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Gene information
General information
TC010427 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007420.3: 7347713..7349393
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC010427
    iBeetle screen iB_10019

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC010427 RA
      MSSSATLIS QDVEDYNES ENTDDLEQT LVLAAQDAD 
      ARSNELQTD TSYREEVEE EVAQPEPQS TSSYKAKRK 
      HEDEEPAPK HLKTNEGQA VGRTSEGYA KVVATHYNL 
      LEEKGLDER SKSRIVYMR NFHNWIKSM LINEYLTKI 
      KDGKKHNAP VRVLDMCCG KGGDLLKWR KGNITHLIC 
      SDIASVSLD QCRSRYNDM KERSSRERN GGNIYSIEY 
      IAGDCSRVR LREKYTDPS MKLDLVSCQ FAFHYSFES 
      LPQAECMIR NASECLQPG GFFIGTIPD ANDLIARAR 
      RADANTFGN DVYQVHFDC DVNKPPLFG AKYNFHLDG 
      VVDCPEFLV HFPTLIKLA RKYGLKFVK KEKFYDYYE 
      QMKNEGRQL LINMKSLET YPPYDNTPL LGTDPGDYI 
      HAEEFLKKE GKNGKIGTL SKSEWEASS LYMLFVFEK 
      VKNTWNTDG TPVYDI
    CDS sequence
    • >TC010427 RA
      atgtcctca agtgcaacg ttaatttcc caagacgtg 
      gaggactac aacgaatcc gaaaacacc gatgatttg 
      gaacaaact ttagtatta gcggctcaa gatgcggac 
      gcacgaagt aacgagctt caaacagac accagttac 
      agggaagag gtcgaggaa gaagttgca cagccagaa 
      ccccaatca acaagcagt tacaaagca aaacgaaaa 
      cacgaggac gaagaaccg gccccgaaa cacttgaaa 
      acaaacgag gggcaagcc gttggccga acgagtgaa 
      gggtatgcc aaggtggtg gcgacccat tataacctc 
      ttggaggaa aaaggtctg gatgagcgg tcaaaatcg 
      cgcatagtt tacatgagg aacttccat aattggatc 
      aagagtatg ctgataaac gaatatttg acgaaaatt 
      aaagacggg aagaagcac aacgcgcct gtgcgcgtg 
      ttggacatg tgttgcggc aaagggggc gacctcctc 
      aagtggcgg aaaggcaac atcacccat ttgatttgc 
      agcgacatt gcttccgtc tctttggac caatgtaga 
      tcgcgctac aacgacatg aaagagcgg agcagtcgc 
      gagcgcaac gggggaaac atctactcc atcgagtac 
      atagctggg gactgttcc cgcgtaaga ctgcgcgaa 
      aaatacacc gacccttcc atgaagctc gacctggtc 
      agctgccag tttgctttt cactacagt ttcgagagt 
      ttgccccaa gctgagtgc atgatacgt aacgcctcc 
      gagtgtctc cagcctggg ggcttcttc attgggaca 
      ataccagac gccaatgat ctcattgcg agggcgcgg 
      agagccgac gcaaacacg ttcggaaac gacgtctat 
      caagtgcat ttcgactgt gatgtaaac aaaccaccg 
      ctttttggt gctaaatac aattttcat ctggacggc 
      gtggtggat tgtccagag tttttggtt cattttcct 
      acgttaatc aaattagcc agaaagtat ggcttgaaa 
      tttgtcaaa aaggaaaag ttttacgat tattatgaa 
      caaatgaaa aatgaggga agacagctg cttattaat 
      atgaaaagt cttgaaacg tatcctcct tacgacaat 
      accccactg ctggggact gatcctggg gattatatt 
      catgctgag gaatttttg aagaaagag ggcaaaaat 
      ggaaaaatt gggacgttg tccaagtca gagtgggag 
      gcttcttcc ttgtatatg ctgtttgtc tttgaaaag 
      gtgaagaat acgtggaat acggatggc acacctgtt 
      tatgatata taa
    mRNA sequence
    • >TC010427 RA
      aaattaatc aattaaaat aaaatacgt tatggatac 
      gtaacgtgg tagcgtcca actttgcgc ttgttgtga 
      ctttgggca catttcagc gtttgacgt acaattgac 
      agattgggg ttagtgaac ccttactcc aattttcaa 
      agaaagtta tttattttt gtaaacaca aacgtgtga 
      aggccaaaa gtacaataa taacgttaa gtgtgaaaa 
      tgtcctcaa gtgcaacgt taatttccc aagacgtgg 
      aggactaca acgaatccg aaaacaccg atgatttgg 
      aacaaactt tagtattag cggctcaag atgcggacg 
      cacgaagta acgagcttc aaacagaca ccagttaca 
      gggaagagg tcgaggaag aagttgcac agccagaac 
      cccaatcaa caagcagtt acaaagcaa aacgaaaac 
      acgaggacg aagaaccgg ccccgaaac acttgaaaa 
      caaacgagg ggcaagccg ttggccgaa cgagtgaag 
      ggtatgcca aggtggtgg cgacccatt ataacctct 
      tggaggaaa aaggtctgg atgagcggt caaaatcgc 
      gcatagttt acatgagga acttccata attggatca 
      agagtatgc tgataaacg aatatttga cgaaaatta 
      aagacggga agaagcaca acgcgcctg tgcgcgtgt 
      tggacatgt gttgcggca aagggggcg acctcctca 
      agtggcgga aaggcaaca tcacccatt tgatttgca 
      gcgacattg cttccgtct ctttggacc aatgtagat 
      cgcgctaca acgacatga aagagcgga gcagtcgcg 
      agcgcaacg ggggaaaca tctactcca tcgagtaca 
      tagctgggg actgttccc gcgtaagac tgcgcgaaa 
      aatacaccg acccttcca tgaagctcg acctggtca 
      gctgccagt ttgcttttc actacagtt tcgagagtt 
      tgccccaag ctgagtgca tgatacgta acgcctccg 
      agtgtctcc agcctgggg gcttcttca ttgggacaa 
      taccagacg ccaatgatc tcattgcga gggcgcgga 
      gagccgacg caaacacgt tcggaaacg acgtctatc 
      aagtgcatt tcgactgtg atgtaaaca aaccaccgc 
      tttttggtg ctaaataca attttcatc tggacggcg 
      tggtggatt gtccagagt ttttggttc attttccta 
      cgttaatca aattagcca gaaagtatg gcttgaaat 
      ttgtcaaaa aggaaaagt tttacgatt attatgaac 
      aaatgaaaa atgagggaa gacagctgc ttattaata 
      tgaaaagtc ttgaaacgt atcctcctt acgacaata 
      ccccactgc tggggactg atcctgggg attatattc 
      atgctgagg aatttttga agaaagagg gcaaaaatg 
      gaaaaattg ggacgttgt ccaagtcag agtgggagg 
      cttcttcct tgtatatgc tgtttgtct ttgaaaagg 
      tgaagaata cgtggaata cggatggca cacctgttt 
      atgatatat aagtagtaa accaaatgt acagtttta 
      gtacaagaa gactattat ttattgaaa taataaaac 
      tggtgactt tta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY