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Gene information
General information
TC010451 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7473500..7478289
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010451
    iBeetle screen iB_10021

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): less than 30%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): less than 30%
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    head capsule structure irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    antenna scapus broader - Penetrance (percentage of animals/eggs on slide): less than 30%
    antenna antennal flagellum not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs size decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    legs tibiotarsus potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    legs femur potentially fused - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen posterior constricted - Penetrance (percentage of animals/eggs on slide): less than 30%
    posterior terminus potentially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010451 RA
      MESNTPSES GKKIVLENL SKEELVKRC ERLLILAQK 
      AKQAKDALQ EENNELKNR LKSESGDAT TAIIENLTQ 
      QKLQFVTTI EDLKSQNAA LVNKVQNCT QDARLCEEK 
      DRLESENIS YKRQIKRLT DENEQLITH LESLEKQLE 
      ECKNLGLEQ QNQLLMLEE ANQKLRSDD TSDKSAELQ 
      TMLSISLEE VKKLQAENN TMREKIDVL KTNLGEKDK 
      NILTIGQEL QEKLKLVED LEKIKNESR ENVELVEKL 
      KGRLKFYHS KVVKLAGNV KVLKENKNE VLRLLKTYS 
      HQVEEWKEQ LGVASKKLC QEISDIKVQ NSEYHKEIE 
      GLKESNAKY LKQLEAHKT DDEKSKELG EFERISKEH 
      KKLIEENQR LLQLQLDYQ QTVETLTVE KESLLENLS 
      QLEERHQEE IKVFDDQSK KDTIEIKNR IEENEKICI 
      EYRKVISKL EEDLDLNLR ELNDAKQKN YSLQEALEH 
      SKETERQIA DKTSEEILK LVQENAQLV EMNRKLQTT 
      RDNCKQVEC QTEDSTNSE LEEVVSGLK RENSELLAE 
      MNEMNQALK ERGETISKL QSHCEEVMK KLQLYETQA 
      NKNVDNISQ KEEIIKKLE DEIERLKAT SDLNSNTSE 
      ISDLKNEIE NLKERLNSN LDSSYADSE CMSTSTISR 
      TEESNRMKD LEGSWEERY GKLRNLAVK LKGKVRDLS 
      NELVKEQNE KNDIQQKCA SNVKTIATL QAQCDKLQD 
      ELDENKKQI KEYSARLDS TLAEVSKDK QKLSENEEI 
      ITILKKELE ELNQEKVTT EAWKKQISG KVSTFRKEL 
      EANKVLKKE YEAKIAKLT SDLEAKEQA LKAESEYHQ 
      QTKNSLEHS NIERKKNSV LNLEMQDYE RSVKELSQK 
      LEKKQEEIN KLKSQLESQ KSTTNAVRE QNKLLEERA 
      EEMKSEVGA TQAEIIACK KQISDLESV MSSKENKIH 
      DLTQILETV RSENEELST ELSKVIAEH QKVNTSLKS 
      EKENLKGQI LGLQQTLRD VQDALKLKE DELKIVQND 
      YENYKVRAQ SVLRQNQSR DVGLEEKLS EEVSALGVK 
      CEALTAELN KCKTVVDDL KKENEALVH EKSEVLTKY 
      EDVLSEVED LKSLYDQLT NKHQQTMIE HSETVRSLK 
      VHAETLAQC YRQQLSEQE VRHNREIIE LQSKIEKAP 
      SPELQLPPL PMHREEGEG SESTENTSK NAMHPVPLE 
      RLLGSDNDQ EIVFLKKQL SEEESKVVH LTALLADTE 
      QDLAKHAQM NKLLKEEIR RQQRSVERE KHAENLEYL 
      KNVVFKFVT LHSGDERSR LVPVLNTIL KLSPEETQK 
      LNLVARGDP GIRGWTNYL PLWPSPSKP
    CDS sequence
    • >TC010451 RA
      atggaatcg aatacgcca tccgaaagt ggcaagaaa 
      atcgttttg gagaattta tcgaaagag gaattggtt 
      aaacggtgc gaacggctt ttgattctc gcccagaag 
      gccaaacag gcgaaggac gcgctccag gaggaaaat 
      aacgagctg aaaaatagg ctaaaatcg gaatctgga 
      gatgccaca actgcaatt atcgaaaat ttgacccaa 
      cagaagttg cagtttgtt accacgatt gaagacttg 
      aagagccaa aatgccgct ttggtgaat aaagtgcaa 
      aactgcacg caagacgca cgtctgtgt gaggagaag 
      gataggctt gaaagcgag aatatttcg tataaaagg 
      caaatcaag cgtttgacg gatgaaaat gagcagctg 
      attacgcat ctggagtct ctggaaaag caactcgaa 
      gagtgcaaa aacctggga cttgaacag caaaatcag 
      ctgctgatg ttggaagag gcgaatcaa aagttacgg 
      agtgatgac acaagtgat aagtctgca gagttgcag 
      acgatgctt agtattagt ttagaggaa gttaagaag 
      ttgcaagct gagaataac acaatgagg gagaaaatt 
      gacgtgctc aagacgaat ctgggggag aaggataag 
      aacattcta acgataggc caagagctg caggaaaaa 
      ttgaaattg gttgaggat ttagaaaaa atcaaaaac 
      gaatcaaga gaaaatgtg gagttggtt gagaaactt 
      aaagggaga ctgaaattt tatcactca aaagttgta 
      aaactggcc ggtaatgtg aaggttttg aaggaaaat 
      aaaaacgag gttttgcgt ttgctcaaa acgtactca 
      catcaagtg gaagaatgg aaagaacag ttgggtgta 
      gcttcaaaa aagttgtgc caagaaatt agtgatatc 
      aaagtgcag aactctgaa tatcacaag gaaattgaa 
      ggtttgaaa gaaagtaat gcaaaatat ctgaaacaa 
      cttgaggca cataaaacc gacgacgag aaaagtaaa 
      gaacttggc gagtttgag agaatttcc aaagaacat 
      aagaaattg attgaagaa aatcaaaga ttgttacaa 
      cttcaactt gactaccag caaactgtt gaaacgctc 
      actgttgaa aaggagagt ctgcttgag aatttgtcg 
      caattggag gaaagacat caggaagaa atcaaagtg 
      tttgacgac caaagcaag aaagacaca attgagatc 
      aagaacaga atcgaagaa aatgaaaaa atttgcatt 
      gaataccga aaagtcatc tccaagctg gaggaggat 
      ctagattta aacttgagg gagctaaat gatgcgaag 
      cagaagaat tacagtctt caagaagca cttgaacat 
      tcaaaagaa acggagagg caaattgct gataaaacc 
      agtgaagaa atacttaaa ctagttcaa gaaaatgcc 
      cagttggtg gaaatgaac cgaaaattg caaaccacc 
      cgtgataac tgcaaacaa gtggaatgc caaactgag 
      gattcaaca aacagcgaa ctggaagaa gtcgtgtca 
      ggtttgaag agagaaaat tccgaactt ttggccgaa 
      atgaacgaa atgaatcaa gcactgaaa gaaagagga 
      gagactatt tccaaattg caatctcac tgcgaagaa 
      gttatgaaa aaattgcaa ttgtatgag acacaagcc 
      aacaaaaac gttgacaac atatcccaa aaggaggaa 
      ataattaaa aaacttgaa gacgaaatc gagcgactt 
      aaagccacg agtgactta aactcaaac acctccgaa 
      atcagtgat ttgaaaaac gaaattgag aatttgaaa 
      gaacggtta aactcgaat cttgattca agttacgcc 
      gatagcgag tgcatgtcc acgtcgaca atttcaaga 
      acggaggaa tcaaacaga atgaaagat ctggaaggg 
      tcttgggag gaaagatac ggaaaactg cgaaacttg 
      gccgtgaaa ctgaaaggg aaagttcgt gacttgtcc 
      aacgaattg gtgaaggag caaaacgag aagaacgat 
      attcagcaa aaatgtgcc agtaacgtg aaaaccatc 
      gcaactttg caagcccag tgcgataaa ttacaagac 
      gaattggac gaaaacaaa aaacaaata aaggaatat 
      tccgcccga ttggattcg acccttgct gaagtttcc 
      aaagataag caaaaactg agcgaaaac gaggagata 
      atcaccata ctcaagaaa gaattggaa gagttaaac 
      caagagaaa gtcacgact gaagcctgg aagaagcaa 
      ataagcggc aaagtttcg acgtttaga aaggaactc 
      gaagcgaat aaggttttg aagaaggaa tacgaggcc 
      aaaatcgcc aaactcacc tcggacttg gaggccaaa 
      gaacaggcg ctcaaggcc gagagcgag taccaccaa 
      caaacgaaa aattccctt gaacattcc aatatcgaa 
      cgcaagaaa aactccgtc cttaacctg gaaatgcaa 
      gactacgag aggagtgtc aaggagttg tcccagaaa 
      ttggaaaag aagcaagaa gaaatcaac aagttgaaa 
      agccagctc gagtcgcag aaaagcaca acaaatgcg 
      gtccgcgaa cagaacaag cttttagaa gaacgagcg 
      gaagagatg aagagcgaa gtcggagca acacaggct 
      gaaattatc gcttgtaag aagcaaatt tctgacttg 
      gaatcggtc atgtcgagt aaggagaac aaaattcac 
      gatttgacc caaattttg gaaaccgtg cgttcggaa 
      aatgaggaa ctgtcgact gagttatcc aaagtcatt 
      gccgagcat cagaaagtt aacacaagc ctgaaaagc 
      gagaaagaa aatttgaaa gggcaaatt ctggggctg 
      cagcagact ttgagagac gtccaagat gcgctaaaa 
      ctgaaggaa gacgaactg aaaatcgtg caaaatgat 
      tacgaaaat tacaaggtt cgggcgcag agtgtcttg 
      agacagaac cagagtcgc gatgttggt ctcgaggaa 
      aaattgtcg gaagaagtg tcagctctg ggggttaag 
      tgtgaggct ctcactgcg gagctaaac aagtgcaaa 
      actgttgtt gacgatttg aagaaggaa aatgaagca 
      ttggttcac gaaaaatcg gaagtcttg accaaatat 
      gaagatgtg ttgagcgaa gttgaggac cttaaatcg 
      ctctacgac caactaact aacaagcat cagcagacc 
      atgatagaa cacagcgag actgtcagg agtttaaag 
      gtccatgcc gagacgttg gcccagtgc taccgccag 
      cagttgtca gagcaggaa gtccgtcac aaccgggaa 
      ataatcgaa ctgcaaagc aaaatcgag aaagcccct 
      tctcccgag ttgcaactg ccgcctctg ccgatgcat 
      cgcgaggaa ggagaaggg tccgaaagt accgaaaat 
      acgagtaaa aatgccatg catcccgta cctttagaa 
      agattgtta ggtagcgac aatgaccag gaaattgtc 
      tttttgaaa aaacagcta agtgaggag gaatctaaa 
      gtggtccac ttgaccgca ttgctggct gatacggag 
      caggatttg gcgaagcac gcccagatg aataaactg 
      ctgaaagag gagataagg agacaacag aggagcgtc 
      gagagggag aaacatgcg gaaaatttg gagtatttg 
      aaaaatgta gtttttaag tttgtgact ctgcacagc 
      ggggacgag agaagtcgc ctggtgcca gtattgaac 
      acgattttg aaactgagt cctgaagag acccagaaa 
      cttaatctg gtggctcgg ggcgacccg ggcattcgg 
      gggtggacc aactacctt cctttgtgg ccatcaccg 
      tcgaaacca taa
    mRNA sequence
    • >TC010451 RA
      attgatgtg tcaaaataa taaaaaatt attaaaata 
      caatgtaat tgctaatta cttggaaat tacaataca 
      atagattaa gtaactatc gagatggaa tcgaatacg 
      ccatccgaa agtggcaag aaaatcgtt ttggagaat 
      ttatcgaaa gaggaattg gttaaacgg tgcgaacgg 
      cttttgatt ctcgcccag aaggccaaa caggcgaag 
      gacgcgctc caggaggaa aataacgag ctgaaaaat 
      aggctaaaa tcggaatct ggagatgcc acaactgca 
      attatcgaa aatttgacc caacagaag ttgcagttt 
      gttaccacg attgaagac ttgaagagc caaaatgcc 
      gctttggtg aataaagtg caaaactgc acgcaagac 
      gcacgtctg tgtgaggag aaggatagg cttgaaagc 
      gagaatatt tcgtataaa aggcaaatc aagcgtttg 
      acggatgaa aatgagcag ctgattacg catctggag 
      tctctggaa aagcaactc gaagagtgc aaaaacctg 
      ggacttgaa cagcaaaat cagctgctg atgttggaa 
      gaggcgaat caaaagtta cggagtgat gacacaagt 
      gataagtct gcagagttg cagacgatg cttagtatt 
      agtttagag gaagttaag aagttgcaa gctgagaat 
      aacacaatg agggagaaa attgacgtg ctcaagacg 
      aatctgggg gagaaggat aagaacatt ctaacgata 
      ggccaagag ctgcaggaa aaattgaaa ttggttgag 
      gatttagaa aaaatcaaa aacgaatca agagaaaat 
      gtggagttg gttgagaaa cttaaaggg agactgaaa 
      ttttatcac tcaaaagtt gtaaaactg gccggtaat 
      gtgaaggtt ttgaaggaa aataaaaac gaggttttg 
      cgtttgctc aaaacgtac tcacatcaa gtggaagaa 
      tggaaagaa cagttgggt gtagcttca aaaaagttg 
      tgccaagaa attagtgat atcaaagtg cagaactct 
      gaatatcac aaggaaatt gaaggtttg aaagaaagt 
      aatgcaaaa tatctgaaa caacttgag gcacataaa 
      accgacgac gagaaaagt aaagaactt ggcgagttt 
      gagagaatt tccaaagaa cataagaaa ttgattgaa 
      gaaaatcaa agattgtta caacttcaa cttgactac 
      cagcaaact gttgaaacg ctcactgtt gaaaaggag 
      agtctgctt gagaatttg tcgcaattg gaggaaaga 
      catcaggaa gaaatcaaa gtgtttgac gaccaaagc 
      aagaaagac acaattgag atcaagaac agaatcgaa 
      gaaaatgaa aaaatttgc attgaatac cgaaaagtc 
      atctccaag ctggaggag gatctagat ttaaacttg 
      agggagcta aatgatgcg aagcagaag aattacagt 
      cttcaagaa gcacttgaa cattcaaaa gaaacggag 
      aggcaaatt gctgataaa accagtgaa gaaatactt 
      aaactagtt caagaaaat gcccagttg gtggaaatg 
      aaccgaaaa ttgcaaacc acccgtgat aactgcaaa 
      caagtggaa tgccaaact gaggattca acaaacagc 
      gaactggaa gaagtcgtg tcaggtttg aagagagaa 
      aattccgaa cttttggcc gaaatgaac gaaatgaat 
      caagcactg aaagaaaga ggagagact atttccaaa 
      ttgcaatct cactgcgaa gaagttatg aaaaaattg 
      caattgtat gagacacaa gccaacaaa aacgttgac 
      aacatatcc caaaaggag gaaataatt aaaaaactt 
      gaagacgaa atcgagcga cttaaagcc acgagtgac 
      ttaaactca aacacctcc gaaatcagt gatttgaaa 
      aacgaaatt gagaatttg aaagaacgg ttaaactcg 
      aatcttgat tcaagttac gccgatagc gagtgcatg 
      tccacgtcg acaatttca agaacggag gaatcaaac 
      agaatgaaa gatctggaa gggtcttgg gaggaaaga 
      tacggaaaa ctgcgaaac ttggccgtg aaactgaaa 
      gggaaagtt cgtgacttg tccaacgaa ttggtgaag 
      gagcaaaac gagaagaac gatattcag caaaaatgt 
      gccagtaac gtgaaaacc atcgcaact ttgcaagcc 
      cagtgcgat aaattacaa gacgaattg gacgaaaac 
      aaaaaacaa ataaaggaa tattccgcc cgattggat 
      tcgaccctt gctgaagtt tccaaagat aagcaaaaa 
      ctgagcgaa aacgaggag ataatcacc atactcaag 
      aaagaattg gaagagtta aaccaagag aaagtcacg 
      actgaagcc tggaagaag caaataagc ggcaaagtt 
      tcgacgttt agaaaggaa ctcgaagcg aataaggtt 
      ttgaagaag gaatacgag gccaaaatc gccaaactc 
      acctcggac ttggaggcc aaagaacag gcgctcaag 
      gccgagagc gagtaccac caacaaacg aaaaattcc 
      cttgaacat tccaatatc gaacgcaag aaaaactcc 
      gtccttaac ctggaaatg caagactac gagaggagt 
      gtcaaggag ttgtcccag aaattggaa aagaagcaa 
      gaagaaatc aacaagttg aaaagccag ctcgagtcg 
      cagaaaagc acaacaaat gcggtccgc gaacagaac 
      aagctttta gaagaacga gcggaagag atgaagagc 
      gaagtcgga gcaacacag gctgaaatt atcgcttgt 
      aagaagcaa atttctgac ttggaatcg gtcatgtcg 
      agtaaggag aacaaaatt cacgatttg acccaaatt 
      ttggaaacc gtgcgttcg gaaaatgag gaactgtcg 
      actgagtta tccaaagtc attgccgag catcagaaa 
      gttaacaca agcctgaaa agcgagaaa gaaaatttg 
      aaagggcaa attctgggg ctgcagcag actttgaga 
      gacgtccaa gatgcgcta aaactgaag gaagacgaa 
      ctgaaaatc gtgcaaaat gattacgaa aattacaag 
      gttcgggcg cagagtgtc ttgagacag aaccagagt 
      cgcgatgtt ggtctcgag gaaaaattg tcggaagaa 
      gtgtcagct ctgggggtt aagtgtgag gctctcact 
      gcggagcta aacaagtgc aaaactgtt gttgacgat 
      ttgaagaag gaaaatgaa gcattggtt cacgaaaaa 
      tcggaagtc ttgaccaaa tatgaagat gtgttgagc 
      gaagttgag gaccttaaa tcgctctac gaccaacta 
      actaacaag catcagcag accatgata gaacacagc 
      gagactgtc aggagttta aaggtccat gccgagacg 
      ttggcccag tgctaccgc cagcagttg tcagagcag 
      gaagtccgt cacaaccgg gaaataatc gaactgcaa 
      agcaaaatc gagaaagcc ccttctccc gagttgcaa 
      ctgccgcct ctgccgatg catcgcgag gaaggagaa 
      gggtccgaa agtaccgaa aatacgagt aaaaatgcc 
      atgcatccc gtaccttta gaaagattg ttaggtagc 
      gacaatgac caggaaatt gtctttttg aaaaaacag 
      ctaagtgag gaggaatct aaagtggtc cacttgacc 
      gcattgctg gctgatacg gagcaggat ttggcgaag 
      cacgcccag atgaataaa ctgctgaaa gaggagata 
      aggagacaa cagaggagc gtcgagagg gagaaacat 
      gcggaaaat ttggagtat ttgaaaaat gtagttttt 
      aagtttgtg actctgcac agcggggac gagagaagt 
      cgcctggtg ccagtattg aacacgatt ttgaaactg 
      agtcctgaa gagacccag aaacttaat ctggtggct 
      cggggcgac ccgggcatt cgggggtgg accaactac 
      cttcctttg tggccatca ccgtcgaaa ccataatta 
      ttaattgtt atgtaaata ttgctaaat aaattattt 
      cttattcg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY