagg agtcggaaa 
      aggcccagt gacgacagg gacgaaaac gtctccagc 
      aaaagaaca tcggatgga tctcaggtt tcgtcgacg 
      ggcgccgga acgggaagc aagctgtgt gagaagaac 
      aaaatgctg gcctccctg ttggcgaaa acgcccaac 
      aaccagcaa cccatcccc catattcca gtaagcctt 
      atgtcggcg acaccgcaa gatatttta ccccggatc 
      tcatcacta cccgaaacg gtgaaaacg agcaagccc 
      atgccgacg cgatctact acctctgtc acgcaacaa 
      cggaccgtt gtcaagaac acactgccg gacaacatg 
      tcgcgaatg ggcgcccga caaccgacc accaactac 
      ctcacaacc atgttgacg cacccgaac agcatgcac 
      caccagcga acgcccgat aaccggcag atggctcaa 
      gtggattct ggttcctac acctcgccg gctacgtcc 
      agcaccgac ggaaacatc gacgcctcc atgtgggtc 
      gacaacaac aactccaac gatcccctg ctgtcggac 
      atcctggac caggtcatg gacatcgtg cccgacgag 
      gtgggccgg acgttttcg gcaggcgag ggccaacag 
      ccgaacaat ttccacccg aatgaactc tcggagaag 
      atggccatt aacattatc caaaagtcg ctaatgcaa 
      tgcgaatcg gttgtcaag agcccttcg tcgcctacg 
      atcacactg ccggggacg ccgcctgcg tacactcca 
      gctgcaatg acgacccag aacagccaa atgcggccg 
      ttccagccc cctcccaac tacaaccag gcgagcttt 
      atagccaag cagcgcctc gccgtgcgc cctggcgcc 
      ccccaatac gctgtcgca accggtggc ctcaccaac 
      agccagcag ttgcaaatc cagcgtcga cagcaggag 
      gaaaaacgc cgccttctc caacagcaa cagcagcag 
      gaactttta attccgtcc aacgcaacc gcagctgaa 
      ataaactcg gggttacag aacattgat agcctcctc 
      aacaataca gtagcaccc aatgtatcc cttcaacga 
      tcggccagt ttaccagaa tctcaactc tctcccaat 
      tatggagga cagttgaat caaccgaat cagagaatc 
      aacagtcaa cagccatat tcacctcat tcgcaattg 
      gtgtcgccg attggacaa caagccggg tttccgcag 
      acttcagcc gccaactac caacaagct ggggcgaga 
      ctgtcgcca cagttcacg caacagatg gcgctgagg 
      caggcgtat ccgcaggga agtgcgcaa ggacagaac 
      tggcagcag aaccaggcc aggctcagt ctgcagaac 
      cccatgctc aatgcccag ctcaccggc aactatgta 
      tcaggacga aattttcag agagcaccg aaccagccg 
      tcggcggcg caacagcag cagcaacaa cagcagcag 
      cagaggtct ctgaacagt cctggaacg gtgacttcg 
      cggcactcg ccgtatcag caagactcg tttcctcca 
      ccttcatcc cccaactct accgctttc aaccagact 
      cagtacctg aggctacag cgcgctaac agcgtgccc 
      actgccaca acccagttg ccaggtggc ctgggatcc 
      ccccggcca tacggcagg gaacaccac ccccatccc 
      tatcccccg attcccccc aacccccac caacacccc 
      atgatgtac caacaagac tcctcccag tactgttat 
      gatcagacg ggtttacag ttagcctac aatggcgca 
      gacaggggt cgagcgccc ccacacctc caagccggt 
      gtgtccgca agtggcccc acatcggag ttcgtccgg 
      caagagtta cgtgcggtg gtcggtgcc agaactggg 
      caagcacag acagcccca aataggact cagtctcaa 
      ttactcaac cagcaacaa gttgatctc gaagcctta 
      ggaattacg ttcgaaatg cccacgtct gagttctat 
      ggaggaggc gcgggcagg tga
    mRNA sequence
    • >TC014256 RA
      ttgttgtca agcacagga gcgcatgga gattagggt 
      gtctcaggc aacacgatt ccctttgca attctttat 
      caatacgaa aatcgcaat tttaccatg taaaaatca 
      aatcgcaga ggcgttatt caacgattt aacaaaact 
      ctagtgtgt tttttaaca taatatggt aatggtttt 
      tagtgacaa tgtcggttt tttgagaaa aaacaaatt 
      ttatcgtca tgtatgaat tttttatgc catcggaat 
      ggacacctt cagttcgaa ctaccttta ttatgggga 
      aaatgacga caacagaac ggaacaaag gtgagtcca 
      atttattaa ttacgacaa tttgagtgt aaaaaattt 
      tgatgattt ttgtgacgg aatttaaat ttgcgccct 
      ctcgggtta gtgacactt gaataaact gtgttcgtg 
      gaactaaat tgtaaattt aaacatttt aaaaggttt 
      ttaatgaga ctgatagcc aacatatca attatcaac 
      cgagtaaac attacgtcg gcgtaatga tttaagaca 
      acgtttggc tgaagctac aagtttacc taattcaat 
      aaaattatg tcagtgccc gttttttgg attttaaaa 
      aaagcacaa tttgaaaaa tttgatttg aatggggtt 
      cagtaaagt ggcgccgcc gctggtcac ttgcccaag 
      ctgccacag ctcgcttga tgtttgctt tcgagttgg 
      atagagagc tctactttg aaaatgcat tatagaagt 
      tacatcccg caatgaaat aattgtggc acttactgg 
      acgcacttg ggattccgt ttcagtttc actcgtttt 
      aaaataaaa tttgggaat gaattcaag ctgacaggt 
      gcatgtcaa tgttttgtc cataacctt gaacgcggc 
      caatcaggg cccgcgccg cgttccttc gaccaatca 
      gatttgtcg tagagccag ggtcaacaa atccttgtg 
      aaatagccg agttttgtg tcagccatg ttgaaaaca 
      accttttct tcgacgtgt gctattttt tggtatttt 
      cacgtgttt atgtgcaat aaaggcgaa taacagttg 
      tccaggtgt tgtgtttcg gttttcggt cagttattt 
      gtgcagtgc gaagatccc gatgggact ttttcacgg 
      cggtttacg gtgttttca tgtaacgag accttgaat 
      agtgtttgg cttccaagc aaacttttc atagttttg 
      gatacggct cagtatgat tttttacga aacagactc 
      ggtccgtgt gagttgcag gacccggcg tgggccaag 
      atgagcgcc ttggccacc ggtgtcaat aagaaaagg 
      aaaaaatcc gaaacaaaa cctcaggcg cagattaac 
      aaatgcaac aatgaaaaa cggcgccgc gagcaggaa 
      aacatttac atagaagag ctggctgag ctaatatcc 
      gcaaacttc gcggacatg agctcgttg tcggtaaag 
      ccggacaag tgcgctatc cttcaagag accgtcaac 
      cagatccgg agcatcaag cagcgggag tcggcgtcg 
      cagtcctcc gatcccgtc cagcaggga gaggtgtcg 
      tcgtcgcgg ccgaccatc ctctcgaac gaggtgtac 
      ggccccctc ctcctggag gccctggaa ggcttcctg 
      ttcgtggtg aacgccgag ggcaaggtc gagcacgtc 
      accgagaac gtctccaac tacatcaag ttcacccgc 
      gacgagatc ttcggcaac agcatctac aacttcatc 
      catctgggc gaccacgcg cgcttcacc acctcgttg 
      atgccgatg atcggctgg ggcagcgag tcgacgacg 
      acgacgcga tcgcgctcc ttcagcgtg cgcttcctg 
      gtgaagccg cccgacgac caggacgag accgtggag 
      gagaagcag cagcgggtc aactgctat gagctgatg 
      cacatctcc agcacgcag ctgagagac caagtgtcg 
      gtgtcggac gacgacggt gccgacagc ggaccgtat 
      ttgttgtgc gtggcgagc aggatttcg cacagggac 
      aaagttatt tccgggatt gaacagttc acgaccaag 
      ttggacacc tccggcaag atcatcggg gtcgatgcg 
      tcgggggtg tcggcgccg tattcgcaa ttcattaac 
      aaagaactt atggatcgc gcgctcagg gatctggtg 
      ttccagcag gacgtgcac aaacttaac acgcatctc 
      aaagaaacc attcatacg ggacaagcg actagtgcc 
      gtttatagg ttacaactc gggcaggac aagtacgtg 
      caagtgcaa accaaatcg aaattgttc aagactaat 
      ccgcataac agcaacgat attgacttt atcatggcc 
      acgcattcg attgtgggc gagtacgac gcaaccggt 
      ccttcggat cccggcggc ggaggaacc agcagcgtc 
      ggcggcccg ctgatgacg agcgtggtg aacggcacc 
      cgcaatggc accgccccc gtcagctct ggcagcgac 
      agctcgacg gcctcctcg aacgcgctg atgaacgcc 
      ggcaacgcg tccttcacc ccctacatc gacaacgac 
      ttccccttc gacatcttt ccgacgtcg acgttcgag 
      ctggagccg tccggctgg acggaagcg cggcccgac 
      tcccggcaa agcgcgacg cccgtctcg acgccgacg 
      ccccgacct cccagcaat cctgcctac agcccggcc 
      acgacggtg gctcagagc ccgctcgct cccttcgtc 
      acgcagccg agtccgtcg acgcccgcc gcccccaac 
      ccttacact agtaccttc tcatttagc ccgctgtcc 
      gagcagaac tacaatgtc gaagaacca aaagatacc 
      aaagcaaac gtcttggat gaggcctcc agtatgatg 
      gccgattcc gctagatta aggaatttg ttaactaag 
      ccgcccacg gtcgccgat tcctcggcg aacgacgcg 
      gacggcagg aacaaaaat aggatattg aaaggactg 
      ctgaatcag caggacgaa gacgataat aggaacgat 
      aacagggcc agtcccaga ggcgggctc atcagtcga 
      ggcgcggtc gcaggacct tcagagtta ccgaaaacc 
      tccacagct ggaggaaac aacatgctg ctacagttg 
      ctgaatgag agaagcgac gacgacgat aatctcgaa 
      gcgagagct ggcgtaagg aagaaaagt gagttactt 
      catcagctg ctcaaaact gaaagtcgc gaaaacgaa 
      gacgaaaag aaaaacgat gttcaatcg cacgacgac 
      tcgttattg cgtagtttg ggcttcacc agctcgcct 
      tcgcctccc agtggagag ccgggcagg agtcggaaa 
      aggcccagt gacgacagg gacgaaaac gtctccagc 
      aaaagaaca tcggatgga tctcaggtt tcgtcgacg 
      ggcgccgga acgggaagc aagctgtgt gagaagaac 
      aaaatgctg gcctccctg ttggcgaaa acgcccaac 
      aaccagcaa cccatcccc catattcca gtaagcctt 
      atgtcggcg acaccgcaa gatatttta ccccggatc 
      tcatcacta cccgaaacg gtgaaaacg agcaagccc 
      atgccgacg cgatctact acctctgtc acgcaacaa 
      cggaccgtt gtcaagaac acactgccg gacaacatg 
      tcgcgaatg ggcgcccga caaccgacc accaactac 
      ctcacaacc atgttgacg cacccgaac agcatgcac 
      caccagcga acgcccgat aaccggcag atggctcaa 
      gtggattct ggttcctac acctcgccg gctacgtcc 
      agcaccgac ggaaacatc gacgcctcc atgtgggtc 
      gacaacaac aactccaac gatcccctg ctgtcggac 
      atcctggac caggtcatg gacatcgtg cccgacgag 
      gtgggccgg acgttttcg gcaggcgag ggccaacag 
      ccgaacaat ttccacccg aatgaactc tcggagaag 
      atggccatt aacattatc caaaagtcg ctaatgcaa 
      tgcgaatcg gttgtcaag agcccttcg tcgcctacg 
      atcacactg ccggggacg ccgcctgcg tacactcca 
      gctgcaatg acgacccag aacagccaa atgcggccg 
      ttccagccc cctcccaac tacaaccag gcgagcttt 
      atagccaag cagcgcctc gccgtgcgc cctggcgcc 
      ccccaatac gctgtcgca accggtggc ctcaccaac 
      agccagcag ttgcaaatc cagcgtcga cagcaggag 
      gaaaaacgc cgccttctc caacagcaa cagcagcag 
      gaactttta attccgtcc aacgcaacc gcagctgaa 
      ataaactcg gggttacag aacattgat agcctcctc 
      aacaataca gtagcaccc aatgtatcc cttcaacga 
      tcggccagt ttaccagaa tctcaactc tctcccaat 
      tatggagga cagttgaat caaccgaat cagagaatc 
      aacagtcaa cagccatat tcacctcat tcgcaattg 
      gtgtcgccg attggacaa caagccggg tttccgcag 
      acttcagcc gccaactac caacaagct ggggcgaga 
      ctgtcgcca cagttcacg caacagatg gcgctgagg 
      caggcgtat ccgcaggga agtgcgcaa ggacagaac 
      tggcagcag aaccaggcc aggctcagt ctgcagaac 
      cccatgctc aatgcccag ctcaccggc aactatgta 
      tcaggacga aattttcag agagcaccg aaccagccg 
      tcggcggcg caacagcag cagcaacaa cagcagcag 
      cagaggtct ctgaacagt cctggaacg gtgacttcg 
      cggcactcg ccgtatcag caagactcg tttcctcca 
      ccttcatcc cccaactct accgctttc aaccagact 
      cagtacctg aggctacag cgcgctaac agcgtgccc 
      actgccaca acccagttg ccaggtggc ctgggatcc 
      ccccggcca tacggcagg gaacaccac ccccatccc 
      tatcccccg attcccccc aacccccac caacacccc 
      atgatgtac caacaagac tcctcccag tactgttat 
      gatcagacg ggtttacag ttagcctac aatggcgca 
      gacaggggt cgagcgccc ccacacctc caagccggt 
      gtgtccgca agtggcccc acatcggag ttcgtccgg 
      caagagtta cgtgcggtg gtcggtgcc agaactggg 
      caagcacag acagcccca aataggact cagtctcaa 
      ttactcaac cagcaacaa gttgatctc gaagcctta 
      ggaattacg ttcgaaatg cccacgtct gagttctat 
      ggaggaggc gcgggcagg tgatcacgg taacaagtc 
      gtcgtcgct gctgcagaa gctgctgtc cgagtgacc 
      accgcgtgt acataatag agatatata ctactaggt 
      acctcatcg gttcagtac cgcaggctg taatttaga 
      caggttagc tgtatcaag tgcattact acgttgctt 
      gttcgtttc gttagggta gtgcggagc gagggaagg 
      cgcagaggg cttgagtgc aattgtaaa taaatagga 
      ggactttgt ttatattta tatatacag cattatata 
      taaataacg attatacat aattatatt ttattattg 
      gacttgttt aaatttgac tatgattac gtaactgag 
      cggcggctg tgctgcagc acctaccct gttattcat 
      tattttgga tgtgtttag tgttgtctt cctttcagt 
      gagtgttca agctacgca ttaccaaaa aacataaaa 
      aaagataaa gtgaatttt aaaataata acatagtaa 
      ctgttaata ttttataca taatttaaa atacgttat 
      ttggagagc tcgttttgt tggacattg tgataggca 
      tccaagcca tgttactgt tgctaggga cgttcctag 
      accttatta gtaatagca ttcatactt gattattgt 
      caattacac cccgccact aaattaaaa gaaatgaac 
      aaattgaca gacaaaaca ttatttaat tacacagat 
      attttttgt acattactg gagttttga aatatatct 
      atatatatt aaaataaaa gtataattt ctat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY